13 results

Aquatic Animal Health Subprogram: establishment of a national aquatic animal health diagnostic network

Project number: 2005-621
Project Status:
Completed
Budget expenditure: $124,247.70
Principal Investigator: Richard Whittington
Organisation: University of Sydney (USYD)
Project start/end date: 30 Mar 2005 - 5 Mar 2007
:

Need

The lack of many recognised serious diseases is perceived as one of Australian aquaculture’s prime competitive advantages to meet future global demand. Maintenance of this high health status through initiatives which reduce the risk of disease incursions and facilitate early detection and response to emerging disease problems is seen as critical to continuing industry expansion. The range of commercially significant aquatic animal species, and their diseases, is increasing steadily. It is clear that, due to limited resources, diagnostic laboratories cannot develop proficiency in the diagnosis of all significant diseases, for example those listed in the Australian National List of Reportable Diseases of Aquatic Animals. Appropriately, State laboratories, in support of local industries, concern themselves with local aquatic animal species and their significant diseases and have developed expertise in those areas. Rather than duplicate this effort, AAHL’s involvement and expertise focuses on exotic diseases of concern to Australia, as well as new or emerging endemic diseases. As a consequence, expertise in specific diseases has developed in different laboratories throughout the country. To take advantage of this development, to ensure that expertise in different diseases is available Australia-wide, and to create a consistent system of aquatic animal disease diagnosis and reporting, it is proposed that a national network of laboratories should be established for the diagnosis and monitoring of aquatic animal diseases underpinned by a formal quality assurance program. Through a consultation process, uniform data standards and reporting formats need to be developed and adopted by all jurisdictions. Standard diagnostic tests and operating procedures also need to be developed and subsequently adopted by laboratories within the network. Thus this project is concerned with the establishment of the network and commencement of activities, including proficiency tests (“ring tests”) designed to assist laboratories in further developing their diagnostic capabilities and/or to allow demonstration that performance of a particular test is at a nationally accepted standard, using Australian and New Zealand Standard Diagnostic Procedures (ANZSDPs). In this way confidence of stakeholders in the quality of diagnosis provided is increased. The project is in complete alignment with AQUAPLAN 2005-2010.

Objectives

1. Make recommendations on the structure and function of the network of receival and reference laboratories
2. Establish a network for aquatic animal disease diagnosis.
3. Facilitate transfer of knowledge and technology in aquatic animal diagnostics.
4. Develop a model for national laboratory proficiency (ring) testing as a mechanism to enhance the proficiency of the established diagnostic network.

Final report

ISBN: 1-86487-8770
Author: Richard Whittington

People development program: Aquatic animal health training scheme - Visiting Expert Dr Teruo Miyazaki

Project number: 2009-315.23
Project Status:
Completed
Budget expenditure: $10,000.00
Principal Investigator: Richard Whittington
Organisation: University of Sydney (USYD)
Project start/end date: 30 Jun 2012 - 29 Jun 2013
:

Need

Viral diseases are among the most important of all health issues in aquaculture and fisheries. Their rapid and accurate recognition is central to the prevention of spread and the control of emerging diseases. Morphological diagnosis based on gross pathology, histopathology and electron microscopy remain vital in the diagnostic process, and are essential to complement accurate molecular aetiolgical diagnosis. Practitioners of morphological diagnosis are becoming scarce, and this application enables knowledge transfer from one of the world's foremost experts, Dr Teruo Miyazaki from Japan. This knowledge transfer requires a hand-on approach in a small group setting, using a multiheaded microscope with plenty of opportunity for discussion. for this reason a face-to-face visit is essential. Improving aquatic animal health diagnostic capacity is a priority for FRDC specifically to prevent and manage disease incursions, and to develop diagnostic procedures and techniques to rapidly detect and identify pathogens. This is also a priority for state jurisdictions.

In addition to viral diseases, Dr Miyazaki has career long expertise in general fish pathology, so his visit presents diverse opportunities for continuing education of Australian aquatic animal health diagnosticians.

