23 results
Industry
PROJECT NUMBER • 2008-904
PROJECT STATUS:
COMPLETED

Seafood CRC: benefit-cost analysis of marker assisted selection in Australian aquaculture species

This project relates to the research strategies developed by the CRC from projects that have scoped selective breeding for abalone, Yellowtail Kingfish and Barramundi. There is potential that genetic marker assisted selection (MAS) techniques could greatly speed the genetic progress in these...
ORGANISATION:
Flinders University
Blank
PROJECT NUMBER • 2010-725
PROJECT STATUS:
COMPLETED

SCRC: PhD 6.06 – Capturing and maintaining genetic variation when initiating selective breeding programs for aquaculture

Barramundi is naturally a mass spawning species, which can be induced to spawn in captivity under conditions that attempt to replicate the natural environment. The Australian Barramundi industry has on hand a large number of mature broodstock that are currently supplying the grow-out market,...
ORGANISATION:
Flinders University
Industry
Industry
PROJECT NUMBER • 2008-903
PROJECT STATUS:
COMPLETED

Seafood CRC: Understanding Yellowtail Kingfish

This broad ranging project took a subproject approach to examine several issues that ultimately relate to product yield and quality attributes of farmed Yellowtail Kingfish (YTK) grown in sea cages. Within this project, the performance (survival and growth) of fish stocked into sea cages was...
ORGANISATION:
Flinders University
Blank
PROJECT NUMBER • 2010-778
PROJECT STATUS:
COMPLETED

SCRC: Optimising harvest practices for Yellowtail Kingfish

Farmed Yellowtail Kingfish (YTK) is considered to be a premium quality fish, ideal for sashimi and sushi, dishes comprised of raw fish. To be acceptable for such delicacies the flesh has to be oily, firm and slightly translucent, with a fine creamy texture and a delicate flavour. The price received,...
ORGANISATION:
Flinders University
Blank
Industry
Industry
PROJECT NUMBER • 1994-081
PROJECT STATUS:
COMPLETED

Experimental production of tetraploid oysters for use as broodstock for commercial hatchery production of triploids

Triploids (3n) are organisms with three sets of chromosomes, while the normal diploid (2n) organisms have only two sets. Over the past two decades, triploids have been studied in more than 20 species of molluscs. The main interests of those studies have primarily focused on their sterility and...
ORGANISATION:
Flinders University

Evaluating the Southern Bluefin Tuna cell lines as a platform for testing the effectiveness of antioxidants in preserving flesh quality

Project number: 2007-221
Project Status:
Completed
Budget expenditure: $353,279.00
Principal Investigator: Kathy Schuller
Organisation: Flinders University
Project start/end date: 13 Jul 2007 - 30 Jun 2010
:

Need

The vast majority of the Southern Bluefin Tuna (SBT) farmed in South Australia is sold fresh for sashimi production in Japan. Premium prices are obtained for sashimi grade tuna with a high fat content. Unfortunately, fatty fish tissues with their high polyunsaturated fatty acid (PUFA) content are prone to oxidation. This results in poor flesh quality, reduced shelf life and significant trimming of the tuna carcass at the market.

FRDC Project No. 2004/209 investigated the impact of dietary supplements on the flesh quality of farmed SBT. Specifically, it investigated the impact of the dietary antioxidants vitamins C and E and the mineral selenium. Project No. 2004/209 showed that these supplements can extend the shelf life of muscle samples taken from farmed SBT fed diets containing higher than normal concentrations of the antioxidants.

Although Project No. 2004/209 has been very successful, performing diet trials with live SBT is expensive and logistically difficult. It is also time-consuming and the statistical power of the experiments is limited by the fact that it is economically unfeasible to have multiple replicate sea-cages for each dietary treatment. As a result of these constraints, experiments must be repeated over several years to obtain statistically valid results.

Recently, we produced the world’s first SBT cell line and to our knowledge the first cell line for any tuna species. The cell line has the potential to reduce the number of experiments with live SBT and to greatly increase the number of dietary supplements that can be screened in a given period of time. With the cell line, it takes only one week to screen 24 different supplements with 3-4 replicates of each. This compares with years for similar experiments with live SBT.

This project will evaluate the cell line as a platform for testing dietary antioxidant supplements for SBT.

Objectives

1. To adapt existing assays for antioxidant potency from mammalian cell culture systems for use with primary fish cell cultures and immortal fish cell lines.
2. To use the adapted assays with primary fish cell cultures and immortal fish cell lines to screen a broad range of concentrations and combinations of traditional and novel antioxidants for their ability to inhibit lipid oxidation
3. To rank the antioxidants with respect to their antioxidant potency relative to vitamin E, the ‘industry standard’ antioxidant
4. To quantify how the antioxidant concentrations that are effective in primary fish cell cultures and immortal fish cell lines relate to the antioxidant concentrations that need to be added to aquafeeds
5. To undertake a cost benefit analysis of the various antioxidants and antioxidant combinations with respect to their eventual incorporation into feeds
6. To investigate the impact of dietary antioxidants on cellular antioxidants and antioxidant enzymes in primary fish cell cultures and immortal fish cell lines
7. To recommend the best concentrations and combinations of antioxidants to be added to southern bluefin tuna and yellowtail kingfish feeds to maximize fish health and flesh quality

Final report

ISBN: 978-0-646-54976-7
Author: Kathryn Schuller
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