73 results

SCRC: PhD 6.08 Molecular assessment of spawning cues in temperate abalone

Project number: 2010-727
Project Status:
Completed
Budget expenditure: $0.00
Principal Investigator: James O. Harris
Organisation: Flinders University
Project start/end date: 30 Jun 2010 - 29 Jun 2013
Contact:
FRDC

Need

The spawning of cultured abalone for Australia’s expanding abalone industry is a ‘numbers game’ with hatcheries required to mature and induce more individuals than needed as gamete release is not under full control. While this is acceptable for commercial production the advent of selective breeding programs (SBPs) emerging across the industry means that increased spawning efficiency is crucial to provide for the spawning of highly valuable selected individuals on cue ensuring that the greatest genetic gains in production traits are realised. Greater control over spawning is key to ensuring maximum efficiency in the implementation of SBPs and also in producing the highly marketable interspecies hybrid between Haliotis rubra and H. laevigata.

The aim of this project is to unravel the signalling cues that regulate spawning and/or maturation processes in abalone. This will allow on farm interventional strategies to be developed. Peptide hormones are a complex set of messenger molecules controlling a wide diversity of regulatory functions including a multitude of physiological processes and behaviours within an organism. The latest mass spectrometry methods will enable the detection, identification and characterisation of these important signalling molecules. While there is currently a limited genetic resource available for Haliotis species in the absence of a reference genome, molecular genetic approaches will also be utilised alongside the peptide discovery process to address this shortfall in knowledge. By undertaking sampling at variable time points during the gonad conditioning and spawning process it will provide valuable insight into which of the peptides are involved in facilitating spawning cues. This is likely to result in the ability to develop external stimuli and/or methods that will enable spawning of temperate abalone on cue.

SCRC: PhD 6.06 – Capturing and maintaining genetic variation when initiating selective breeding programs for aquaculture

Project number: 2010-725
Project Status:
Completed
Budget expenditure: $0.00
Principal Investigator: Nicholas A. Robinson
Organisation: Flinders University
Project start/end date: 31 Mar 2010 - 31 Mar 2013
Contact:
FRDC

Need

Industry need:
The following projects have stated the need for establishing sustainable selective breeding programs;
• Development of a genetic management and improvement strategy for temperate marine finfish (2008/723).
• Scope and economic analysis of options for a nationally unified breeding program for abalone aquaculture (2008/722).
• Development of a genetic management strategy for cultured Barramundi (2008/758).

Final report

ISBN: 978-1-925982-92-3
Author: Shannon Loughnan
Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

Final Report • 2013-04-01 • 4.65 MB
2010-725-DLD-PhD.pdf

Summary

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market, however, before selective breeding programs can begin, it is important to assess the levels of genetic diversity and relatedness of current captive broodstock populations.

Mass spawning hatchery practices using small broodstock populations, in addition to the cannibalistic nature of some fish species, contribute to a reduction of genetic diversity from parent to offspring and throughout the juvenile grow-out stages. This is of concern when establishing a selective breeding program for such species because the genetic diversity that is captured in the start-up and initial generations of the program is the basic ingredient for future genetic improvement. The aim of this thesis was to examine methods for capturing and conserving genetic diversity in mass spawning Barramundi (Lates calcarifer), when constructing a base population for a long-term selective breeding program for the species.

The results suggest that a mass spawning group of at least 30 Barramundi individuals is required to achieve a high participation rate of breeders and to limit the loss of genetic variation transferred to the offspring. Options for selecting candidates to compose a founding population were explored, and recommendations made to promote the longevity and impact of a selective breeding program for Barramundi.

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SCRC: SCRC Honours Scholarship H4.10 - Impact of fish oil replacement on the expression of antioxidant genes and genes involved in the synthesis of highly unsaturated omega-3 fatty acids in YTK liver and muscle tissue. (Dr Kathy Schuller; Student Nathan Rout-Pitt)

Project number: 2009-782
Project Status:
Completed
Budget expenditure: $0.00
Principal Investigator: Kathy Schuller
Organisation: Flinders University
Project start/end date: 20 Dec 2009 - 20 Dec 2010
Contact:
FRDC

Final report

ISBN: 978-1-925982-74-9
Author: Nathan Rout-Pitt
Final Report • 2010-11-09 • 902.26 KB
2009-782-DLD-Hons.pdf

Summary

Fish flesh contains high levels of omega-3 long chain polyunsaturated fatty acids (n-3 LCPUFA) such as eicosapentaenoic (EPA) and docosahexaenoic acid (DHA) are beneficial for human health to help brain and neural development, decrease the risk of cardiovascular disease and help various other aspects of human lives. EPA and DHA can be synthesised via the LCPUFA synthesis pathway in fish from α-linolenic acid (ALA) which is abundant is some plant oils which are totally devoid of n-3 LCPUFA. Captured fish are currently used as a source of fish oil to feed fish in aquaculture, but capture fisheries are currently at their limit and using captured fish stocks as aquaculture feed is unsustainable and therefore alternative lipid sources to replace fish oil are required to allow the aquaculture industry to continue growing.

This research investigated dietary fish oil replacement with plant or terrestrial animal oils and its impact on the seafood product quality of Yellowtail Kingfish (Seriola lalandi), an important aquaculture species in South Australia. It investigated how this replacement affected the expression of the fatty acid synthesis genes ∆6 fatty acyl desaturase (∆6 fads) and fatty acyl elongase (elovl), and the antioxidant genes peroxiredoxin 1 and 4 (Prx1 and 4) and glutathione peroxidase 1 and 4 (GPx1 and 4).
It was found that fish oil substitution with canola oil and poultry oil did not significantly change the expression of the fads or elovl genes. The antioxidant genes also showed no significant changes in gene expression except for GPx1 which showed an increased expression in fish fed the canola oil-based diet compared to fish fed the fish oil-based diet. Based on these results, and the results of the hepatosomatic indices, it is proposed that GPx1 expression may be up-regulated in fish fed the canola oil-based diet due to increased immune system activity as a result of the relatively high concentration of omega-6 fatty acids in their flesh.

Final Report • 2010-11-09 • 902.26 KB
2009-782-DLD-Hons.pdf

Summary

Fish flesh contains high levels of omega-3 long chain polyunsaturated fatty acids (n-3 LCPUFA) such as eicosapentaenoic (EPA) and docosahexaenoic acid (DHA) are beneficial for human health to help brain and neural development, decrease the risk of cardiovascular disease and help various other aspects of human lives. EPA and DHA can be synthesised via the LCPUFA synthesis pathway in fish from α-linolenic acid (ALA) which is abundant is some plant oils which are totally devoid of n-3 LCPUFA. Captured fish are currently used as a source of fish oil to feed fish in aquaculture, but capture fisheries are currently at their limit and using captured fish stocks as aquaculture feed is unsustainable and therefore alternative lipid sources to replace fish oil are required to allow the aquaculture industry to continue growing.

This research investigated dietary fish oil replacement with plant or terrestrial animal oils and its impact on the seafood product quality of Yellowtail Kingfish (Seriola lalandi), an important aquaculture species in South Australia. It investigated how this replacement affected the expression of the fatty acid synthesis genes ∆6 fatty acyl desaturase (∆6 fads) and fatty acyl elongase (elovl), and the antioxidant genes peroxiredoxin 1 and 4 (Prx1 and 4) and glutathione peroxidase 1 and 4 (GPx1 and 4).
It was found that fish oil substitution with canola oil and poultry oil did not significantly change the expression of the fads or elovl genes. The antioxidant genes also showed no significant changes in gene expression except for GPx1 which showed an increased expression in fish fed the canola oil-based diet compared to fish fed the fish oil-based diet. Based on these results, and the results of the hepatosomatic indices, it is proposed that GPx1 expression may be up-regulated in fish fed the canola oil-based diet due to increased immune system activity as a result of the relatively high concentration of omega-6 fatty acids in their flesh.

Final Report • 2010-11-09 • 902.26 KB
2009-782-DLD-Hons.pdf

Summary

Fish flesh contains high levels of omega-3 long chain polyunsaturated fatty acids (n-3 LCPUFA) such as eicosapentaenoic (EPA) and docosahexaenoic acid (DHA) are beneficial for human health to help brain and neural development, decrease the risk of cardiovascular disease and help various other aspects of human lives. EPA and DHA can be synthesised via the LCPUFA synthesis pathway in fish from α-linolenic acid (ALA) which is abundant is some plant oils which are totally devoid of n-3 LCPUFA. Captured fish are currently used as a source of fish oil to feed fish in aquaculture, but capture fisheries are currently at their limit and using captured fish stocks as aquaculture feed is unsustainable and therefore alternative lipid sources to replace fish oil are required to allow the aquaculture industry to continue growing.

This research investigated dietary fish oil replacement with plant or terrestrial animal oils and its impact on the seafood product quality of Yellowtail Kingfish (Seriola lalandi), an important aquaculture species in South Australia. It investigated how this replacement affected the expression of the fatty acid synthesis genes ∆6 fatty acyl desaturase (∆6 fads) and fatty acyl elongase (elovl), and the antioxidant genes peroxiredoxin 1 and 4 (Prx1 and 4) and glutathione peroxidase 1 and 4 (GPx1 and 4).
It was found that fish oil substitution with canola oil and poultry oil did not significantly change the expression of the fads or elovl genes. The antioxidant genes also showed no significant changes in gene expression except for GPx1 which showed an increased expression in fish fed the canola oil-based diet compared to fish fed the fish oil-based diet. Based on these results, and the results of the hepatosomatic indices, it is proposed that GPx1 expression may be up-regulated in fish fed the canola oil-based diet due to increased immune system activity as a result of the relatively high concentration of omega-6 fatty acids in their flesh.

Final Report • 2010-11-09 • 902.26 KB
2009-782-DLD-Hons.pdf

Summary

Fish flesh contains high levels of omega-3 long chain polyunsaturated fatty acids (n-3 LCPUFA) such as eicosapentaenoic (EPA) and docosahexaenoic acid (DHA) are beneficial for human health to help brain and neural development, decrease the risk of cardiovascular disease and help various other aspects of human lives. EPA and DHA can be synthesised via the LCPUFA synthesis pathway in fish from α-linolenic acid (ALA) which is abundant is some plant oils which are totally devoid of n-3 LCPUFA. Captured fish are currently used as a source of fish oil to feed fish in aquaculture, but capture fisheries are currently at their limit and using captured fish stocks as aquaculture feed is unsustainable and therefore alternative lipid sources to replace fish oil are required to allow the aquaculture industry to continue growing.

This research investigated dietary fish oil replacement with plant or terrestrial animal oils and its impact on the seafood product quality of Yellowtail Kingfish (Seriola lalandi), an important aquaculture species in South Australia. It investigated how this replacement affected the expression of the fatty acid synthesis genes ∆6 fatty acyl desaturase (∆6 fads) and fatty acyl elongase (elovl), and the antioxidant genes peroxiredoxin 1 and 4 (Prx1 and 4) and glutathione peroxidase 1 and 4 (GPx1 and 4).
It was found that fish oil substitution with canola oil and poultry oil did not significantly change the expression of the fads or elovl genes. The antioxidant genes also showed no significant changes in gene expression except for GPx1 which showed an increased expression in fish fed the canola oil-based diet compared to fish fed the fish oil-based diet. Based on these results, and the results of the hepatosomatic indices, it is proposed that GPx1 expression may be up-regulated in fish fed the canola oil-based diet due to increased immune system activity as a result of the relatively high concentration of omega-6 fatty acids in their flesh.

Final Report • 2010-11-09 • 902.26 KB
2009-782-DLD-Hons.pdf

Summary

Fish flesh contains high levels of omega-3 long chain polyunsaturated fatty acids (n-3 LCPUFA) such as eicosapentaenoic (EPA) and docosahexaenoic acid (DHA) are beneficial for human health to help brain and neural development, decrease the risk of cardiovascular disease and help various other aspects of human lives. EPA and DHA can be synthesised via the LCPUFA synthesis pathway in fish from α-linolenic acid (ALA) which is abundant is some plant oils which are totally devoid of n-3 LCPUFA. Captured fish are currently used as a source of fish oil to feed fish in aquaculture, but capture fisheries are currently at their limit and using captured fish stocks as aquaculture feed is unsustainable and therefore alternative lipid sources to replace fish oil are required to allow the aquaculture industry to continue growing.