Objectives

1. 1. Facilitate the visit to Australia of Dr Teruo Miyazaki for knowledge transfer to aquatic animal disease diagnosticians and industry
Environment

Aquatic Animal Health Subprogram: surveys of ornamental fish for pathogens of quarantine significance

Project number: 2009-044
Project Status:
Completed
Budget expenditure: $453,137.00
Principal Investigator: Joy Becker
Organisation: University of Sydney (USYD)
Project start/end date: 30 Jun 2009 - 29 Mar 2013
:

Need

In project FRDC2007/007 and previous studies it was determined that ornamental fish entering Australia may carry pathogens of quarantine concern, specifically gourami iridovirus (GIV) and cyprinid herpesvirus 2 (CyHV2). Ornamental fish are imported under a policy based on a formal Import Risk Assessment (IRA). On the 11/09/08 Biosecurity Australia announced the formal commencement of an Import Risk Analysis (IRA) under the regulated IRA process to review Australia’s freshwater ornamental finfish policy with respect to quarantine risks associated with gourami iridovirus (GIV). Australia has imported a large number of gouramis for many decades. The 1999 IRA considered several species of gouramis and concluded that specific risk management measures were required for these species due to biosecurity risk posed by iridoviruses, including GIV. Australia’s quarantine measures include that gouramis are held in an export premises for a minimum 14 day period prior to export, health certification stating that they are sourced from populations with no known significant clinical disease in the last six months, and that the fish are held in post-arrival quarantine for a minimum of 14 days. These are key features which need to be reviewed. Additional scientific data would enhance the review.

A second impact is that the developing Australian ornamental fish aquaculture industry may be at risk due to introduced pathogens. This is of particular relevance for goldfish, where domestic breeders claim that their stock succumb to diseases such as CyHV2 disease when brought into contact with imported goldfish in wholesale and retail premises. This disease agent was also specifically addressed in the 1999 IRA.

There is need to determine whether GIV and CyHV2 are in fact entering Australia despite quarantine practices, and further, to determine whether either virus is already established in farmed or wild ornamental fish in Australia.

Objectives

1. To determine whether GIV is entering Australia despite quarantine practices
2. To determine whether CyHV2 is entering Australia despite quarantine practices
3. To determine whether GIV is already established in farmed gourami in Australia
4. To determine whether CyHV2 is already established in farmed goldfish in Australia
5. To determine whether GIV is already established in wild gourami in Australia
6. To determine whether CyHV2 is already established in wild goldfish in Australia
7. To determine whether domestic goldfish free of CyHV2 succumb to disease when cohabitated with imported goldfish carrying CyHV2
8. To extend the findings of this study to the ornamental fish sector in Australia and provide information for use by DAFF

Final report

ISBN: 978-1-74210-314-3
Author: Joy Becker

Aquatic Animal Health Subprogram: Pacific oyster mortality syndrome (POMS) - risk mitigation, epidemiology and OsHV-1 biology

Project number: 2012-032
Project Status:
Completed
Budget expenditure: $783,045.00
Principal Investigator: Richard Whittington
Organisation: University of Sydney (USYD)
Project start/end date: 18 Jun 2012 - 6 Sep 2015
:

Need

There is a disturbing pattern of diseases in commercial molluscs nationally. They have required a succession of government/industry responses, with no clear solutions:QX disease, Sydney rock oysters, NSW and QLD; NSW; Pacific oyster mortality syndrome, NSW; Abalone viral ganglioneuritis, VIC; Oyster oedema disease, pearl oysters, WA; Winter mortality, Sydney rock oyster, NSW.

Economic impacts have been substantial or devastating. Wild fisheries and aquaculture have been impacted. In NSW, the primary impact of QX disease led to replacement of Sydney rock oysters by triploid Pacific oysters to reestablish the industry in some estuaries, but this is now threatened by POMS.

