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Adoption

SCRC: PhD: Profiling host-parasite dynamics of AGD using molecular DNA methods – application to vaccine development, selective breeding and offshore aquaculture

Project number: 2009-757
Project Status:
Completed
Budget expenditure: $0.00
Principal Investigator: James O. Harris
Organisation: Flinders University
Project start/end date: 28 Feb 2010 - 31 May 2013
Contact:
FRDC

Need

Amoebic gill disease (AGD) research remains a high priority for the Tasmanian salmon industry. Within this framework there is a need to develop, both for research and practical reasons, non-destructive quantitative measures of AGD severity. This PhD project will develop a state of the art quantitative real time PCR (qRT-PCR) method for AGD-causing Neoparamoeba perurans. If successful this will be the first such DNA test of its type in the world for this disease. The assay will then be utilised to answer practical questions such as profiling host-parasite dynamics in vaccinated and non-vaccinated salmon prior to, during and after commercial freshwater bathing treatment, providing a rigorous measure of vaccine efficacy, and much-needed insights into the parasite loading exhibited by the different experimental salmon groups. The N. perurans DNA test will then be extended to selectively-bred salmon, correlating pathogen load with breeding values for resistance in F2 stock. This could provide a more reliable way of quantifying infection than current gill scoring methods, and will be the first time such a tool is applied to gain more precise information from a commercial salmon selective breeding program. Finally, the project will then apply the qRT-PCR test and other N. perurans molecular markers to the wider environment to address questions of population genetics, environmental reservoirs (providing much-needed information on the parasite life cycle, a prelude to in vitro culture which would benefit vaccine development), and parasite dynamics in heavily-farmed and virgin marine environments to address fundamental questions as the Tasmanian salmon industry contemplates a move towards off-shore aquaculture.
This project is a high priority for the Tasmanian salmon industry and was adopted into the CRC at its inception. This PhD project has the support of the industry. The project also has strong alignment with the industry run selective breeding program.

Environment
Environment
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PROJECT STATUS:
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The project has provided an understanding of how environmental variation has impacted on WCPF production. Chapter 1 contains the objectives of the study, background and need for the work. Chapter 2 provides a context for understanding of: a) oceanic and climatological processes which are associated...
ORGANISATION:
Spencer Gulf and West Coast Prawn Association (SGWCPA)
Industry
PROJECT NUMBER • 1985-075
PROJECT STATUS:
COMPLETED

Prawn farming pond management

The bioassay section of this study provides, for the first time, estimations of lethal and growth reducing concentrations for the major production-limiting water quality variables for P. monodon. These critical concentrations were assessed using comparable experimental facilities and procedures....
ORGANISATION:
Department of Primary Industries and Regional Development (NSW)
Environment
PROJECT NUMBER • 2016-068
PROJECT STATUS:
COMPLETED

Vulnerability of the endangered Maugean Skate population to degraded environmental conditions in Macquarie Harbour

Maugean Skate (Zearaja maugeana) are only known from two isolated estuarine systems located on the west coast of Tasmania, representing one of most restricted distributions of any elasmobranch. There is, however, uncertainty about the continued persistence of the species in one of these estuaries...
ORGANISATION:
University of Tasmania (UTAS)
SPECIES
Industry
PROJECT NUMBER • 2018-017
PROJECT STATUS:
COMPLETED

Demand Conditions and Dynamics in the Southern and Eastern Scalefish and Shark Fishery: Empirical Investigation

This final report, a collaboration between economists from CSIRO, CQU and ABARES, is the first detailed analysis of the interrelationship between fish prices on the Sydney and Melbourne fish markets. In addition, the study derived empirical estimates of the own and cross-price flexibilities for the...
ORGANISATION:
CSIRO Oceans and Atmosphere Hobart
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