Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.