78 results

Aquatic Animal Health Subprogram: vibrios of Aquatic Animals: development of a national standard diagnostic technology

Project number: 2001-628
Project Status:
Completed
Budget expenditure: $226,585.00
Principal Investigator: Jeremy Carson
Organisation: University of Tasmania (UTAS)
Project start/end date: 13 Oct 2001 - 1 Jul 2007
Contact:
FRDC

Need

Diagnostic competence in the identification of Vibrio species is of growing concern with the expansion and diversification of aquaculture in Australia. The urgent need to improve diagnostic capacity has been identified as an essential goal in the SCFA Research Priorities for Australian Fisheries & Aquaculture. Under AQUAPLAN improved diagnostic capacity in aquatic animal diseases was also identified as a major national goal under Projects 3.1 Surveillance & Monitoring, 4.2.9 Diagnostic Resources, 6.2.3 Development of New Diagnostic Tests. The National Workshop on Aquatic Animal Health: Technical Issues (FHMC 1999) identified improved diagnostic capacity for Vibrio species as a matter of priority. More recently, Aquatic Animal Industry Stakeholders nominated the identification of Vibrios as a priority need for funding under the Federal Government Budget Initiative, 'Building A National Approach To Animal And Plant Health' (AQUAPLAN, Business Group (FHMC Sub-Committee, Steering Committee of the FRDC Aquatic Animal Health Sub-Program).

Of major concern is the drive to establish health surveillance programs for aquatic animals. A surveillance program already exists for salmonids in Tasmania and similar programs for abalone in Tasmania, Victoria, South Australia and Western Australia have been identified as a priority need by the FRDC Abalone sub-programme. Similarly, a health surveillance program has been proposed for rock lobster. A major weakness however of these schemes is the lack of diagnostic capacity in veterinary laboratories servicing these health programs (Anon 1999). Given that Vibrio species form over 60% of the bacterial flora associated with these major aquaculture species the usefulness of these proposed surveillance schemes is severely limited.

Competence in identification of Vibrio species is an essential pre-requisite in any surveillance program. It provides the basis to assess the significance of findings, is a means of monitoring populations for the emergence of specific pathogens and underpins successful disease management strategies through the selection of appropriate antibiotics, probiotics or vaccines.

The difficulties identifying Vibrio species were highlighted through a National Fish Disease Bacteriology Workshop (FRDC 00/149). All participating laboratories confirmed the low success rate identifying Vibrio species isolated from aquatic animals and found most identification systems either inefficient, cumbersome or unreliable. Participants endorsed strongly the need to improve diagnostic capacity for Vibrio species.

References
Anon (1999) Gap Analysis of Research for Australian Fisheries and Aquaculture. Report for the Research Committee, Standing Committee for Fisheries and Aquaculture
FHMC (1999) Report of the Workshop on Aquatic Animal Health: Technical Issues. 7-9 December 1998, Attwood, Victoria

Objectives

1. Determine the range of Vibrio species associated with farmed fish, shellfish and crustacea in Australia
2. Undertake a definitive numerical taxonomic analysis to describe and characterise the phenotypes of aquatic animal Vibrios in Australia
3. Develop practical, robust phenotypic identification systems for Vibrio species using computer assisted identification software
4. Develop and implement PCR gene probes for the rapid identification of key bacterial pathogens
5. Produce an ANZSDP for the Identification of Vibrio species in aquatic animals
6. Technology transfer workshop for state veterinary diagnostic microbiologists

Final report

ISBN: 1-86295-368-6
Author: Jeremy Carson

Aquatic Animal Health Subprogram: development of diagnostic capability for priority aquatic animal diseases of national significance:spawner-isolated mortality virus

Project number: 2001-625
Project Status:
Completed
Budget expenditure: $150,270.00
Principal Investigator: Leigh Owens
Organisation: James Cook University (JCU)
Project start/end date: 30 Jan 2002 - 21 Apr 2005
Contact:
FRDC

