49 results

Seafood CRC: Towards all female Penaeus monodon populations using androgenic gland manipulations

Project number: 2009-759
Project Status:
Completed
Budget expenditure: $30,033.00
Principal Investigator: Abigail Elizur
Organisation: University of the Sunshine Coast (USC)
Project start/end date: 19 Mar 2010 - 29 Jul 2012
Contact:
FRDC

Need

The Australian prawn farmers are under intense competition from cheaper imported prawns. Most of the imported prawns are in the small size segment. Female prawns grow larger then males, therefore the creation of all-female prawn populations will provide a much needed competitive advantage and enable a more profitable farming of P. monodon in Australia.

Objectives

1. Optimise procedure for neo-male creation using androgenic gland implantations and MT treatments.
2. Cross neomales with females to establish viability and fertility of WW prawns
3. Grow WW P. monodon females on farm and research provider facilities to reach sexual maturity.
4. To cross WW P. monodon females with wild males (both on farm and at the research provider facility) and obtain viable all female offspring.
5. To assess the reproductive performance of WW females compared to normal WZ females
6. Assess production performance of all-female offspring compared with mixed population (on farm).

Final report

ISBN: 978-1-925983-38-8
Author: Abigail Elizur
Final Report • 2012-07-30 • 1.24 MB
2009-759-DLD.pdf

Summary

Australian prawn aquaculture production is based predominantly on P. monodon farming, with larger sized prawns attract premium prices in the local market. Females grow significantly larger than males, and hence, a technology to develop all-female monosex populations would offer competitive advantage at a local as well as international market. The primary objectives of this study were to first produce, and then cross, WW P. monodon females and obtain viable all-female offspring.

Unfortunately, the trials to achieve neo-males resulted only in external signs of masculinisation. Hence, other objectives could not be addressed. Since the project did not meet the agreed go-no go point, it was prematurely terminated. However, suggestions for subsequent studies are given to forward this research into the future.

SCRC: PhD Sustainable Aquaculture Development Through Effective Policies.(Mark Oliver)

Project number: 2009-725
Project Status:
Completed
Budget expenditure: $0.00
Principal Investigator: Mark Oliver
Organisation: University of the Sunshine Coast (USC)
Project start/end date: 16 Aug 2009 - 16 Aug 2013
Contact:
FRDC

Need

The role of creating an environment for sustainable aquaculture development throughout Australia is critical in reaching the industries primary goal of doubling the value of the Australian seafood industry to $4 billion by 2017 and generating more than 2,800 new jobs in rural and regional areas. Enhancing a regions capacity to undertake sustainable marine aquaculture development from an initial policy driven perspective brings with it a heightened level of investor confidence and regional based skills development and acquisition.

A recent report undertaken by the Australian Productivity Commission (2004) found amongst others that:

• Aquaculture production is subject to an unnecessarily complex array of legislation and agencies.
• State aquaculture and/or fisheries legislation have multiple objectives and these are not always clearly defined.
• State government departments primarily responsible for aquaculture regulatory arrangements often have potentially conflicting
of policy development, implementation of regulation, industry promotion and development, and research.
• New South Wales, Queensland and Western Australia have made limited progress with marine aquaculture planning. This may constrain marine aquaculture, or result in ad hoc approvals for individual sites, and conflicts over resource use.

The Queensland Department of Primary Industries and Fisheries have identified a number of areas that have to be addressed in order to develop a sustainable aquaculture industry in Queensland, two of these are Policy and Legislation as well as Aquaculture planning;

There is also an identified gap in aquaculture planning within the Great Barrier Reef Marine Park Authority. The Great Barrier Reef Marine Park Authorities own Position Statement on Aquaculture Development within the Great Barrier Reef Marine Park (2004) states that ‘Guidelines for the assessment of applications to conduct intensive aquaculture in the GBRMPA need to be developed’.

Seafood CRC: genetic technologies to support a transformation to profitability and competitiveness in F. merguiensis and P. monodon

Project number: 2009-724
Project Status:
Completed
Budget expenditure: $265,540.00
Principal Investigator: Wayne Knibb
Organisation: University of the Sunshine Coast (USC)
Project start/end date: 14 Dec 2009 - 30 Mar 2013
Contact:
FRDC

Need

There is a need to meet the CRC’s desired $240 million gain in seafood value through innovative technologies as scheduled in the CRC program 1. This proposal will contribute up to 47% of this CRC goal, as explained in the “background” section.

