17 results
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PROJECT NUMBER • 2010-778
PROJECT STATUS:
COMPLETED

SCRC: Optimising harvest practices for Yellowtail Kingfish

Farmed Yellowtail Kingfish (YTK) is considered to be a premium quality fish, ideal for sashimi and sushi, dishes comprised of raw fish. To be acceptable for such delicacies the flesh has to be oily, firm and slightly translucent, with a fine creamy texture and a delicate flavour. The price received,...
ORGANISATION:
Flinders University
Industry
PROJECT NUMBER • 2008-758
PROJECT STATUS:
COMPLETED

Seafood CRC: Development of a genetic management and improvement strategy for Australian cultured Barramundi

The aim of this study was to review existing Barramundi-related genetic knowledge to identify relevant research and where the R&D gaps preventing instigation of Barramundi breeding programs presently exist. They identified and prioritised the research and steps that need to be taken to establish...
ORGANISATION:
Flinders University
SPECIES
Environment
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PROJECT NUMBER • 2011-758
PROJECT STATUS:
COMPLETED

SCRC: Developing efficient diagnostic tools for assessing resistance to viral infection in abalone and oysters

The project examined the genetic basis of antiviral resistance in oysters and abalone in response to the significant disease threats posed by oyster and abalone herpesviruses. Laboratory-based systems for the growth and assay of such viruses are problematic. The project undertook to measure the...
ORGANISATION:
Flinders University
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PROJECT NUMBER • 2012-714
PROJECT STATUS:
COMPLETED

SCRC: PDRS: Use of next generation DNA technologies for revealing the genetic impact of fisheries restocking and ranching

Several initiatives by the Australian Seafood CRC’s Future Harvest theme involve some form of stocking or enhancement of fisheries. In Western Australia, populations of Roe’s Abalone (Haliotis roei) are currently being restocked after the occurrence of a catastrophic mortality event,...
ORGANISATION:
Flinders University

SCRC: PhD: Improvement of abalone nutrition with macroalgae addition

Project number: 2011-751
Project Status:
Completed
Budget expenditure: $0.00
Principal Investigator: Jian Qin
Organisation: Flinders University
Project start/end date: 14 Jan 2012 - 29 Jun 2014
:

Need

The Australian Abalone Growers’ Association met in September 2009 and identified their priority areas for research. Their two highest priorities were: 1. Identification of nutritional requirements for abalone at different life stages; and 2. Identification of nutritional requirements for abalone for different seasons. Thus, this is clearly an area requiring research and collaboration with abalone growers. Therefore this project will contribute directly towards two of the industry’s identified priorities through enhancing the information generated by 2010/736.

This project lies directly within the A and B of Seafood Theme outcomes. In particular, this project addresses outcomes 1 (Academic researchers with specialist skills and knowledge specific to the seafood industry continue to be available to provide research and technical capacity to the Australian seafood industry) and 2 (CRC industry participants have the necessary skills to implement CRC research results for the use in the aquaculture and wild harvest businesses).

SCRC: PhD Extension : RNA interference (RNAi) as a means to control Neoparamoeba perurans, the causative agent of amoebic gill disease (AGD). (Student: Paula Lima)

Project number: 2012-716
Project Status:
Completed
Budget expenditure: $0.00
Principal Investigator: James O. Harris
Organisation: Flinders University
Project start/end date: 14 Mar 2012 - 13 Mar 2013
:

Need

Paula Lima began her PhD within project 2007/750 ‘AGD Vaccine Phase III: Sea-based trials, refinement and commercialisation’. Paula began in 2009 and her full stipend, travel and operating funds were paid out of this project. Paula was adopted into the Seafood CRC in late 2009, with no further financial support for her stipend or operating expenses. In 2010 at a meeting of the project’s Management Advisory Committee (MAC), a decision was made to redirect research as it was apparent that the vaccine was not a commercial reality. This occurred one full year into her PhD. This had an effect on Paula’s PhD which was subsequently changed to investigating the use of RNAi as either a research tool and/or a control strategy for AGD. This meant that the entire 1st year’s work was made redundant and Paula had to effectively ‘start over’. Paula has effectively been working in her new area for almost two years. However, the project which funded her stipend is no longer operating. Therefore, Paula still has effectively one year of her PhD left to run. For this reason we sought support from the Seafood CRC to enable Paula to complete her research. This application has the full support of Adam Main, the CEO of the Tasmanian Salmonid Growers' Association (TSGA).

Final report

ISBN: 978-1-925982-30-5
Author: Paula Cristina Walger de Camargo Lima
Final Report • 2013-03-14 • 2.50 MB
2012-716-DLD-PhD.pdf

Summary

RNA interference has emerged as a powerful tool for rapid analysis of gene function in non-model organisms and has the potential to identify candidate targets for interventions against diseases of economic importance to aquaculture. The main purpose of the study was to use functional and comparative genomics approaches to investigate whether the RNAi mechanism has been retained or lost in species from the Neoparamoeba genus.

The results provide strong evidence that both infective and non-infective species from Neoparamoeba spp. have evolutionarily retained key genes involved not only in the canonical RNAi mechanism, but also in the mirNA and PiRNA pathways. Despite being promising, these findings are still preliminary and the reality of applying RNAi technology to develop new treatment strategies against AGD still needs further effort.

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