Need

Midcrop mortality syndrome has caused significant losses in Queensland and New South Wales prawn farms. Estimated losses over the period of 1994-98 are $44 million (CRC Project A1.4). Two viruses are thought to be involved, (LOV/GAV) and (SMV). We have focused on detecting and controlling SMV. A survey of 909 faecal samples from spawners correlated SMV PCR-positive results with a decrease in productivity. Spawners with PCR-positive faecal samples produced PLs with a 23% reduced survival compared to PCR-negative spawners. A similar negative relationship between PCR-positive spawners and the survival of progeny has been shown with white spot syndrome virus (WSSV) in Asia. We have already developed a robust PCR for use with faecal samples. We propose to refine the technique using ELOSA, and to produce a non-infectious positive control which will make it suitable for non-specialist laboratories and automation. Screening at the hatchery provides maximal downstream effect on controlling disease in the industry. There is a need to develop time and cost-effective screening tools for SMV to reduce the mortality caused by this virus. This application addresses that need.

Furthermore, SMV has been listed by OIE (Office International des Epizooties, 2000) as an "other significant disease", so Australia has reporting obligations as a signatory to the regulations of the World Trade Organisation. Development of a diagnostic kit for SMV will make it easier for more laboratories to test for SMV and therefore help Australia meet these obligations.

Objectives

1. Produce a non-infectious, SMV-positive control for use by other laboratories and for inclusion in a diagnostic kit.
2. Develop a kit based on ELOSA (enzyme-linked oligosorbent assay) to detect SMV infections in spawners and post-larvae.
3. Screen spawners and post-larvae in commercial hatcheries using the SMV ELOSA to establish prevalence and to correlate SMV detection with available production parameters.

Final report

World's Best Practice in Environmental Management of Shrimp Farming

Project number: 2000-196
Project Status:
Completed
Budget expenditure: $7,500.00
Principal Investigator: Simon Wilkinson
Organisation: Department of Agriculture Fisheries and Forestry
Project start/end date: 17 Dec 2000 - 8 Nov 2003
Contact:
FRDC

Need

Domestic need

Within Australia, the regulatory frameworks for ecologically sustainable development (ESD) are at a critical point in their development. At the Commonwealth level, aquaculture will be directly affected by amendments to Schedule 4 of the Wildlife Protection (Regulation of Exports and Imports) Act 1982 (WPA); by the introduction of the Environment Protection and Biodiversity Conservation Act 1999 (EPBC); and in Queensland, by the introduction of the Great Barrier Reef Marine Park (Aquaculture) Regulations 1999. Environmental legislation regulating shrimp culture in Queensland is also currently under review.

The Standing Committee on Fisheries and Aquaculture (SCFA) has established a Working Group on ESD. The working group aims to develop ESD indicators for assessing fisheries and aquaculture in environmental, social and economic contexts. It is anticipated that the ESD assessment process developed through this work will form the basis for Environment Australia’s assessment of fisheries and aquaculture under WPA and EPBC. The first aquaculture case study was held on shrimp farming, 3-4 October 2000.

The Australian Prawn Farmers Association (APFA) convened a National Shrimp Farming Environmental Management Workshop 24-25 May 2000. One of the outcomes of the workshop was for Australian industry “…by 2010, to have technology and farming practices and strategies that achieve worlds best practice and nil tangible water quality impacts…APFA to establish a comprehensive strategy for ESD by fully supporting SCFA Case Study for developing ESD Indicators.”

Given these developments, the expert consultation is ideally placed to facilitate the development of appropriate ESD policy, legal frameworks and good management practices for shrimp culture in Australia. It is expected that the outcomes of the consultation will provide guidance and a common platform for the policy development currently being undertaken by SCFA, Environment Australia and the Australian Prawn Farmer’s Association. This will occur through the interaction of key Australian policy officers with international experts at the consultation, and through the guidelines arising from the workshop.