This application also meets the need to respond to the FRDC’s challenge 3 (response to demand, profitability) and FRDC priority “develop innovative processes for value-adding through
development” (genetic improvement will return greater profit per kg, or increase kgs for same infrastructure). Should the “Seafarm” transformational model be adopted by others, this will directly support the achievement of FRDC’s key performance indicators “at least two companies accessing new markets”, and “at least two entities utilizing improved stock from selective breeding”.

This application supports the stated and written need from the APFA, as a major stakeholder, to achieve a method to apply its R&D levy for the whole of the industry, for both P. monodon and F. merguiensis. The application makes the case that as a transformational model for profitability through innovative technologies this project delivers industry wide outcomes

Objectives

1. Understand if past and existing breeding practices led to significant inbreeding (more than 2% per generation) and, if substantial inbreeding has occurred and will continue to occur, develop options to track family pedigrees and so more effectively limit inbreeding
2. Estimate genetic heritabilities and correlations for commercially important traits. This information will be available for companies to develop efficient breeding programs
3. Determine if functional markers for a range of commercial traits are commercially feasible. If so, these makers will be available for companies to integrate into their breeding programs through a selection index

Final report

ISBN: 978-1-925983-34-0
Authors: Janet Howieson Guan Tay Steve Iaschi Karl Hansal and Wendy Newton
Final Report • 2013-12-04 • 13.01 MB
2009-773-DLD.pdf

Summary

Seafarm, at Cardwell, for most of the last 20 years, has been Australia’s largest prawn farm and the major producer of Banana Prawns. This producer wanted to understand and resolve the suspected inbreeding/ loss of diversity issues, and if necessary, redesign their breeding program to ensure it would be sustainable in the long term. This project attempted a “forensic” examination of a prawn selection program with the intention that the information would help make the program more sustainable and contribute foundation knowledge of what likely happens in such mass selection programs conducted by other companies on organisms with very high fecundities. It was also tested whether hepatopacreatic parvovirus (HPV) was under genetic control.

This project was able to provide genetic management information that promoted increased productivity at Seafarm, helping to revise their genetic breeding program to mitigate inbreeding and restore allelic diversity. The revised program has halted further inbreeding; increased the diversity in the crossed lined; lifted the growth of the outcrossed lines vs the inbred lines; and accelerated the rate of selection response.

SCRC: Seafood Success – Entrepreneurship & Innovation Program – Seafood CRC Professional Diploma

Project number: 2009-702
Project Status:
Completed
Budget expenditure: $0.00
Principal Investigator: Evan Douglas
Organisation: University of the Sunshine Coast (USC)
Project start/end date: 28 Feb 2009 - 27 Feb 2012
Contact:
FRDC

Need

Very few ideas are turned in commercial successes. A great many of them have potential and significant investment is usually directed to overcoming any technical issues that may be encountered. The resulting innovations still need to be commercialised. It is this commercialisation process that can make the difference between an innovation succeeding or failing. The good news is that there are a number of tools and techniques that can be learned that will increased the chance that an innovation can become a commercial reality.

The Seafood Success program is designed to focus on the commercialisation process - it will comprise seminars and workshops that deal with identifying the business models that are most appropriate for the innovation, the IP arrangements that need to be put in place, the marketing, financial and human resource considerations to name a few of the topics covered.

Participants will learn the skills and apply them to their own innovations and ideas to create a robust business plan that can be used to attract investment, by the SFCRC or others. The program will be self selecting, people with an idea and the enthusiasm and resources to support the development of a business plan will be able to participate. The CRC may also make participation a condition of investment in an innovative product or process.

Final report

ISBN: 978-1-925983-33-3
Author: Evan Douglas
Final Report • 2012-02-28 • 671.08 KB
2009-702-DLD.pdf

Summary

One of the original aims of the Seafood CRC was to create opportunities for industry (especially) and research participants to expand their knowledge and to encourage further education opportunities. To enable individuals to take innovative concepts right through to a business plan via an educational process that expanded their horizons with regards to what could be achieved would be ground breaking.

Very few ideas are turned into commercial successes. A great many of them have potential, and significant investment is usually directed to overcoming any technical issues that may be encountered. The resulting innovations still need to be commercialised. It is this commercialisation process that can make the difference between an innovation succeeding or failing. There are a number of tools and techniques that can be learned that will increase the chance that an innovation can become a commercial reality therefore a program focusing on the commercialisation process was required.