This research investigated dietary fish oil replacement with plant or terrestrial animal oils and its impact on the seafood product quality of Yellowtail Kingfish (Seriola lalandi), an important aquaculture species in South Australia. It investigated how this replacement affected the expression of the fatty acid synthesis genes ∆6 fatty acyl desaturase (∆6 fads) and fatty acyl elongase (elovl), and the antioxidant genes peroxiredoxin 1 and 4 (Prx1 and 4) and glutathione peroxidase 1 and 4 (GPx1 and 4).
It was found that fish oil substitution with canola oil and poultry oil did not significantly change the expression of the fads or elovl genes. The antioxidant genes also showed no significant changes in gene expression except for GPx1 which showed an increased expression in fish fed the canola oil-based diet compared to fish fed the fish oil-based diet. Based on these results, and the results of the hepatosomatic indices, it is proposed that GPx1 expression may be up-regulated in fish fed the canola oil-based diet due to increased immune system activity as a result of the relatively high concentration of omega-6 fatty acids in their flesh.

Final Report • 2010-11-09 • 902.26 KB
2009-782-DLD-Hons.pdf

Summary

Fish flesh contains high levels of omega-3 long chain polyunsaturated fatty acids (n-3 LCPUFA) such as eicosapentaenoic (EPA) and docosahexaenoic acid (DHA) are beneficial for human health to help brain and neural development, decrease the risk of cardiovascular disease and help various other aspects of human lives. EPA and DHA can be synthesised via the LCPUFA synthesis pathway in fish from α-linolenic acid (ALA) which is abundant is some plant oils which are totally devoid of n-3 LCPUFA. Captured fish are currently used as a source of fish oil to feed fish in aquaculture, but capture fisheries are currently at their limit and using captured fish stocks as aquaculture feed is unsustainable and therefore alternative lipid sources to replace fish oil are required to allow the aquaculture industry to continue growing.

This research investigated dietary fish oil replacement with plant or terrestrial animal oils and its impact on the seafood product quality of Yellowtail Kingfish (Seriola lalandi), an important aquaculture species in South Australia. It investigated how this replacement affected the expression of the fatty acid synthesis genes ∆6 fatty acyl desaturase (∆6 fads) and fatty acyl elongase (elovl), and the antioxidant genes peroxiredoxin 1 and 4 (Prx1 and 4) and glutathione peroxidase 1 and 4 (GPx1 and 4).
It was found that fish oil substitution with canola oil and poultry oil did not significantly change the expression of the fads or elovl genes. The antioxidant genes also showed no significant changes in gene expression except for GPx1 which showed an increased expression in fish fed the canola oil-based diet compared to fish fed the fish oil-based diet. Based on these results, and the results of the hepatosomatic indices, it is proposed that GPx1 expression may be up-regulated in fish fed the canola oil-based diet due to increased immune system activity as a result of the relatively high concentration of omega-6 fatty acids in their flesh.

Final Report • 2010-11-09 • 902.26 KB
2009-782-DLD-Hons.pdf

Summary

Fish flesh contains high levels of omega-3 long chain polyunsaturated fatty acids (n-3 LCPUFA) such as eicosapentaenoic (EPA) and docosahexaenoic acid (DHA) are beneficial for human health to help brain and neural development, decrease the risk of cardiovascular disease and help various other aspects of human lives. EPA and DHA can be synthesised via the LCPUFA synthesis pathway in fish from α-linolenic acid (ALA) which is abundant is some plant oils which are totally devoid of n-3 LCPUFA. Captured fish are currently used as a source of fish oil to feed fish in aquaculture, but capture fisheries are currently at their limit and using captured fish stocks as aquaculture feed is unsustainable and therefore alternative lipid sources to replace fish oil are required to allow the aquaculture industry to continue growing.

This research investigated dietary fish oil replacement with plant or terrestrial animal oils and its impact on the seafood product quality of Yellowtail Kingfish (Seriola lalandi), an important aquaculture species in South Australia. It investigated how this replacement affected the expression of the fatty acid synthesis genes ∆6 fatty acyl desaturase (∆6 fads) and fatty acyl elongase (elovl), and the antioxidant genes peroxiredoxin 1 and 4 (Prx1 and 4) and glutathione peroxidase 1 and 4 (GPx1 and 4).
It was found that fish oil substitution with canola oil and poultry oil did not significantly change the expression of the fads or elovl genes. The antioxidant genes also showed no significant changes in gene expression except for GPx1 which showed an increased expression in fish fed the canola oil-based diet compared to fish fed the fish oil-based diet. Based on these results, and the results of the hepatosomatic indices, it is proposed that GPx1 expression may be up-regulated in fish fed the canola oil-based diet due to increased immune system activity as a result of the relatively high concentration of omega-6 fatty acids in their flesh.

Final Report • 2010-11-09 • 902.26 KB
2009-782-DLD-Hons.pdf

Summary

Fish flesh contains high levels of omega-3 long chain polyunsaturated fatty acids (n-3 LCPUFA) such as eicosapentaenoic (EPA) and docosahexaenoic acid (DHA) are beneficial for human health to help brain and neural development, decrease the risk of cardiovascular disease and help various other aspects of human lives. EPA and DHA can be synthesised via the LCPUFA synthesis pathway in fish from α-linolenic acid (ALA) which is abundant is some plant oils which are totally devoid of n-3 LCPUFA. Captured fish are currently used as a source of fish oil to feed fish in aquaculture, but capture fisheries are currently at their limit and using captured fish stocks as aquaculture feed is unsustainable and therefore alternative lipid sources to replace fish oil are required to allow the aquaculture industry to continue growing.

This research investigated dietary fish oil replacement with plant or terrestrial animal oils and its impact on the seafood product quality of Yellowtail Kingfish (Seriola lalandi), an important aquaculture species in South Australia. It investigated how this replacement affected the expression of the fatty acid synthesis genes ∆6 fatty acyl desaturase (∆6 fads) and fatty acyl elongase (elovl), and the antioxidant genes peroxiredoxin 1 and 4 (Prx1 and 4) and glutathione peroxidase 1 and 4 (GPx1 and 4).
It was found that fish oil substitution with canola oil and poultry oil did not significantly change the expression of the fads or elovl genes. The antioxidant genes also showed no significant changes in gene expression except for GPx1 which showed an increased expression in fish fed the canola oil-based diet compared to fish fed the fish oil-based diet. Based on these results, and the results of the hepatosomatic indices, it is proposed that GPx1 expression may be up-regulated in fish fed the canola oil-based diet due to increased immune system activity as a result of the relatively high concentration of omega-6 fatty acids in their flesh.

Final Report • 2010-11-09 • 902.26 KB
2009-782-DLD-Hons.pdf

Summary

Fish flesh contains high levels of omega-3 long chain polyunsaturated fatty acids (n-3 LCPUFA) such as eicosapentaenoic (EPA) and docosahexaenoic acid (DHA) are beneficial for human health to help brain and neural development, decrease the risk of cardiovascular disease and help various other aspects of human lives. EPA and DHA can be synthesised via the LCPUFA synthesis pathway in fish from α-linolenic acid (ALA) which is abundant is some plant oils which are totally devoid of n-3 LCPUFA. Captured fish are currently used as a source of fish oil to feed fish in aquaculture, but capture fisheries are currently at their limit and using captured fish stocks as aquaculture feed is unsustainable and therefore alternative lipid sources to replace fish oil are required to allow the aquaculture industry to continue growing.

This research investigated dietary fish oil replacement with plant or terrestrial animal oils and its impact on the seafood product quality of Yellowtail Kingfish (Seriola lalandi), an important aquaculture species in South Australia. It investigated how this replacement affected the expression of the fatty acid synthesis genes ∆6 fatty acyl desaturase (∆6 fads) and fatty acyl elongase (elovl), and the antioxidant genes peroxiredoxin 1 and 4 (Prx1 and 4) and glutathione peroxidase 1 and 4 (GPx1 and 4).
It was found that fish oil substitution with canola oil and poultry oil did not significantly change the expression of the fads or elovl genes. The antioxidant genes also showed no significant changes in gene expression except for GPx1 which showed an increased expression in fish fed the canola oil-based diet compared to fish fed the fish oil-based diet. Based on these results, and the results of the hepatosomatic indices, it is proposed that GPx1 expression may be up-regulated in fish fed the canola oil-based diet due to increased immune system activity as a result of the relatively high concentration of omega-6 fatty acids in their flesh.

Final Report • 2010-11-09 • 902.26 KB
2009-782-DLD-Hons.pdf

Summary

Fish flesh contains high levels of omega-3 long chain polyunsaturated fatty acids (n-3 LCPUFA) such as eicosapentaenoic (EPA) and docosahexaenoic acid (DHA) are beneficial for human health to help brain and neural development, decrease the risk of cardiovascular disease and help various other aspects of human lives. EPA and DHA can be synthesised via the LCPUFA synthesis pathway in fish from α-linolenic acid (ALA) which is abundant is some plant oils which are totally devoid of n-3 LCPUFA. Captured fish are currently used as a source of fish oil to feed fish in aquaculture, but capture fisheries are currently at their limit and using captured fish stocks as aquaculture feed is unsustainable and therefore alternative lipid sources to replace fish oil are required to allow the aquaculture industry to continue growing.

This research investigated dietary fish oil replacement with plant or terrestrial animal oils and its impact on the seafood product quality of Yellowtail Kingfish (Seriola lalandi), an important aquaculture species in South Australia. It investigated how this replacement affected the expression of the fatty acid synthesis genes ∆6 fatty acyl desaturase (∆6 fads) and fatty acyl elongase (elovl), and the antioxidant genes peroxiredoxin 1 and 4 (Prx1 and 4) and glutathione peroxidase 1 and 4 (GPx1 and 4).
It was found that fish oil substitution with canola oil and poultry oil did not significantly change the expression of the fads or elovl genes. The antioxidant genes also showed no significant changes in gene expression except for GPx1 which showed an increased expression in fish fed the canola oil-based diet compared to fish fed the fish oil-based diet. Based on these results, and the results of the hepatosomatic indices, it is proposed that GPx1 expression may be up-regulated in fish fed the canola oil-based diet due to increased immune system activity as a result of the relatively high concentration of omega-6 fatty acids in their flesh.

Final Report • 2010-11-09 • 902.26 KB
2009-782-DLD-Hons.pdf

Summary

Fish flesh contains high levels of omega-3 long chain polyunsaturated fatty acids (n-3 LCPUFA) such as eicosapentaenoic (EPA) and docosahexaenoic acid (DHA) are beneficial for human health to help brain and neural development, decrease the risk of cardiovascular disease and help various other aspects of human lives. EPA and DHA can be synthesised via the LCPUFA synthesis pathway in fish from α-linolenic acid (ALA) which is abundant is some plant oils which are totally devoid of n-3 LCPUFA. Captured fish are currently used as a source of fish oil to feed fish in aquaculture, but capture fisheries are currently at their limit and using captured fish stocks as aquaculture feed is unsustainable and therefore alternative lipid sources to replace fish oil are required to allow the aquaculture industry to continue growing.

This research investigated dietary fish oil replacement with plant or terrestrial animal oils and its impact on the seafood product quality of Yellowtail Kingfish (Seriola lalandi), an important aquaculture species in South Australia. It investigated how this replacement affected the expression of the fatty acid synthesis genes ∆6 fatty acyl desaturase (∆6 fads) and fatty acyl elongase (elovl), and the antioxidant genes peroxiredoxin 1 and 4 (Prx1 and 4) and glutathione peroxidase 1 and 4 (GPx1 and 4).
It was found that fish oil substitution with canola oil and poultry oil did not significantly change the expression of the fads or elovl genes. The antioxidant genes also showed no significant changes in gene expression except for GPx1 which showed an increased expression in fish fed the canola oil-based diet compared to fish fed the fish oil-based diet. Based on these results, and the results of the hepatosomatic indices, it is proposed that GPx1 expression may be up-regulated in fish fed the canola oil-based diet due to increased immune system activity as a result of the relatively high concentration of omega-6 fatty acids in their flesh.

Final Report • 2010-11-09 • 902.26 KB
2009-782-DLD-Hons.pdf

Summary

Fish flesh contains high levels of omega-3 long chain polyunsaturated fatty acids (n-3 LCPUFA) such as eicosapentaenoic (EPA) and docosahexaenoic acid (DHA) are beneficial for human health to help brain and neural development, decrease the risk of cardiovascular disease and help various other aspects of human lives. EPA and DHA can be synthesised via the LCPUFA synthesis pathway in fish from α-linolenic acid (ALA) which is abundant is some plant oils which are totally devoid of n-3 LCPUFA. Captured fish are currently used as a source of fish oil to feed fish in aquaculture, but capture fisheries are currently at their limit and using captured fish stocks as aquaculture feed is unsustainable and therefore alternative lipid sources to replace fish oil are required to allow the aquaculture industry to continue growing.

