While the AAHTS has been in operation for 9 years, there remains a need for the continual education and training of aquatic animal health professionals to enhance their skills across a range of specialist disciplines. Based on the significant outcomes from the previous nine years of the AAHTS, the Department of Agriculture has contributed $75,000.00 (less $7,500.00 (10%) management fee, cash contribution to FRDC) to ensure that the AAHTS continues to be implemented for a further 3-year period (2019/20-2021/22) with matching funding anticipated from the FRDC Aquatic Animal Health and Biosecurity Subprogram. Thus, an application is needed to generate an agreement between CSIRO-AAHL and FRDC for continuation of AAHTS which would be administered as previously, i.e., by CSIRO-AAHL (rather than FRDC) through the FRDC Aquatic Animal Health and Biosecurity Subprogram.

Current diagnostic programs generally rely on highly -specific assays for pathogen detection. While these techniques are invaluable, they are one dimensional and do not provide detailed information critical to a disease investigation. These gaps include the inability to detect unknown pathogens and potential variants of know pathogens and provide no additional genomic or transcriptomic data. Moreover, samples must be shipped to trained personnel in a laboratory, further delaying the time to diagnosis. The MinION, on the other hand, can theoretically detect any pathogen and can potentially be deployed to the field. Moreover, the MinION can rapidly generate full-length genomes, allowing for epidemiological tracking of viral or bacterial strains in near real-time. Such rapid data, which cannot be obtained as quickly using existing methods, are vital if the intention is to intervene in an outbreak and reduce impacts on the productivity and profitability of aquaculture facilities. For example, a rapid, early diagnosis may allow mitigating actions to be taken on-farm, such as the diversion of intake water, movement restrictions of stock and the isolation of infected ponds.
These qualities make the MinION an attractive complimentary platform to fill several gaps in the data obtained during disease outbreak investigations, or routine diagnostics, and potentially for use in the field. However, results from the misuse or lack of understanding of the technology could also have adverse regulatory implications for aquaculture industries. For example, without appropriate guidelines, an inexperienced diagnostician may misinterpret a distant DNA match in a pathogen database as a significant result, this may create unwanted attention to industry and potential stock destruction or changes to disease status that are unjustified. Thus, it is critical that the MinION is evaluated at the Australian Animal Health Laboratory, and guidelines and procedures are developed for accurate diagnostic evaluations. The activities detailed in this application will establish the feasibility of using the MinION for diagnostic applications, and ensure that the data is reliably generated and interpreted appropriately.

AAHBS provides a cohesive national approach to FRDC-supported aquatic animal health R&D in Australia by providing leadership, direction and focus for health R&D and other related non-R&D activities. AAHS was renewed in 2012 based on national need, previous performance and strong stakeholder support. Key strengths include its strategic focus and the establishment of a network of aquatic animal health experts and research providers. An external review of AAHS was undertaken in 2015 and it found that the consensus among the major stakeholders was that AAHS provides an essential service for the major stakeholders. Thus, continuation of AAHS beyond 2016 on the basis of a shared financial commitment by stakeholders from industry and governments is highly desirable.

Australia’s aquatic animal sector is free from many diseases that occur overseas, providing us with a competitive advantage in both production and trade. While the number of aquatic animal species and the absolute number of aquatic animals being farmed in Australia is increasing annually, new diseases caused by emerging infectious agents (e.g. new yellow head virus genotypes, oyster oedema disease, ostreid herpesvirus, Bonamia exitiosa) continue to threaten the sustainability of significant enterprises, and the call on health services to support this expanding industry is growing.

In addition to aquaculture, aquatic animal health R&D is required for the other aquatic animal sectors, including wild-capture (c.f. Edwardsiella ictaluri in catfish), recreational (c.f. Perkinsus in oysters) and ornamental (c.f. gourami iridovirus), that share the aquatic environment. Thus health services need to be coordinated across all aquatic sectors to ensure synergy while avoiding duplication. FRDC, through AAHBS, plays a major role in addressing research needs and training in aquatic animal health. With its incumbent expertise and experience, AAHBS is able to direct these activities in the most pressing areas.