Workshop to identify research needs and a future project to reduce bycatch and improve fuel efficiency via Low Impact Fuel Efficient (LIFE) prawn trawls
Issues of bycatch and fuel efficiency are now becoming uppermost in the concerns of many stakeholders. These include: the industry itself (which wishes to reduce running costs and discard handling), environmental groups (who are concerned about ecosystem disturbance and energy use), eco-labelling agencies (whose requirements often focus on bycatch and habitat impacts), and the general public (who dictate the “social licence to operate” for such fisheries). These issues have therefore attracted the attention of many governments as well as international agencies like the FAO who first coined the term Low Impact Fuel Efficient gears (LIFE) for methods that reduce bycatch whilst improving fuel efficiency.
However, there have been only a few studies that address these issues. And one of the centres where this work has occurred is the NSW Conservation Technology Unit. In recent years, Dr Broadhurst from this group applied for FRDC funds to enhance LIFE research by focussing on the prawn fisheries in Australia. And his most recent application led to the need for this current application to hold a workshop of the relevant prawn fisheries in Australia to develop the foci, objectives and way-forward for this important research.
Final report
IPA APFA: detection of pesticide impacts on larval prawns in hatcheries and presence in estuarine intake water
It is possible that by drawing water supply from pesticide contaminated estuaries, prawn farmers are inadvertently lowering their larval survival rates or are depressing growth efficiency because the pesticide mixtures in these waterways are causing toxic effects. It is also possible that the survival and growth efficiency of post larval stages is depressed by stocking into ponds with either contaminated sediment or contaminated water. While levels of imidacloprid measured by DSITI in the freshwater sections of some Queensland rivers would be sufficient to cause harm in some crustaceans, we do not know what the pesticide concentrations at the farms themselves are, especially not for the entire suite of potential toxicants currently in use, nor do we know how sensitive prawn larvae are to exposure to modern use pesticides. Once this information is available, we can perform a risk assessment for the aquaculture industry.
The aquaculture industry need this information about the risks to their operations posed by pesticide exposure to be able to accurately assess whether or not modern use pesticides pose a risk to their hatchery and pond stocking operations.
Final report
Proposed northern Australia water developments pertinent to the Northern Prawn Fishery: collation and review
Seafood CRC: sustainability Certification Australian farmed prawns
Seafood CRC: Australian Council of Prawn Fisheries (ACPF) Strategic Plan and Business Plan
APFA IPA: RNAi treatment of broodstock to reduce disease impacts in farmed prawns
With the exception for one farm, the Australian prawn farming industry remains reliant on the use of wild broodstock in hatcheries to generate seed to stock farm ponds,. Due partly to problems with GAV often occurring at high prevalence in North Queensland (NQ) (eg. Etty Bay, Bingil Bay, Bramston Beach, Yorkeys Knob), GAV-free broodstock have increasingly been sought from more remote locations in the Northern Territory (NT) (eg. Joseph Bonaparte Gulf and Melville/Tiwi Island) (Cowley et al. 2016).
Broodstock pairs (male + female) typically cost ~$160 when sourced locally from NQ and ~$600 when sourced from NT. Hatcheries use in the order of 3,000 wild-caught broodstock pairs annually. Thus due to (i) the substantially higher costs of broodstock sourced from remote regions in NT, (ii) the detection of a GAV variant designated Yellow head virus genotype 7 (YHV7) amongst NT stock (Cowley et al. 2016) with commercially relevant pathogenic potential (CSIRO Unpublished data) and (iii) difficulties in supply continuity and transport stress, the use of a hatchery-based RNAi strategy to reduce or clear GAV infection from local NQ broodstock with potential to abrogate or curtail the vertical transmission of GAV to progeny would significantly benefit industry.
Proof-of-concept obtained in this project that RNAi can be up-scaled from experimental to hatchery-scale systems, and that progeny with markedly reduced GAV infection loads can be generated from carrier broodstock injected with dsRNA, will provide industry with the confidence needed to apply the technology commercially once an APVMA permit has been issued for its use.
