17 results
Adoption
PROJECT NUMBER • 2018-127
PROJECT STATUS:
COMPLETED

Validation and implementation of rapid test kits for detection of OsHV-1

Following the outbreak of Pacific Oyster Mortality Syndrome (POMS) in the Port River, South Australia in summer 2017-18, a need was identified for rapid diagnostic technology for OsHV-1, the causative agent of POMS. During the February 2016 OsHV-1 outbreak in Tasmania, tracing activities in...
ORGANISATION:
SARDI Food Safety and Innovation
Environment
PROJECT NUMBER • 2017-051
PROJECT STATUS:
COMPLETED

Seafood CRC: Extending biotoxin capability and research in Australia through development of an experimental biotoxin contamination facility to target industry relevant issues

A short-term experimental biotoxin contamination facility was set up at Roseworthy, South Australia, to examine the uptake and depuration of marine biotoxins from one of the most toxic dinoflagellates known, Alexandrium catenella. Over the period of one year, SARDI’s Seafood Food Safety group...
ORGANISATION:
SARDI Food Safety and Innovation

Future oysters CRC-P: Species diversification to provide alternatives for commercial production

Project number: 2016-807
Project Status:
Completed
Budget expenditure: $243,426.00
Principal Investigator: Xiaoxu Li
Organisation: SARDI Food Safety and Innovation
Project start/end date: 23 Apr 2017 - 29 Jun 2019
Contact:
FRDC

Need

The establishment of a new native oyster and/or western rock oyster aquaculture sectors in SA and the former in Tasmania will not only diversify the business risk of the existing Pacific oyster sector, but has the potential to become a multi-million dollar industry itself. As native oysters would be more suited to subtidal or low intertidal culture while western rock oysters are an ideal alternative species to mitigate POMS, the successful development of these aquaculture sectors will strengthen the confidence of existing/new growers and investors in Pacific, western rock and native oysters; thereby encouraging further expansion of the industry. In addition, supporting species diversification is one of the high strategic priorities in the Oysters Australia Strategic Plan 2014-2019.

Objectives

1. To develop Native Oyster on-farm growing methods that maximise survival and growth in South Australia and Tasmania
2. To compare the performance between Pacific Oysters and Native Oysters in South Australia
3. To establish a Native Oyster farmers network to share new techniques and knowledge
4. To develop translocation protocols for the safe translocation of Western Rock Oysters to South Australia
5. Trial Western Rock Oysters in the field in South Australia to assess their performance and viability of a potential industry if agreed by industry and regulators

Final report

ISBN: 978-1-8767007-45-4
Authors: Xiaoxu Li Penny Miller-Ezzy Christine Crawford Deborah Gardner Marty Deveney Jessica Buss Ben Diggles Kathryn Wiltshire
Final Report • 2023-05-01 • 3.61 MB
2016-807-DLD.pdf

Summary

Pacific Oyster Mortality Syndrome (POMS), the disease caused by OsHV-1 microvariant, results in high and rapid mortality in Pacific Oysters (Crassostrea gigas) and has been responsible for significant economic loss to oyster industries in Australia and around the world. The diversification of commercial production into different oyster species (Native Oysters and Rock Oysters), that are not susceptible to POMS, has been proposed as a way to mitigate the risk of POMS in southern Australia. However, the Australia Native Oyster (Ostrea angasi) industry is still in its infancy, with knowledge gaps along the production chain. Additionally, there are no wild populations of Rock Oysters (Saccostrea sp.) in South Australia. Despite Rock Oyster aquaculture being well established in New South Wales and recently in Western Australia they have never been commercially produced in South er Australia and translocation policies to move them around the state are non-existent. This project aimed to improve on-farm production of Native Oysters and determine if Rock Oysters can be safely translocated to South Australia from Western Australia, to help Australian oyster growers to diversify into these species.

Future Oysters CRC-P: Advanced aquatic disease surveillance for known and undefined oyster pathogens

Project number: 2016-806
Project Status:
Completed
Budget expenditure: $829,300.00
Principal Investigator: Marty R. Deveney
Organisation: SARDI Food Safety and Innovation
Project start/end date: 17 Apr 2017 - 29 Jul 2019
Contact:
FRDC

Need

During the February 2016 OsHV-1 outbreak in Tasmania, tracing activities in Tasmania and South Australia required substantial follow-up and surveillance to define diseased areas and prove that response measures had prevented entry of OsHV-1. This surveillance was expensive, and PIRSA and DPIPWE developed a strategy to decrease cost for future surveillance. Cost estimates for ongoing surveillance for early detection have been prohibitive, and both the Australian Pacific oyster aquaculture industries and State governments have expressed a need for more cost effective surveillance options for monitoring disease in affected areas and early detection in currently unaffected regions.

