13 results
Industry
Industry
PROJECT NUMBER • 2011-761
PROJECT STATUS:
COMPLETED

Seafood CRC: optimisation of viral clearance from broodstock prawns using targeted RNA interference

Economic losses due to diseases mostly caused by viruses remain a major obstacle to realizing the production potential of prawn aquaculture industries in many parts of the world. Broodstock used in prawn hatcheries in Australia are generally managed quite intensively and numbers used are generally...
ORGANISATION:
CSIRO Oceans and Atmosphere Hobart
Industry
PROJECT NUMBER • 2008-756
PROJECT STATUS:
COMPLETED

Seafood CRC: increasing seedstock production of domesticated giant tiger prawns (Penaeus monodon) through improved male fertility

Given the rationale that pond systems are likely the most cost-effective system for large-scale production of Giant Tiger Prawn (Penaeus monodon) broodstock, this project aimed to determine whether pond-rearing poses a significant risk for broodstock production. The gross reproductive development of...
ORGANISATION:
CSIRO Oceans and Atmosphere Hobart

DNA microsatellite variation in Atlantic salmon

Project number: 1995-080
Project Status:
Completed
Budget expenditure: $93,794.00
Principal Investigator: Nick G. Elliott
Organisation: CSIRO Oceans and Atmosphere Hobart
Project start/end date: 29 Jul 1995 - 31 Jul 1997
Contact:
FRDC

Objectives

1. To develop DNA microsatellite techniques in Atlantic salmon, and apply these to: i. a comparison of levels of microsatellite variation in farmed Tasmanian salmon and the parent stock in Nova Scotia (original objective 1) and ii. locating a male-sex (Ychromosone) marker for broodstock management

Final report

ISBN: 0 643 06187 8
Author: Nicholas Elliott
Final Report • 2017-09-29 • 4.10 MB
1995-080-DLD.pdf

Summary

The aquaculture of Atlantic salmon began in Australia in the mid-1960' s with an importation of ova from Canada to New South Wales. Anecdotal evidence suggested that the Australian population went through a severe bottleneck event during the early years due to poor survival and subsequent small broodstock numbers. The population, however, survived and numbers increased such that a few hundred broodstock were available each year from the early 1970' s. The now flourishing Tasmanian Atlantic salmon indust1y was founded from the New South Wales population in the mid 1980's. About 115 000, 180 000 and 275 000 ova were brought to Tasmania in 1984, 1985 and 1986, respectively. The Tasmanian industry now produces over 7 000 tonnes annually, with the majority of smolt being supplied by the Salmon Enterprises of Tasmania Pty Ltd (SAL TAS) commercial hatchery. SALT AS has for the past ten years maintained the Tasmanian broodstock, using several hundred females and males from two-year classes to produce each year's supply of smolt.

An important task for hatchery managers is the maintenance of genetic variation in their broodstock, and molecular geneticists can accurately assess genetic variation and detect meaningful changes. In recent years a new class of DNA marker - microsatellites - has shown promise for studies of genetic variation, progeny testing, genome mapping and quantitative trait loci (trait markers). We constructed an Atlantic salmon genomic DNA library which contains over 9 000 clones and several hundred potential microsatellite DNA markers. The DNA sequences of 24 of these have been determined and primers developed for eight markers for use in genetic studies. Three of these, plus five obtained from overseas contacts, were used to examine genetic variation in the Tasmanian population. As these markers were applied to the same individual fish that had earlier been examined for allozyme and mitochondrial DNA (mtDNA) variation, the relative abilities of these three techniques to detect genetic changes in hatchery populations could be assessed.

The genetic variation present in the Tasmanian and Canadian populations in 1993 was compared using these three molecular genetic methods - allozymes, mitochondrial DNA and nuclear DNA microsatellites. Some small but statistically significant allele frequency differences between the two populations were observed for one of seven polymorphic allozyme loci and for mitochondrial DNA haplotypes. However, there was no evidence of reduced genetic variability in the Tasmanian population. There were small but significant allele frequency differences observed at four of the eight microsatellite loci, and this analysis did show evidence of a small overall loss of genetic variation (loss of alleles and heterozygosity) in the Tasmanian population.

To investigate this suggested loss of genetic variation, and whether this occurred since the introduction to Tasmania or, as anecdotally suggested, during the early years of domestication in New South Wales, the genetic variation (microsatellites and allozymes) present in the Tasmanian and New South Wales populations in 1997 was then compared with that in the two previous samples.