This research investigated dietary fish oil replacement with plant or terrestrial animal oils and its impact on the seafood product quality of Yellowtail Kingfish (Seriola lalandi), an important aquaculture species in South Australia. It investigated how this replacement affected the expression of the fatty acid synthesis genes ∆6 fatty acyl desaturase (∆6 fads) and fatty acyl elongase (elovl), and the antioxidant genes peroxiredoxin 1 and 4 (Prx1 and 4) and glutathione peroxidase 1 and 4 (GPx1 and 4).
It was found that fish oil substitution with canola oil and poultry oil did not significantly change the expression of the fads or elovl genes. The antioxidant genes also showed no significant changes in gene expression except for GPx1 which showed an increased expression in fish fed the canola oil-based diet compared to fish fed the fish oil-based diet. Based on these results, and the results of the hepatosomatic indices, it is proposed that GPx1 expression may be up-regulated in fish fed the canola oil-based diet due to increased immune system activity as a result of the relatively high concentration of omega-6 fatty acids in their flesh.

Final Report • 2010-11-09 • 902.26 KB
2009-782-DLD-Hons.pdf

Summary

Fish flesh contains high levels of omega-3 long chain polyunsaturated fatty acids (n-3 LCPUFA) such as eicosapentaenoic (EPA) and docosahexaenoic acid (DHA) are beneficial for human health to help brain and neural development, decrease the risk of cardiovascular disease and help various other aspects of human lives. EPA and DHA can be synthesised via the LCPUFA synthesis pathway in fish from α-linolenic acid (ALA) which is abundant is some plant oils which are totally devoid of n-3 LCPUFA. Captured fish are currently used as a source of fish oil to feed fish in aquaculture, but capture fisheries are currently at their limit and using captured fish stocks as aquaculture feed is unsustainable and therefore alternative lipid sources to replace fish oil are required to allow the aquaculture industry to continue growing.

This research investigated dietary fish oil replacement with plant or terrestrial animal oils and its impact on the seafood product quality of Yellowtail Kingfish (Seriola lalandi), an important aquaculture species in South Australia. It investigated how this replacement affected the expression of the fatty acid synthesis genes ∆6 fatty acyl desaturase (∆6 fads) and fatty acyl elongase (elovl), and the antioxidant genes peroxiredoxin 1 and 4 (Prx1 and 4) and glutathione peroxidase 1 and 4 (GPx1 and 4).
It was found that fish oil substitution with canola oil and poultry oil did not significantly change the expression of the fads or elovl genes. The antioxidant genes also showed no significant changes in gene expression except for GPx1 which showed an increased expression in fish fed the canola oil-based diet compared to fish fed the fish oil-based diet. Based on these results, and the results of the hepatosomatic indices, it is proposed that GPx1 expression may be up-regulated in fish fed the canola oil-based diet due to increased immune system activity as a result of the relatively high concentration of omega-6 fatty acids in their flesh.

Final Report • 2010-11-09 • 902.26 KB
2009-782-DLD-Hons.pdf

Summary

Fish flesh contains high levels of omega-3 long chain polyunsaturated fatty acids (n-3 LCPUFA) such as eicosapentaenoic (EPA) and docosahexaenoic acid (DHA) are beneficial for human health to help brain and neural development, decrease the risk of cardiovascular disease and help various other aspects of human lives. EPA and DHA can be synthesised via the LCPUFA synthesis pathway in fish from α-linolenic acid (ALA) which is abundant is some plant oils which are totally devoid of n-3 LCPUFA. Captured fish are currently used as a source of fish oil to feed fish in aquaculture, but capture fisheries are currently at their limit and using captured fish stocks as aquaculture feed is unsustainable and therefore alternative lipid sources to replace fish oil are required to allow the aquaculture industry to continue growing.

This research investigated dietary fish oil replacement with plant or terrestrial animal oils and its impact on the seafood product quality of Yellowtail Kingfish (Seriola lalandi), an important aquaculture species in South Australia. It investigated how this replacement affected the expression of the fatty acid synthesis genes ∆6 fatty acyl desaturase (∆6 fads) and fatty acyl elongase (elovl), and the antioxidant genes peroxiredoxin 1 and 4 (Prx1 and 4) and glutathione peroxidase 1 and 4 (GPx1 and 4).
It was found that fish oil substitution with canola oil and poultry oil did not significantly change the expression of the fads or elovl genes. The antioxidant genes also showed no significant changes in gene expression except for GPx1 which showed an increased expression in fish fed the canola oil-based diet compared to fish fed the fish oil-based diet. Based on these results, and the results of the hepatosomatic indices, it is proposed that GPx1 expression may be up-regulated in fish fed the canola oil-based diet due to increased immune system activity as a result of the relatively high concentration of omega-6 fatty acids in their flesh.

Final Report • 2010-11-09 • 902.26 KB
2009-782-DLD-Hons.pdf

Summary

Fish flesh contains high levels of omega-3 long chain polyunsaturated fatty acids (n-3 LCPUFA) such as eicosapentaenoic (EPA) and docosahexaenoic acid (DHA) are beneficial for human health to help brain and neural development, decrease the risk of cardiovascular disease and help various other aspects of human lives. EPA and DHA can be synthesised via the LCPUFA synthesis pathway in fish from α-linolenic acid (ALA) which is abundant is some plant oils which are totally devoid of n-3 LCPUFA. Captured fish are currently used as a source of fish oil to feed fish in aquaculture, but capture fisheries are currently at their limit and using captured fish stocks as aquaculture feed is unsustainable and therefore alternative lipid sources to replace fish oil are required to allow the aquaculture industry to continue growing.

This research investigated dietary fish oil replacement with plant or terrestrial animal oils and its impact on the seafood product quality of Yellowtail Kingfish (Seriola lalandi), an important aquaculture species in South Australia. It investigated how this replacement affected the expression of the fatty acid synthesis genes ∆6 fatty acyl desaturase (∆6 fads) and fatty acyl elongase (elovl), and the antioxidant genes peroxiredoxin 1 and 4 (Prx1 and 4) and glutathione peroxidase 1 and 4 (GPx1 and 4).
It was found that fish oil substitution with canola oil and poultry oil did not significantly change the expression of the fads or elovl genes. The antioxidant genes also showed no significant changes in gene expression except for GPx1 which showed an increased expression in fish fed the canola oil-based diet compared to fish fed the fish oil-based diet. Based on these results, and the results of the hepatosomatic indices, it is proposed that GPx1 expression may be up-regulated in fish fed the canola oil-based diet due to increased immune system activity as a result of the relatively high concentration of omega-6 fatty acids in their flesh.

Final Report • 2010-11-09 • 902.26 KB
2009-782-DLD-Hons.pdf

Summary

Fish flesh contains high levels of omega-3 long chain polyunsaturated fatty acids (n-3 LCPUFA) such as eicosapentaenoic (EPA) and docosahexaenoic acid (DHA) are beneficial for human health to help brain and neural development, decrease the risk of cardiovascular disease and help various other aspects of human lives. EPA and DHA can be synthesised via the LCPUFA synthesis pathway in fish from α-linolenic acid (ALA) which is abundant is some plant oils which are totally devoid of n-3 LCPUFA. Captured fish are currently used as a source of fish oil to feed fish in aquaculture, but capture fisheries are currently at their limit and using captured fish stocks as aquaculture feed is unsustainable and therefore alternative lipid sources to replace fish oil are required to allow the aquaculture industry to continue growing.

This research investigated dietary fish oil replacement with plant or terrestrial animal oils and its impact on the seafood product quality of Yellowtail Kingfish (Seriola lalandi), an important aquaculture species in South Australia. It investigated how this replacement affected the expression of the fatty acid synthesis genes ∆6 fatty acyl desaturase (∆6 fads) and fatty acyl elongase (elovl), and the antioxidant genes peroxiredoxin 1 and 4 (Prx1 and 4) and glutathione peroxidase 1 and 4 (GPx1 and 4).
It was found that fish oil substitution with canola oil and poultry oil did not significantly change the expression of the fads or elovl genes. The antioxidant genes also showed no significant changes in gene expression except for GPx1 which showed an increased expression in fish fed the canola oil-based diet compared to fish fed the fish oil-based diet. Based on these results, and the results of the hepatosomatic indices, it is proposed that GPx1 expression may be up-regulated in fish fed the canola oil-based diet due to increased immune system activity as a result of the relatively high concentration of omega-6 fatty acids in their flesh.

Final Report • 2010-11-09 • 902.26 KB
2009-782-DLD-Hons.pdf

Summary

Fish flesh contains high levels of omega-3 long chain polyunsaturated fatty acids (n-3 LCPUFA) such as eicosapentaenoic (EPA) and docosahexaenoic acid (DHA) are beneficial for human health to help brain and neural development, decrease the risk of cardiovascular disease and help various other aspects of human lives. EPA and DHA can be synthesised via the LCPUFA synthesis pathway in fish from α-linolenic acid (ALA) which is abundant is some plant oils which are totally devoid of n-3 LCPUFA. Captured fish are currently used as a source of fish oil to feed fish in aquaculture, but capture fisheries are currently at their limit and using captured fish stocks as aquaculture feed is unsustainable and therefore alternative lipid sources to replace fish oil are required to allow the aquaculture industry to continue growing.

This research investigated dietary fish oil replacement with plant or terrestrial animal oils and its impact on the seafood product quality of Yellowtail Kingfish (Seriola lalandi), an important aquaculture species in South Australia. It investigated how this replacement affected the expression of the fatty acid synthesis genes ∆6 fatty acyl desaturase (∆6 fads) and fatty acyl elongase (elovl), and the antioxidant genes peroxiredoxin 1 and 4 (Prx1 and 4) and glutathione peroxidase 1 and 4 (GPx1 and 4).
It was found that fish oil substitution with canola oil and poultry oil did not significantly change the expression of the fads or elovl genes. The antioxidant genes also showed no significant changes in gene expression except for GPx1 which showed an increased expression in fish fed the canola oil-based diet compared to fish fed the fish oil-based diet. Based on these results, and the results of the hepatosomatic indices, it is proposed that GPx1 expression may be up-regulated in fish fed the canola oil-based diet due to increased immune system activity as a result of the relatively high concentration of omega-6 fatty acids in their flesh.

SCRC: SCRC Honours Scholarship H4.6 Effect of temperature and plant protein inclusion on the growth factor gene expression in Yellowtail Kingfish (Jian Qin; Student Geoffrey Collins)

Project number: 2009-780
Project Status:
Completed
Budget expenditure: $0.00
Principal Investigator: Jian Qin
Organisation: Flinders University
Project start/end date: 20 Dec 2009 - 20 Dec 2010
Contact:
FRDC

Final report

ISBN: 978-1-925982-79-4
Author: Geoffrey M Collins
Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

SCRC: SCRC Honours Scholarship H4.5 Improved Yellowtail Kingfish Growth Response, Flesh Quality and PUFA content through Biorefinery Microalgae LC-PUFA Aqua-Feed Additives (Prof Wei Zhang; Student Kopano Maso Machailo)

Project number: 2009-779
Project Status:
Completed
Budget expenditure: $0.00
Principal Investigator: Wei Zhang
Organisation: Flinders University
Project start/end date: 20 Dec 2009 - 20 Dec 2010
Contact:
FRDC

Final report

ISBN: 978-1-925982-43-5
Author: Kopano Mosa Machailo
Final Report • 2010-11-03 • 1.44 MB
2009-779-DLD-Hons.pdf

Summary

Yellowtail Kingfish is an emerging aquaculture species, and there is an increase in the demand for the fish, both for recreational fishing and for export purposes. This has led to the growth of the aquaculture industry that specialises in culture of yellowtail kingfish in South Australia. Currently very little is known about the physiological properties of yellowtail kingfish, and most data that has been generated on the fish has primarily been in Japan, where most of the scientific articles are written in Japanese.

There is currently very limited supply of microalgae in Australia for use in commercial applications, and development is underway to encourage growth of the microalgae industry with a specific focus on developing a biorefinery system. One of the main aspects of the biorefinery system will be to use waste generated by the production of biofuels in other industries which will provide value addition to the waste products from the production of biodiesel.

Final Report • 2010-11-03 • 1.44 MB
2009-779-DLD-Hons.pdf

Summary

Yellowtail Kingfish is an emerging aquaculture species, and there is an increase in the demand for the fish, both for recreational fishing and for export purposes. This has led to the growth of the aquaculture industry that specialises in culture of yellowtail kingfish in South Australia. Currently very little is known about the physiological properties of yellowtail kingfish, and most data that has been generated on the fish has primarily been in Japan, where most of the scientific articles are written in Japanese.