Winter mortality is a major cost impost on the Sydney Rock Oyster industry. Its current status as a syndrome of unknown cause prevents methods from being developed to minimise losses, and an improved understanding of its cause is required to begin to develop management strategies. Mitigating losses will increase profitability for the Sydney rock oyster industry.

SA oyster mortality syndrome (SAMS) is a sporadic, regionally concentrated occurrence of high mortality that is not associated with readily detectable pathogens. The use of the terms SAMS implies that these mortalities have commonalities but this is not proven. This project will aim to provide a focused approach to developing a case definition for SAMS and as a result help direct mitigation strategies to reduce or remove the problem. If a cause can be isolated, an on farm decision tool swill be developed to allow better ‘trigger point’ identification for when farm managers need to engage diagnosticians or instigate identified mitigations strategies.

Objectives

1. Winter mortality: causative agent investigation, case definition, management strategies, improved husbandry and validate WM resistance assessments for Sydney rock oysters
2. SA mortality syndrome: causative agent investigation, improved understanding of causes, case definition, improved diagnostic technologies and improved husbandry to maximise survival
3. POMS: improved surveillance methods for early detection, to manage spread and understand transmission, novel detection and enumeration method based on flow cytometry

Final report

ISBN: 978-1-876007-24-9
Author: Marty R Deveney & Kathryn H Wiltshire (Editors)
Final Report • 2020-03-01 • 12.34 MB
2016-806 DLD.pdf

Summary

This project improved understanding of methods for surveillance for several diseases of farmed oysters. Surveillance is a critical component of biosecurity and aquatic animal health activities.  Surveillance supports understanding health status of populations of animals, provides evidence to support claims of freedom or understanding prevalence and increases the likelihood that a new or emergent disease can be controlled.

Mollusc diseases are less well understood than terrestrial animal and many finfish diseases and this project sought to develop understanding of three oyster diseases of substantial economic impact in the Australilan edible oyster aquaculture industries:

Pacific Oyster mortality syndrome (POMS), South Australian mortality syndrome (SAMS), and winter mortality (WM). 

 
The project included activities with four main aims:
 - Improving understanding of tests for OsHV-1 and investigation of using these tests for area surveillance.
 - Development of a low-cost, rapid test for OsHV-1.
 - Refinement of the case definition and investigation of the cause of SAMS in Pacific Oysters.
 - Development of a case definition and improving understanding of the cause of Winter Mortality in Sydney Rock Oysters. 

eSAMarine – phase 1: the first step towards an operational now-cast/forecast ocean prediction system for Southern Australia

Project number: 2016-005
Project Status:
Completed
Budget expenditure: $86,379.00
Principal Investigator: John Middleton
Organisation: SARDI Food Safety and Innovation
Project start/end date: 30 Jun 2016 - 14 Aug 2017
Contact:
FRDC

Need

Commercial in confidence. To know more about this project please contact FRDC.

Objectives

Commercial in confidence

Oysters Australia IPA -workshop – identifying knowledge gaps for development of the native oyster aquaculture industry in South Australia

Project number: 2015-229
Project Status:
Completed
Budget expenditure: $19,074.00
Principal Investigator: Xiaoxu Li
Organisation: SARDI Food Safety and Innovation
Project start/end date: 31 Dec 2015 - 29 Jun 2016
Contact:
FRDC

Need

The establishment of a new native oyster aquaculture sector in SA will not only diversify the business risk of the existing Pacific oyster sector, but has the potential to become a multi-million dollar industry itself. As native oyster is an ideal alternative species to mitigate POMS, the successful development of this aquaculture sector will strengthen the confidence of existing/new growers and investors in both Pacific and native oysters; thereby encouraging further expansion of the industry. In addition, supporting species diversification is one of the high strategic priorities in the Oysters Australia Strategic Plan 2014-2019.

