Project number: 2001-621
Project Status:
Completed
Budget expenditure: $162,049.00
Principal Investigator: Nicky Buller
Organisation: Murdoch University
Project start/end date: 13 Jan 2002 - 18 Jul 2005
Contact:
FRDC

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Objectives

1. Develop a sensitive and specific molecular diagnostic test for the detection of aphanomyces invadens, based on the polymerase chain reaction (PCR) for use with fresh or dead tissue samples.
2. Develop a sensitive and specific molecular diagnostic test for the detection of aphanomhyces astaci, based on the PCR for use with fresh or dead tissue samples.
3. Develop a rapid molecular diagnostic test for the detection of aphanomyces invadens, based on the technique of fluorescent in-situ hybridisation (FISH), which allows visualization of the fungus direct from lesion smears or culture material within one hour.
4. Develop rapid molecular diagnostic test for the detection of aphanomyces astaci, based on the technique of fluorescent in situ hybridisation (FISH), which allows visualisation of the fungus direct from lesion smears or culture material within one hour.
5. Transfer of this technology in the form of a kit for initial distribution and evaluation to selected laboratories.
6. Write up Australian Standard Diagnostic Technique for EUS, and update the ASDT for Crayfish Plague based on the above tests and in the format supplied by AFFA.

Final report

ISBN: 0-646-44068-3
Author: Nicky Buller

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