341 results
Environment
Environment

Understanding Ostreid herpesvirus type 1 risk: alternative hosts and in situ hybridisation

Project number: 2018-102
Project Status:
Completed
Budget expenditure: $59,327.00
Principal Investigator: Peter Speck
Organisation: Flinders University
Project start/end date: 15 Nov 2018 - 29 Jun 2019
Contact:
FRDC
TAGS
Recreational Fishing
Mortality
Disease
Biotechnology
Biosecurity
SPECIES
Sydney Rock Oyster
Pacific Oyster
Blue Mussel

Need

To understand the risk of OsHV-1 spreading to commercial Pacific oyster growing regions, vectors need to be understood. A major gap is to understand the importance of non-OsHV-1 hosts and their role as reservoirs and in transmission of the virus. This project will provide information to better understand this risk, and to inform the status of PIRSA's ban on take of shellfish from the Port River.

Objectives

1. Implement and validate OsHV-1 in situ hybridisation assay
2. Assess OsHV-1 infection in PCR positive non-C. gigas hosts using ISH

Final report

ISBN: 978-1-925562-33-0
Authors: Peter Speck Jim Mitchell Jane Yeadon and Marty Deveney
Final Report • 2019-09-01 • 863.76 KB
2018-102-DLD.pdf

Summary

South Australia (SA) has a large edible oyster industry primarily growing Pacific oysters (Crassostrea gigas). The industry is regionally-based, an important employer and a substantial contributor to regional economies. Pacific oyster mortality syndrome (POMS) is a serious infectious disease of C. gigas caused by ostreid herpesvirus-1 microvariant (OsHV-1 microvariant). The first outbreak of OsHV-1 in Australia occurred in 2010, in the Georges River-Botany Bay and Port Hacking-Sydney Harbor estuaries in NSW. OsHV-1 was subsequently detected in the Hawkesbury River system (2013) and eastern Tasmania (2016). The production and economic impacts of these outbreaks have been substantial.
OsHV-1 infection was confirmed in the Port River, SA, in February 2018 in association with high mortality (50-90+%) of feral Pacific oysters. POMS was not identified in SA outside the control area, and surveillance has shown commercial growing areas in SA to be free of OsHV-1. Surveillance has shown that C. gigas in the Port River system has high prevalence of OsHV-1 infection.
Mussels (Mytilus spp.) share similar habitats to Pacific oysters and are common in the Port River estuary (1). Mussels are demonstrated as hosts for OsHV-1 in Ireland (2), and show some histopathological signs of disease but outbreaks in mussels are not described. We aimed to implement an OsHV-1 in situ hybridization (ISH) assay and assess OsHV-1 infection in PCR positive non-C. gigas hosts using ISH.
An in situ hybridization (ISH) test for OsHV-1 was implemented at Flinders University based on published primers for detection of the virus. ISH showed a strong signal in sections from infected C. gigas and none in uninfected C. gigas. Bivalves were collected in the Port River by SARDI, sampled and tested for OsHV-1 using PCR. Three PCR positive samples of Mytilus spp. were also positive by ISH, showing infection with OsHV-1. 
Confirmation that mussels are a host of OsHV-1 has a range of important management implications. Movement of mussels likely poses a risk for transmission of OsHV-1, so control activities designed to decrease host populations that target only Pacific oysters are unlikely to be successful; biofouling management should be general rather than targeting only Pacific oysters. Containment measures should target all bivalve species. Mussels for translocation to OsHV-1 free areas should be sourced from biosecure hatcheries and tested to provide evidence of OsHV-1 freedom.
Final Report • 2019-09-01 • 863.76 KB
2018-102-DLD.pdf