Objectives

1. To identify knowledge gaps for development of the native oyster aquaculture industry in Australia
2. To provide advice on the key research and development requirements to support the native oyster aquaculture development in South Australia

Final report

ISBN: 978-1-921563-95-9
Author: Xiaoxu Li and Penny Miller-Ezzy
Final Report • 2017-05-01 • 6.68 MB
2015-229-DLD.pdf

Summary

The 2015-229 “Oysters Australia IPA - workshop - identifying knowledge gaps for development of the native oyster aquaculture industry in South Australia” brought together oyster farmers, hatchery operators and scientists from across Australia to share their knowledge and experience with native oyster (Ostrea angasi) aquaculture and help to identify the key knowledge gaps in the production chain. Through presentations and group discussions, a number of research and development needs were identified. These were categorised into seven key areas: early life history/genetic improvement, farming, oyster health, post-harvest, marketing and branding, industry network and training and education. Across these categories, 19 key research and development needs were identified and prioritised via a post-workshop survey. The following four research and development needs were prioritised as being most important

  • Having a constant and reliable spat supply.
  • Development of a selective breeding program to improve Bonamia resistance, growth rate, meat/shell ratio, colour, lustre, etc.

  • Establishment of good husbandry practices (e.g. handling, density, growing heights, sub-tidal vs. intertidal, seasonal effects, a system to suit O. angasi production).

  • Increase the shelf life/improve packaging/develop processing methods.

 Identifying these needs will help to target future research to meet industry priorities and establish/develop the South Australian/Australian native oyster industry.

 Keywords: Native oysters; Ostrea angasi; Australia; aquaculture

Assessing occurrence of pathogenic species of the marine bacteria Vibrio in Tasmanian oysters from St Helens

Project number: 2015-042
Project Status:
Completed
Budget expenditure: $29,520.38
Principal Investigator: Tom Madigan
Organisation: SARDI Food Safety and Innovation
Project start/end date: 29 Feb 2016 - 29 Jun 2016
Contact:
FRDC

Need

This is the first time that an illness associated with Vibrio has been traced-back to Tasmanian oysters. Regrettably, this incident occurred in the only major harvesting area in Tasmania that has not been impacted by the current Pacific oyster mortality event.

In Australia the control of Vibrio is currently limited to temperature controls during storage or transport. Pre-harvest controls used by the shellfish quality assurance programs are predicated on controlling risk posed by faecal contamination and biotoxins and are not suitable for controlling risk from these naturally occurring bacteria. Although the recent implementation of the Codex Standard for pathogenic marine vibrios suggests risk in bivalve growing areas should be assessed to ascertain the risk to public health, there has been limited research undertaken in Australia. The studies undertaken to date have generally been short in nature with no comprehensive longitudinal studies being undertaken and methodologies have now progressed significantly, whereas New Zealand has been undertaking a long-term survey to understand the risk posed by these pathogens (Cruz, Hedderley & Fletcher 2015). This issue may become a risk in accessing key markets that are active in monitoring or who regulate for these pathogens.

There is an immediate need to collect information on prevalence for the remainder of the summer period to understand the risk and evaluate if there is a relationship to salinity, temperature and toxic strains. This information will be immediately useful for developing appropriate management plans in this growing region.

This illness outbreak will likely result in Tasmanian Shellfish Quality Assurance Program and the other state programs having to consider how to manage risk in the growing areas and establish what is an acceptable level. The work proposed here could be used as a framework for future work that assesses risk across the bivalve industry Australia-wide.

Objectives

1. Assess for the prevalence of pathogenic Vibrio species in the St Helens harvesting region
2. Assess for the presence of genes associated with virulence in Vibrio parahaemolyticus
3. Evaluate if a relationship exisits that between prevalance and sea water temperature and salinity

Final report

ISBN: 978-1-921563-92-8
Authors: Tom Madigan Kate Wilson Gayle Smith and Alison Turnbull

Oysters Australia IPA: the use of FRNA bacteriophages for rapid re-opening of growing areas after sewage spills

Project number: 2015-037
Project Status:
Completed
Budget expenditure: $270,273.00
Principal Investigator: Kate Hodgson
Organisation: SARDI Food Safety and Innovation
Project start/end date: 29 Feb 2016 - 30 Mar 2018
Contact:
FRDC