In every case the new disease has spread. It has not been possible to devise an intervention strategy that would halt disease spread or ensure the recovery of the industry. Investigating the behaviour of POMS during its recrudescence in summer 2011/2012 in FRDC project 2011-053 afforded a unique insight into the disease, and these observations need to be extended over time to identify factors which may be used to reduce the impact of the infection.

This project seeks to address 6 specific research priorities identified by FRDC and will concurrently investigate the effect of host, environment and husbandry factors on POMS prevalence and mortality rate in Pacific oysters with the objective of discovering aspects of epidemiology which can be manipulated by oyster growers. If POMS spreads beyond its current limited distribution in NSW, commercial scale production of Pacific oysters in the face of POMS will be essential for the viability of the industry pending development of technical solutions such as genetically resistant lines.

FRDC strategic R&D theme 1 - biosecurity and aquatic animal health, and Aquatic Animal Health Subprogram priority - Nature of disease and host-pathogen interaction - immunology of aquatic invertebrates.

Objectives

1. To determine/confirm the identity of the one or more variant(s) of Ostreid herpesvirus associated with the recent outbreaks of POMS
2. To determine the mechanism(s) of transmission of disease
3. To determine the major risk factors that contribute to precipitation of disease outbreaks thereby identifying potential risk-mitigation management practices
4. To identify the natural reservoir(s) for the virus
5. To determine the stability of the virus in the environment
6. To identify physical and chemical means for viral inactivation
7. To develop an infecitivity model for POMS suitable for selection of resistant oysters and pathogenesis/environmental research
8. To address future shortages of technical expertise through the training and supervision of at least 1 PhD student

Project products

Journal • 2013-12-17

Summary

Abstract:

Mortality of farmed triploid Pacific oysters (Crassostrea gigas) associated with Ostreid herpesvirus-1 (OsHV-1) was first recorded in Australia in the Georges River/Botany Bay estuary (New South Wales) in late 2010. Two years later, the first sign of possible inter-estuarine spread was observed when commercial triploid Pacific oysters in the Hawkesbury River estuary, located 50 km north of Botany Bay, were affected by mass mortality.

Journal • 2015-03-09

Summary

Abstract:

In Australia, the spread of the ostreid herpesvirus-1 microvariant (OsHV-1 μVar) threatens the Pacific oyster industry. There is an urgent need to develop an experimental infection model in order to study the pathogenesis of the virus under controlled laboratory conditions. The present study constitutes the first attempt to use archived frozen oysters as a source of inoculum, based on the Australian OsHV-1μVar strain.

Journal • 2014-11-21

Summary

Abstract:

 

The microvariant genotype of Ostreid herpesvirus-1 (OsHV-1 μVar) has severely disrupted the production of Pacific oysters Crassostrea gigas in Europe, New Zealand, and Australia since its first detection in France in 2008. The disease occurs in the warmer months, recurs annually, and requires new management strategies. Larvae and spat are the most susceptible life history stages, which poses a threat to hatchery production.

 

Journal • 2015-01-06

Summary

Abstract:

Management of mass mortality events associated with Ostreid herpesvirus-1 microvariant (OsHV-1 μVar) is vital for aquaculture of Crassostrea gigas. As a consequence, the understanding of transmission mechanisms and risk factors enabling husbandry solutions to be developed constitutes an international research priority. In this context, a longitudinal intervention study was set up in Woolooware Bay, Australia, during summer in 2012–2013.

Journal • 2013-07-29

Summary

Abstract:

In 2010 Ostreid herpesvirus-1 (OsHV-1) was detected in Australia and had a disastrous impact on Pacific oyster Crassostrea gigas aquaculture and coastal communities. The acronym POMS (Pacific Oyster Mortality Syndrome) was created in Australia to refer to mass mortalities due to OsHV-1. While management of this disease mainly involves active surveillance, rigorous biosecurity protocols and mollusc breeding  programs targeting production of resistant animals, the effects of aquaculture practices on mortality outbreaks are still poorly understood. The present study aimed to determine the effect of growing heights on OsHV-1 associated mortality in C. gigas in Woolooware Bay (Australia) during the summer 2011/2012.

