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Rock Lobster Post Harvest Subprogram: rock lobster autopsy manual

Project number: 1999-202
Project Status:
Completed
Budget expenditure: $34,490.00
Principal Investigator: Louis Evans
Organisation: Curtin University
Project start/end date: 5 Sep 1999 - 7 Jan 2005
Contact:
FRDC

Need

With the increasing interest of lobster processors to keep lobsters for extended time periods prior to sale, and of the lobster industry in general in lobster aquaculture, there is a need for information on lobster health management. It is well recognised that a range of diseases and conditions occur in wildstock lobsters (Evans & Brock, 1994) and that health management problems are likely to be encountered in enhancement and aquaculture of spiny rock lobsters. In order to prevent disease outbreaks, or effectively deal with such conditions should they occur, reference material is required for use by personnel involved in enhancement and aquaculture programs. At present there is very little published information on health management of spiny rock lobsters held in captivity. A valuable first step to address this need would be the publication of a manual describing the approach to be used to investigate lobster health problems along with photographic material on disease conditions likely to occur in captive lobsters. Such a manual would be used by operators of holding/culture facilities or by fish health personnel involved in disease investigations. An autopsy manual with illustrations of common parasitic and disease conditions found in Australian lobsters would also be of interest to lobster fishers.

One of the conditions most likely to occur in captive lobsters is bacterial infections by opportunistic pathogens, those bacteria which are ubiquitous and only cause disease when the lobster is weak or when a particularly virulent strain of bacteria is encountered. It is well known that crustaceans can harbour bacteria in their body fluids apparently without causing the animal any harm. Whether the presence of bacteria in the hemolymph of lobsters is indicative of the likelihood of disease outbreaks and future stock mortality is not well understood. In addition to publication of a lobster autopsy manual, this project will provide information which will contribute to understanding and managing bacterial infections in captive lobsters. The information will be obtained from bacterial challenge experiments conducted on lobsters held in aquaria in the laboratory. These experiments will also provide descriptive material for inclusion in the lobster autopsy manual.

The techniques and information described in the autopsy manual can be used to investigate possible problems arising from mortalities of unknown causes, as well provide fishers and factory staff with a reference to consult when lobsters with health problems are encountered during fishing and processing. The autopsy manual will be of value to fish pathologists for use in disease diagnosis as well as to fishers, fish processors and researchers.
Evans L.H. & Brock J.A. 1994. Diseases on spiny lobsters. In "Spiny Lobster Management (Ed. B.F. Phillips, J.S. Cobb & J. Kittaka) pp 461-472. Blackwell Scientific Publications.

The autopsy manual will include details of approach to be followed in conducting an autopsy on a lobster, descriptions of assay methodology, normal ranges for selected immune and physiological parameters, expected deviations from normal in lobsters subjected to excessive postharvest stressors, photographs of gross and microscopic lesions and descriptions of pathogens observed in apparently healthy lobsters as well as in moribund lobsters.

Objectives

1. The publication of an autopsy manual to be used in the lobster industry. The manual will include details of approach to be followed in conducting an autopsy on a lobster, descriptions of assay methodology, normal ranges for selected immune and physiological parameters, expected deviations from normal in lobsters subjected to excessive post harvest stressors, photographs of gross and microscopic lesions and descriptions of pathogens observed in apparently healthy lobsters as well as in moribund lobsters.

Final report

Author: Louis Evans
Final Report • 2003-11-18 • 7.46 MB
1999-202-DLD.pdf

Summary

Data and information on lobster host defense responses, disease conditions, dissection procedures and laboratory investigation methods were obtained from the FRDC projects 94/134. 07, 96/344, 96/345 and 98/302 and other sources and collated into a publication on the nature and investigation of lobster diseases. Contributions to the book were sought from lobster researchers and fish health personnel from Australia and New Zealand. Following collation and editorial review, the individual chapters were circulated for review and comment. The publication, titled ‘A review of lobster diseases, their investigation and pre-disposing factors’ was published in 2003 and contains the following chapters:

  1. Lobster health and disease concepts (Louis Evans)
  2. Responses to environmental stressors (Louis Evans)
  3. Lobster autopsy procedure and immune assay methods (Louis Evans, Seema Fotedar and Anne Barnes)
  4. Collection and handling of blood samples from spiny lobsters (Brian Paterson, Patrick Spanoghe and Glen Davidson)
  5. Reagents and methodology for immune function tests (Louis Evans & Seema Fotedar)
  6. Review of adult lobster diseases (Frances Stephens, Louis Evans, Seema Fotedar and Brian Jones)
  7. Disease conditions of larval and juvenile spiny lobsters (Ben Diggles and Judith Handlinger)
  8. Biosecurity and food safety issues (Frances Stephens)

3rd International Lobster Congress

Project number: 1998-341
Project Status:
Completed
Budget expenditure: $71,825.00
Principal Investigator: Roger Edwards
Organisation: South Australian Rock Lobster Advisory Council (SARLAC)
Project start/end date: 21 Jun 1998 - 30 Dec 2000
Contact:
FRDC

Need

The changing marketplace and production profile of rock lobster internationally dictates that the Australian rock lobster industry applies state of the art technology in management, research and development, if we are to maintain a competitive position and grow the value of the limited resource.

