Experimental and clinical studies of mannitol in the treatment of ciguatera
Final report
Project products
Production of antibodies against toxins involved in ciguatera fish poisoning
Final report
Sufficient pure ciguatoxin (0.5 mg of CTX-1) has been isolated from moray eel viscera to allow the immunisation of mice and screening of antibodies to ciguatoxin. It is determined that in vivo immunisation techniques were most likely to yield antibodies using related toxins as models of ciguatoxin. The model toxins used included brevetoxin, okadaic acid and tetrodotoxin. Using the same approach it was determined that the in vitro immunisation techniques investigated were inappropriate. It appears essential that ciguatoxin be conjugated to a carrier protein to allow production and screening of antibodies to ciguatoxin. The use of unconjugated toxin, either for immunisation or screening, appears inappropriate. The conjugation of ciguatoxin to a carrier protein has not been achieved despite considerable effort. Consequently, it was not possible to achieve the overall goal of the project. Until it is confirmed that ciguatoxin possesses the functional groups that allow the toxin to be conjugated to a carrier protein, work towards the development of antibodies to ciguatoxin cannot proceed.
During this project 595 potentially toxic fish samples were tested for toxin content. This included numerous samples of moray eel viscera and numerous portions of fish involved in ciguatera in Australia.
During this project two new ciguatoxins (CTX-2, 0.3 mg; CTX-3, 0. 1 mg) were also isolated from moray eel viscera. These toxins were determined to be less oxidised forms of ciguatoxin. Analysis of moray eel toxicity indicated that these fish may excrete ciguatoxin over time.
Using mouse bioassay, the efficiency of extraction of ciguatoxin was determined to be > 50% in fish flesh spiked with ciguatoxin. Mouse assay is not suitable to detect low toxicity fish flesh samples but is useful as a confirmatory assay for fish with a toxicity high enough to demonstrate moderate to severe clinical effects.
Future studies are required to resolve questions on the chemistry of ciguatoxin. These studies are presently underway. With this information the project can proceed to the production of antibodies to ciguatoxin using the procedures and protocols developed during this project.
Factors affecting the toxicity of the dinoflagellate, Gambierdiscus toxicus, and the development of ciguatera outbreaks
Final report
This project confirmed that the benthic dinoflagellate Gambierdiscus toxicus is an origin of putative ciguatoxin precursors called gambiertoxins. It was recently found in French Polynesia that gambiertoxins could be extracted from biodetritus containing wild G. toxicus and that gambiertoxins were structural analogs of ciguatoxin. Ciguatoxin is the toxin found in the flesh of ciguateric fishes. It is demonstrated - for the first time- production of gambiertoxins in strains of G. toxicus grown in culture.
The production of gambiertoxins appears to be limited to certain genetic strains of G. toxicus, with the majority of strains not producing these toxins. It is proposed that ciguatera occurs when blooms of G. toxicus strains genetically capable of producing ciguatoxin precursors (gambiertoxins) enter the marine food chain. The type of maitotoxin produced by G. toxicus was also shown to be a stable characteristic within culture strains, but variable between strains.
Disturbance to coral reef environments has been suggested as a factor in promotion of ciguatera outbreaks. However, the researchers have been unable to find direct evidence to support this hypothesis. Translocation of ciguateric strains of G. toxicus (gambiertoxin producers) may explain the sudden appearance of ciguatera in areas previously free of the disease. The ability of living G. toxicus cells to be transported, either in ship ballast water or epiphytically upon the hulls of ships, had yet to be demonstrated. G. toxicus can survive rafting upon drift algae. Translocation by ships may be a mechanism for introduction of the causative organism to sites where reef damage occurs (eg. harbour works, anchorages and shipwrecks).
G. toxicus is a common component of reef benthos in Queensland. The researchers show that G. toxicus populations in Queensland generally do not produce ciguatoxin or ciguatoxin precursors. In addition, it is demonstrated that maitotoxin or non-toxic precursors in wild G. toxicus populations are not bioconverted to ciguatoxin in fishes. The majority of G. toxicus populations in Queensland are therefore probably not involved in ciguatera.
Platypus Bay is a major source of ciguateric fishes in Queensland. G. toxicus populations which produce gambiertoxins have been found in Platypus Bay biodetritus. A monoclonal strain of G. toxicus which produces gambiertoxins in laboratory culture was also isolated from Platypus Bay. This indicated that Platypus Bay wass probably the source of toxin in ciguateric fishes caught in Platypus Bay. However, gambiertoxins were not detected in the majority of biodetrital samples collected from Platypus Bay. Gambiertoxins therefore appear to be only infrequently pulsed into Platypus Bay.
Benthic dinoflagellates other than G. toxicus are suggested as potentially capable of producing toxins associated with ciguatera. Ciguatoxin or gambiertoxins were not produced by cultures of the benthic dinoflagellates Ostreopsis siamensis and Goolia monotis isolated from Queensland. These species produced water soluble and lipid soluble toxins, respectively. However, there is no evidence for the bioaccumulation of these toxins in the flesh of fishes.
Investigation of mushiness in crabs
A cost model of the otter trawl fishery for prawns
Final report
A study of the sand crab (Portunus pelagicus) and its exploitation in a sub-tropical multi-sector fishery
A study of seagrass prawn nursery grounds and juvenile prawn populations in north Queensland
Final report
Seagrasses are of immense ecological importance in marine ecosystems. Primary production rates for seagrass beds are amongst the highest recorded for marine and terrestrial systems. They have a well documented role as animal habitat, nursery grounds, and as substrate stabilizers.
The proximity of population centres to many of the seagrass colonised estuaries and embayments of the Australian coast has catalyzed research of the effects of pollution and erosion on these marine angiosperms. Although the importance of seagrass habitat and the potential for its loss has been recognized in these studies, ecological research remains surprisingly sparse. This is particularly so in the northern regions of Australia.
In northern Queensland, seagrass-vegetated areas are important as habitats for the juveniles of commercial penaeid prawn species. The tiger prawns, Penaeus esculentus and P. semisulcatus, and the endeavour prawn, Metapenaeus endeavouri, form the major component of an otter trawl fishery for prawns in this region worth around $60 million annually.