1. The objectives corresponding to the four research priorities of this study are explained in the following list of Subprojects: Objective 1. Subproject 1: Run a Technology Transfer Facilitation Framework Meeting with CST staff, and project R&D participants. Subproject 2: Develop a growth-feed intake model specific to YTK. Subproject 3: Test and validate the growth-feed intake model developed in Subproject 2 with YTK cultured in replicated sea cages on the CST R&D farm at summer water temperatures.
2. Objective 2: Determination of cage biomass. Subproject 4: Review current fish counting methods used by CST to establish a more accurate on-farm fish counting system. Subproject 5: Calibrate, validate and implement two electronic fish biomass counter systems to CST YTK sea cages for the determination of YTK biomass.
3. Objective 3: Clarify the protein to energy ratios of ~1 to 4 kg YTK. Subproject 6: Test the effects of varying protein to energy ratios on the growth performance, and feed efficiency of ~1 to 4 kg YTK cultured in sea cages in the CST research farm
4. Objective 4: Improve the sustainability of YTK farming by investigating the maximum inclusion levels of alternative protein and lipid sources to replace fish meal and fish oil, at optimal (22°C) and suboptimal (18°C) temperatures. Subproject 7: Investigate the suitability of canola oil, and poultry fat as alternative sources to fish oil for YTK when cultured at optimal and sub-optimal temperatures. Subproject 8: Explore the suitability of soybean products as alternative protein sources (solvent extracted soy 48 and soy protein concentrate) in juvenile diets at optimal and sub-optimal temperatures. Subproject 9: Test the effects of alterative protein sources on the growth performance of ~4 kg YTK cultured in the CST research farm. Subproject 10: Evaluate the enzyme activity of YTK under a range of temperatures (8-28oC).