75,296 results
Industry
PROJECT NUMBER • 2008-903
PROJECT STATUS:
COMPLETED

Seafood CRC: Understanding Yellowtail Kingfish

This broad ranging project took a subproject approach to examine several issues that ultimately relate to product yield and quality attributes of farmed Yellowtail Kingfish (YTK) grown in sea cages. Within this project, the performance (survival and growth) of fish stocked into sea cages was...
ORGANISATION:
Flinders University
TAGS
Stock Assessment
Reproduction
Physiology
Nutrition
Marketing
SPECIES
Yellowtail Kingfish
Environment
PROJECT NUMBER • 2016-132
PROJECT STATUS:
COMPLETED

NCCP: Impact Costs of Carp & Expected Benefits and Costs Associated with Carp Control in the Murray Darling Basin

This project, undertaken by Agtrans Research in association with Environmental and Resource Economics (ERE) and Gillespie Economics, investigated the current and future impact costs of European Carp in Australian waterways, particularly the Murray Darling Basin (MDB), and the costs and benefits of...
ORGANISATION:
Agtrans Research
TAGS
Stakeholder
Modelling
Marketing
Invasive Species
Health And Safety
SPECIES
European Carp
Adoption
PROJECT NUMBER • 2008-902
PROJECT STATUS:
COMPLETED

Seafood CRC: aquaculture innovation hub

The Aquaculture Innovation Hub was a new initiative from the Seafood CRC and the Fisheries Research and Development Corporation to coordinate aquaculture research under the Seafood CRC, facilitate improved communication and assist development of new collaborative projects. The hub brought together...
ORGANISATION:
Department of Primary Industries and Regional Development (NSW)
TAGS
Training
Profitability
Innovation
Husbandry
Conference
Industry
PROJECT NUMBER • 2009-752.10
PROJECT STATUS:
COMPLETED

Seafood CRC: seafood trade expert panel (SafeFish)

SafeFish originated from the recognition by the members of the former Seafood Access Forum (SAF) (industry and government) of the need to provide coordinated, cohesive and robust technical advice to support Australian negotiators and delegations dealing with trade and market access issues related to...
ORGANISATION:
SARDI Food Safety and Innovation
TAGS
Trade
Toxin
Supply Chain
Fishing Gear
Fisheries Management
Industry
PROJECT NUMBER • 2008-717
PROJECT STATUS:
COMPLETED

Seafood CRC: improving quality of Australian Sardines through utilization of flow-ice technology

This project assessed the utility of flow-ice in preserving the freshness of Australian Sardines from the point of harvest to processing, and wholesale and retail supply chains. Trials were carried out on the Gemma Marie (White Fisheries) based at Port Lincoln, South Australia. Experiments involved...
ORGANISATION:
SARDI Food Safety and Innovation
TAGS
Value Add
Supply Chain
Resource Access And Allocation
Post Harvest
Innovation
SPECIES
Sardines

Improved diagnosis of the infectious disease epizootic haemopoietic necrosis (EHN) in salmonid fish in Australia

Project number: 1989-085
Project Status:
Completed
Budget expenditure: $150,322.00
Organisation: Department of Primary Industries and Regional Development (NSW)
Project start/end date: 28 Jun 1990 - 31 Dec 1992
Contact:
FRDC

Objectives

1. Develop fast, cheap & accurate immunodiagnostic test for EHN virus (EHNV)
for certification of disease free salmonids & distribution of EHNV in salmonid fish and redfin perch.

Final report

ISBN: 0 7305 6704 4
Author: R. J. Whittington
Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

Final Report • 1992-12-14 • 3.59 MB
1989-085-DLD.pdf

Summary

Epizootic haematopoietic necrosis vims (EHNV) was the first virus isolated from finfish in Australia. It causes outbreaks of disease in wild redfin perch (Perca fluviatilis) and also in hatchery reared rainbow trout (Onchorhynchus mykiss).

The principal aim of this project, which was to develop a rapid, cheap and accurate immunodiagnostic test for EHN vims (EHNV) infection, was achieved. The new test, an enzyme-linked immunosorbent assay (ELISA) will replace the traditional technique of vims isolation for routine diagnosis of EHNV and will be utilised as a certification test. The ELISA detects the presence of EHNV components (known as antigens) in the tissues of infected fish. The test has a sensitivity of 80%, a level which is considered to be more than adequate for evaluation of samples in fish disease diagnosis. The specificity of the ELISA is greater than 98%.

Blood samples were collected from a population of rainbow trout on a commercial hatchery known to be endemically infected with EHNV. Antibodies (substances produced by an infected fish in response to infection) specifically directed against EHNV were not detected in any of these fish. However, anti-EHNV antibody was detected in the blood of wild redfin perch from Victoria. The potential role of serology in the diagnosis of EHNV infection cannot be assessed at this time because of incomplete understanding of the behaviour of the disease in infected populations of rainbow trout and redfin perch. Further information is required on the epidemiology of EHNV in hatchery populations of rainbow trout and in wild populations of redfin perch. Acquisition of this data using the tests developed during this study is essential for the formulation of rational protocols for disease certification. In addition, further data is needed on possible differences between strains of EHNV and other iridovimses that have been isolated in Australia.

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