At present no vehicle exists to allow industry an effective interchange of ideas or to consider leading edge research and development from around the globe. Essentially the Australian lobster industries operate in isolation, disjointed by State borders.

The Tri-State Southern Rock Lobster Conference has historically seen ad hoc attempts made to bring industry together. The conference has evolved into a quasi national event with representatives from most States and New Zealand in attendance, although the primary focus on the jasus edwardsii lobster remains a deficiency.

An outcome of the most recent Tri-State Conference, was a call for a National Lobster Conference including all States and species not covered, to establish a forum for industry Australia wide to make a start at building a national focus on management, marketing, development and problem solving.

At present information flow in these areas are localised trickles at best and the industry has a responsibility to upgrade its role in information exchange.

Objectives

1. Provide an International Class Lobster Industry Congress
2. To Ensure a Financially Viable Event

Rock Lobster Enhancement and Aquaculture Subprogram Project 5: determination of the optimum environmental and system requirements for juvenile and adult rock lobster holding and grow-out

Project number: 1998-305
Project Status:
Completed
Budget expenditure: $247,084.00
Principal Investigator: Mike C. Geddes
Organisation: University of Adelaide
Project start/end date: 28 Jun 1998 - 14 Sep 2002
Contact:
FRDC

Need

The need for a coordinated sub-program, and research projects focussing on collection and neutrality, nutrition, health and system design and handling has been outlined in the background to this submission. Further evidence of the need for this and the other sub-program projects includes:

Project 1: COORDINATION AND SUB-PROGRAM MANAGEMENT: At a planning workshop in Hobart in 1997, an open forum of all participants identified 21 issues of concern to the aquaculture of rock lobsters. These were condensed into five major issues with each major issues condensed examined in detail by a discussion group. One of the five major issues was project management. Based on the range of research issues and other programs related to rock lobsters, well facilitated project management was considered a fundamental priority. With increasing demands being placed on scientists by their host organisations, the role of Sub-program Leader in addition to project commitments can be impossible to fulfil adequately. A dedicated Sub-program Leader will ensure the Sub-program runs effectively and objectives are delivered on time to the industry.

Project 2: COLLECTION AND NEUTRALITY: Before any large scale commercial on-growing of postlarvae is permitted, it will be necessary to establish what effect large scale harvesting of pueruli might have on the wild stock. A second critical need to the success of any commercial venture into rock lobster postlarval growout is that techniques be developed to harvest huge quantities of healthy pueruli. Research is needed to estimate the likely impact of large-scale harvesting of puerulus on the commercial fishery and to establish methods and equipment necessary to catch large quantities of pueruli in the most cost-effective way.

Project 3: NUTRITION: In Australia, opportunities to value add to the wild catch of lobsters or to on-grow juveniles taken from the wild is seriously constrained by the lack of a cost-effective and efficacious rock lobster feed. This contrasts with the developing industry in New Zealand where waste from the large mussel industry is an available and inexpensive source of feed. If feed comprises 40-50% of rock lobster production costs as is the case in other aquaculture industries (prawns, finfish), the development of a suitable manufactured feed is crucial for the successful establishment of rock lobster aquaculture in Australia.

Project 4: HEALTH: Due to the infancy of rock lobster aquaculture, disease conditions associated with production are poorly understood. Similarly, the prevalence of disease conditions in wildstock and their likely impact in aquaculture systems or extended holding systems has yet to be determined. While other factors associated with the establishment of rock lobster aquaculture are perceived as a higher priority, it is recognised that health monitoring and the early identification of diseases that may affect production is critical. In the short term, there is a need to establish a mechanism for the monitoring of disease conditions of juvenile and adult lobsters in land-based and sea-based holding systems. This will not only provide industry with a means of assessment of moribund lobsters, but will facilitate the identification of health research priorities.

Project 5: SYSTEM DESIGN AND HANDLING: Rock lobster fisheries throughout the world are generally fully or over-exploited while market demand remains very high with this product positioned at the premium end of the crustacean market spectrum. The proposed research will assist in increasing supply of this valuable product in a sustainable way and will consequently decrease pressure on wild populations. System design and basic husbandry information must be completed in conjunction with health and nutrition research as these factors combine to influence the efficiency of production.

Objectives

1. Assess the interactions between stocking density and feed delivery system on maintaining and improving condition and on the growth performance of adult rock lobsters in existing sea-based holding systems in different seasons.
2. Determine the effects of temperature , salinity and photoperiod on the growth rate and survival of juvenile rock lobsters in existing land-based holding systems.
3. Determine the effects of stocking density and shelter on the growth rate and survival of juvenile rock lobsters in existing land-based holding systems.
4. Evaluate existing system design and management regimes for land-based captive grow out of juvenile rock lobsters and for sea-based holding of adult rock lobsters.