Industry
PROJECT NUMBER • 2011-771
PROJECT STATUS:
COMPLETED

Seafood CRC: genetic selection for Amoebic Gill Disease (AGD) resilience in the Tasmanian Atlantic salmon (Salmo salar) breeding program

Amoebic gill disease (AGD) continues to have a significant economic impact upon production of sea-farmed Atlantic salmon in Tasmania. Reducing mortality is economically important for the fish farmer and is equally important from an animal welfare perspective. The process of freshwater bathing...
ORGANISATION:
CSIRO Oceans and Atmosphere Hobart

BCA: DNA microsatellite variation in Atlantic salmon

Project number: 1995-080.80
Project Status:
Completed
Budget expenditure: $5,272.01
Principal Investigator: Patrick Hone
Organisation: CSIRO Oceans and Atmosphere Hobart
Project start/end date: 26 Sep 2001 - 28 Sep 2001
Contact:
FRDC

Objectives

1. To develop DNA microsatellite techniques in Atlantic salmon, and apply these to: i. a comparison of levels of microsatellite variation in farmed Tasmanian salmon and the parent stock in Nova Scotia (original objective 1) and ii. locating a male-sex (Ychromosone) marker for broodstock management

Final report

Final Report • 177.96 KB
1995-080.80-DLD.pdf

Summary

This report carries out ex-post cost/benefit analysis on three Tasmania Atlantic salmon research projects carried out by CSIRO Marine Research, Hobart.

The three projects are analysed together as all are concerned with examining genetic diversity in Tasmanian Atlantic salmon. In addition to the issue of genetic diversity, two of the projects also attempted to develop a molecular genetic Y-chromosome marker in Atlantic salmon.

Development of the aquaculture capability of the brown tiger prawn (Penaeus esculentus)

Project number: 1996-302
Project Status:
Completed
Budget expenditure: $496,231.00
Principal Investigator: Peter Crocos
Organisation: CSIRO Oceans and Atmosphere Hobart
Project start/end date: 29 Dec 1996 - 30 May 2001
Contact:
FRDC

Objectives

1. To assess and develop the aquaculture potential of P. esculentus by:
2. Quantifying the growth performance under commercial farm culture conditions.
3. Developing a cost-effective diet for optimal growth.
4. Quantifying the potential for spawning of broodstock without ablation.
5. Closing the life cycle to provide for future selective breeding.

Final report

ISBN: 06343062424
Author: Peter Crocos
Final Report • 2000-05-28 • 3.98 MB
1996-302-DLD.pdf

Summary

In order to provide for future growth of the Australian prawn aquaculture industry, there is a need to take a longer-term strategic view and develop production technology for alternative species that can support an export market but do not have the intense overseas competition of the current major species, P. monodon. This research project addresses the need for feasibility studies on farm production of an additional species that could be profitable in new or existing markets.

The strategic objective is to build on our current information to develop and test techniques for growing P. esculentus in a farm environment, and to quantify the commercial suitability of this species. The project is structured to demonstrate the growout and market potential of the species in pilot trials, to further develop and test an appropriate growout diet, to develop closed life cycle reproduction for future selective breeding and to evaluate the cost benefit of production of P. esculentus. The project commences the development of technology and assesses the potential for P. esculentus to become an additional species for Australian farm production.

Industry
PROJECT NUMBER • 2000-224
PROJECT STATUS:
COMPLETED

Atlantic Salmon Aquaculture Subprogram: molecular genetic tools for the Tasmanian Atlantic salmon industry – development and application

Archived DNA was successfully extracted from 30-year-old dried scales collected in 1971 and 1972 from wild Atlantic salmon from the River Philip, Canada. Genetic variation was assessed at 11 nuclear DNA microsatellite loci (three tetra- and eight di-nucleotide repeats) in two samples from the River...
ORGANISATION:
CSIRO Oceans and Atmosphere Hobart
Industry
PROJECT NUMBER • 1996-347
PROJECT STATUS:
COMPLETED

Identification of a Y-chromosome marker in Atlantic salmon (extension to FRDC 95/80)

The aquaculture of Atlantic salmon began in Australia in the mid-1960' s with an importation of ova from Canada to New South Wales. Anecdotal evidence suggested that the Australian population went through a severe bottleneck event during the early years due to poor survival and subsequent small...
ORGANISATION:
CSIRO Oceans and Atmosphere Hobart