50,414 results

SCRC: Development of germ cell transplantation technology for the Australian aquaculture industry

Project number: 2011-730
Project Status:
Completed
Budget expenditure: $0.00
Principal Investigator: Abigail Elizur
Organisation: University of the Sunshine Coast (USC)
Project start/end date: 14 Sep 2011 - 29 Jan 2014
Contact:
FRDC

Need

Currently SBT is being bred in an expensive on-shore facility at Arno Bay, where a single tank holds a limited number of broodstock, which spawn for a limited period of time. In order to expand on the
production of SBT seed, additional facilities/tanks at the costs of millions of dollars will be required and
sourcing additional 12 year old broodstock. Therefore there is a need to look at alternative approaches
for SBT broodstock management. This proposal explores the application of a highly innovative
approach - that is the use of fish surrogates to produce SBT. By identifying the right surrogate for SBT
and developing the specific know-how with respect to optimal germ cell management, SBT seed could
be produced in a fast maturing small host. This would completely overcome the need for large,
expensive broodstock facilities and long term holding of broodstock, while ensuring a continuous
supply of SBT seed, which is much needed for larval rearing R&D and commercialization. This
application relates to the overall investment in closing of the life-cycle of SBT.

Final report

ISBN: 978-0-9804744-3-5
Authors: Abigail Elizur Erin Bubner Ido Bar Andre Smith Scott Cummins Luke Dutney and Peter Lee
Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

Final Report • 2014-05-01 • 6.61 MB
2011-730-DLD.pdf

Summary

The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.

We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.

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PROJECT NUMBER • 2009-749
PROJECT STATUS:
COMPLETED

SCRC: Improvements in Yellowtail Kingfish larval and juvenile survival and quality

This project consists of nine research projects addressing nine objectives set out in the overarching program of activity described in project 2007/707. The nine projects collectively endeavour to resolve the larval rearing, juvenile development and productivity constraints for propagated Southern...
ORGANISATION:
Clean Seas Seafood Ltd
Blank
PROJECT NUMBER • 2018-016
PROJECT STATUS:
COMPLETED

Improving data on Aboriginal and Torres Strait Islander marine resource use to inform decision-making

Through two national workshops, Indigenous community and agency representatives and researchers discussed issues around collecting, sharing and ownership of Indigenous fishing data. Challenges and opportunities were shared from all perspectives and expertise, knowledge and information came together...
ORGANISATION:
Department of Primary Industries and Regions South Australia (PIRSA)
Industry
PROJECT NUMBER • 2010-736
PROJECT STATUS:
COMPLETED

Seafood CRC: development of formulated diets for cultured abalone

The key research findings described in this project addressed the two highest research priorities identified by the Australian Abalone Grower' Association (AAGA) in 2009, prior to the commencement of this project.: Improve our understanding of the effects of seasonal water temperatures on the...
ORGANISATION:
SARDI Food Safety and Innovation
Industry
PROJECT NUMBER • 2008-756
PROJECT STATUS:
COMPLETED

Seafood CRC: increasing seedstock production of domesticated giant tiger prawns (Penaeus monodon) through improved male fertility

Given the rationale that pond systems are likely the most cost-effective system for large-scale production of Giant Tiger Prawn (Penaeus monodon) broodstock, this project aimed to determine whether pond-rearing poses a significant risk for broodstock production. The gross reproductive development of...
ORGANISATION:
CSIRO Oceans and Atmosphere Hobart
Blank
PROJECT NUMBER • 2008-907
PROJECT STATUS:
COMPLETED

SCRC: COMMUNAL PROJECT: Seafood CRC Participation in the Seafood Access Forum (SAF)

The goal of the Seafood CRC is to double the value of the Australian seafood industry to $4billion per annum by 2017 and to generate more than 2,800 jobs in rural and regional areas. Achieving this goal will require a concerted effort by the Seafood CRC, its core participants and collaborators over...
ORGANISATION:
Seafood Services Australia Ltd
Industry
PROJECT NUMBER • 2008-903
PROJECT STATUS:
COMPLETED

Seafood CRC: Understanding Yellowtail Kingfish

This broad ranging project took a subproject approach to examine several issues that ultimately relate to product yield and quality attributes of farmed Yellowtail Kingfish (YTK) grown in sea cages. Within this project, the performance (survival and growth) of fish stocked into sea cages was...
ORGANISATION:
Flinders University
Environment
PROJECT NUMBER • 2016-132
PROJECT STATUS:
COMPLETED

NCCP: Impact Costs of Carp & Expected Benefits and Costs Associated with Carp Control in the Murray Darling Basin

This project, undertaken by Agtrans Research in association with Environmental and Resource Economics (ERE) and Gillespie Economics, investigated the current and future impact costs of European Carp in Australian waterways, particularly the Murray Darling Basin (MDB), and the costs and benefits of...
ORGANISATION:
Agtrans Research
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