Journal • 2014-10-05

Summary

Abstract:

Ostreid herpesvirus-1 (OsHV-1) is responsible for massive mortality events in commercially farmed Pacific oysters (Crassostrea gigas) in Australia, New Zealand, Europe and the USA. Economic losses have been severe in many countries since 2008, associated with a strain known as OsHV-1µ-var. Despite intensive studies of the virus itself, there is almost no information on its detection in natural seawater, how it is spread over wide geographic distance in water or on how it is transmitted from oyster to oyster via seawater.

Environment
PROJECT NUMBER • 2016-011
PROJECT STATUS:
COMPLETED

Aquatic Animal Health and Biosecurity Subprogram: Disinfection measures to support biosecurity for infectious spleen and kidney necrosis virus (ISKNV) at aquaculture facilities

Megalocytiviruses are a group of closely related viruses that cause mass mortalities in both marine and freshwater aquaculture. They are of global importance as they are listed by the World Animal Health Organization (OIE). They are difficult to control as they infect over 125 fish species and...
ORGANISATION:
University of Sydney (USYD)

Aquatic Animal Health Subprogram: Tools for investigation of the nodavirus carrier state in marine, euryhaline and freshwater fish and control of NNV through integrated management

Project number: 2008-041
Project Status:
Completed
Budget expenditure: $463,365.00
Principal Investigator: Richard Whittington
Organisation: University of Sydney (USYD)
Project start/end date: 31 Mar 2009 - 29 Mar 2012
:

Need

This project relates directly to the FRDC VNN Research and Development Plan, to facilitate industry profitability, sustainability, growth and development. There is an overarching need to measure and then reduce the risk to fisheries and aquaculture sectors (including natural resources) associated with transfer of nodaviruses. The aquaculture industry is proactive and responsible (eg triple bottom line reporting) and wishes to manage risks based on sound science. Industry acknowledges a residual risk that cannot be controlled: virus prevalence in the wild and natural fish movements. However, there is an immediate need for industry to conduct business in the face of unknowns with respect to true disease status. While there is a need to ensure that infected, but apparently healthy, stock are not moved to areas that are considered free of the disease/disease agent, it is of fundamental importance for the sustainability of the barramundi aquaculture industry and developing species ventures such as Australian bass that stock are translocated. Current tests for determining disease status are considered inadequate, therefore biosecurity protocols in the short-term are required to address the risk of introduction of disease with water, broodstock and fomites. As new information becomes available through R&D, these protocols will be revised and improved as needed to improve biosecurity. In the meantime, protocols are needed to manage risks with incomplete information and without overburdening industry with uneconomic or unwarranted requirements. There is need for mitigation of impacts on translocation: hatchery to nursery to grow-out; hatchery to wild (eg stocking for recreational fishing); both intra-state and interstate translocations, access to overseas markets; sourcing broodstock from the wild. Financial impacts, environmental impacts and mulitsectorial impacts at level of commercial, recreational and regulatory sectors all need to be addressed.

Objectives

1. To develop and validate a real-time PCR method for the detection and identification of betanodaviruses
2. To develop and evaluate the applicability of serological tests for detection and identification of betanodaviruses
3. To transfer developed technology to Australian diagnostic laboratories
4. To provide a basis for development of a national proficiency testing scheme for the detection and identification of betanodaviruses
5. To provide recommendations for improved biosecurity protocols in relation to nodavirus infection and fish translocation

Final report

ISBN: 978-1-74210-301-3
Author: Richard Whittington

Aquatic Animal Health Subprogram: Strategic approaches to identifying pathogens of quarantine concern associated with the importation of ornamental fish

Project number: 2014-001
Project Status:
Completed
Budget expenditure: $249,836.00
Principal Investigator: Joy Becker
Organisation: University of Sydney (USYD)
Project start/end date: 15 Jun 2014 - 30 Oct 2016
:

