218 results

Physical oceanographic influences on Queensland reef fish and scallops

Project number: 2013-020
Project Status:
Completed
Budget expenditure: $170,000.00
Principal Investigator: Tony J. Courtney
Organisation: Department of Primary Industries (QLD)
Project start/end date: 23 Jun 2013 - 29 Mar 2015
Contact:
FRDC

Need

There is a strong need for Queensland fishery managers to obtain a better understanding of key physical oceanographic influences on target species of commercial and recreational fisheries.

Tropical cyclones have been associated with reef fish catch rates. Coral trout (Plectropomus leopardus) catch rates typically fall after a major cyclone, while those of red throat emperor (Lethrinus miniatus) rise (see “Background” above). The effects on catchability can last several years. While the exact causal mechanism is not known, it is thought to be related to water temperature.

Nutrient-rich cold water eddies, which break from the East Australian Current and move westward onto the Queensland continental shelf are likely to affect the spat settlement, growth, abundance and catch rates of saucer scallop (Amusium balloti). Understanding these relationships may lead to improved management, assessment and forecasting of catch in these fisheries, and it may also lead to improved acceptance of quantitative stock assessment results by industry.

This proposal differs from previous abiotic studies because it focuses more on offshore, oceanic influences, rather than coastal rainfall and flow data.

Objectives

1. Review recent advances in the study of physical oceanographic influences on fisheries catch data, and describe the major physical oceanographic features that are likely to influence Queensland reef fish and saucer scallops.
2. Collate Queensland’s physical oceanographic data and fisheries (i.e., reef fish and saucer scallops) data.
3. Develop stochastic population models for reef fish and saucer scallops, which can link physical oceanographic features (e.g., sea surface temperature anomalies ) to catch rates, biological parameters (e.g., growth, reproduction, natural mortality) and ecological aspects (e.g. spatial distribution).

Developing jungle perch fingerling production to improve fishing opportunities

Project number: 2012-213
Project Status:
Completed
Budget expenditure: $482,907.00
Principal Investigator: Michael Hutchison
Organisation: Department of Primary Industries (QLD)
Project start/end date: 14 Jun 2012 - 29 Jun 2015
Contact:
FRDC

Need

Jungle perch once occurred widely in coastal Queensland rivers, from Cape York to Northern NSW. Central and southern populations have declined or become locally extinct due to dams and weirs blocking migration pathways between saltwater (where they spawn) and freshwater habitats (where they reside as juveniles and adults). Unlike barramundi, mullet and bass; jungle perch cannot persist long-term in saltwater habitats. Barriers lead to rapid local extinction. Construction of fishways on barriers in the past 10 years has created suitable conditions for the reintroduction of jungle perch. There are limited opportunities for natural recolonisation, and remnant adult populations of local strains are too few for translocation to be a practical solution. Restocking from captive bred individuals is the only option to bring back jungle perch fisheries.

Restoring wild jungle perch fisheries is a high priority for recreational fishers in Queensland. Reintroduction of self sustaining populations in rivers in south-eastern Queensland and the Mackay-Whitsunday Region will provide angling opportunities to large populations of anglers. Development of jungle perch fingerling production will also create future opportunities to further enhance Queensland's stocked impoundments and make jungle perch accessible to even more anglers.

Recent research by DEEDI has solved much of the reproductive biology of jungle perch, which can now be spawned regularly in captivity. Jungle perch larvae are much smaller (2.3 mm) than bass and barramundi larvae and establishment of first feeding has been problematic. Strategies to promote larval feeding need to be developed. The transition from larvae to fingerlings is critical for future development of jungle perch fisheries.

