1. To develop the use of microsatellites for the genetic analysis of yellowfin tuna

2. To determine the genetic relationships of yellowfin tuna form the Coral Sea To those from the east coat of Australia and to those from other Western Pacific fisheries

3. To develop techniques for estimation of oxygen 18/16 isotopic ratios in otoliths using SIMS microprobe

4. Using analysis of otolith chemistry and isotopic ratios examine the hypothesis that all recruits to the ECTUNA fishery are derived from spawning grounds in the Coral Sea

5. In the event of rejection of the null hypothesis that there is a single panmictic WP stock, use otolith chemical data collected from yellowfin collected throughout WP to estimate the proportion of immigrant recruits and determine their most likely origin