Project number: 2009-315.32
Project Status:
Completed
Budget expenditure: $3,340.88
Organisation: Department of Natural Resources and Environment Tasmania (NRE TAS)
Project start/end date: 30 Jun 2014 - 29 Apr 2015
Contact:
FRDC

Need

The Molecular Biology group at the Animal Health Laboratory of the Department of Primary Industries, Parks, Water & Environment, provides diagnostic and disease investigation services for aquaculture industries in Tasmania. The increasing reliance on molecular techniques for the detection and identification of disease agents in aquatic animal health is undeniable. Due to the ever increasing number and more efficient molecular techniques becoming available and the dynamic nature of newly emerging pathogens, specialist training is increasingly important for the delivery of effective and useful services to aquaculture. Presently, species identification relies heavily on biochemical tests and cellular fatty acid analysis, or 16S rDNA gene sequencing, all of which at times can be non-definitive. Multi-locus sequence analysis uses several housekeeping genes to assign a species designations, and is a technique that is easy to use, accurate, and has great discriminatory power (McTaggart et al., 2010). The multi locus sequencing skills acquired would enable us to more specifically and accurately perform species identification and determine genetic diversity among different isolates and build up a gene bank of sequences in the future.

Objectives

1. Obtain training in advanced molecular biology techniques with an emphasis on DNA sequencing and phylogenetic analysis at a known international specialist laboratory
2. Undertake training in bioinformatics programmes with an emphasis on Multilocus Sequence Analysis (MLSA) at a known international specialist laboratory

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