Objectives

1. Develop hatchery production techniques for jungle perch fingerlings
2. Successfully release jungle perch fingerlings into suitable south-eastern Queensland and Mackay-Whitsunday regional waterways
3. Communicate with anglers on the restoration of jungle perch fisheries
4. Understand environmental factors influencing post-release survival of jungle perch in rivers.
5. Develop a jungle perch production manual for fish hatcheries

Final report

ISBN: 978-0-7345-0453-1
Author: Michael Hutchison
Final Report • 2016-03-17 • 5.18 MB
2012-213-DLD.pdf

Summary

This project has for the first time demonstrated the feasibility of hatchery production of jungle perch fingerlings. The research on jungle perch production has enabled a hatchery production manual with accompanying videos to be produced. This has given private commercial hatcheries the information needed to produce jungle perch fingerlings. Several hatcheries have already indicated an interest in producing jungle perch and will be assisted to do so in 2016. Currently jungle perch are not a permitted stocking species, so cannot be sold to fish stocking groups. However, hatcheries will be able to sell fingerlings to the aquarium trade or supply grow out facilities that could produce jungle perch for human consumption. Should jungle perch become a permitted species for stocking, this will provide hatcheries with a major new product option to sell to fish stocking groups. It would also benefit anglers by providing another iconic species for impoundment stocking programs. This could have flow-on benefits to regional economies through angler tourism.

Should the pilot reintroductions of jungle perch into streams result in self-sustaining jungle perch populations, then there will be three restored jungle perch populations close to major population centres. This will create a new opportunity for anglers not normally able to target jungle perch. Since the majority of anglers who target jungle perch are catch and release fishers, angling is expected to have minimal impact on recovery of the populations.

This project led to the development of a hatchery manual for jungle perch production and to a summary brochure. In late 2014 and in 2015 researchers were able to make the first ever releases of jungle perch fingerlings back into rivers and streams within their historical range.

Keywords: Jungle perch, Kuhlia rupestris, hatchery production, restoration, fish stocking, captive breeding, larval culture, recreational fishing.

Project products

Manual • 2.19 MB
2012-213 Jungle fingerling production manual.pdf

Summary

This manual is based on the knowledge gained by researchers at the Bribie Island Research Centre (BIRC), working on developing jungle perch Kuhlia rupestris captive breeding as part of the FRDC funded project 2012/213 “Developing jungle perch fingerling production to improve fishing opportunities”. Further refinements can certainly be made to improve larval rearing and fingerling production. This manual reports on methods that have worked at BIRC to date, and perhaps more importantly, on what didn’t work. Knowledge of what has failed will help private hatchery operators avoid mistakes as they try to further refine the jungle perch production process in their own facilities.

The manual describes each of the key parts of jungle perch production, including broodstock management, spawning induction, spawning, egg and larvae management, live feed production, pond management, pond harvesting and fingerling management. The manual also includes links to video segments to demonstrate how things were done at BIRC. Videos are integral for the use of this manual. Click on the video link at the end of each production step described in this manual. It is intended that the video segments will enhance understanding of the jungle perch production process. The videos in this document are also available in the attached video folder that accompanies DVD and USB drive versions of this document and can be viewed as stand-alone files. The written document contains the majority of the technical information required, such as stocking densities, fertilising rates, feeding rates etc. The videos demonstrate the processes, which words are not always adequate to describe.

Brochure • 453.38 KB
2012-213 Jungle perch project summary brochure.pdf

Summary

This brochure outlines the achievement of the project. It is intended to provide general information on project outcomes to recreational anglers.

It summarises the results of the project.

Revolutionising fish ageing: Using Near Infrared Spectroscopy to Age Fish

Project number: 2012-011
Project Status:
Completed
Budget expenditure: $179,612.00
Principal Investigator: Julie B. Robins
Organisation: Department of Primary Industries (QLD)
Project start/end date: 3 Jun 2012 - 29 Mar 2014
Contact:
FRDC

Need

Estimates of the age structure of fish populations are central to assessing the status of fished stocks, evaluating management strategies and the impact of fishing. Determining fish age is usually done by counting growth checks in fish otoliths. This is generally a laborious, time-consuming process involving considerable preparation of the otoliths (resin-embedding, cutting and polishing) prior to ring-counting. This proposal aims to develop and validate a rapid innovative method for ageing fish based on an analysis of otoliths by NIRS. With increasing costs and, in some jurisdictions, diminishing R&D budgets, collection and ageing of representative fish samples becomes increasingly difficult. If NIRS is as efficient at determining fish age as the preliminary study suggests, it will be a breakthrough of global significance.