There is currently very limited supply of microalgae in Australia for use in commercial applications, and development is underway to encourage growth of the microalgae industry with a specific focus on developing a biorefinery system. One of the main aspects of the biorefinery system will be to use waste generated by the production of biofuels in other industries which will provide value addition to the waste products from the production of biodiesel.

Final Report • 2010-11-03 • 1.44 MB
2009-779-DLD-Hons.pdf

Summary

Yellowtail Kingfish is an emerging aquaculture species, and there is an increase in the demand for the fish, both for recreational fishing and for export purposes. This has led to the growth of the aquaculture industry that specialises in culture of yellowtail kingfish in South Australia. Currently very little is known about the physiological properties of yellowtail kingfish, and most data that has been generated on the fish has primarily been in Japan, where most of the scientific articles are written in Japanese.

There is currently very limited supply of microalgae in Australia for use in commercial applications, and development is underway to encourage growth of the microalgae industry with a specific focus on developing a biorefinery system. One of the main aspects of the biorefinery system will be to use waste generated by the production of biofuels in other industries which will provide value addition to the waste products from the production of biodiesel.

Final Report • 2010-11-03 • 1.44 MB
2009-779-DLD-Hons.pdf

Summary

Yellowtail Kingfish is an emerging aquaculture species, and there is an increase in the demand for the fish, both for recreational fishing and for export purposes. This has led to the growth of the aquaculture industry that specialises in culture of yellowtail kingfish in South Australia. Currently very little is known about the physiological properties of yellowtail kingfish, and most data that has been generated on the fish has primarily been in Japan, where most of the scientific articles are written in Japanese.

There is currently very limited supply of microalgae in Australia for use in commercial applications, and development is underway to encourage growth of the microalgae industry with a specific focus on developing a biorefinery system. One of the main aspects of the biorefinery system will be to use waste generated by the production of biofuels in other industries which will provide value addition to the waste products from the production of biodiesel.

Final Report • 2010-11-03 • 1.44 MB
2009-779-DLD-Hons.pdf

Summary

Yellowtail Kingfish is an emerging aquaculture species, and there is an increase in the demand for the fish, both for recreational fishing and for export purposes. This has led to the growth of the aquaculture industry that specialises in culture of yellowtail kingfish in South Australia. Currently very little is known about the physiological properties of yellowtail kingfish, and most data that has been generated on the fish has primarily been in Japan, where most of the scientific articles are written in Japanese.

There is currently very limited supply of microalgae in Australia for use in commercial applications, and development is underway to encourage growth of the microalgae industry with a specific focus on developing a biorefinery system. One of the main aspects of the biorefinery system will be to use waste generated by the production of biofuels in other industries which will provide value addition to the waste products from the production of biodiesel.

Final Report • 2010-11-03 • 1.44 MB
2009-779-DLD-Hons.pdf

Summary

Yellowtail Kingfish is an emerging aquaculture species, and there is an increase in the demand for the fish, both for recreational fishing and for export purposes. This has led to the growth of the aquaculture industry that specialises in culture of yellowtail kingfish in South Australia. Currently very little is known about the physiological properties of yellowtail kingfish, and most data that has been generated on the fish has primarily been in Japan, where most of the scientific articles are written in Japanese.

There is currently very limited supply of microalgae in Australia for use in commercial applications, and development is underway to encourage growth of the microalgae industry with a specific focus on developing a biorefinery system. One of the main aspects of the biorefinery system will be to use waste generated by the production of biofuels in other industries which will provide value addition to the waste products from the production of biodiesel.

Final Report • 2010-11-03 • 1.44 MB
2009-779-DLD-Hons.pdf

Summary

Yellowtail Kingfish is an emerging aquaculture species, and there is an increase in the demand for the fish, both for recreational fishing and for export purposes. This has led to the growth of the aquaculture industry that specialises in culture of yellowtail kingfish in South Australia. Currently very little is known about the physiological properties of yellowtail kingfish, and most data that has been generated on the fish has primarily been in Japan, where most of the scientific articles are written in Japanese.

There is currently very limited supply of microalgae in Australia for use in commercial applications, and development is underway to encourage growth of the microalgae industry with a specific focus on developing a biorefinery system. One of the main aspects of the biorefinery system will be to use waste generated by the production of biofuels in other industries which will provide value addition to the waste products from the production of biodiesel.

Final Report • 2010-11-03 • 1.44 MB
2009-779-DLD-Hons.pdf

Summary

Yellowtail Kingfish is an emerging aquaculture species, and there is an increase in the demand for the fish, both for recreational fishing and for export purposes. This has led to the growth of the aquaculture industry that specialises in culture of yellowtail kingfish in South Australia. Currently very little is known about the physiological properties of yellowtail kingfish, and most data that has been generated on the fish has primarily been in Japan, where most of the scientific articles are written in Japanese.

There is currently very limited supply of microalgae in Australia for use in commercial applications, and development is underway to encourage growth of the microalgae industry with a specific focus on developing a biorefinery system. One of the main aspects of the biorefinery system will be to use waste generated by the production of biofuels in other industries which will provide value addition to the waste products from the production of biodiesel.

Final Report • 2010-11-03 • 1.44 MB
2009-779-DLD-Hons.pdf

Summary

Yellowtail Kingfish is an emerging aquaculture species, and there is an increase in the demand for the fish, both for recreational fishing and for export purposes. This has led to the growth of the aquaculture industry that specialises in culture of yellowtail kingfish in South Australia. Currently very little is known about the physiological properties of yellowtail kingfish, and most data that has been generated on the fish has primarily been in Japan, where most of the scientific articles are written in Japanese.

There is currently very limited supply of microalgae in Australia for use in commercial applications, and development is underway to encourage growth of the microalgae industry with a specific focus on developing a biorefinery system. One of the main aspects of the biorefinery system will be to use waste generated by the production of biofuels in other industries which will provide value addition to the waste products from the production of biodiesel.

Final Report • 2010-11-03 • 1.44 MB
2009-779-DLD-Hons.pdf

Summary

Yellowtail Kingfish is an emerging aquaculture species, and there is an increase in the demand for the fish, both for recreational fishing and for export purposes. This has led to the growth of the aquaculture industry that specialises in culture of yellowtail kingfish in South Australia. Currently very little is known about the physiological properties of yellowtail kingfish, and most data that has been generated on the fish has primarily been in Japan, where most of the scientific articles are written in Japanese.

There is currently very limited supply of microalgae in Australia for use in commercial applications, and development is underway to encourage growth of the microalgae industry with a specific focus on developing a biorefinery system. One of the main aspects of the biorefinery system will be to use waste generated by the production of biofuels in other industries which will provide value addition to the waste products from the production of biodiesel.

Final Report • 2010-11-03 • 1.44 MB
2009-779-DLD-Hons.pdf

Summary

Yellowtail Kingfish is an emerging aquaculture species, and there is an increase in the demand for the fish, both for recreational fishing and for export purposes. This has led to the growth of the aquaculture industry that specialises in culture of yellowtail kingfish in South Australia. Currently very little is known about the physiological properties of yellowtail kingfish, and most data that has been generated on the fish has primarily been in Japan, where most of the scientific articles are written in Japanese.

There is currently very limited supply of microalgae in Australia for use in commercial applications, and development is underway to encourage growth of the microalgae industry with a specific focus on developing a biorefinery system. One of the main aspects of the biorefinery system will be to use waste generated by the production of biofuels in other industries which will provide value addition to the waste products from the production of biodiesel.

Final Report • 2010-11-03 • 1.44 MB
2009-779-DLD-Hons.pdf

Summary

Yellowtail Kingfish is an emerging aquaculture species, and there is an increase in the demand for the fish, both for recreational fishing and for export purposes. This has led to the growth of the aquaculture industry that specialises in culture of yellowtail kingfish in South Australia. Currently very little is known about the physiological properties of yellowtail kingfish, and most data that has been generated on the fish has primarily been in Japan, where most of the scientific articles are written in Japanese.

There is currently very limited supply of microalgae in Australia for use in commercial applications, and development is underway to encourage growth of the microalgae industry with a specific focus on developing a biorefinery system. One of the main aspects of the biorefinery system will be to use waste generated by the production of biofuels in other industries which will provide value addition to the waste products from the production of biodiesel.

Final Report • 2010-11-03 • 1.44 MB
2009-779-DLD-Hons.pdf

Summary

Yellowtail Kingfish is an emerging aquaculture species, and there is an increase in the demand for the fish, both for recreational fishing and for export purposes. This has led to the growth of the aquaculture industry that specialises in culture of yellowtail kingfish in South Australia. Currently very little is known about the physiological properties of yellowtail kingfish, and most data that has been generated on the fish has primarily been in Japan, where most of the scientific articles are written in Japanese.

There is currently very limited supply of microalgae in Australia for use in commercial applications, and development is underway to encourage growth of the microalgae industry with a specific focus on developing a biorefinery system. One of the main aspects of the biorefinery system will be to use waste generated by the production of biofuels in other industries which will provide value addition to the waste products from the production of biodiesel.

Final Report • 2010-11-03 • 1.44 MB
2009-779-DLD-Hons.pdf

Summary

Yellowtail Kingfish is an emerging aquaculture species, and there is an increase in the demand for the fish, both for recreational fishing and for export purposes. This has led to the growth of the aquaculture industry that specialises in culture of yellowtail kingfish in South Australia. Currently very little is known about the physiological properties of yellowtail kingfish, and most data that has been generated on the fish has primarily been in Japan, where most of the scientific articles are written in Japanese.

There is currently very limited supply of microalgae in Australia for use in commercial applications, and development is underway to encourage growth of the microalgae industry with a specific focus on developing a biorefinery system. One of the main aspects of the biorefinery system will be to use waste generated by the production of biofuels in other industries which will provide value addition to the waste products from the production of biodiesel.

Final Report • 2010-11-03 • 1.44 MB
2009-779-DLD-Hons.pdf

Summary

Yellowtail Kingfish is an emerging aquaculture species, and there is an increase in the demand for the fish, both for recreational fishing and for export purposes. This has led to the growth of the aquaculture industry that specialises in culture of yellowtail kingfish in South Australia. Currently very little is known about the physiological properties of yellowtail kingfish, and most data that has been generated on the fish has primarily been in Japan, where most of the scientific articles are written in Japanese.

There is currently very limited supply of microalgae in Australia for use in commercial applications, and development is underway to encourage growth of the microalgae industry with a specific focus on developing a biorefinery system. One of the main aspects of the biorefinery system will be to use waste generated by the production of biofuels in other industries which will provide value addition to the waste products from the production of biodiesel.

Final Report • 2010-11-03 • 1.44 MB
2009-779-DLD-Hons.pdf

Summary

Yellowtail Kingfish is an emerging aquaculture species, and there is an increase in the demand for the fish, both for recreational fishing and for export purposes. This has led to the growth of the aquaculture industry that specialises in culture of yellowtail kingfish in South Australia. Currently very little is known about the physiological properties of yellowtail kingfish, and most data that has been generated on the fish has primarily been in Japan, where most of the scientific articles are written in Japanese.

There is currently very limited supply of microalgae in Australia for use in commercial applications, and development is underway to encourage growth of the microalgae industry with a specific focus on developing a biorefinery system. One of the main aspects of the biorefinery system will be to use waste generated by the production of biofuels in other industries which will provide value addition to the waste products from the production of biodiesel.

Final Report • 2010-11-03 • 1.44 MB
2009-779-DLD-Hons.pdf

Summary

Yellowtail Kingfish is an emerging aquaculture species, and there is an increase in the demand for the fish, both for recreational fishing and for export purposes. This has led to the growth of the aquaculture industry that specialises in culture of yellowtail kingfish in South Australia. Currently very little is known about the physiological properties of yellowtail kingfish, and most data that has been generated on the fish has primarily been in Japan, where most of the scientific articles are written in Japanese.

There is currently very limited supply of microalgae in Australia for use in commercial applications, and development is underway to encourage growth of the microalgae industry with a specific focus on developing a biorefinery system. One of the main aspects of the biorefinery system will be to use waste generated by the production of biofuels in other industries which will provide value addition to the waste products from the production of biodiesel.

SCRC: SCRC RTG 3.12: Micromon recombinant DNA techniques course (Dr James Harris: Students Paula Lima and Celeste Knowles)

Project number: 2009-768
Project Status:
Completed
Budget expenditure: $0.00
Principal Investigator: James O. Harris
Organisation: Flinders University
Project start/end date: 9 Oct 2009 - 29 Dec 2009
Contact:
FRDC

Final report

ISBN: 978-1-925982-18-3
Author: Celeste Knowles
Final Report • 2009-12-30 • 131.15 KB
2009-768-DLD-RTG.pdf

Summary

This research travel grant allowed two PhD students: Paula Lima and Celeste Knowles to travel to Melbourne to undertake the Micromon Recombinant DNA Course conducted by Monash University. This course taught essential techniques and skills which may be used by both students in the course of the PhD studies and throughout their careers.