Summary

South Australia (SA) has a large edible oyster industry primarily growing Pacific oysters (Crassostrea gigas). The industry is regionally-based, an important employer and a substantial contributor to regional economies. Pacific oyster mortality syndrome (POMS) is a serious infectious disease of C. gigas caused by ostreid herpesvirus-1 microvariant (OsHV-1 microvariant). The first outbreak of OsHV-1 in Australia occurred in 2010, in the Georges River-Botany Bay and Port Hacking-Sydney Harbor estuaries in NSW. OsHV-1 was subsequently detected in the Hawkesbury River system (2013) and eastern Tasmania (2016). The production and economic impacts of these outbreaks have been substantial.
OsHV-1 infection was confirmed in the Port River, SA, in February 2018 in association with high mortality (50-90+%) of feral Pacific oysters. POMS was not identified in SA outside the control area, and surveillance has shown commercial growing areas in SA to be free of OsHV-1. Surveillance has shown that C. gigas in the Port River system has high prevalence of OsHV-1 infection.
Mussels (Mytilus spp.) share similar habitats to Pacific oysters and are common in the Port River estuary (1). Mussels are demonstrated as hosts for OsHV-1 in Ireland (2), and show some histopathological signs of disease but outbreaks in mussels are not described. We aimed to implement an OsHV-1 in situ hybridization (ISH) assay and assess OsHV-1 infection in PCR positive non-C. gigas hosts using ISH.
An in situ hybridization (ISH) test for OsHV-1 was implemented at Flinders University based on published primers for detection of the virus. ISH showed a strong signal in sections from infected C. gigas and none in uninfected C. gigas. Bivalves were collected in the Port River by SARDI, sampled and tested for OsHV-1 using PCR. Three PCR positive samples of Mytilus spp. were also positive by ISH, showing infection with OsHV-1. 
Confirmation that mussels are a host of OsHV-1 has a range of important management implications. Movement of mussels likely poses a risk for transmission of OsHV-1, so control activities designed to decrease host populations that target only Pacific oysters are unlikely to be successful; biofouling management should be general rather than targeting only Pacific oysters. Containment measures should target all bivalve species. Mussels for translocation to OsHV-1 free areas should be sourced from biosecure hatcheries and tested to provide evidence of OsHV-1 freedom.
Final Report • 2019-09-01 • 863.76 KB
2018-102-DLD.pdf

Summary

South Australia (SA) has a large edible oyster industry primarily growing Pacific oysters (Crassostrea gigas). The industry is regionally-based, an important employer and a substantial contributor to regional economies. Pacific oyster mortality syndrome (POMS) is a serious infectious disease of C. gigas caused by ostreid herpesvirus-1 microvariant (OsHV-1 microvariant). The first outbreak of OsHV-1 in Australia occurred in 2010, in the Georges River-Botany Bay and Port Hacking-Sydney Harbor estuaries in NSW. OsHV-1 was subsequently detected in the Hawkesbury River system (2013) and eastern Tasmania (2016). The production and economic impacts of these outbreaks have been substantial.
OsHV-1 infection was confirmed in the Port River, SA, in February 2018 in association with high mortality (50-90+%) of feral Pacific oysters. POMS was not identified in SA outside the control area, and surveillance has shown commercial growing areas in SA to be free of OsHV-1. Surveillance has shown that C. gigas in the Port River system has high prevalence of OsHV-1 infection.
Mussels (Mytilus spp.) share similar habitats to Pacific oysters and are common in the Port River estuary (1). Mussels are demonstrated as hosts for OsHV-1 in Ireland (2), and show some histopathological signs of disease but outbreaks in mussels are not described. We aimed to implement an OsHV-1 in situ hybridization (ISH) assay and assess OsHV-1 infection in PCR positive non-C. gigas hosts using ISH.
An in situ hybridization (ISH) test for OsHV-1 was implemented at Flinders University based on published primers for detection of the virus. ISH showed a strong signal in sections from infected C. gigas and none in uninfected C. gigas. Bivalves were collected in the Port River by SARDI, sampled and tested for OsHV-1 using PCR. Three PCR positive samples of Mytilus spp. were also positive by ISH, showing infection with OsHV-1. 
Confirmation that mussels are a host of OsHV-1 has a range of important management implications. Movement of mussels likely poses a risk for transmission of OsHV-1, so control activities designed to decrease host populations that target only Pacific oysters are unlikely to be successful; biofouling management should be general rather than targeting only Pacific oysters. Containment measures should target all bivalve species. Mussels for translocation to OsHV-1 free areas should be sourced from biosecure hatcheries and tested to provide evidence of OsHV-1 freedom.
Final Report • 2019-09-01 • 863.76 KB
2018-102-DLD.pdf

