Abalone Growers Associations in Victoria, Tasmania and South Australia have given their support for the establishment of health monitoring programs of their farm’s stock, and are willing to contribute financially for establishing these and for ongoing monitoring. They see such programs as putting them in a unique position to to enable them to prevent disease in their stock and of meeting future market expectations in relation to health accreditation. This process is threatened by inadequate data on diseases in wild stock, and in some states by limited experience in abalone diagnosis.

Similarly the wild harvest abalone industry recognises vulnerability from a lack of knowledge of the occurrence and distribution of diseases in Australian wild stocks, in a climate where interstate translocation and trade access and quality issues are increasing. They also seek assurances on the health of farmed stock and of stock used for reseeding operations. As a result, the wild abalone sector and fisheries and animal health authorities in these states also strongly support a baseline survey of diseases present in the exploited abalone species, and development of improved surveillance capability.

The project is to acquire this background data on abalone disease, by a health survey covering the natural range of the exploited temperate abalone species. It includes the abalone aquaculture industry and the wild fishery in SA, Victoria, Tasmania, NSW and WA. The project will incorporate associated initial training, permanent presentation on collated results in accessible electronic format (CD and the subprogram’s website), and an abalone disease symposium to develop a net-work of state-based resources for on-going diagnosis, health certification and other shellfish health related functions at reasonable cost to the industry.

Development of the on-going state-based surveillance programs for aquaculture will progress in conjunction with this (without cost to the project), and with consultation with wild fisheries industries and managers, who will also benefit from improved capability for diagnostic and surveillance services.

Modelling of the Tasmanian lobster resource has indicated that loss of yield through spatial differences in growth of lobsters is greater than 25% of the TACC. Effects of fishing on egg production/recruitment and ecology also appear poorly managed spatially.

Increasing catch targets high priority areas in the strategic plans of each stakeholder. The Tasmanian Government has stated their intent to pursue growth in primary industry as a key strategic area through the “State of Growth” strategy. The project squarely targets all aspects of the University of Tasmania's “EDGE agenda”, particularly through “Engagement” with the community by delivery of a substantial economic benefit. The need for this research has been identified by the commercial and recreational lobster sectors in each strategic plan for crustacean research since the first plan was produced by the CRAG in 1996, specifically under the topics of “stock enhancement” and “translocation”.

This project will develop methods and provide information for vaccine and novel treatment development. For example, techniques for the isolation and maintenance of N. pemaquidensis are based on monoxenic cultures. This culture is highly problematic because preparations of protozoa are contaminated with bacteria. Studies to determine cell function, protein and DNA composition have been seriously compromised by the bacteria. Culture relies on the use of agar. Cell propagation and harvesting by this system is time consuming and inefficient. Development of practical systems for cell factory production of N. pemaquidensis is required. This is important for studies of cell wall composition and cell function, which require considerable biomass. There is no model of infection using protozoa derived from monoxenic or xenic cultures. This represents a major limitation, particularly when it is necessary to use controlled doses of a single strain. Current methods rely on the use of N. pemaquidensis harvested from infected fish. While this strategy meets an immediate need, long-term it cannot be justified. Development of a method to grow in vitro virulent protozoa capable of infecting fish is an essential objective. The current library of N. pemaquidensis isolates obtained from fish with AGD is small and in continuous culture for almost 10 years. There is an urgent need to re-isolate N. pemaquidensis and expand the library to ensure an adequate range of phenotypes and genotypes. Preservation of N. pemaquidensis is an essential requirement of the AGD programme as it will maintain strain integrity, a vital objective for vaccine development. The complexity of growing N. pemaquidensis has proved a major limitation to studies on AGD. A centre of expertise in the culture of N. pemaquidensis should result in guaranteed supply of organism. A reference laboratory will ensure standardisation of cultures and uniformity of research outcomes.