The Monash Recombinant DNA Techniques Course is widely recognised as the leading course of its type in Australia, which consists of a series of tutorials, lectures and hands on laboratory work.

These students aimed to use the techniques learned to investigate amoebic gill disease in Atlantic Salmon which costs the industry $20-25 million annually. Both students have reported that the techniques learned during the course have been used on a regular basis since returning from Melbourne.

Final Report • 2009-12-30 • 131.15 KB
2009-768-DLD-RTG.pdf

Summary

This research travel grant allowed two PhD students: Paula Lima and Celeste Knowles to travel to Melbourne to undertake the Micromon Recombinant DNA Course conducted by Monash University. This course taught essential techniques and skills which may be used by both students in the course of the PhD studies and throughout their careers.

The Monash Recombinant DNA Techniques Course is widely recognised as the leading course of its type in Australia, which consists of a series of tutorials, lectures and hands on laboratory work.

These students aimed to use the techniques learned to investigate amoebic gill disease in Atlantic Salmon which costs the industry $20-25 million annually. Both students have reported that the techniques learned during the course have been used on a regular basis since returning from Melbourne.

Final Report • 2009-12-30 • 131.15 KB
2009-768-DLD-RTG.pdf

Summary

This research travel grant allowed two PhD students: Paula Lima and Celeste Knowles to travel to Melbourne to undertake the Micromon Recombinant DNA Course conducted by Monash University. This course taught essential techniques and skills which may be used by both students in the course of the PhD studies and throughout their careers.

The Monash Recombinant DNA Techniques Course is widely recognised as the leading course of its type in Australia, which consists of a series of tutorials, lectures and hands on laboratory work.

These students aimed to use the techniques learned to investigate amoebic gill disease in Atlantic Salmon which costs the industry $20-25 million annually. Both students have reported that the techniques learned during the course have been used on a regular basis since returning from Melbourne.

Final Report • 2009-12-30 • 131.15 KB
2009-768-DLD-RTG.pdf

Summary

This research travel grant allowed two PhD students: Paula Lima and Celeste Knowles to travel to Melbourne to undertake the Micromon Recombinant DNA Course conducted by Monash University. This course taught essential techniques and skills which may be used by both students in the course of the PhD studies and throughout their careers.

The Monash Recombinant DNA Techniques Course is widely recognised as the leading course of its type in Australia, which consists of a series of tutorials, lectures and hands on laboratory work.

These students aimed to use the techniques learned to investigate amoebic gill disease in Atlantic Salmon which costs the industry $20-25 million annually. Both students have reported that the techniques learned during the course have been used on a regular basis since returning from Melbourne.

Final Report • 2009-12-30 • 131.15 KB
2009-768-DLD-RTG.pdf

Summary

This research travel grant allowed two PhD students: Paula Lima and Celeste Knowles to travel to Melbourne to undertake the Micromon Recombinant DNA Course conducted by Monash University. This course taught essential techniques and skills which may be used by both students in the course of the PhD studies and throughout their careers.

The Monash Recombinant DNA Techniques Course is widely recognised as the leading course of its type in Australia, which consists of a series of tutorials, lectures and hands on laboratory work.

These students aimed to use the techniques learned to investigate amoebic gill disease in Atlantic Salmon which costs the industry $20-25 million annually. Both students have reported that the techniques learned during the course have been used on a regular basis since returning from Melbourne.

Final Report • 2009-12-30 • 131.15 KB
2009-768-DLD-RTG.pdf

Summary

This research travel grant allowed two PhD students: Paula Lima and Celeste Knowles to travel to Melbourne to undertake the Micromon Recombinant DNA Course conducted by Monash University. This course taught essential techniques and skills which may be used by both students in the course of the PhD studies and throughout their careers.

The Monash Recombinant DNA Techniques Course is widely recognised as the leading course of its type in Australia, which consists of a series of tutorials, lectures and hands on laboratory work.

These students aimed to use the techniques learned to investigate amoebic gill disease in Atlantic Salmon which costs the industry $20-25 million annually. Both students have reported that the techniques learned during the course have been used on a regular basis since returning from Melbourne.

Final Report • 2009-12-30 • 131.15 KB
2009-768-DLD-RTG.pdf

Summary

This research travel grant allowed two PhD students: Paula Lima and Celeste Knowles to travel to Melbourne to undertake the Micromon Recombinant DNA Course conducted by Monash University. This course taught essential techniques and skills which may be used by both students in the course of the PhD studies and throughout their careers.

The Monash Recombinant DNA Techniques Course is widely recognised as the leading course of its type in Australia, which consists of a series of tutorials, lectures and hands on laboratory work.

These students aimed to use the techniques learned to investigate amoebic gill disease in Atlantic Salmon which costs the industry $20-25 million annually. Both students have reported that the techniques learned during the course have been used on a regular basis since returning from Melbourne.

Final Report • 2009-12-30 • 131.15 KB
2009-768-DLD-RTG.pdf

Summary

This research travel grant allowed two PhD students: Paula Lima and Celeste Knowles to travel to Melbourne to undertake the Micromon Recombinant DNA Course conducted by Monash University. This course taught essential techniques and skills which may be used by both students in the course of the PhD studies and throughout their careers.

The Monash Recombinant DNA Techniques Course is widely recognised as the leading course of its type in Australia, which consists of a series of tutorials, lectures and hands on laboratory work.

These students aimed to use the techniques learned to investigate amoebic gill disease in Atlantic Salmon which costs the industry $20-25 million annually. Both students have reported that the techniques learned during the course have been used on a regular basis since returning from Melbourne.

Final Report • 2009-12-30 • 131.15 KB
2009-768-DLD-RTG.pdf

Summary

This research travel grant allowed two PhD students: Paula Lima and Celeste Knowles to travel to Melbourne to undertake the Micromon Recombinant DNA Course conducted by Monash University. This course taught essential techniques and skills which may be used by both students in the course of the PhD studies and throughout their careers.

The Monash Recombinant DNA Techniques Course is widely recognised as the leading course of its type in Australia, which consists of a series of tutorials, lectures and hands on laboratory work.

These students aimed to use the techniques learned to investigate amoebic gill disease in Atlantic Salmon which costs the industry $20-25 million annually. Both students have reported that the techniques learned during the course have been used on a regular basis since returning from Melbourne.

Final Report • 2009-12-30 • 131.15 KB
2009-768-DLD-RTG.pdf

Summary

This research travel grant allowed two PhD students: Paula Lima and Celeste Knowles to travel to Melbourne to undertake the Micromon Recombinant DNA Course conducted by Monash University. This course taught essential techniques and skills which may be used by both students in the course of the PhD studies and throughout their careers.

The Monash Recombinant DNA Techniques Course is widely recognised as the leading course of its type in Australia, which consists of a series of tutorials, lectures and hands on laboratory work.

These students aimed to use the techniques learned to investigate amoebic gill disease in Atlantic Salmon which costs the industry $20-25 million annually. Both students have reported that the techniques learned during the course have been used on a regular basis since returning from Melbourne.

Final Report • 2009-12-30 • 131.15 KB
2009-768-DLD-RTG.pdf

Summary

This research travel grant allowed two PhD students: Paula Lima and Celeste Knowles to travel to Melbourne to undertake the Micromon Recombinant DNA Course conducted by Monash University. This course taught essential techniques and skills which may be used by both students in the course of the PhD studies and throughout their careers.

The Monash Recombinant DNA Techniques Course is widely recognised as the leading course of its type in Australia, which consists of a series of tutorials, lectures and hands on laboratory work.

These students aimed to use the techniques learned to investigate amoebic gill disease in Atlantic Salmon which costs the industry $20-25 million annually. Both students have reported that the techniques learned during the course have been used on a regular basis since returning from Melbourne.

Final Report • 2009-12-30 • 131.15 KB
2009-768-DLD-RTG.pdf

Summary

This research travel grant allowed two PhD students: Paula Lima and Celeste Knowles to travel to Melbourne to undertake the Micromon Recombinant DNA Course conducted by Monash University. This course taught essential techniques and skills which may be used by both students in the course of the PhD studies and throughout their careers.

The Monash Recombinant DNA Techniques Course is widely recognised as the leading course of its type in Australia, which consists of a series of tutorials, lectures and hands on laboratory work.

These students aimed to use the techniques learned to investigate amoebic gill disease in Atlantic Salmon which costs the industry $20-25 million annually. Both students have reported that the techniques learned during the course have been used on a regular basis since returning from Melbourne.

Final Report • 2009-12-30 • 131.15 KB
2009-768-DLD-RTG.pdf

Summary

This research travel grant allowed two PhD students: Paula Lima and Celeste Knowles to travel to Melbourne to undertake the Micromon Recombinant DNA Course conducted by Monash University. This course taught essential techniques and skills which may be used by both students in the course of the PhD studies and throughout their careers.

The Monash Recombinant DNA Techniques Course is widely recognised as the leading course of its type in Australia, which consists of a series of tutorials, lectures and hands on laboratory work.

These students aimed to use the techniques learned to investigate amoebic gill disease in Atlantic Salmon which costs the industry $20-25 million annually. Both students have reported that the techniques learned during the course have been used on a regular basis since returning from Melbourne.

Final Report • 2009-12-30 • 131.15 KB
2009-768-DLD-RTG.pdf

Summary

This research travel grant allowed two PhD students: Paula Lima and Celeste Knowles to travel to Melbourne to undertake the Micromon Recombinant DNA Course conducted by Monash University. This course taught essential techniques and skills which may be used by both students in the course of the PhD studies and throughout their careers.

The Monash Recombinant DNA Techniques Course is widely recognised as the leading course of its type in Australia, which consists of a series of tutorials, lectures and hands on laboratory work.

These students aimed to use the techniques learned to investigate amoebic gill disease in Atlantic Salmon which costs the industry $20-25 million annually. Both students have reported that the techniques learned during the course have been used on a regular basis since returning from Melbourne.

Final Report • 2009-12-30 • 131.15 KB
2009-768-DLD-RTG.pdf

Summary

This research travel grant allowed two PhD students: Paula Lima and Celeste Knowles to travel to Melbourne to undertake the Micromon Recombinant DNA Course conducted by Monash University. This course taught essential techniques and skills which may be used by both students in the course of the PhD studies and throughout their careers.

The Monash Recombinant DNA Techniques Course is widely recognised as the leading course of its type in Australia, which consists of a series of tutorials, lectures and hands on laboratory work.

These students aimed to use the techniques learned to investigate amoebic gill disease in Atlantic Salmon which costs the industry $20-25 million annually. Both students have reported that the techniques learned during the course have been used on a regular basis since returning from Melbourne.

Final Report • 2009-12-30 • 131.15 KB
2009-768-DLD-RTG.pdf

Summary

This research travel grant allowed two PhD students: Paula Lima and Celeste Knowles to travel to Melbourne to undertake the Micromon Recombinant DNA Course conducted by Monash University. This course taught essential techniques and skills which may be used by both students in the course of the PhD studies and throughout their careers.

The Monash Recombinant DNA Techniques Course is widely recognised as the leading course of its type in Australia, which consists of a series of tutorials, lectures and hands on laboratory work.

These students aimed to use the techniques learned to investigate amoebic gill disease in Atlantic Salmon which costs the industry $20-25 million annually. Both students have reported that the techniques learned during the course have been used on a regular basis since returning from Melbourne.

Final Report • 2009-12-30 • 131.15 KB
2009-768-DLD-RTG.pdf

Summary

This research travel grant allowed two PhD students: Paula Lima and Celeste Knowles to travel to Melbourne to undertake the Micromon Recombinant DNA Course conducted by Monash University. This course taught essential techniques and skills which may be used by both students in the course of the PhD studies and throughout their careers.

The Monash Recombinant DNA Techniques Course is widely recognised as the leading course of its type in Australia, which consists of a series of tutorials, lectures and hands on laboratory work.

These students aimed to use the techniques learned to investigate amoebic gill disease in Atlantic Salmon which costs the industry $20-25 million annually. Both students have reported that the techniques learned during the course have been used on a regular basis since returning from Melbourne.