Report
As these project objectives were revised due to difficulties in sourcing wild broodstock infected with suitably high loads of GAV, also reported are data from agreed alternative project objectives showing that (i) Infectious hypodermal and hematopoietic necrosis virus (IHHNV) is transmitted vertically from infected female broodstock to progeny and that the IHHNV prevalence and infection loads in progeny are influenced by infection loads in their parental female and (ii) the high-load infections that develop in progeny spawned from females with higher-level IHHNV infection result in substantially reduced growth performance and survival of progeny reared in 0.16 ha ponds under simulated commercial conditions.
The screening of batches of wild P. monodon broodstock to identify locations where these might be infected with GAV at moderately-high loads suitable for the original project objectives identified GAV to be present at very low prevalence among prawns captured at various locations in the vicinity of Innisfail between May and June 2016. Similar screening also identified the absence of Yellow head virus genotype 7 (YHV7) in these broodstock.
Further to these objectives and data, it was agreed to include another project variation objective to investigate whether (i) dsRNA(s) injected into tail-muscle of female broodstock at the time eyestalks were ablated to induce ovary maturation/spawning could be detected by TaqMan real-time RT-qPCR in various tissues (i.e. pleopod, ovary and lymphoid organ) several days later when the female spawned and (ii) dsRNA might transfer from injected females to spawned eggs and be maintained or amplified through larval life stages (i.e. nauplii, protozoea, mysis) to an early post-larvae (PL) stage
APFA IPA: towards understanding the relationship of the distribution of the PirAB toxin DNA and Penaeus monodon mortality syndrome (PMMS) pathology in farmed prawns in Australia
Determining the susceptibility of Australian Penaeus monodon and Penaeus merguiensis to newly identified enzootic (YHV7) and exotic (YHV8 and YHV10) Yellow head virus (YHV) genotypes
Australian prawn production, forecast at 24 kilotonnes in 2014/15, is valued at >$310 million. The prawn fishery is an important natural resource that supports a substantial export industry. Prawn aquaculture is a significant industry in northern Australia and accounts for approximately 20% of the total volume of Australian production.
Of the YHV-complex of viruses, YHV1 is exotic to Australia, is an OIE-listed pathogen and can cause 100% mortality in 3-5 days. The endemic GAV (YHV2) occurs commonly in P. monodon in Australia and has been associated with Mid-Crop Mortality Syndrome (MCMS). The pathogenicity of the newly detected YHV-complex genotypes to Australian prawns is unknown. While YHV7 was detected in diseased P. monodon its role in the disease is unknown. YHV8 and YHV10 were detected in imported commodity prawns from China. These prawns were released from quarantine as only YHV1 requires risk management according to the prawn IRA (BAA 2009/25). The pathogenicity and potential impact of YHV8 and YHV10 to Australian prawns is unknown and the risk associated with these imported genotypes needs to be clarified.
This project will generate knowledge regarding the susceptibility of Australian farmed P. monodon and P. merguiensis to the newly discovered enzootic YHV7 and exotic YHV8 and YHV10 genotypes. This information is critical for policy-makers, regulators, managers and producers to implement appropriate biosecurity measures. The Project aligns with Key Research Area 6.2.1 of the FRDC AAHS R&D Plan “Knowledge about new and emerging infectious diseases” and value-adds to the FRDC 2103/036 TRF project.
Final report
In recent years, new genotypes within the yellow head complex of viruses have been discovered in farmed prawns both within Australia and overseas. This report describes research undertaken at the CSIRO Australian Centre for Disease Preparedness Fish Diseases Laboratory (ACDP AFDL), Geelong, Victoria to determine the pathogenicity of three of these new genotypes to prawn species significant to the Australian prawn farming industry. As part of the project, new diagnostic tests (RT-qPCR assays) were evaluated and determined to be highly sensitive and specific and can be used for monitoring of domestic prawn populations as well as commodity prawns imported into Australia, enhancing Australia’s biosecurity capability.