Final report

Rock Lobster Enhancement and Aquaculture Subprogram Project 3: feed development for rock lobster aquaculture

Project number: 1998-303
Project Status:
Completed
Budget expenditure: $135,028.00
Principal Investigator: Kevin C. Williams
Organisation: CSIRO Oceans and Atmosphere Hobart
Project start/end date: 28 Jun 1998 - 18 Oct 2001
Contact:
FRDC

Need

Commercial in confidence. To know more about this project please contact FRDC.

Objectives

Commercial in confidence

Rock Lobster Enhancement and Aquaculture Subprogram: propagation of rock lobster - development of a collaborative national project with international partners

Project number: 1998-300
Project Status:
Completed
Budget expenditure: $26,797.29
Principal Investigator: Piers Hart
Organisation: University of Tasmania (UTAS)
Project start/end date: 7 Oct 1998 - 30 Jun 2001
Contact:
FRDC

Need

Expansion of output from the rock lobster fishery cannot be achieved using traditional fishing methods as most wild stocks are already fully exploited. It is therefore highly desirable to develop an aquaculture technique that can increase the long term production of rock lobster, in a sustainable manner.

It is presently not possible to produce puerulus from eggs on a large scale although it has been achieved in the laboratory in both New Zealand and Japan and there is extensive research being conducted in the USA. Very little information on puerulus production exists in the literature. If the problem of large scale consistent producton of puerulus is to be solved, then it needs a collaborative approach. If Australia is to benefit from the potential market for cultured rock lobster, then it must be in a position to design and import culture technology quickly.

At present there is very limited activity in Australia, the only research being conducted on rock lobster propagation is in Tasmania. However, there are:
a: opportunities for tapping overseas experience; and
b: nodes of expertise in Australia which require better co-ordination

The full collaborative project required to achieve the goal will be highly complex with a number of components and organisations. These organisations control major resources that could be co-ordinated and brought to bear on the problem of rock lobster propagation.

This proposal is seen as a fundamental first step in bringing together the groups and developing the project. FRDC funding is required for this task in order to provide a national focus and better cohesion between groups.

Objectives

1. Identify potential international and national, research & industry partners who are interested in collaborative research into culture of rock lobster from eggs
2. Identify barriers to the development of commercial culture of rock lobster in Australia and determine the R&D required to overcome these barriers
3. Identify what resources (expertise, facilities, funds) are required to conduct the necessary R&D
4. Develop a research plan mapping out R&D projects, the collaborative partners, timelines and resources.
5. Develop a collaborative, multi-institutional project proposal for FRDC

Rock Lobster Post-Harvest Subprogram: physiological studies of stress and morbidity during post-harvest handling and storage of western rock lobster (Panulirus cygnus)

Project number: 1996-345
Project Status:
Completed
Budget expenditure: $699,193.00
Principal Investigator: Brian Paterson
Organisation: Department of Primary Industries (QLD)
Project start/end date: 28 Jun 1996 - 19 Jan 2002
Contact:
FRDC

Objectives

1. Identify key physiological stress parameters that either describe stress levels and/or predict likely further mortality in lobsters after harvest and apply these parameters in studies aimed at improving post-harvest handling practises. This overall objective will be achieved by...
2. (Sub-objective 1) Obtaining baseline measurements of physiological parameters in resting undisturbed lobsters, with reference to interactions between season and locality and the effects of moult stage and other biological variables
3. (Sub-objective 2) Identifying physiological parameters, through field studies aimed at studying the effect of harvest and post-harvest handling on lobsters, which can be used to evaluate deviations from baseline values (stress responses) in captive lobsters.
4. (Sub-objective 3) Identifying physiological parameters, through controlled laboratory experiments using identified stressors (from 3. above) which can be used to evaluate deviations from baseline values (stress responses) in captive lobsters.
5. (Sub-objective 4) Develop simple methods of measuring one or more of the stress parameters identified in sub-objectives 2 and 3 for use in lobster processing factories in the evalutation of stress levels in selected lobster shipments
6. (Sub-objective 5) Apply the results and understanding of harvesting and post-harvest handling gained from field work in sub-objective 2, and the stress parameters identified in sub-objectives 2 and 3 in a study or studies of lobster postharvest handling practices aimed at developing improved post-harvest procedures
7. (Sub-objective 6) Use the findings of earlier sub-objectives to make recommendations for improvements in handling practices described in the recently published code of practice.
8. (Sub-objective 7) Use the findings to develop detailed knowledge and understanding of the physiological processes involved in stress responses in lobsters which can be used by processing companies and fishers to devise improved methods of post-harvest handling and transport.
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