Atlantic Salmon Aquaculture Subprogram: molecular genetic tools for the Tasmanian Atlantic salmon industry – development and application
CSIRO Oceans and Atmosphere Hobart
Nick G. Elliott
The efficiency and effectiveness of selective breeding programs can be greatly enhanced through the use of DNA technology. The application of such technology will be used for pedigree information and identification of markers for economic traits leading to marker-assisted selection. Although various international laboratories and CSIRO have developed a bank of molecular markers for Atlantic salmon, the potential of these and other nuclear DNA markers such as AFLPs need evaluating. Through this project we would be able to formally collaborate on an international mapping project for salmonids. This would give the local industry access to far more markers and genetic information than would be possible if we worked in isolation. The extent of genetic variation within the Tasmanian population with respect to overseas endemic populations remains unclear. In time it may be considered advantageous to import new genetic material to enhance various characteristics of the local population. However, at present that is unlikely and the genetic status of the population, and of the effectiveness of breeding protocols, needs to be better understood. Significant progress was made with this and the search for sex differences (project 96/347), but funding is required to further the research.
1. To further develop and apply molecular markers for use in industry breeding programs.
2. To genotype selected broodstock with a suite of microsatellite markers to enable efficient pedigree analysis of progeny.
3. To compare microsatellite DNA variation from archival scale samples from the progenitor Canadian population with past and current cohorts of the Tasmanian population.
Principal Investigator: Nicholas G. Elliott, Bronwyn H. Innes, James W. Wynne & Mathew T. Cook