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Industry

Aquatic Animal Health Subprogram: development of a laboratory model for infectious challenge of Pacific oysters (Crassostrea gigas) with ostreid herpesvirus type-1

Project number: 2012-052
Project Status:
Completed
Budget expenditure: $134,990.00
Principal Investigator: Peter D. Kirkland
Organisation: Department of Primary Industries and Regional Development (NSW)
Project start/end date: 19 Dec 2012 - 29 Jun 2014
Contact:
FRDC

Need

The need for further information to assist with the response to OsHV-1 is universally acknowledged by the aquaculture industry broadly, government agencies charged with biosecurity and aquatic animal health scientists in Australia and internationally.
Access to a standardised, reproducible and transferable laboratory infection model is critical to ongoing research efforts. Such a model provides a precise method of testing the effect of factors which are suspected to influence the outcome of an infectious challenge with OsHV-1 on Pacific oysters. The most promising factor for enabling continuation of Pacific oyster production despite the threat of OsHV-1 infection is the identification of genetic variation in susceptibility to POMS. The demand for efficient progress in selective breeding programmes requires a laboratory infection model which is suitable for screening large numbers of candidate families and provides results which can be reliably interpreted.

Objectives

1. Production and long-term storage of large quantities of a standard OsHV-1 inoculum
2. Evaluation of methods to provide a standard, measured OsHV-1 infection challenge
3. Definition of the outcomes of infectious challenge including mortality, development of histopathological lesions and quantities of OsHV-1 present in tissues
4. Determination of the dose-response under standardised challenge conditions, including variation due to the age of the oysters
5. Determination of the repeatability, reproducibility and transferability of the standard infection challenge
6. Assessment of the susceptibility of flat oysters (spat and adult) to OsHV-1 infection.
People
PROJECT NUMBER • 2002-661
PROJECT STATUS:
COMPLETED

Aquatic Animal Health Subprogram: enhancing the emergency disease response capability of NSW and Qld Government agencies and industry bodies associated with oyster culture

AQUAPLAN was generated as a National Strategic Plan for Aquatic Animal Health in recognition of the growing importance of protecting fisheries and aquaculture industries from disease. This project allowed NSW Fisheries to begin implementing one component of the National AQUAPLAN objectives,...
ORGANISATION:
Department of Primary Industries and Regional Development (NSW)
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