SCRC: SCRC Honours Scholarship H3.2 -Impact of acid sulphate soils on the survival of adult Pacific Oysters challenged by bacterial extracellular products (Dr James Harris: Student Joel Kellam-Stock)

Project number: 2009-764
Project Status:
Completed
Budget expenditure: $0.00
Principal Investigator: James O. Harris
Organisation: Flinders University
Project start/end date: 30 Sep 2009 - 30 Oct 2009
Contact:
FRDC

Final report

ISBN: 978-1-925982-45-9
Author: Joel Kellan-Stock
Final Report • 2009-10-31 • 3.07 MB
2009-764-DLD-Hons.pdf

Summary

This study investigated the impact of short-term exposure to acid sulphate soil leachate on the biochemical condition of the Pacific Oyster Crassostrea gigas. Oysters were exposed to artificially acidified seawater, aluminium and iron treatments for a period of six hours and assessed for changes in mantle glycogen, mantle protein and haemolymph protein between treatments.

An additional challenge using Vibrio harveyi extracellular products was conducted to reduce oyster condition and make them more vulnerable to impacts of acidified treatments. Despite depressed mantle protein levels, no significant differences in biochemical condition were found between control treatments and acidified water treatments indicating that short periods of exposure to acid leachate in the field would be unlikely to have significant impacts on general oyster condition.

Final Report • 2009-10-31 • 3.07 MB
2009-764-DLD-Hons.pdf

Summary

This study investigated the impact of short-term exposure to acid sulphate soil leachate on the biochemical condition of the Pacific Oyster Crassostrea gigas. Oysters were exposed to artificially acidified seawater, aluminium and iron treatments for a period of six hours and assessed for changes in mantle glycogen, mantle protein and haemolymph protein between treatments.

An additional challenge using Vibrio harveyi extracellular products was conducted to reduce oyster condition and make them more vulnerable to impacts of acidified treatments. Despite depressed mantle protein levels, no significant differences in biochemical condition were found between control treatments and acidified water treatments indicating that short periods of exposure to acid leachate in the field would be unlikely to have significant impacts on general oyster condition.

Final Report • 2009-10-31 • 3.07 MB
2009-764-DLD-Hons.pdf

Summary

This study investigated the impact of short-term exposure to acid sulphate soil leachate on the biochemical condition of the Pacific Oyster Crassostrea gigas. Oysters were exposed to artificially acidified seawater, aluminium and iron treatments for a period of six hours and assessed for changes in mantle glycogen, mantle protein and haemolymph protein between treatments.

An additional challenge using Vibrio harveyi extracellular products was conducted to reduce oyster condition and make them more vulnerable to impacts of acidified treatments. Despite depressed mantle protein levels, no significant differences in biochemical condition were found between control treatments and acidified water treatments indicating that short periods of exposure to acid leachate in the field would be unlikely to have significant impacts on general oyster condition.

Final Report • 2009-10-31 • 3.07 MB
2009-764-DLD-Hons.pdf

Summary

This study investigated the impact of short-term exposure to acid sulphate soil leachate on the biochemical condition of the Pacific Oyster Crassostrea gigas. Oysters were exposed to artificially acidified seawater, aluminium and iron treatments for a period of six hours and assessed for changes in mantle glycogen, mantle protein and haemolymph protein between treatments.

An additional challenge using Vibrio harveyi extracellular products was conducted to reduce oyster condition and make them more vulnerable to impacts of acidified treatments. Despite depressed mantle protein levels, no significant differences in biochemical condition were found between control treatments and acidified water treatments indicating that short periods of exposure to acid leachate in the field would be unlikely to have significant impacts on general oyster condition.

Final Report • 2009-10-31 • 3.07 MB
2009-764-DLD-Hons.pdf

Summary

This study investigated the impact of short-term exposure to acid sulphate soil leachate on the biochemical condition of the Pacific Oyster Crassostrea gigas. Oysters were exposed to artificially acidified seawater, aluminium and iron treatments for a period of six hours and assessed for changes in mantle glycogen, mantle protein and haemolymph protein between treatments.

An additional challenge using Vibrio harveyi extracellular products was conducted to reduce oyster condition and make them more vulnerable to impacts of acidified treatments. Despite depressed mantle protein levels, no significant differences in biochemical condition were found between control treatments and acidified water treatments indicating that short periods of exposure to acid leachate in the field would be unlikely to have significant impacts on general oyster condition.

Final Report • 2009-10-31 • 3.07 MB
2009-764-DLD-Hons.pdf

Summary

This study investigated the impact of short-term exposure to acid sulphate soil leachate on the biochemical condition of the Pacific Oyster Crassostrea gigas. Oysters were exposed to artificially acidified seawater, aluminium and iron treatments for a period of six hours and assessed for changes in mantle glycogen, mantle protein and haemolymph protein between treatments.

An additional challenge using Vibrio harveyi extracellular products was conducted to reduce oyster condition and make them more vulnerable to impacts of acidified treatments. Despite depressed mantle protein levels, no significant differences in biochemical condition were found between control treatments and acidified water treatments indicating that short periods of exposure to acid leachate in the field would be unlikely to have significant impacts on general oyster condition.

Final Report • 2009-10-31 • 3.07 MB
2009-764-DLD-Hons.pdf

Summary

This study investigated the impact of short-term exposure to acid sulphate soil leachate on the biochemical condition of the Pacific Oyster Crassostrea gigas. Oysters were exposed to artificially acidified seawater, aluminium and iron treatments for a period of six hours and assessed for changes in mantle glycogen, mantle protein and haemolymph protein between treatments.

An additional challenge using Vibrio harveyi extracellular products was conducted to reduce oyster condition and make them more vulnerable to impacts of acidified treatments. Despite depressed mantle protein levels, no significant differences in biochemical condition were found between control treatments and acidified water treatments indicating that short periods of exposure to acid leachate in the field would be unlikely to have significant impacts on general oyster condition.

Final Report • 2009-10-31 • 3.07 MB
2009-764-DLD-Hons.pdf

Summary

This study investigated the impact of short-term exposure to acid sulphate soil leachate on the biochemical condition of the Pacific Oyster Crassostrea gigas. Oysters were exposed to artificially acidified seawater, aluminium and iron treatments for a period of six hours and assessed for changes in mantle glycogen, mantle protein and haemolymph protein between treatments.

An additional challenge using Vibrio harveyi extracellular products was conducted to reduce oyster condition and make them more vulnerable to impacts of acidified treatments. Despite depressed mantle protein levels, no significant differences in biochemical condition were found between control treatments and acidified water treatments indicating that short periods of exposure to acid leachate in the field would be unlikely to have significant impacts on general oyster condition.

Final Report • 2009-10-31 • 3.07 MB
2009-764-DLD-Hons.pdf

Summary

This study investigated the impact of short-term exposure to acid sulphate soil leachate on the biochemical condition of the Pacific Oyster Crassostrea gigas. Oysters were exposed to artificially acidified seawater, aluminium and iron treatments for a period of six hours and assessed for changes in mantle glycogen, mantle protein and haemolymph protein between treatments.

An additional challenge using Vibrio harveyi extracellular products was conducted to reduce oyster condition and make them more vulnerable to impacts of acidified treatments. Despite depressed mantle protein levels, no significant differences in biochemical condition were found between control treatments and acidified water treatments indicating that short periods of exposure to acid leachate in the field would be unlikely to have significant impacts on general oyster condition.

Final Report • 2009-10-31 • 3.07 MB
2009-764-DLD-Hons.pdf

Summary

This study investigated the impact of short-term exposure to acid sulphate soil leachate on the biochemical condition of the Pacific Oyster Crassostrea gigas. Oysters were exposed to artificially acidified seawater, aluminium and iron treatments for a period of six hours and assessed for changes in mantle glycogen, mantle protein and haemolymph protein between treatments.

An additional challenge using Vibrio harveyi extracellular products was conducted to reduce oyster condition and make them more vulnerable to impacts of acidified treatments. Despite depressed mantle protein levels, no significant differences in biochemical condition were found between control treatments and acidified water treatments indicating that short periods of exposure to acid leachate in the field would be unlikely to have significant impacts on general oyster condition.

Final Report • 2009-10-31 • 3.07 MB
2009-764-DLD-Hons.pdf

Summary

This study investigated the impact of short-term exposure to acid sulphate soil leachate on the biochemical condition of the Pacific Oyster Crassostrea gigas. Oysters were exposed to artificially acidified seawater, aluminium and iron treatments for a period of six hours and assessed for changes in mantle glycogen, mantle protein and haemolymph protein between treatments.

An additional challenge using Vibrio harveyi extracellular products was conducted to reduce oyster condition and make them more vulnerable to impacts of acidified treatments. Despite depressed mantle protein levels, no significant differences in biochemical condition were found between control treatments and acidified water treatments indicating that short periods of exposure to acid leachate in the field would be unlikely to have significant impacts on general oyster condition.

Final Report • 2009-10-31 • 3.07 MB
2009-764-DLD-Hons.pdf

Summary

This study investigated the impact of short-term exposure to acid sulphate soil leachate on the biochemical condition of the Pacific Oyster Crassostrea gigas. Oysters were exposed to artificially acidified seawater, aluminium and iron treatments for a period of six hours and assessed for changes in mantle glycogen, mantle protein and haemolymph protein between treatments.

An additional challenge using Vibrio harveyi extracellular products was conducted to reduce oyster condition and make them more vulnerable to impacts of acidified treatments. Despite depressed mantle protein levels, no significant differences in biochemical condition were found between control treatments and acidified water treatments indicating that short periods of exposure to acid leachate in the field would be unlikely to have significant impacts on general oyster condition.

Final Report • 2009-10-31 • 3.07 MB
2009-764-DLD-Hons.pdf

Summary

This study investigated the impact of short-term exposure to acid sulphate soil leachate on the biochemical condition of the Pacific Oyster Crassostrea gigas. Oysters were exposed to artificially acidified seawater, aluminium and iron treatments for a period of six hours and assessed for changes in mantle glycogen, mantle protein and haemolymph protein between treatments.

An additional challenge using Vibrio harveyi extracellular products was conducted to reduce oyster condition and make them more vulnerable to impacts of acidified treatments. Despite depressed mantle protein levels, no significant differences in biochemical condition were found between control treatments and acidified water treatments indicating that short periods of exposure to acid leachate in the field would be unlikely to have significant impacts on general oyster condition.

Final Report • 2009-10-31 • 3.07 MB
2009-764-DLD-Hons.pdf

Summary

This study investigated the impact of short-term exposure to acid sulphate soil leachate on the biochemical condition of the Pacific Oyster Crassostrea gigas. Oysters were exposed to artificially acidified seawater, aluminium and iron treatments for a period of six hours and assessed for changes in mantle glycogen, mantle protein and haemolymph protein between treatments.

An additional challenge using Vibrio harveyi extracellular products was conducted to reduce oyster condition and make them more vulnerable to impacts of acidified treatments. Despite depressed mantle protein levels, no significant differences in biochemical condition were found between control treatments and acidified water treatments indicating that short periods of exposure to acid leachate in the field would be unlikely to have significant impacts on general oyster condition.

Final Report • 2009-10-31 • 3.07 MB
2009-764-DLD-Hons.pdf

Summary

This study investigated the impact of short-term exposure to acid sulphate soil leachate on the biochemical condition of the Pacific Oyster Crassostrea gigas. Oysters were exposed to artificially acidified seawater, aluminium and iron treatments for a period of six hours and assessed for changes in mantle glycogen, mantle protein and haemolymph protein between treatments.

An additional challenge using Vibrio harveyi extracellular products was conducted to reduce oyster condition and make them more vulnerable to impacts of acidified treatments. Despite depressed mantle protein levels, no significant differences in biochemical condition were found between control treatments and acidified water treatments indicating that short periods of exposure to acid leachate in the field would be unlikely to have significant impacts on general oyster condition.

Final Report • 2009-10-31 • 3.07 MB
2009-764-DLD-Hons.pdf

Summary

This study investigated the impact of short-term exposure to acid sulphate soil leachate on the biochemical condition of the Pacific Oyster Crassostrea gigas. Oysters were exposed to artificially acidified seawater, aluminium and iron treatments for a period of six hours and assessed for changes in mantle glycogen, mantle protein and haemolymph protein between treatments.

An additional challenge using Vibrio harveyi extracellular products was conducted to reduce oyster condition and make them more vulnerable to impacts of acidified treatments. Despite depressed mantle protein levels, no significant differences in biochemical condition were found between control treatments and acidified water treatments indicating that short periods of exposure to acid leachate in the field would be unlikely to have significant impacts on general oyster condition.

Final Report • 2009-10-31 • 3.07 MB
2009-764-DLD-Hons.pdf

Summary

This study investigated the impact of short-term exposure to acid sulphate soil leachate on the biochemical condition of the Pacific Oyster Crassostrea gigas. Oysters were exposed to artificially acidified seawater, aluminium and iron treatments for a period of six hours and assessed for changes in mantle glycogen, mantle protein and haemolymph protein between treatments.