International need

In December 1997, FAO convened the Technical Consultation on Policies for Sustainable Shrimp Culture. This consultation brought together government delegates and observers from 12 countries of Asia and America accounting for about 90 % of the global production and major consuming countries.

The Consultation noted that the achievement of sustainable shrimp culture is dependent on effective government policy and regulatory actions, as well as the co-operation of industry in utilising sound technology in its planning, development and operations. In this regard, the Consultation recommended that: FAO convene expert meetings to elaborate best practices for shrimp culture and the legal and other regulatory instruments for coastal aquaculture.

Objectives

1. Provide a recognised international forum for discussion on the promotion of sustainable shrimp culture practices, and related institutional and legal instruments
2. Continue facilitating the process of consensus-building among major stakeholders concerned with shrimp culture development and management
and
3. Identify/determine avenues, as well as specific benefits and limitations, for the development and implementation of Good Management Practices and Good Legal and Institutional Arrangements leading to improvements in shrimp aquaculture management practices at farm and institutional levels.
4. Development of a range of guidelines to implement good management practices for sustainable prawn farming

Final report

ISBN: 92-5-104730-8
Author: Simon Wilkinson

Sustainable Penaeus monodon (tiger prawn) populations for broodstock supply

Project number: 1999-119
Project Status:
Completed
Budget expenditure: $354,186.60
Principal Investigator: Neil Gribble
Organisation: Department of Primary Industries (QLD)
Project start/end date: 11 Jul 1999 - 30 May 2004
Contact:
FRDC

Need

The overriding needs for this project are to maintain a sustainable fishery for P. monodon to ensure broodstock supply to the Australian prawn farming industry.

This project requests an early start in order to sample the two recruitments into the main fishery, and to address the lack of broodstock, to supply the doubling of the current size of the P. monodon farming industry in the following 1 to 2 years.

1) Little published information is available on wild broodstock biology and ecology, presumably because P. monodon is really an incidental catch. Nevertheless, information on the species has been collected in logbook programs (eg. QDPI, Bill Izard and Jim Brownings tagging of adult P. monodon in the Cairns/Innisfail regions), and in many studies directed at other penaeids. Information on the species is thinly distributed over a large number of datasets, uncollated and unanalysed. This study will undertake the collation of information over a 6 month period at the beginning of this project to assisst in refining later stages of this project.

2) Although considerable effort and resources have been allocated to the full domestication of P.monodon, both nationally and internationally, the commercial production of closed broodstock is as yet not viable. The P. monodon prawn farming industry is currently dependent on wild caught broodstock and will be for the foreseeable future. Pressures are building for the known wild stocks to supply an expanding local and international aquaculture market that is expected to double or triple in the following one to two years, yet little is known of the size and sustainability of currently sourced Queensland stocks of this species. There is a clear and present need to establish the size of the resource to maintain the existing industry and further establish a regular and sustainable supply of broodstock to ensure national expansion and viability of P. monodon prawn farming.

3). There is a need for alternative capture methods and associated stress testing to be assessed. This is required by commercial broodstock collectors and the Northern Territory fishery to capture the highest quality broodstock, source broodstock from different habitats, manage specific fisheries and to lessen environmental impacts of the fishery.

4). There is a need for an economic assessment of potential gains to the industry from optimising the quantity, quality and timing of the supply of P. monodon broodstock.

Attachment 1 provides a dissection of the research problem.

Objectives

1. Collate fisheries information currently available on P. monodon across northern Australia from grey literature, fisheries databases, research projects and from indigenous communities.
2. Define the distribution of adult P. monodon stocks and habitats.
3. Define the distribution of juvenile P. monodon stocks and habitats.
4. Determine seasonal patterns in P. monodon population dynamics (abundance, population structure).
5. Identify P. monodon biology (recruitment, movement, growth and reproduction) in Queensland.
6. Examine alternative capture techniques and the associated stress testing of caught broodstock, in particular for inshore and shallow water habitats which may contain useable quantities of currently unexploited broodstock.
7. Conduct economic cost/benefit analyses of various fishing patterns, capture techniques and handling protocols.

