Project number:
2001-624
Project Status:
Completed
Budget expenditure:
$156,017.00
Principal Investigator:
Mark S. Crane
Organisation:
CSIRO Australian Animal Health Laboratory
Project start/end date:
13 Oct 2001
-
25 Jun 2004
Contact:
FRDC
SPECIES
1. To establish and optimize a cell culture system for the isolation and growth of P.salmonis.
2. To develop an immunohistochemical assay using available specific monoclonal antibodies and polyclonal antisera for the identification of P.salmonis in fish tissue.
3. To develop and optimise a PCR assay for the identification and distinction of P.salmonis strains present in cell culture supernatant and/or in fish tissues.
4. To compare the nucleic acid sequence of the emerging Tasmanian isolates of P.salmonis to sequences of known exotic strains.
5. Purify cell culture derived P.salmonis and raise specific polyclonal antisera in rabbits.
6. A molecular probe suitable for in situ hybridisation use on tissue sections will be developed, evaluated and made available to diagnostic laboratories.