Commonwealth and State legislation requires that fishery resources be managed sustainably. Age-based stock assessment methods are one of the most informative tools available for assessing Australia’s fisheries. The collection of otoliths from recreational and commercial catches is undertaken across a wide range of fisheries as part of fishery monitoring and assessment programs.

This proposal addresses the need and QFRAB priority for:

• Developing innovative tools and technologies for managing Australian fisheries

• Developing more efficient, cost-effective ways of obtaining the information needed to undertake age-based fishery assessments

• More reliable fishery assessments by improving the availability and quality of age information from fish population samples

Objectives

1. Evaluate Near Infrared Spectrometry (NIRS) as a reliable, repeatable, cost-effective method of ageing fish
2. Determine the effect (if any) of otolith storage time (years/ months) on NIRS estimates of age.
3. Determine the effect of geographic location (including latitude) distribution on NIRS algorithium stability.
4. Evaluate the cost-effectiveness of ageing fish by NIRS vs. standard otolith ageing, and develop optimised fish sampling regimes with respect to ‘cost’ (defined in terms of labour, lab time, field costs, etc).

Final report

ISBN: 978 0 7345 0449 4
Authors: J.B. Robins B.B. Wedding C. Wright S. Grauf M. Sellin A. Fowler T. Saunders and S. Newman
Final Report • 2015-05-14 • 5.87 MB
2012-011-DLD.pdf

Summary

Results from the current ‘proof of concept’ study indicate that near infrared (NIR) spectra collected from fish otoliths have potential to estimate the age of Barramundi (Lates calcarifer) and Snapper (Pagrus auratus), with performance varying between species and locality of capture. A case study of hypothetical running costs suggest significant cost savings could be achieved if NIRS is used to supplement standard fish ageing methods. However, there is considerable time (i.e., at least 3 years) and start-up costs to develop and validate NIRS calibration models for fish age to a point where only model maintenance is required (i.e., running costs). Results also indicate that NIRS may be particularly useful for spatial (e.g. stock) discrimination. The potential applicability of NIRS was recognised by end-user
stakeholders in Queensland and the Northern Territory, who are proposing further research work.

Understanding what NIRS measures in fish otoliths and how this is correlated with age (or geographic location) was a common desire of fisheries end-users in all jurisdictions, because this knowledge could reduce error and would significantly enhance the applicability of NIRS technology in fisheries science.

Seafood CRC: controlling biofouling of pond aerators on marine prawn farms

Project number: 2011-734
Project Status:
Completed
Budget expenditure: $42,600.00
Principal Investigator: David Mann
Organisation: Department of Primary Industries (QLD)
Project start/end date: 23 Oct 2011 - 12 Oct 2013
Contact:
FRDC
SPECIES

Need

Biofouling of aeration equipment is a significant farm management issue and production cost for Australian marine prawn farms. Defouling aeration equipment has a high labour demand and once fouled the energy efficiency of paddle-wheels and other aerating equipment can be markedly reduced, leading to elevated electricity costs and shorter equipment life. The estimated cost of biofouling is a minimum of $1,000 per hectare per crop when considering the additional labour, maintenance and electricity costs that it creates. This cost figure however does not include the impact of aerator fouling on prawn production levels which potentially could be far greater. The industry uses up to 10x aerators per hectare and they consume 70-80% of total farm energy use. It is estimated that at the peak of the production season around 6,000 2hp aerators are in use in ponds across the prawn industry alone.

Ensuring appropriate and timely aerator defouling is conducted is a significant farm issue. Manual defouling is one of the least desired tasks on the farms as it is dirty, laborious and workers are susceptible to multiple skin cuts that are prone to infection. Consequently it can be difficult to maintain staff to undertake this task for any length of time.
There is no data available on the impact of biofouling on the aeration efficiency, for example the oxygen transfer rate, and this information is critical to maximising benefit from mitigation strategies from both a practical and economic stand point.