Need

Oyster growing areas with reticulated sewage and/or waste water treatment plants (WWTP) in the catchment are all potentially affected by closures caused by sewage spills and overflows. Standard risk management practice following such events is to instigate a 21 day closure resulting in lost production, market share, reputational damage, and potential recall costs. The number of growing area closures related to sewage spills and overflows in Australia is significant. In NSW, harvest areas were closed on 100 occasions due to sewage spills between July 2009 and June 2014, resulting in 2688 days of lost sales. The use of FRNA phage as potential indicators of human enteric viruses could lead to a 50% reduction in the number of days closed.
In Tas, there have been 75 harvest area closures of greater than 21 days relating to sewage spills over the past 5 years, resulting in at least 1575 days of lost sales. One three week closure in the Pittwater growing area is estimated to cost approx. $250-$400k combined sales, depending on the season. The cumulative impact of these spills has been estimated to reduce the value of businesses by a combined value of $12 million. Not all sewage incidents result in human enteric viral contamination of shellfish. Factors such as the level of illness in the community, treatment level of waste, size of the spill, hydrodynamics of the growing area, and local growing area conditions all influence the whether a spill results in significant contamination of oysters. FRNA phages have not been used routinely as indicators in shellfish in Australia to date. If FRNA phage levels show contamination is negligible, regulators may allow re-opening of growing areas as early as 10 days after the spill following results from testing on day 7, significantly decreasing the cost of spills to growers. FRNA phages are also showing potential as general indicators of human pathogenic risk.

Objectives

1. Establish baseline levels of FRNA bacteriophages in “at risk” Australian growing areas
2. Determine appropriate sampling plans for FRNA bacteriophages in shellfish following sewage incidents
3. Enable implementation of FRNA phage levels as a management tool for use following adverse sewage incidents in bivalve shellfish growing waters
4. Train laboratories to be competent in using appropriate testing methodologies for FRNA phages in shellfish

Report

ISBN: 978-1-876007-08-9
Authors: Kate Hodgson Valeria Torok Jessica Jolley Navreet Malhi Alison Turnbull
Report • 2018-09-01 • 4.55 MB
2015-037-DLD.pdf

Summary

The South Australian Research and Development Institute (SARDI) Food Safety and Innovation (FSI) group with the support of the Fisheries Research and Development Corporation (FRDC), TasWater, Central Coast Council, Port Macquarie-Hastings Council, Shoalhaven Council, New South Wales Food Authority (NSWFA), New South Wales Shellfish Program, Oysters Australia and Tasmanian Shellfish Quality Assurance Program (TSQAP) undertook an investigation of the use of FRNA bacteriophages (phages) as indicators of viral contamination in shellfish after adverse sewage events. The human enteric viruses Norovirus (NoV) and Hepatitis A virus (HAV) are the most commonly reported foodborne viral pathogens associated with shellfish. The viruses are bioaccumulated by shellfish when sewage enters water in the growing areas. Oysters were sampled from ‘at-risk’ growing areas to establish background baseline phage levels from July 2016 to December 2017. Five adverse sewage events were also investigated during the same period. In addition, training in the laboratory techniques for phage enumeration was undertaken at the SARDI FSI laboratory in Adelaide for staff from laboratories in NSW and Tasmania.

Survey of Foodborne Viruses in Australian Oysters

Project number: 2013-234
Project Status:
Completed
Budget expenditure: $260,800.00
Principal Investigator: Valeria Torok
Organisation: SARDI Food Safety and Innovation
Project start/end date: 23 Feb 2014 - 22 Oct 2015
Contact:
FRDC

Need

Norovirus (NoV) is the leading cause of gastroenteritis outbreaks worldwide, and is commonly associated with shellfish. Between 2001-2010 seventeen Australian cases of suspected shellfish related NoV outbreaks were reported in OzFoodNet (Knope, 2011). More recently (March 2013) 400 people were reportedly affected by NoV following consumption of contaminated oysters from Tasmania.
Virus contamination in food poses major concerns for consumers and can impact export trade. The WHO/FAO working group (2008) on food-borne viruses, and European Food Safety Authority’s (EFSA) opinion (2011) ranked bivalves among the highest risk food groups in terms of NoV and hepatitis A virus (HAV). In 2011 the Codex Committee on Food Hygiene ratified a draft guideline on viruses in foods, with a specific annex on bivalves. It recommends that countries monitor for NoV and HAV in bivalves following high risk pollution events. Additionally, in early 2012 EFSA recommended the introduction of an acceptable NoV limit in oysters and the EU Community Reference Laboratory (2012) recommended an ‘absence’ criterion be applied for HAV in bivalves. Because of these impending international regulations (noting that some importing nations already require NoV testing), the Australian oyster industry members have indicated that they would like a more comprehensive evaluation of the prevalence of viruses in Australian oysters. There is little information on the baseline levels of NoV in Australian oysters. Although, a small pilot survey in oysters was conducted in production areas, more information is needed.
Recent developments made at SARDI in the use of molecular biology techniques for virus detection in foods would enable the occurrence of these viruses in bivalves to be determined through a virus prevalence survey. Similar surveys have been undertaken worldwide, including in the USA, UK, France and China, and might contribute to the development of market access strategies at the international level.

