Assessing post-release survival of Southern Bluefin Tuna from recreational fishing
SCRC: Ongoing salmon and oyster research in a new Experimental Aquaculture Facility (EAF)
Seafood CRC: Aquaculture Production Innovation Hub: Phase II – communication, extension and opportunities
SCRC: RTG: “R” Basic and Design and Analysis of Experiments Workshop, The University of Western Australia, Perth
Final report
The research travel grant allowed a CRC PhD student to attend a training course on the introduction to the statistical software environment “R”, reading and handling data, graphic analysis and data output analysis, writing simple R-based functions, developing flow control structures, and use of ANOVA and split level designs. The training was conducted as an interactive face-to-face workshop at the Centre of Applied Statistics, the University of Western Australia, Perth (27 November 2012 until 30 November 2012).
The research travel grant improved the student's skills and knowledge for experimental design, analysis of research data and also advanced his technical skills in biostatistics. The student is now using R-based statistical packages to analyse the research data source, because it’s a free and extremely powerful language and software environment for statistical computing, data analysis and graphics. It has become the tool of choice for many statisticians. Understanding of R is extremely valuable for the student’s professional development, data analysis skills, and potentially improves the outcomes of the PhD project.
SCRC: Seafood CRC Combined Visiting Expert and Research Travel Grant Application: Visit by Dr. Standish K. Allen Jr. from the Virginia Institute of Marine Science to Australia and fluorescent in situ hybridisation training by CRC PhD student Penny Miller prior to Dr Allen’s visit
Final report
Fluorescent in situ hybridisation (FISH) is a genetic technique that involves fluorescently labelling chromosomes so that each can be identified individually under a high powered microscope. FISH could be an important tool for detecting the aneuploid frequency in tetraploid oyster populations. This is important because a decrease in tetraploid genetic stability could potentially reduce the efficiency of breeding programs and may have carry over impacts on the triploid commercial product.
The PhD student travelled to Canberra to work with Tariq Ezaz of the University of Canberra on troubleshooting her FISH protocol to work on Pacific Oysters. Eventually, the protocol worked, but not consistently or at a strong enough level for chromosomes to be individually identified. It was determined that, due to their small size and weak signals, fluorescently labelled microsatellites are not a reliable method for karyotyping oysters, particularly polyploids where chromosomes tend to overlap. A different probe (PNA) was also trialled. Again this was inconsistent, but the signals were stronger than the microsatellites. This probe is worth mapping and further investigation, however, time, money and sampling constraints prevented any additional study.
SCRC: Seafood CRC Research Travel Grant: Targeted meetings with key research providers and industry personnel in the UK relevant to CRC projects 2011/703 and 2011/735
Final report
This trip involved targeted meetings with key research and industry personnel to consolidate the existing relationship and secure ongoing research support for two Seafood CRC student projects as well as to advance the research agendas developed as part of those projects and establish ongoing international research partnerships.
These projects included “An evaluation of the options for expansion of salmonid aquaculture in Tasmanian waters”, and “Tracking methyl mercury contamination pathways in key commercially and recreationally fished species”. The travel grant supported meetings with researchers and industry in the UK, including representatives from the University of Plymouth, Marine Institute at the University of Plymouth, Marine Harvest International and the University of St Andrews.