An investigation into the feasibility of producing a commercially acceptable prawn stock from the discarded heads of commercially processed prawns.

Project number: 1998-419
Project Status:
Completed
Budget expenditure: $14,515.00
Principal Investigator: Jason Hancock
Organisation: Department of Primary Industries (QLD)
Project start/end date: 19 Jun 1999 - 31 Dec 2000
Contact:
FRDC

Need

This development will benefit both the fishing and aquaculture industry through a better utilisation of prawn waste. It will involve taking a generally discarded seafood waste and produce a value added product. It will aid the seafood processors by reducing the costly expense of disposing of processing waste, assist in waste management and potentially produce a new market for unwanted material. Presently only small quantities of prawn heads are utilised in further processing, being sold for little or no profit. Generally prawn heads are regarded as an expense to the processor. This expense is increasing as greater pressure is being placed on manufacturers with regards to the disposal of biological waste.

This project will allow manufacturers access to detailed technical information on the processing requirements for producing a prawn stock as well as the information on the export market and the domestic market potential. This project will allow processors to investigate an additional product to manufacture without the additional costs of product development.
The success of this project is aimed at generating interest in the utilisation of prawn waste into value added food products.

Objectives

1. To develop a (prototype) prawn stock (liquid and powder) at the Centre for Food Technology from the discarded heads of commercially processed prawn species P.monodon.
2. To evaluate suitable forms of packaging for the prototype stock . Eg. UHT (fibre board plastic), canned (retorted) and plastic bags (fresh / frozen).
3. To conduct pilot scale trials and production trials.
4. To determine the shelf life of the stock produced from processing trials..
5. To evaluate the potential of other species of prawn for use as raw material in the prawn stock.
6. To test market prawn stock samples in Japanand/or domestically if product is not suitable for Japan.

Final report

Author: Jason Hancock
Final Report • 2001-10-09 • 1.15 MB
1998-419-DLD.pdf

Summary

The Fisheries Research and Development Corporation project 98/419 ‘An Investigation into the feasibility of producing a commercially acceptable prawn stock from the discarded heads of commercially processed prawns’ has successfully completed Objective 1, to develop a (prototype) prawn stock (liquid and powder) at the Centre for Food Technology from the discarded prawn heads of commercially processed prawn species Penaeus monodon.

Formulations of several prawn and seafood stocks were gathered from both Hiromi Ishikawa of Akebono Services and from a literature search.  Hiromi Ishikawa owns a leading seafood restaurant in Japan and therefore has first hand knowledge of the food service industry and what chefs require for producing seafood stocks. Preliminary trials utilised the recipes gained from both Hiromi and the literature search to establish a starting formulation.  The early development work identified the potency of the prawn flavour when extracted from the prawn heads, and also determined if there were any off flavours that developed from the prawn heads during processing.

Results from trials 1,2 and 3 indicated the combination of several fresh ingredients utilised in the formulations masked any unacceptable flavours produced during the manufacture of the prawn stock liquid. Some of these masking ingredients included tomato puree, carrots, onions, celery, lemon juice and white wine.  The combined effect of these ingredients was a clean but mild tasting prawn flavour with no off flavours or odours.

People

Symposium on parasitic diseases of aquatic animals: 10th International Congress of Protozoology

Project number: 1997-336
Project Status:
Completed
Budget expenditure: $8,350.00
Principal Investigator: Bob J. Lester
Organisation: University of Queensland (UQ)
Project start/end date: 25 Apr 1997 - 4 Jan 1999
Contact:
FRDC

Need

The overseas speakers have agreed to come, speak at the Congress and speak to at least one other group while in Australia providing there is some assistance with their travel expenses. This is a great opportunity for members of the fishing and aquaculture industry to hear about latest developments in disease research. The proposed presence of these speakers at the Congress has already attracted other experts in marine disease to come to Australia for the Congress and these also will be meeting with special interest groups while here.