The relevant industry body, the APFA through the R&D Committee, has assessed prevention of aerator biofouling as a priority issue and has recommended that the project commence as close as possible to the start of the current production season.

The proposed project falls within the Seafood CRC Theme – ‘Aquaculture Innovation’ as the objective is to improve production efficiency.

Objectives

1. Review biofouling control options and select those with greatest potential for application on prawn farms.
2. Assess the impact of aerator biofouling on prawn farms.
3. Transfer methods for implementing aerator biofouling controls to the prawn farming industry.
4. Evaluate selected biofouling control options under commercial conditions.

Final report

ISBN: 978-0-7345-0438-8
Author: David Mann
Final Report • 2013-11-01 • 1,006.29 KB
2011-734-DLD.pdf

Summary

Biofouling of aeration equipment is a significant farm management issue and production cost for Australian marine prawn farms. Defouling aeration equipment has a high labour demand and once fouled, the energy efficiency of paddle-wheels and other aerating equipment can be markedly reduced, leading to elevated electricity costs and shorter equipment life. The estimated cost of biofouling is a minimum of $1,000 per hectare per crop when considering the additional labour, maintenance and electricity costs that it creates.

The project was designed to assist the Australian prawn farming industry improve aeration efficiency through providing farms with new information pertinent to cost-efficient management of their aerator fleet. The project focussed on the impact of biofouling on aeration and measures to control its accumulation, though broader aspects of aeration were also considered.

On-farm monitoring of aerators determined a huge variation in aerator electrical performance within and among farms. Around 60% of the electrical use variability among paddlewheels is due to biofouling accumulation. This also means that around 40% of differences among paddlewheels is attributable to mechanical factors such as degree of wear and tear.

The overarching output from the research conducted under this project is an improved understanding and quantification of the problem of biofouling in the use of aerators in marine prawn ponds. Farms are under pressure to become increasingly efficient production systems and the information arising from this project will substantially contribute to the farm knowledge base drawn on to optimise strategies that reduce aerator fleet management costs.

Tactical Research Fund: Reducing the impact of discarded recreational fishing tackle on coastal seabirds

Project number: 2011-057
Project Status:
Completed
Budget expenditure: $40,000.00
Principal Investigator: Matthew J. Campbell
Organisation: Department of Primary Industries (QLD)
Project start/end date: 15 Jan 2012 - 15 Mar 2013
Contact:
FRDC

Need

This proposal will address the FRDC Recfishing Research priority area "Assessing the impact of recreational fishing methods on conservation values of aquatic habitats" as announced in the 2012 Annual Competitive Round Call for Expressions Of Interest. After making inquiries with seabird rescue organisations, it has become apparent that seabird entanglements in discarded fishing tackle is of major concern. Australia Zoo Wildlife Hospital report that their rescue unit responded to approximately 300 calls for bird entanglements in discarded fishing tackle in 2011 (to August 1). Further, the Rescue Hotline of Pelican and Seabird Rescue Inc. have received approximately 400 calls in 2011 (to the time of writing - 10/9/2011). These figures are unacceptable and measures need to be taken to decrease the incidence of seabird interactions with discarded recreational fishing tackle. The injuries that result from these interactions are often catastrophic, with a high proportion of entangled animals being euthanised. Healthy Waterways has produced a provocative poster, attached, depicting a pair of budgerigars surrounded by fishing line and hooks. Such negative stereotypes need to be addressed and, as such, there is a need to educate recreational fishers as to the consequences of the careless discarding of fishing tackle. This subject was recently highlighted in a Queensland recreational fishing publication, Queensland Fishing Monthly (see attachment). Both recreational fishing representative groups, Sunfish and Ecofishers, have expressed concern regarding this issue. A fifteen minute search at a secluded recreational fishing location in the northern suburbs of Brisbane by two people resulted in the collection of the fishing tackle pictured in the attachment (13/09/2011 - Tingalpa Creek). Although this could be construed as an environmental problem and beyond the purview of FRDC, it certainly reflects poorly on recreational fishers and, as such, an extension/educational project would help alleviate the problems highlighted in this proposal.

