Condition and its assessment in the southern rock lobster. Field application of the techniques for condition assessment developed in the laboratory
Final report
Survey sampling design and length-frequency data analysis for on-going monitoring and model parameter evaluation in the South Australian rock lobster fishery
Final report
Feasibility assessment of an adaptive management experiment in the SA lobster fishery
Immuno-staining of a ciliate protozoan causing significant mortalilty of farmed tuna: the development of a rapid identification technique which will enable improved farm management practices to be implemented to minimise fish mortality
Final report
An immunofluorescent staining technique for the rapid detection of the ciliate protozoan Uronema sp. was developed during 1995 and 1996. The initial test was developed using seven cultures of Uronema sp. from various sources which were identified as Uronema nigricans by microscopical and histochemical techniques. These seven strains were maintained in a medium containing bacteria as their food source. Antisera against two of the strains were raised in rabbits and against one in sheep. These were then adsorbed with the bacteria and unrelated ciliates to eliminate cross-reactivity.
More recently, an axenic (bacteria-free) culture of Uronema sp. has been accomplished and high-titre antisera raised in rabbits for use in the immunofluorescent staining technique.
Arrangements have been made to have a monoclonal antibody to the axenic Uronema prepared at the Atlantic Veterinary College, University of Prince Edward Island in Canada. This will then provide a reliable supply of highly specific antibody for future research in this area.
The substantial southern bluefin tuna mortality event in 1996 impacted on the delivery of the project objectives, particularly those associated with the application of the immuno-staining technique developed to:
- screen potential sources of infection associated with farming tuna; and based on this
- provide advice to farmers on how to improve farm management techniques so as to minimise infections.
Keywords: Uronema, aquaculture, southern bluefin tuna.
Development of an in vitro assay for the assessment of alternative protein sources for use in artificial diets for farmed southern bluefin tuna
Final report
Condition and its assessment in the southern rock lobster (Jasus edwardsii). Assessment of condition indices and moult staging techniques
Final report
Development of an integrated fisheries management model for King George whiting (Sillaginodes punctata) in South Australia
Guide
The King George Whiting Simulator (WhitSim) is a simulation version of the Whiting Estimator (WhitEst) encased inside a graphical user interface (GUI) that includes geographical information systems (GIS). WhitSim provides the ability to simulate various management strategies and parameter scenarios and interrogate results of the simulation via in-built statistical, curve fitting and indicator analysis modules.
Project products
Stock Identification and discrimination of snapper (Pagrus auratus) in South Australian waters, using molecular genetic techniques
Final report
Studies of genetic variation in the proteins and DNA of snapper were carried out at the South Australian Museum to determine the presence of genetically isolated breeding stocks. Fish from each of the three commercially fished areas within the state and from Victoria and Western Australia were sampled over a 5 year period with assistance from commercial and recreational fishers.
The present genetic study provided no evidence of the existence of more than one stock in the commercially fished waters in S.A. and south-western W.A. This is consistent with tagging studies carried out in South Australia over the past 19 years. In contrast, both genetic and tagging studies have shown that fish from Port Phillip Bay, Victoria represent a different stock which extends into the south-east of S.A.
The significance of these results is that the snapper fishery in S.A. should be managed as a single genetic stock. Ultimately, final management of the fishery will incorporate other scientific and economic parameters, but will have a stronger biological basis because of the availability of these genetic data.