Objectives

1. 1 To design a statistically robust survey to evaluate virus occurrence in oyster growing areas in NSW, Qld, SA and Tas
2. 2 To identify the prevalence of NoV and HAV associated with Australian oysters at harvest
3. 3 To use the survey results to support trade and market access of Australian oysters

Seafood CRC: overseas market access for shellfish

Project number: 2009-752
Project Status:
Completed
Budget expenditure: $5,000.98
Principal Investigator: Catherine McLeod
Organisation: SARDI Food Safety and Innovation
Project start/end date: 31 Aug 2009 - 30 Nov 2009
Contact:
FRDC

Need

The potential reduction of regulatory thresholds for marine biotoxins in the EU will have significant negative economic consequences for the Australian oyster, mussel, scallop and abalone industries (and pipi’s if domestic regulatory thresholds also changed).

The scallop, oyster and mussel industry currently export product to the EU. Total scallop exports to the EU in 2006/2007 were valued at around $4,551,000 AUD (187 t). Other mollusc (including oysters and mussels) exports to the EU in 2006/2007 were around $1,084,000 AUD (255 t). Due to the periodic occurrence of okadaic acid toxins and saxitoxins in Australian shellfish the implementation of reduced regulatory levels would reduce the amount of product eligible for export to the EU.

Exports of Australian abalone to the EU ceased in 2007, this was in part due to the enforcement of marine biotoxin regulatory limits by the EC. The wild caught abalone industry is attempting to regain market access to the EU through determining alternate risk management procedures for marine biotoxins in abalone. The reduction of regulatory levels for marine biotoxins may impinge on future EU access arrangements for Australian abalone.

Experience has repeatedly demonstrated that European decisions can impact on other more commercially significant markets, including Asia and the domestic market. Codex may also be prompted to change marine biotoxin guidance levels in response to EU changes. Wide spread adoption of reduced regulatory levels for marine biotoxins would result in increased growing area closures in Australia and less product eligible for sale. This proposal aims to assist in maintaining the current EU regulatory limits for marine biotoxins which will allow the current amount of shellfish to be exported to the EU and avoid other markets being influenced.

Objectives

1. Undertake robust technical review of the European Food Safety Authority risk assessments on saxitoxin and okadaic acid group toxins.
2. Submit the technical review and a rationale for maintaining current marine biotoxin regulatory limits to the European Commission.
3. Convene a working group to determine future steps required to mitigate potential lowering of marine biotoxin regulatory limits.

Final report

ISBN: 978-0-9756044-8-9
Authors: Dr Catherine McLeod Dr John Sumner Dr Andreas Kiermeier
Final Report • 2011-02-24 • 1.18 MB
2009-752-DLD.pdf

Summary

The oyster, scallop and mussel industries currently export product to the EU. Due to the periodic occurrence of Okadaic Acid (OA) and Saxitoxin (STX) group toxins in Australian shellfish the implementation of reduced regulatory levels would reduce the amount of product eligible for EU export. Exports of Australian abalone to the EU ceased in 2007, due in part to the enforcement of marine biotoxin regulatory limits set by the EC. The wild caught abalone industry is attempting to regain EU market access through determining alternate risk management procedures for marine biotoxins in abalone.

The European Commission (EC) requested the European Food Safety Authority (EFSA) to assess the current European Union (EU) limits for shellfish regarding human health and methods of analysis for various marine biotoxins, including newly emerging toxins. A critical recommendation of the ‘EFSA Opinions’ is that the regulatory limits should be significantly lower (more stringent) for both OA and STX.

The objectives of this study were to:

  1. Undertake a robust technical review of the EFSA risk assessments on STX and OA group toxins.
  2. Submit the technical review and a rationale for maintaining current marine biotoxin regulatory limits to the EC.
  3. Convene a working group to determine future steps required to mitigate potential lowering of marine biotoxin regulatory limits.
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