Objectives

1. Quantify the extent of seabird entanglement in northern Moreton Bay
2. Convene a workshop involving relevant stakeholders in order to establish methods for mitigating seabird entanglements
3. Promote the responsible removal of discarded fishing tackle from popular fishing locations in northern Moreton Bay

Final report

ISBN: 978-0-7345-0437-1
Author: Matthew Campbell

Seafood CRC: loss minimisation in farmed prawns through improvements in storage life and colour

Project number: 2010-707
Project Status:
Completed
Budget expenditure: $185,763.69
Principal Investigator: Sue Poole
Organisation: Department of Primary Industries (QLD)
Project start/end date: 30 Apr 2010 - 28 Feb 2013
Contact:
FRDC

Need

The prawn industry is being challenged to provide prawn product that maintains premium quality throughout an extended storage-life. Significant revenue loss for farmed prawns within the retail sector occurs through product not meeting market colour specification and also severe price reduction near the end of chilled storage life. The losses can be minimised by optimising on-farm production protocols to maintain a consistent colour in farmed prawns during frozen storage and by gaining additional shelf-life of chilled cooked prawns throughout the supply chain.

The opportunity is to introduce a range of technologies, along with widely-adopted proven best practise, that maintains premium quality after cooking and freezing. The technologies will incorporate natural compounds with antimicrobial/antioxidant properties, synergistic packaging, glazing and thereby extend chilled storage-life of the prawn whilst maintaining optimal quality. By maximising astaxanthin level at harvest and reducing degradation during storage, consistent premium red-orange colour of prawns will be assured for the retail market.

This research addresses the Program 3: Improving Farmed Prawn Market Value priority of APFA’s Five Year R&D Plan for the Australian Prawn Farming Industry, 2007-2012. Program 3 seeks to achieve the objective of increasing the average price received for Australian farmed prawns to $16.50/kg.

Objectives

1. Evaluate the effectiveness of natural extracts as preservation technologies for reducing microbial loads
2. Compare various packaging options which are available commercially and select the most appropriate for synergistic extension storage life and to develop ready-to-go concept products
3. Develop optimised on-farm processing protocols by combining the most suitable natural compounds and packaging to obtain premium quality prawns
4. Summarise current available information on world-wide best practice operations that maximise red colour in cooked prawns
5. Determine rate of astaxanthin loss during processing and frozen storage
6. Establish required astaxanthin level in harvested prawns to retain colour level (APFA 8+) through subsequent long-term frozen storage
7. Identify synergistic benefits of a combination of techniques, including live harvest holding (black tank) and optimal glazing methods on astaxanthin levels and colour retention
8. Extend and illustrate optimal protocols to the industry partners through on-farm trials

Tactical Research Fund: incidence and possible causes of saddleback syndrome in the fish species of south east Queensland

Project number: 2010-070
Project Status:
Completed
Budget expenditure: $40,414.00
Principal Investigator: Matthew J. Campbell
Organisation: Department of Primary Industries (QLD)
Project start/end date: 30 Jun 2011 - 29 Jul 2012
Contact:
FRDC

Need

The yellowfin bream is an important commercial and recreational species in Queensland, with over 400t landed annually. Anecdotal evidence suggests that the incidence of Saddleback Syndrome (SBS), a skeletal deformity primarily affecting the development of the dorsal fin spines, is increasing. This increase in the prevalence of SBS may result in recreational fishers and the public losing confidence in catching and consuming fish afflicted with the syndrome. It is, therefore, important that objective information is available to counter these concerns.

This project addresses one of the Queensland Fisheries Research Advisory Board's Research & Development priorities for 2010. Specifically, it addresses the "urgent need for research into the cause of a particular deformity of bream that is occurring in the important Moreton Bay fishery". The Queensland Fisheries Research Advisory Board (QFRAB) recently advised that a desktop study should be undertaken to determine potential causes of SBS in the international literature. Additonally, QFRAB advised that data from DEEDI's Long Term Monitoring Program should be analysed to determine the extent of SBS in Queensland.