The program was delivered in two main formats, an eight month program over eight weekends (producing a business plan) and a four day program focussing more broadly on developing management and decision making skills. The eight month version attracted a smaller more narrowly focussed group of attendees but resulted in slightly higher outcomes in terms of overall satisfaction, impact and stronger ongoing networks, while the shorter version had broader appeal, attracted more attendees and still resulted in high levels of satisfaction and impact (but not as strong ongoing networks).

Seafood CRC: repositioning Australian farmed Barramundi in the domestic market

Project number: 2008-794.20
Project Status:
Completed
Budget expenditure: $284,000.00
Principal Investigator: Janet Howieson
Organisation: University of the Sunshine Coast (USC)
Project start/end date: 8 Nov 2009 - 30 Jul 2012
Contact:
FRDC
SPECIES

Need

During the past 5 years Australian farmed barramundi production has increased from 2,700 tonnes in 2003/04 to an expected 6,000 tonnes in 2008/09 while average farm gate prices have fallen. This coupled with the current global recession is impacting significantly on the profitability of the barramundi farmers.

There is an opportunity and need to develop and implement a repositioning strategy for the domestic market that enables Australian farmed Baramundi to be differentiated from its competitors and to deliver on consumer expectations for quality and price.

Objectives

1. To develop, trial and evaluate at least two repositioning strategies for Australian farmed barramundi on the domestic market.
2. At least two ABFA members undertaking consumer driven product and market development activities to support the repositioning strategies

SCRC: SCRC Honours Scholarship: Ying Ying Lee "Understanding the role of Kiss1/Kiss1r system in controlling the puberty in Yellowtail kingfish and Southern Bluefin Tuna"

Project number: 2008-785
Project Status:
Completed
Budget expenditure: $0.00
Principal Investigator: Abigail Elizur
Organisation: University of the Sunshine Coast (USC)
Project start/end date: 4 Dec 2008 - 4 Dec 2009
Contact:
FRDC

Final report

ISBN: 978-1-925982-42-8
Author: Ying Ying Lee
Final Report • 2009-12-05 • 2.36 MB
2008-785-DLD-PhD.pdf

Summary

The cDNA encoding for G-protein coupled receptor 54 (GPR54) was cloned from the brains of Southern Bluefin Tuna (SBT) and Yellowtail Kingfish (YTK). The SBT GPR54 has an open reading frame of 1134bp encoding a predicted 378 amino acid peptide, containing seven putative transmembrane domains, a 138 bp 5'UTR and 238 3'UTR. The partial YTK GPR54 cDNA contains 729 bp nucleotide sequence encoding 243 amino acid residues.

A RT-qPCR assay was developed for both SBT and YTK GPR54, as confirmed by primer specificity and high R2 values. The reference gene used, Hprt1, displayed consistent Cq values across most of the different sampling points, however some interaction between the reference gene expression and developmental stage was observed. GPR54 expression levels were determined for two cohorts, an immature group and a pubertal group, at two sampling points. RT-qPCR results showed that YTK brain GPR54 expression levels did not show sexual dimorphism and were significantly higher in the reproductively mature fish at a point past their peak spawning time compared with expression in the immature fish group.

The YTK gonad GPR54 expression level was significantly lower in males from the pubertal fish group during peak spawning time. In SBT, GPR54 expression profile in the brain and the gonad did not vary between immature and reproductively advanced fish. Analysis was carried out using both relative gene expression data and raw Cq. Alternative reference genes would need to be examined for the assay to be reliable across tissues and developmental time points.

SCRC: PhD : The effect of temperature on reproductive development in maiden and repeat spawning farmed Atlantic Salmon: Understanding the molecular basis for improved egg quality and survival

Project number: 2008-762
Project Status:
Completed
Budget expenditure: $0.00
Principal Investigator: Abigail Elizur
Organisation: University of the Sunshine Coast (USC)
Project start/end date: 14 Sep 2008 - 3 Feb 2011
Contact:
FRDC

Need

The economic viability of sea cage farming of Atlantic salmon is strongly influenced by the cost of
production of smolts. Understanding the mechanisms that contribute to, or cause reproductive failure in
spawning fish is an essential component of reducing those industry production costs, and at a broader
level, ensuring that there are sufficient smolts produced each year to maintain industry production. The
issue has been identified as an industry priority with the stated SALTAS aim of reducing the reliance on
repeat spawning fish for egg production. The potential cost of failing to solve the problem is high. The
survival of eggs to the eyed embryo stage can be as low as 30-50%, compared with 80% for eggs from
best performing fish (SALTAS data). Modelling of this cost gives direct increases in smolt production
costs of $225,000 per annum, but a potential industry shortfall in production terms of $15-20 million per
annum.

