Project number: 2003-067
Project Status:
Completed
Budget expenditure: $42,708.69
Principal Investigator: Rory McAuley
Organisation: Department of Primary Industries and Regional Development (DPIRD) WA
Project start/end date: 29 Jun 2003 - 30 Jun 2006
Contact:
FRDC

Need

The continued black-market in body parts from vulnerable and endangered sharks, has the potential to cause once common species to become, at least regionally, extinct. A deterrent to the illegal capture of and trade in protected shark species is therefore essential. The sustainability of several commercially important species is likely to be placed under increasing threat without better means of controlling the exploitation of individual species. The development of legally defensible protocols and a dataset of DNA reference samples will provide the WA Department of Fisheries and other regulatory authorities with the tools necessary for enforcing both existing and future management and conservation regulations.

The need for improved species composition data, particularly from bycatch fisheries is recognised in the National Plan Of Action for the conservation and management of sharks (NPOA-sharks). The proposed DNA dataset will provide the basis for fishery management authorities to validate the accuracy of logbook data and determine species composition of catches for which there are currently no catch records (eg. confiscated illegal catches).

Objectives

1. Establish sampling protocols (ensuring methodology will be suitable for evidentiary purposes) and collect reference samples
2. Establish ‘legally robust’ DNA processing protocols and process reference samples
3. Establish a secure DNA ‘fingerprint’ database for WA shark species to act as both a provider of evidence and a deterrent to illegal fishing activity

Final report

ISBN: 1-877098-76-0
Author: Rory McAuley
Final Report • 2006-01-11 • 1.66 MB
2003-067-DLD.pdf

Summary

The capability to identify individual shark species from processed body parts is necessary for the WA Department of Fisheries to ensure the compliance of all WA fisheries with both existing protected species regulations and proposed new management measures for commercially important shark species. This project established a reference database of genetic profiles or ‘fingerprints’ for nine of Western Australia’s protected and commercially important shark species. Fingerprints from a 10th species, the shortfin mako (Isurus oxyrinchus) could not be obtained due to probable primer sequence mismatching. The database, in conjunction with an associated inspection and testing regime, will act as a significant deterrent to the trade in body parts from protected shark species and also as a source of information on levels of bycatch of commercially important shark species in non-target fisheries. Genetic material from voucher specimens was collected by the principal investigator according to documented species identification and verification procedures. Samples were securely stored in tamper-proof containers until their transfer to the Chemistry Centre (WA) for genetic analysis and profiling. Additional fingerprints were derived from samples from the WA Department of Fisheries’ shark DNA reference collection and included in the database, to ensure that as much genetic variation as possible was represented for each catalogued species. The collection, storage and transfer of each sample was documented and the integrity of each sample was verified on receipt by the project co-investigator prior to analysis. These continuity of evidence protocols were developed according to WA Police Service guidelines for forensic evidence collection to ensure the database’s suitability as a provider of legally robust evidence.

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