Mr. Tony Ham, Fisheries Queensland Manager of Recreational Fisheries, has advised that SBS is an important issue, requiring attention in order to determine the incidence and cause of the syndrome in key recreational fish species.

Further, the need to investigate the cause of saddle-back deformities in yellowfin bream in SE Qld is of major importance and concern to the recreational fishing sector according to Sunfish Queensland, the recreational fishing representative group on the inshore finfish Scientific Advisory Group (SAG).

This project also addresses the concerns of commercial net fishers in Moreton Bay, specifically through the Moreton Bay Seafood Industry Association. These concerns relate to the potential difficulties in marketing bream and other species suffering from Saddleback Syndrome considering the perceived increase in the syndrome’s prevalence in recent years.

Objectives

1. Quantify the extent of Saddleback Syndrome in Queensland using existing databases
2. Review National and International published and grey literature in order to document the occurrence of Saddleback Syndrome and its causes
3. Convene a workshop of stakeholders to present the results of the database searches and literature review

Final report

ISBN: 978-0-7345-0434-0
Author: Matthew Campbell

Tactical Research Fund: assessing technology changes and risks to the sustainable management of deepwater line fisheries in southern Queensland

Project number: 2010-053
Project Status:
Completed
Budget expenditure: $45,000.00
Principal Investigator: Wayne Sumpton
Organisation: Department of Primary Industries (QLD)
Project start/end date: 31 Dec 2010 - 30 Jan 2012
Contact:
FRDC

Need

There is a risk that Queensland’s deepwater (>200m) fish stocks are being fished at levels that are not sustainable Increased effort by commercial fishers in deep waters under normal line entitlements and the lack of knowledge of many of the targeted deepwater species (many of these species are presumed to be long-lived, schooling species susceptible to overexploitation) are key threats.

There is also anecdotal evidence that recreational fishers are fishing further offshore in deeper water and that recent improved fishing technologies have increased the effective effort on deepwater fish by all sectors. It is imperative that basic information on these deep-water stocks is collected at a time when management arrangements are being updated for rocky reef species (both shallow and deepwater).

The DEEDI Fisheries Observer Program has 100 days allocated to the deepwater fishery next calendar year, providing a unique opportunity to gather additional biological information on deepwater species that would not normally be collected as part of the routine core functions of the program. The provision of resources via a short-term “Tactical Research Fund” proposal would provide additional information at a time that is critical in the management cycle. It would also enable an assessment of the impact of improvements in vessel and gear technology (braided line, soft plastic lures, GPS etc) that could be used to better standardise catch rates. This is an essential requirement in any future stock assessments.

Fisheries Queensland is currently undertaking a gap analysis to identify information needs for completing an ecological risk assessment of this fishery. The proposed research is critical as it is designed to address the data deficiencies identified by that analysis.

Fisheries Queensland has identified this issue as a priority to be addressed over the next 18 months with a view to review management arrangements in 2012.

Objectives

1. Quantify recent changes in fishing technology in the commercial, recreational and charter line fishing sectors and evaluate the extent to which these changes could impact on the sustainability of deepwater fish species.
2. Collect and analyse biological data on key deep-water line caught fish species in collaboration with the fisheries observer program and collate information identified by the gap analysis currently being undertaken by Fisheries Queensland.
3. Assist Fisheries Queensland in developing an ecological risk assessment for deepwater fin fish species.

Aquatic Animal Health Subprogram: Investigation of an emerging bacterial disease in wild Queensland gropers, marine fish and stingrays with production of diagnostic tools to reduce the spread of disease to other states of Australia

Project number: 2010-034
Project Status:
Completed
Budget expenditure: $220,640.21
Organisation: Department of Primary Industries (QLD)
Project start/end date: 31 Aug 2010 - 29 Aug 2013
Contact:
FRDC
SPECIES

Need

Streptococcus agalactiae is an emerging disease in Australian fish. A challenge model in Qld groper is required to understand the pathogenesis and mode of transmission of S. agalactiae. This information is critical to determine how to manage the spread of disease and identify likely sources of infection. Information generated from this trial will be useful for finfish farmers and marine aquaria to minimise chances of infection, and for state government veterinarians to develop future biosecurity plans for control and eradication of the disease.

