8 results

SCRC: Visiting Expert Application: Dr Doug Tocher

Project number: 2009-740
Project Status:
Completed
Budget expenditure: $0.00
Principal Investigator: Kathy Schuller
Organisation: Flinders University
Project start/end date: 25 May 2009 - 30 Nov 2009
Contact:
FRDC

Need

Dr Douglas Tocher from the University of Stirling in Scotland is a world-renowned expert in both the practical aspects and the molecular biology of lipid nutrition in fish. He will be visiting Sydney in September 2009 to speak at the Oils and Fats conference. This is an excellent opportunity to have him visit Adelaide and Port Lincoln researchers and industry personnel to discuss possible international collaborative projects in finfish lipid nutrition.

Final report

ISBN: 978-1-925982-13-8
Author: Dr Kathy Schuller
Final Report • 2009-12-01 • 108.94 KB
2009-740-DLD.pdf

Summary

This visiting expert grant allowed a visit to South Australia by Dr Douglas Tocher. Dr Tocher is based at the Institute of Aquaculture at Stirling University in Scotland and he is internationally-recognised for his work as a research scientist in the area of finfish lipid and fatty acid nutrition. The objectives of his visit were to exchange information about the most recent developments in finfish lipid and fatty acid nutrition in Europe and Australia and to hold a workshop to develop international collaborative research projects between the Seafood CRC and the Institute of Aquaculture at Stirling University.

Dr Tocher met for technical discussions with research providers at SARDI Aquatic Sciences, Adelaide University and Flinders University. He also visited the Lincoln Marine Science Centre in Port Lincoln. The culmination of Dr Tocher's visit was a symposium on finfish lipid and fatty acid nutrition and the human health implications held at Flinders University on Thursday October 8, 2009. The symposium was attended by approximately 25 researchers from Ridley Aquafeed, the Seafood CRC, Flinders University, University of Adelaide, the Royal Adelaide Hospital, SARDI and Deakin University.

The symposium was followed by a workshop to discuss possible future collaborative research projects in the area of finfish lipid nutrition. The workshop resulted in a cluster of proposed Seafood CRC Honours projects adding value to the feeding trials planned within "Sustainable Feeds and Feed Management for Yellowtail Kingfish" and "Understanding Yellowtail Kingfish".

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PROJECT NUMBER • 2012-714
PROJECT STATUS:
COMPLETED

SCRC: PDRS: Use of next generation DNA technologies for revealing the genetic impact of fisheries restocking and ranching

Several initiatives by the Australian Seafood CRC’s Future Harvest theme involve some form of stocking or enhancement of fisheries. In Western Australia, populations of Roe’s Abalone (Haliotis roei) are currently being restocked after the occurrence of a catastrophic mortality event,...
ORGANISATION:
Flinders University
Environment
Industry

SCRC: SCRC Honours Scholarship: A novel method for producing sterile male fish and shellfish (Student - Andrew Schofield)

Project number: 2010-759
Project Status:
Completed
Budget expenditure: $0.00
Principal Investigator: Kathy Schuller
Organisation: Flinders University
Project start/end date: 14 Sep 2010 - 30 Nov 2011
Contact:
FRDC

Final report

ISBN: 978-1-925982-93-0
Author: Andrew Scholefield
Final Report • 2011-12-01 • 2.74 MB
2010-759-DLD-Hons.pdf

Summary

This study investigated the distribution of the expression of selected members of the peroxiredoxin (Prx) and glutathione peroxidase (GPx) antioxidant enzyme families in YTK (Seriola lalandi), an important finfish aquaculture species in South Australia.

The purpose of investigating the expression of these enzymes was to determine if they played a role in male fertility in fish, as both these families play a role in male fertility in mammals and individual members of these protein families were shown to be highly conserved between mammals and fish.

SCRC: SCRC Honours Scholarship H3.1– Impact of commercial hatchery practices on the contribution of broodstock to offspring and genetic diversity in the Yellowtail Kingfish breeding program (Dr Alex Safari: Student Daniel Tan)

Project number: 2009-763
Project Status:
Completed
Budget expenditure: $0.00
Principal Investigator: Alex Safari
Organisation: Flinders University
Project start/end date: 30 Sep 2009 - 29 Sep 2010
Contact:
FRDC

Final report

ISBN: 978-1-925982-29-9
Author: Daniel Tan
Final Report • 2010-09-30 • 342.44 KB
2009-763-DLD-Hons.pdf

Summary

Using small numbers of broodstock for breeding often leads to random drift in gene frequencies between generations. Effective population size (Ne) is used as an indicator to assess the rate of inbreeding and loss of genetic variation in small populations. In this study we used six microsatellites, coupled with DNA parentage analyses, to determine the relative contributions from two commercial Yellowtail Kingfish hatchery broodstock groups over four pooled spawning events (two for each group).

Initial broodstock contributions from the dams was found to be highly skewed, with one dominant dam contributing more than 70% of the offspring (1-day post hatch, dph) in three of the pooled spawning events, while two dams contributed fairly equally to the offspring in the fourth pooled spawning event. Two sires were found to be contributing more that 78% of the offspring in one pooled spawning event, while contributions between the sires were fairly equal in the other three spawning events. The student then examined the differential survival of family size through the hatchery production (from 1 to 25 dph) during which time mortality averaged 80%. Parentage analyses indicated that through the process some maternal and paternal contributions that had been initially represented in the 1-dph offspring 3 were lost. There were also significant changes in the frequencies of progeny from individual dams and sires from day 1 to day 25.

These results indicate that hatchery breeding practices and high mortality during early larvae culture would result in poor retention of genetic variability across generations. With the actual Ne being less than half of the census Ne inbreeding rates would be relatively high if the current broodstock population was closed and future broodstock were derived from existing hatchery systems.

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