Need

Nearly 18 million ornamental fish are imported annually under a policy based on an Import Risk Analysis published in 1999. Despite the biosecurity measures in place since 2000, there have been several incidents of exotic pathogens from ornamental fish affecting wild and farmed fish populations. These include atypical Aeromonas salmonicida, cyprinid herpesvirus 2 (CyHV2) and dwarf gourami iridovirues (DGIV). There is a need to acquire new knowledge to support policy reform as the ornamental fish industry advances and new pathogens emerge.
Project FRDC 2009/044: AAH: surveys of ornamental fish for pathogens of quarantine significance determined that ornamental fish with subclinical infections of exotic viruses were passing through quarantine undetected. This project provided scientific evidence that led Biosecurity Australia to relax the import conditions for goldfish as CyHV2 is now considered endemic. Also, DGIV was detected in all consignments of imported gouramis at an average prevalence of 19%. DGIV was detected in domestic fish populations at wholesale and retail premises and at one ornamental aquaculture facility. The project outcomes indicated that pre-export biosecurity measures (and associated health certification provided by exporting country authorities) and post-arrival quarantining of fish in Australia were insufficient to detect and prevent fish with subclinical infections of exotic pathogens from entering Australia.

There is little information available on other viral, bacterial and parasitic disease agents carried by imported ornamental fish. Specifically, there is a paucity of information available on parasites of imported fish and their potential risk to Australian industries and ecosystems. There is a need to determine whether pathogens of biosecurity significance associated with ornamental fish are entering Australia despite the import conditions.

Objectives

1. Determine if pathogens of potential biosecurity concern on the national list are entering QAPs in Australia through the trade in ornamental fish
2. Determine if current import conditions for goldfish requiring freedom from specific pathogens are being met
3. Document parasites of potential biosecurity concern that are associated with imported ornamental fish
4. Develop efficient pooled sample strategies for testing imported fish

Final report

ISBN: 978-1-74210-399-0
Author: Joy Becker

Aquatic Animal Health Subprogram: Pacific oyster mortality syndrome (POMS) - understanding biotic and abiotic environmental and husbandry effects to reduce economic losses

Project number: 2011-053
Project Status:
Completed
Budget expenditure: $130,000.00
Principal Investigator: Richard Whittington
Organisation: University of Sydney (USYD)
Project start/end date: 31 Oct 2011 - 16 Dec 2012
:

Need

There is a disturbing pattern of diseases in commercial molluscs nationally. They have required a succession of government/industry responses, with no clear solutions:QX disease, Sydney rock oysters, NSW and QLD; NSW; Pacific oyster mortality syndrome, NSW; Abalone viral ganglioneuritis, VIC; Oyster oedema disease, pearl oysters, WA; Winter mortality, Sydney rock oyster, NSW.

Economic impacts have been substantial or devastating. Wild fisheries and aquaculture have been impacted. In NSW, the primary impact of QX disease led to replacement of Sydney rock oysters by triploid Pacific oysters to reestablish the industry in some estuaries, but this is now threatened by POMS.

In every case the new disease has spread. It has not been possible to devise an intervention strategy that would halt disease spread or ensure the recovery of the industry. Investigating the behaviour of POMS during its predicted recrudescence this summer provides an opportunity to identify factors which may be used to reduce the impact of the infection.

This project seeks to investigate the effect of host, environmental and husbandry factors on POMS prevalence and mortality rate in Pacific oysters during summer 2011-autumn 2012 with the objective of discovering aspects of epidemiology which can be manipulated by oyster growers.

FRDC strategic R&D theme 1 - biosecurity and aquatic animal health, and Aquatic Animal Health Subprogram priority - Nature of disease and host-pathogen interaction - immunology of aquatic invertebrates.

Objectives

1. To correlate biotic and abiotic environmental factors with POMS occurrence in selected oyster populations

Final report

ISBN: 978-1-74210-309-9
Author: Richard Whittington
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