An additional challenge using Vibrio harveyi extracellular products was conducted to reduce oyster condition and make them more vulnerable to impacts of acidified treatments. Despite depressed mantle protein levels, no significant differences in biochemical condition were found between control treatments and acidified water treatments indicating that short periods of exposure to acid leachate in the field would be unlikely to have significant impacts on general oyster condition.

SCRC: SCRC Honours Scholarship H3.1– Impact of commercial hatchery practices on the contribution of broodstock to offspring and genetic diversity in the Yellowtail Kingfish breeding program (Dr Alex Safari: Student Daniel Tan)

Project number: 2009-763
Project Status:
Completed
Budget expenditure: $0.00
Principal Investigator: Alex Safari
Organisation: Flinders University
Project start/end date: 30 Sep 2009 - 29 Sep 2010
Contact:
FRDC

Final report

ISBN: 978-1-925982-29-9
Author: Daniel Tan
Final Report • 2010-09-30 • 342.44 KB
2009-763-DLD-Hons.pdf

Summary

Using small numbers of broodstock for breeding often leads to random drift in gene frequencies between generations. Effective population size (Ne) is used as an indicator to assess the rate of inbreeding and loss of genetic variation in small populations. In this study we used six microsatellites, coupled with DNA parentage analyses, to determine the relative contributions from two commercial Yellowtail Kingfish hatchery broodstock groups over four pooled spawning events (two for each group).

Initial broodstock contributions from the dams was found to be highly skewed, with one dominant dam contributing more than 70% of the offspring (1-day post hatch, dph) in three of the pooled spawning events, while two dams contributed fairly equally to the offspring in the fourth pooled spawning event. Two sires were found to be contributing more that 78% of the offspring in one pooled spawning event, while contributions between the sires were fairly equal in the other three spawning events. The student then examined the differential survival of family size through the hatchery production (from 1 to 25 dph) during which time mortality averaged 80%. Parentage analyses indicated that through the process some maternal and paternal contributions that had been initially represented in the 1-dph offspring 3 were lost. There were also significant changes in the frequencies of progeny from individual dams and sires from day 1 to day 25.

These results indicate that hatchery breeding practices and high mortality during early larvae culture would result in poor retention of genetic variability across generations. With the actual Ne being less than half of the census Ne inbreeding rates would be relatively high if the current broodstock population was closed and future broodstock were derived from existing hatchery systems.

Final Report • 2010-09-30 • 342.44 KB
2009-763-DLD-Hons.pdf

Summary

Using small numbers of broodstock for breeding often leads to random drift in gene frequencies between generations. Effective population size (Ne) is used as an indicator to assess the rate of inbreeding and loss of genetic variation in small populations. In this study we used six microsatellites, coupled with DNA parentage analyses, to determine the relative contributions from two commercial Yellowtail Kingfish hatchery broodstock groups over four pooled spawning events (two for each group).

Initial broodstock contributions from the dams was found to be highly skewed, with one dominant dam contributing more than 70% of the offspring (1-day post hatch, dph) in three of the pooled spawning events, while two dams contributed fairly equally to the offspring in the fourth pooled spawning event. Two sires were found to be contributing more that 78% of the offspring in one pooled spawning event, while contributions between the sires were fairly equal in the other three spawning events. The student then examined the differential survival of family size through the hatchery production (from 1 to 25 dph) during which time mortality averaged 80%. Parentage analyses indicated that through the process some maternal and paternal contributions that had been initially represented in the 1-dph offspring 3 were lost. There were also significant changes in the frequencies of progeny from individual dams and sires from day 1 to day 25.

These results indicate that hatchery breeding practices and high mortality during early larvae culture would result in poor retention of genetic variability across generations. With the actual Ne being less than half of the census Ne inbreeding rates would be relatively high if the current broodstock population was closed and future broodstock were derived from existing hatchery systems.

Final Report • 2010-09-30 • 342.44 KB
2009-763-DLD-Hons.pdf

Summary

Using small numbers of broodstock for breeding often leads to random drift in gene frequencies between generations. Effective population size (Ne) is used as an indicator to assess the rate of inbreeding and loss of genetic variation in small populations. In this study we used six microsatellites, coupled with DNA parentage analyses, to determine the relative contributions from two commercial Yellowtail Kingfish hatchery broodstock groups over four pooled spawning events (two for each group).

Initial broodstock contributions from the dams was found to be highly skewed, with one dominant dam contributing more than 70% of the offspring (1-day post hatch, dph) in three of the pooled spawning events, while two dams contributed fairly equally to the offspring in the fourth pooled spawning event. Two sires were found to be contributing more that 78% of the offspring in one pooled spawning event, while contributions between the sires were fairly equal in the other three spawning events. The student then examined the differential survival of family size through the hatchery production (from 1 to 25 dph) during which time mortality averaged 80%. Parentage analyses indicated that through the process some maternal and paternal contributions that had been initially represented in the 1-dph offspring 3 were lost. There were also significant changes in the frequencies of progeny from individual dams and sires from day 1 to day 25.

These results indicate that hatchery breeding practices and high mortality during early larvae culture would result in poor retention of genetic variability across generations. With the actual Ne being less than half of the census Ne inbreeding rates would be relatively high if the current broodstock population was closed and future broodstock were derived from existing hatchery systems.

Final Report • 2010-09-30 • 342.44 KB
2009-763-DLD-Hons.pdf

Summary

Using small numbers of broodstock for breeding often leads to random drift in gene frequencies between generations. Effective population size (Ne) is used as an indicator to assess the rate of inbreeding and loss of genetic variation in small populations. In this study we used six microsatellites, coupled with DNA parentage analyses, to determine the relative contributions from two commercial Yellowtail Kingfish hatchery broodstock groups over four pooled spawning events (two for each group).

Initial broodstock contributions from the dams was found to be highly skewed, with one dominant dam contributing more than 70% of the offspring (1-day post hatch, dph) in three of the pooled spawning events, while two dams contributed fairly equally to the offspring in the fourth pooled spawning event. Two sires were found to be contributing more that 78% of the offspring in one pooled spawning event, while contributions between the sires were fairly equal in the other three spawning events. The student then examined the differential survival of family size through the hatchery production (from 1 to 25 dph) during which time mortality averaged 80%. Parentage analyses indicated that through the process some maternal and paternal contributions that had been initially represented in the 1-dph offspring 3 were lost. There were also significant changes in the frequencies of progeny from individual dams and sires from day 1 to day 25.

These results indicate that hatchery breeding practices and high mortality during early larvae culture would result in poor retention of genetic variability across generations. With the actual Ne being less than half of the census Ne inbreeding rates would be relatively high if the current broodstock population was closed and future broodstock were derived from existing hatchery systems.

Final Report • 2010-09-30 • 342.44 KB
2009-763-DLD-Hons.pdf

Summary

Using small numbers of broodstock for breeding often leads to random drift in gene frequencies between generations. Effective population size (Ne) is used as an indicator to assess the rate of inbreeding and loss of genetic variation in small populations. In this study we used six microsatellites, coupled with DNA parentage analyses, to determine the relative contributions from two commercial Yellowtail Kingfish hatchery broodstock groups over four pooled spawning events (two for each group).

Initial broodstock contributions from the dams was found to be highly skewed, with one dominant dam contributing more than 70% of the offspring (1-day post hatch, dph) in three of the pooled spawning events, while two dams contributed fairly equally to the offspring in the fourth pooled spawning event. Two sires were found to be contributing more that 78% of the offspring in one pooled spawning event, while contributions between the sires were fairly equal in the other three spawning events. The student then examined the differential survival of family size through the hatchery production (from 1 to 25 dph) during which time mortality averaged 80%. Parentage analyses indicated that through the process some maternal and paternal contributions that had been initially represented in the 1-dph offspring 3 were lost. There were also significant changes in the frequencies of progeny from individual dams and sires from day 1 to day 25.

These results indicate that hatchery breeding practices and high mortality during early larvae culture would result in poor retention of genetic variability across generations. With the actual Ne being less than half of the census Ne inbreeding rates would be relatively high if the current broodstock population was closed and future broodstock were derived from existing hatchery systems.

Final Report • 2010-09-30 • 342.44 KB
2009-763-DLD-Hons.pdf

Summary

Using small numbers of broodstock for breeding often leads to random drift in gene frequencies between generations. Effective population size (Ne) is used as an indicator to assess the rate of inbreeding and loss of genetic variation in small populations. In this study we used six microsatellites, coupled with DNA parentage analyses, to determine the relative contributions from two commercial Yellowtail Kingfish hatchery broodstock groups over four pooled spawning events (two for each group).

Initial broodstock contributions from the dams was found to be highly skewed, with one dominant dam contributing more than 70% of the offspring (1-day post hatch, dph) in three of the pooled spawning events, while two dams contributed fairly equally to the offspring in the fourth pooled spawning event. Two sires were found to be contributing more that 78% of the offspring in one pooled spawning event, while contributions between the sires were fairly equal in the other three spawning events. The student then examined the differential survival of family size through the hatchery production (from 1 to 25 dph) during which time mortality averaged 80%. Parentage analyses indicated that through the process some maternal and paternal contributions that had been initially represented in the 1-dph offspring 3 were lost. There were also significant changes in the frequencies of progeny from individual dams and sires from day 1 to day 25.

These results indicate that hatchery breeding practices and high mortality during early larvae culture would result in poor retention of genetic variability across generations. With the actual Ne being less than half of the census Ne inbreeding rates would be relatively high if the current broodstock population was closed and future broodstock were derived from existing hatchery systems.

Final Report • 2010-09-30 • 342.44 KB
2009-763-DLD-Hons.pdf

Summary

Using small numbers of broodstock for breeding often leads to random drift in gene frequencies between generations. Effective population size (Ne) is used as an indicator to assess the rate of inbreeding and loss of genetic variation in small populations. In this study we used six microsatellites, coupled with DNA parentage analyses, to determine the relative contributions from two commercial Yellowtail Kingfish hatchery broodstock groups over four pooled spawning events (two for each group).

Initial broodstock contributions from the dams was found to be highly skewed, with one dominant dam contributing more than 70% of the offspring (1-day post hatch, dph) in three of the pooled spawning events, while two dams contributed fairly equally to the offspring in the fourth pooled spawning event. Two sires were found to be contributing more that 78% of the offspring in one pooled spawning event, while contributions between the sires were fairly equal in the other three spawning events. The student then examined the differential survival of family size through the hatchery production (from 1 to 25 dph) during which time mortality averaged 80%. Parentage analyses indicated that through the process some maternal and paternal contributions that had been initially represented in the 1-dph offspring 3 were lost. There were also significant changes in the frequencies of progeny from individual dams and sires from day 1 to day 25.

These results indicate that hatchery breeding practices and high mortality during early larvae culture would result in poor retention of genetic variability across generations. With the actual Ne being less than half of the census Ne inbreeding rates would be relatively high if the current broodstock population was closed and future broodstock were derived from existing hatchery systems.

Final Report • 2010-09-30 • 342.44 KB
2009-763-DLD-Hons.pdf

Summary

Using small numbers of broodstock for breeding often leads to random drift in gene frequencies between generations. Effective population size (Ne) is used as an indicator to assess the rate of inbreeding and loss of genetic variation in small populations. In this study we used six microsatellites, coupled with DNA parentage analyses, to determine the relative contributions from two commercial Yellowtail Kingfish hatchery broodstock groups over four pooled spawning events (two for each group).

Initial broodstock contributions from the dams was found to be highly skewed, with one dominant dam contributing more than 70% of the offspring (1-day post hatch, dph) in three of the pooled spawning events, while two dams contributed fairly equally to the offspring in the fourth pooled spawning event. Two sires were found to be contributing more that 78% of the offspring in one pooled spawning event, while contributions between the sires were fairly equal in the other three spawning events. The student then examined the differential survival of family size through the hatchery production (from 1 to 25 dph) during which time mortality averaged 80%. Parentage analyses indicated that through the process some maternal and paternal contributions that had been initially represented in the 1-dph offspring 3 were lost. There were also significant changes in the frequencies of progeny from individual dams and sires from day 1 to day 25.

These results indicate that hatchery breeding practices and high mortality during early larvae culture would result in poor retention of genetic variability across generations. With the actual Ne being less than half of the census Ne inbreeding rates would be relatively high if the current broodstock population was closed and future broodstock were derived from existing hatchery systems.

Final Report • 2010-09-30 • 342.44 KB
2009-763-DLD-Hons.pdf

Summary

Using small numbers of broodstock for breeding often leads to random drift in gene frequencies between generations. Effective population size (Ne) is used as an indicator to assess the rate of inbreeding and loss of genetic variation in small populations. In this study we used six microsatellites, coupled with DNA parentage analyses, to determine the relative contributions from two commercial Yellowtail Kingfish hatchery broodstock groups over four pooled spawning events (two for each group).

