Aquatic Animal Health Subprogram: Identifying the cause of Oyster Oedema Disease (OOD) in pearl oysters (Pinctada maxima), and developing diagnostic tests for OOD
Pearl oyster farming is one of Australia's most successful aquaculture industries, with substantial scope for future growth. Recent data suggest that the pearling industry generates approximately $100 m pa and provides significant employment opportunities in remote areas of Australia. Despite its remarkable success, the viability of Australian P. maxima pearling is compromised by a fatal disease (oyster oedema disease, OOD). If OOD is not controlled, the industry is unlikely to develop as efficiently and cost effectively as required. In some areas, the disease is making it difficult to restock growing areas with hatchery reared spat, and it is significantly decreasing the number of oysters available to produce pearls. OOD also appears to be impacting wild P. maxima populations. The proposed project is the first step in developing an integrated management strategy to control OOD in the Australian pearling industry. Some progress has been made in studying the disease. We suspect that OOD is initiated by an infectious virus associated with secondary bacterial infection. But we still do not understand: 1. the precise causative agent(s) of OOD, or, 2. how to provide early diagnosis of the disease These gaps in our knowledge mean that industry does not have sufficient information to establish effective management strategies that can control OOD. Most importantly, we need diagnostic methods to detect the onset of disease before catastrophic outbreaks occur. Such tests could be used to identify disease-free broodstock for hatcheries, find areas where OOD is less prevalent, determine environmental factors that might be associated with infection, and manage the translocation of OOD-affected oysters. Our project addresses these needs in order to identify management practices that can be used to control OOD and secure the Australian pearling industry.
1. REVISED PROJECT OBJECTIVE 1. Identify the presence of nucleotide sequences associated with OOD affected oysters using a next generation sequencing approach
1. SUPERCEDED OBJECTIVE 1: Identify the presence of any pathogens associated with OOD-affected oysters using a next generation sequencing approach.
2. SUPERCEDED OBJECTIVE 2: Investigate the association of the pathogens detected by next generation sequencing with the severity of disease in P. maxima using a combination of quantitative (q) PCR, pathology and histopathology (including in situ hybridisation).
2. REVISED PROJECT OBJECTIVE 2: 2. Use quantitative real time PCR to measure the prevalence of these nucleotide sequences in a broad range of oyster samples
3. SUPERCEDED OBJECTIVE 3: Develop diagnostic tests that can detect and identify the causative infectious agent(s) of OOD