The knowledge gained through this research will be applied in a real life context to the Atlantic salmon industry in Australia to overcome a real and immediate industry bottleneck, and provide information to assist in large scale production procedures. It will provide important new information regarding the endocrine regulation of egg quality, information that could be highly relevant to other finfish industries, such as tuna and kingfish.

In addition to the direct benefit of this research to the Salmon industry, the investigation of the impact of temperature on reproductive process in fish is relevant to environmental studies concerning the impact of global warming on biological processes, with the Atlantic salmon being an example of a fish that has been translocated from a colder environment (Europe) to the warmer Tasmania.

Final report

ISBN: 978-1-925982-26-8
Author: Kelli Anderson
Final Report • 2012-01-13 • 3.42 MB
2008-762-DLD-PhD.pdf

Summary

Tasmanian Atlantic Salmon (Salmo salar) broodstock can encounter temperatures above 20°C, which has a marked negative effect on reproductive development. Broodstock management strategies are needed in order to maintain egg quality in the face of thermal challenge either due to seasonal fluctuation or climate change, where temperatures are expected to rise even further. In Tasmania, the Atlantic Salmon industry uses maiden fish for approximately 75 % of egg production due to their smaller size and the lower cost associated with their husbandry relative to repeats. However, maidens appear to be more susceptible than repeat fish to the effects of elevated temperature. The salmon industry's ability to cope with rising temperature is hindered by the lack of understanding of the effects of thermal challenge on the endocrine system, which ultimately determines egg quality.

Therefore the aims of the research were to:

  1. Determine how temperature influences endocrine function and reproductive development in maiden and repeat spawning female Atlantic Salmon;
  2. Understand the molecular mechanisms that determine egg quality in broodstock maintained at elevated temperature; and
  3. Develop management strategies to maintain endocrine function and egg quality under thermally challenging conditions

The research provided evidence that female maiden Atlantic Salmon are more susceptible to the effects of elevated temperature and that thermal impairment of endocrine function occurs at multiple levels of the reproductive axis. Treatment with various hormonal therapies showed mixed results and suggest that it is difficult to overcome the impacts of higher temperature on the reproductive axis using a single hormonal treatment. Hormonal therapies in the future should simultaneously stimulate vitellogenesis and zonagenesis in order to maintain egg quality. It is evident that the method of hormonal delivery is of considerable importance and implantation techniques also warrant further investigation.

Seafood CRC: The advancement of reproductive development in Southern Bluefin Tuna using hormonal manipulations of kisspeptin, the gatekeeper of puberty

Project number: 2008-745
Project Status:
Completed
Budget expenditure: $117,391.67
Principal Investigator: Abigail Elizur
Organisation: University of the Sunshine Coast (USC)
Project start/end date: 14 Aug 2008 - 13 Aug 2011
Contact:
FRDC

Need

Commercial in confidence. To know more about this project please contact FRDC.

Objectives

Commercial in confidence

People development program: 2011 FRDC International Travel Bursaries- Abigail Elizur

Project number: 2008-314.27
Project Status:
Completed
Budget expenditure: $5,000.00
Principal Investigator: Abigail Elizur
Organisation: University of the Sunshine Coast (USC)
Project start/end date: 31 May 2011 - 29 Sep 2011
Contact:
FRDC

Need

This application is for an international bursary to attend a two week intensive course on next generation sequencing.

The course, held by Michigan University, will cover all aspects of bioinformatics analysis needed for transcriptomes and genome sequencing, as well as train in the analysis of sequences the course participants bring themselves.

At USC we have moved into the field of transcriptome and genome sequencing, and now have transcriptomes of 8 prawn tissues, pearl oyster, 2 fish tissues, and we are about to embark on sea cucumber and edible oyster sequencing, as well as tuna genomics. While capable of doing the basic analysis, this resource offers much more, and this course would train me for such analysis.

There are not many groups in Australia doing wide genome analysis of aquaculture species, and this training and materials provided in the course would put me in a position to not only service my FRDC and CRC grant industry partners, but also to train interested parties in Australia who are interested in expanding into this field. USC is investing in building up its aquaculture genetics portfolio, and this training opportunity would add to the strength of the group.

Elizur would extend her knowledge gained in this travel to the aquaculture community through lectures at a CRC or industry forums where there is potential for uptake. She will communicate with the CRC about opportunities to present to the student forum where molecular approaches could be adopted to address some of the research questions.

Objectives

1. To take part in a two week intensive course on analysis of next generation sequencing
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