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Title:

Aquatic Animal Health Subprogram: Development of stable positive control material and development of internal controls for molecular tests for detection of important endemic and exotic pathogens

Project Number:

2014-002

Organisation:

CSIRO Oceans and Atmosphere Hobart

Principal Investigator:

Nick Moody

Project Status:

Current

FRDC Expenditure:

$172,185.00

Program(s):

Environment

Objectives

1. Produce quantified synthetic RNA positive control material for conventional and real-time RT-PCR assays, available on request.

2. Produce quantified plasmid DNA positive control material for conventional and real-time PCR assays, available on request.

3. Optimised universal internal control based on plant viral RNA and DNA and/or species-specific genes for use in molecular assays developed and implemented

4. Technology transferred and adopted by participating laboratories.