Objectives

1. The objective is to bring three overseas experts to explain about current developments in marine parasitology that relate to wild and caged tuna and other fish, prawns and oysters.

Final report

Author: Bob Lester
Final Report • 1998-11-18 • 519.90 KB
1997-336-DLD.pdf

Summary

A symposium on protozoan diseases of aquatic animals was planned as a feature of the 10th International Congress of Protozoology.

Speakers invited for the symposium were: Dr Mike Hine, NIWA, NZ, an expert on oyster and fish diseases, Prof. Tim Flegel, Mahidol University, Bangkok, an expert on prawn diseases, Dr El-Matbouli, University of Munich, an expert on myxosporeans of fish, and Prof. Bob Lester, University of Queensland, to talk on white spot disease in fish and to chair the symposium.

High quality eggs and nauplii for the Australian prawn industry.

Project number: 1995-166
Project Status:
Completed
Budget expenditure: $516,346.00
Principal Investigator: Michael Hall
Organisation: Australian Institute Of Marine Science (AIMS)
Project start/end date: 30 Dec 1995 - 17 Jun 2003
Contact:
FRDC

Objectives

1. To determine the physiological requirements for successful & high quality egg production: Vitellogenesis and its physiological control.
2. To identify & chronicle the substances which are accumulated during egg development and their importance in egg viability and larvae survival: Egg quality and packaging.
3. Identify & chronicle differences in substances between wild caught spawners and pond reared spawners.
4. Compare egg quality between wild and captive-reared broodstock.
5. Demonstrate value of supplementing broodstock diets with optimal concentrations of carotenoids.
6. Demonstrate value of supplementing broodstock diet with ecdysone.

Final report

ISBN: 0-642-32260-0
Author: Michael Hall
Final Report • 2003-05-30 • 2.33 MB
1995-166-DLD.pdf

Summary

Prawn farming is the most valuable aquaculture sector in Queensland and is a priority development industry for the State Government. Marine prawns have provided the major growth in this industry with a value of $37 million in 2000/01, or over 66% of the value of Queensland aquaculture. Nevertheless several issues need to be addressed to ensure the industry continues to grow and is economically and environmentally sustainable. 

The supply and performance of broodstock is seen as the weakest link in the production cycle, both overseas and nationally, and is viewed as a major research priority by the Australian Prawn Farmers Association (APFA). Presently the marine prawn sector is dominated by Penaeus monodon production, the broodstock of which are obtained from the wild fishery in a few near-shore coastal areas in northern Queensland. Recent screening of wild-sourced broodstock for viruses has revealed that nearly all of them, from the traditional areas of supply, are carriers of potentially pathogenic forms   of   viruses.  In response, the industry has developed a strategic goal of becoming a true farming sector by shifting its reliance on wild broodstock toward totally closed life cycle production. Research providers and industry are now committed to developing domesticated broodstock that will eventually be used in selective breeding programs to produce specific strains or breeds.

This project placed special emphasis on strategic issues, to develop solutions to current and future challenges for prawn hatcheries, and applied issues, with effort directed towards clear and identifiable objectives in improving broodstock reproductive performance. Strategic issues included developing specific research tools to investigate some of the basic mechanisms that underpin reproductive capability and its physiological control in P. monodon (Objectives 1 and 2). This information is essential if informed knowledge-based improvements in broodstock management are to develop in a vigorous manner. Applied issues included the objective of comparing reproductive performance of captive-reared broodstock that had been grown to sexual maturity in either tanks or ponds (Objective 3). The final objective was directed to examining supplementation of specific compounds in broodstock maturation diets and their impacts on subsequent reproductive performance
(Objective 4).

Keywords: Penaeus monodon, aquaculture, egg quality, fertility.

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