Initial broodstock contributions from the dams was found to be highly skewed, with one dominant dam contributing more than 70% of the offspring (1-day post hatch, dph) in three of the pooled spawning events, while two dams contributed fairly equally to the offspring in the fourth pooled spawning event. Two sires were found to be contributing more that 78% of the offspring in one pooled spawning event, while contributions between the sires were fairly equal in the other three spawning events. The student then examined the differential survival of family size through the hatchery production (from 1 to 25 dph) during which time mortality averaged 80%. Parentage analyses indicated that through the process some maternal and paternal contributions that had been initially represented in the 1-dph offspring 3 were lost. There were also significant changes in the frequencies of progeny from individual dams and sires from day 1 to day 25.

These results indicate that hatchery breeding practices and high mortality during early larvae culture would result in poor retention of genetic variability across generations. With the actual Ne being less than half of the census Ne inbreeding rates would be relatively high if the current broodstock population was closed and future broodstock were derived from existing hatchery systems.

Final Report • 2010-09-30 • 342.44 KB
2009-763-DLD-Hons.pdf

Summary

Using small numbers of broodstock for breeding often leads to random drift in gene frequencies between generations. Effective population size (Ne) is used as an indicator to assess the rate of inbreeding and loss of genetic variation in small populations. In this study we used six microsatellites, coupled with DNA parentage analyses, to determine the relative contributions from two commercial Yellowtail Kingfish hatchery broodstock groups over four pooled spawning events (two for each group).

Initial broodstock contributions from the dams was found to be highly skewed, with one dominant dam contributing more than 70% of the offspring (1-day post hatch, dph) in three of the pooled spawning events, while two dams contributed fairly equally to the offspring in the fourth pooled spawning event. Two sires were found to be contributing more that 78% of the offspring in one pooled spawning event, while contributions between the sires were fairly equal in the other three spawning events. The student then examined the differential survival of family size through the hatchery production (from 1 to 25 dph) during which time mortality averaged 80%. Parentage analyses indicated that through the process some maternal and paternal contributions that had been initially represented in the 1-dph offspring 3 were lost. There were also significant changes in the frequencies of progeny from individual dams and sires from day 1 to day 25.

These results indicate that hatchery breeding practices and high mortality during early larvae culture would result in poor retention of genetic variability across generations. With the actual Ne being less than half of the census Ne inbreeding rates would be relatively high if the current broodstock population was closed and future broodstock were derived from existing hatchery systems.

Final Report • 2010-09-30 • 342.44 KB
2009-763-DLD-Hons.pdf

Summary

Using small numbers of broodstock for breeding often leads to random drift in gene frequencies between generations. Effective population size (Ne) is used as an indicator to assess the rate of inbreeding and loss of genetic variation in small populations. In this study we used six microsatellites, coupled with DNA parentage analyses, to determine the relative contributions from two commercial Yellowtail Kingfish hatchery broodstock groups over four pooled spawning events (two for each group).

Initial broodstock contributions from the dams was found to be highly skewed, with one dominant dam contributing more than 70% of the offspring (1-day post hatch, dph) in three of the pooled spawning events, while two dams contributed fairly equally to the offspring in the fourth pooled spawning event. Two sires were found to be contributing more that 78% of the offspring in one pooled spawning event, while contributions between the sires were fairly equal in the other three spawning events. The student then examined the differential survival of family size through the hatchery production (from 1 to 25 dph) during which time mortality averaged 80%. Parentage analyses indicated that through the process some maternal and paternal contributions that had been initially represented in the 1-dph offspring 3 were lost. There were also significant changes in the frequencies of progeny from individual dams and sires from day 1 to day 25.

These results indicate that hatchery breeding practices and high mortality during early larvae culture would result in poor retention of genetic variability across generations. With the actual Ne being less than half of the census Ne inbreeding rates would be relatively high if the current broodstock population was closed and future broodstock were derived from existing hatchery systems.

Final Report • 2010-09-30 • 342.44 KB
2009-763-DLD-Hons.pdf

Summary

Using small numbers of broodstock for breeding often leads to random drift in gene frequencies between generations. Effective population size (Ne) is used as an indicator to assess the rate of inbreeding and loss of genetic variation in small populations. In this study we used six microsatellites, coupled with DNA parentage analyses, to determine the relative contributions from two commercial Yellowtail Kingfish hatchery broodstock groups over four pooled spawning events (two for each group).

Initial broodstock contributions from the dams was found to be highly skewed, with one dominant dam contributing more than 70% of the offspring (1-day post hatch, dph) in three of the pooled spawning events, while two dams contributed fairly equally to the offspring in the fourth pooled spawning event. Two sires were found to be contributing more that 78% of the offspring in one pooled spawning event, while contributions between the sires were fairly equal in the other three spawning events. The student then examined the differential survival of family size through the hatchery production (from 1 to 25 dph) during which time mortality averaged 80%. Parentage analyses indicated that through the process some maternal and paternal contributions that had been initially represented in the 1-dph offspring 3 were lost. There were also significant changes in the frequencies of progeny from individual dams and sires from day 1 to day 25.

These results indicate that hatchery breeding practices and high mortality during early larvae culture would result in poor retention of genetic variability across generations. With the actual Ne being less than half of the census Ne inbreeding rates would be relatively high if the current broodstock population was closed and future broodstock were derived from existing hatchery systems.

Final Report • 2010-09-30 • 342.44 KB
2009-763-DLD-Hons.pdf

Summary

Using small numbers of broodstock for breeding often leads to random drift in gene frequencies between generations. Effective population size (Ne) is used as an indicator to assess the rate of inbreeding and loss of genetic variation in small populations. In this study we used six microsatellites, coupled with DNA parentage analyses, to determine the relative contributions from two commercial Yellowtail Kingfish hatchery broodstock groups over four pooled spawning events (two for each group).

Initial broodstock contributions from the dams was found to be highly skewed, with one dominant dam contributing more than 70% of the offspring (1-day post hatch, dph) in three of the pooled spawning events, while two dams contributed fairly equally to the offspring in the fourth pooled spawning event. Two sires were found to be contributing more that 78% of the offspring in one pooled spawning event, while contributions between the sires were fairly equal in the other three spawning events. The student then examined the differential survival of family size through the hatchery production (from 1 to 25 dph) during which time mortality averaged 80%. Parentage analyses indicated that through the process some maternal and paternal contributions that had been initially represented in the 1-dph offspring 3 were lost. There were also significant changes in the frequencies of progeny from individual dams and sires from day 1 to day 25.

These results indicate that hatchery breeding practices and high mortality during early larvae culture would result in poor retention of genetic variability across generations. With the actual Ne being less than half of the census Ne inbreeding rates would be relatively high if the current broodstock population was closed and future broodstock were derived from existing hatchery systems.

Final Report • 2010-09-30 • 342.44 KB
2009-763-DLD-Hons.pdf

Summary

Using small numbers of broodstock for breeding often leads to random drift in gene frequencies between generations. Effective population size (Ne) is used as an indicator to assess the rate of inbreeding and loss of genetic variation in small populations. In this study we used six microsatellites, coupled with DNA parentage analyses, to determine the relative contributions from two commercial Yellowtail Kingfish hatchery broodstock groups over four pooled spawning events (two for each group).

Initial broodstock contributions from the dams was found to be highly skewed, with one dominant dam contributing more than 70% of the offspring (1-day post hatch, dph) in three of the pooled spawning events, while two dams contributed fairly equally to the offspring in the fourth pooled spawning event. Two sires were found to be contributing more that 78% of the offspring in one pooled spawning event, while contributions between the sires were fairly equal in the other three spawning events. The student then examined the differential survival of family size through the hatchery production (from 1 to 25 dph) during which time mortality averaged 80%. Parentage analyses indicated that through the process some maternal and paternal contributions that had been initially represented in the 1-dph offspring 3 were lost. There were also significant changes in the frequencies of progeny from individual dams and sires from day 1 to day 25.

These results indicate that hatchery breeding practices and high mortality during early larvae culture would result in poor retention of genetic variability across generations. With the actual Ne being less than half of the census Ne inbreeding rates would be relatively high if the current broodstock population was closed and future broodstock were derived from existing hatchery systems.

Final Report • 2010-09-30 • 342.44 KB
2009-763-DLD-Hons.pdf

Summary

Using small numbers of broodstock for breeding often leads to random drift in gene frequencies between generations. Effective population size (Ne) is used as an indicator to assess the rate of inbreeding and loss of genetic variation in small populations. In this study we used six microsatellites, coupled with DNA parentage analyses, to determine the relative contributions from two commercial Yellowtail Kingfish hatchery broodstock groups over four pooled spawning events (two for each group).

Initial broodstock contributions from the dams was found to be highly skewed, with one dominant dam contributing more than 70% of the offspring (1-day post hatch, dph) in three of the pooled spawning events, while two dams contributed fairly equally to the offspring in the fourth pooled spawning event. Two sires were found to be contributing more that 78% of the offspring in one pooled spawning event, while contributions between the sires were fairly equal in the other three spawning events. The student then examined the differential survival of family size through the hatchery production (from 1 to 25 dph) during which time mortality averaged 80%. Parentage analyses indicated that through the process some maternal and paternal contributions that had been initially represented in the 1-dph offspring 3 were lost. There were also significant changes in the frequencies of progeny from individual dams and sires from day 1 to day 25.

These results indicate that hatchery breeding practices and high mortality during early larvae culture would result in poor retention of genetic variability across generations. With the actual Ne being less than half of the census Ne inbreeding rates would be relatively high if the current broodstock population was closed and future broodstock were derived from existing hatchery systems.

Final Report • 2010-09-30 • 342.44 KB
2009-763-DLD-Hons.pdf

Summary

Using small numbers of broodstock for breeding often leads to random drift in gene frequencies between generations. Effective population size (Ne) is used as an indicator to assess the rate of inbreeding and loss of genetic variation in small populations. In this study we used six microsatellites, coupled with DNA parentage analyses, to determine the relative contributions from two commercial Yellowtail Kingfish hatchery broodstock groups over four pooled spawning events (two for each group).

Initial broodstock contributions from the dams was found to be highly skewed, with one dominant dam contributing more than 70% of the offspring (1-day post hatch, dph) in three of the pooled spawning events, while two dams contributed fairly equally to the offspring in the fourth pooled spawning event. Two sires were found to be contributing more that 78% of the offspring in one pooled spawning event, while contributions between the sires were fairly equal in the other three spawning events. The student then examined the differential survival of family size through the hatchery production (from 1 to 25 dph) during which time mortality averaged 80%. Parentage analyses indicated that through the process some maternal and paternal contributions that had been initially represented in the 1-dph offspring 3 were lost. There were also significant changes in the frequencies of progeny from individual dams and sires from day 1 to day 25.

These results indicate that hatchery breeding practices and high mortality during early larvae culture would result in poor retention of genetic variability across generations. With the actual Ne being less than half of the census Ne inbreeding rates would be relatively high if the current broodstock population was closed and future broodstock were derived from existing hatchery systems.

Final Report • 2010-09-30 • 342.44 KB
2009-763-DLD-Hons.pdf

Summary

Using small numbers of broodstock for breeding often leads to random drift in gene frequencies between generations. Effective population size (Ne) is used as an indicator to assess the rate of inbreeding and loss of genetic variation in small populations. In this study we used six microsatellites, coupled with DNA parentage analyses, to determine the relative contributions from two commercial Yellowtail Kingfish hatchery broodstock groups over four pooled spawning events (two for each group).

Initial broodstock contributions from the dams was found to be highly skewed, with one dominant dam contributing more than 70% of the offspring (1-day post hatch, dph) in three of the pooled spawning events, while two dams contributed fairly equally to the offspring in the fourth pooled spawning event. Two sires were found to be contributing more that 78% of the offspring in one pooled spawning event, while contributions between the sires were fairly equal in the other three spawning events. The student then examined the differential survival of family size through the hatchery production (from 1 to 25 dph) during which time mortality averaged 80%. Parentage analyses indicated that through the process some maternal and paternal contributions that had been initially represented in the 1-dph offspring 3 were lost. There were also significant changes in the frequencies of progeny from individual dams and sires from day 1 to day 25.

These results indicate that hatchery breeding practices and high mortality during early larvae culture would result in poor retention of genetic variability across generations. With the actual Ne being less than half of the census Ne inbreeding rates would be relatively high if the current broodstock population was closed and future broodstock were derived from existing hatchery systems.

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