There are no diagnostic tests available in Australia to detect S. agalactiae in fish other than bacteriology that takes up to a week for results. Development of rapid and accurate diagnostic tools including Gold standard PCR and in situ hybridisation for S. agalactiae are urgently needed by State and private Veterinary Laboratories to enable rapid diagnosis of S. agalactiae in marine fish and elasmobranchs. Availability of these tools will have flow-on benefits for all States of Australia, allowing increased aquatic animal disease detection and will enhance current and future State surveillance and monitoring of Streptococcosis in fish. Current disease surveillance of fish at aquaculture sites within the GBRMP will be enhanced by the availability of more accurate and rapid diagnostic tools.

Different strains of S. agalactiae have been isolated from Qld gropers, wild marine fish and stingrays in north Queensland by QPIF. There is a need for the development of molecular typing tests to accurately identify different strains of S.agalactiae in order to determine origin (native or exotic) and routes of dissemination of infection, thus enabling AQIS to make informed decisions in relation to the importation of fish. Moreover, commercial vaccine companies can produce effective autogenous vaccines to protect at-risk populations of fish and elasmobranchs, benefiting commercial aquariums, marine aquaculture and research facilities by protecting valuable stock.

Objectives

1. Perform a challenge infectivity trial in Qld groper to prove experimentally that S. agalactiae causes mortalites in Qld groper (or barramundi) to fulfil Koch's postulates
2. Develop reliable, rapid and accurate diagnostic tools to enable detection of the bacterial disease streptococcosis caused by Streptococcus agalactiae in marine fish
3. To perform phylogenetic comparison of Australian fish, human and animal strains of S. agalactiae with overseas fish and animal S.agalactiae strains, to determine their genetic relatedness and origin of the groper strain (introduced or endemic)
4. To create a library of different S. agalactiae strains enabling utilisation by scientific researchers

Final report

ISBN: 978-0-7345-0446-3
Final Report • 2015-05-06 • 4.74 MB
2010-034-DLD.pdf

Summary

This project has assisted in ensuring the future sustainability and profitability of the aquaculture industry and natural fisheries resources in Queensland, Australia (including the Great Barrier Reef Marine Park), by providing industry, the public, State and Commonwealth governments with improved understanding of the occurrence of Streptococcus agalactiae in fish and crustaceans in coastal Queensland.

The project demonstrated that S. agalactiae is highly pathogenic to juvenile Queensland grouper, E. lanceolatus and can be spread via infected water, infected food, by injection, or by cohabitation (of S. agalactiae-infected fish with non-infected fish).

This project produced rapid, reliable and accurate diagnostic tools, including PCR and an Immuno-histochemistry (IHC) method to specifically detect S. agalactiae in fish, thus increasing the States’, Territories’, and Australia’s capability for disease testing, surveillance, monitoring and reporting.

Molecular studies showed the S. agalactiae isolates from Queensland grouper were genetically distinct from human, cow, dog, cat or crocodile strains. Molecular studies further showed the Queensland grouper S. agalactiae isolates were most closely related to the grunter, mullet, catfish and stingray strains isolated from north Queensland. All North Queensland fish isolates belonged to strain type ST-261. Importantly, this sequence type has not been associated with human infection anywhere in the world and is substantially different from all terrestrial isolates of GBS.

Knowledge gained from this project will assist in developing biosecurity, health and disease management plans and programs relating to disease control for S. agalactiae in aquaculture facilities, commercial marine aquaria and live reef fish holding facilities. The project has resulted in outcomes that support consumer confidence in the safety of Australian seafood as the S. agalactiae isolates from fish are genetically different from those isolated from mammals and have never caused disease in terrestrial animals to date. Outcomes of this project have assisted in protecting recreational fisheries, through improved knowledge on prevalence and distribution of S. agalactiae within fish species of the Great Barrier Reef Marine Park in Northern Australia.

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