Summary

South Australia (SA) has a large edible oyster industry primarily growing Pacific oysters (Crassostrea gigas). The industry is regionally-based, an important employer and a substantial contributor to regional economies. Pacific oyster mortality syndrome (POMS) is a serious infectious disease of C. gigas caused by ostreid herpesvirus-1 microvariant (OsHV-1 microvariant). The first outbreak of OsHV-1 in Australia occurred in 2010, in the Georges River-Botany Bay and Port Hacking-Sydney Harbor estuaries in NSW. OsHV-1 was subsequently detected in the Hawkesbury River system (2013) and eastern Tasmania (2016). The production and economic impacts of these outbreaks have been substantial.
OsHV-1 infection was confirmed in the Port River, SA, in February 2018 in association with high mortality (50-90+%) of feral Pacific oysters. POMS was not identified in SA outside the control area, and surveillance has shown commercial growing areas in SA to be free of OsHV-1. Surveillance has shown that C. gigas in the Port River system has high prevalence of OsHV-1 infection.
Mussels (Mytilus spp.) share similar habitats to Pacific oysters and are common in the Port River estuary (1). Mussels are demonstrated as hosts for OsHV-1 in Ireland (2), and show some histopathological signs of disease but outbreaks in mussels are not described. We aimed to implement an OsHV-1 in situ hybridization (ISH) assay and assess OsHV-1 infection in PCR positive non-C. gigas hosts using ISH.
An in situ hybridization (ISH) test for OsHV-1 was implemented at Flinders University based on published primers for detection of the virus. ISH showed a strong signal in sections from infected C. gigas and none in uninfected C. gigas. Bivalves were collected in the Port River by SARDI, sampled and tested for OsHV-1 using PCR. Three PCR positive samples of Mytilus spp. were also positive by ISH, showing infection with OsHV-1. 
Confirmation that mussels are a host of OsHV-1 has a range of important management implications. Movement of mussels likely poses a risk for transmission of OsHV-1, so control activities designed to decrease host populations that target only Pacific oysters are unlikely to be successful; biofouling management should be general rather than targeting only Pacific oysters. Containment measures should target all bivalve species. Mussels for translocation to OsHV-1 free areas should be sourced from biosecure hatcheries and tested to provide evidence of OsHV-1 freedom.
Final Report • 2019-09-01 • 863.76 KB
2018-102-DLD.pdf

Summary

South Australia (SA) has a large edible oyster industry primarily growing Pacific oysters (Crassostrea gigas). The industry is regionally-based, an important employer and a substantial contributor to regional economies. Pacific oyster mortality syndrome (POMS) is a serious infectious disease of C. gigas caused by ostreid herpesvirus-1 microvariant (OsHV-1 microvariant). The first outbreak of OsHV-1 in Australia occurred in 2010, in the Georges River-Botany Bay and Port Hacking-Sydney Harbor estuaries in NSW. OsHV-1 was subsequently detected in the Hawkesbury River system (2013) and eastern Tasmania (2016). The production and economic impacts of these outbreaks have been substantial.
OsHV-1 infection was confirmed in the Port River, SA, in February 2018 in association with high mortality (50-90+%) of feral Pacific oysters. POMS was not identified in SA outside the control area, and surveillance has shown commercial growing areas in SA to be free of OsHV-1. Surveillance has shown that C. gigas in the Port River system has high prevalence of OsHV-1 infection.
Mussels (Mytilus spp.) share similar habitats to Pacific oysters and are common in the Port River estuary (1). Mussels are demonstrated as hosts for OsHV-1 in Ireland (2), and show some histopathological signs of disease but outbreaks in mussels are not described. We aimed to implement an OsHV-1 in situ hybridization (ISH) assay and assess OsHV-1 infection in PCR positive non-C. gigas hosts using ISH.
An in situ hybridization (ISH) test for OsHV-1 was implemented at Flinders University based on published primers for detection of the virus. ISH showed a strong signal in sections from infected C. gigas and none in uninfected C. gigas. Bivalves were collected in the Port River by SARDI, sampled and tested for OsHV-1 using PCR. Three PCR positive samples of Mytilus spp. were also positive by ISH, showing infection with OsHV-1. 
Confirmation that mussels are a host of OsHV-1 has a range of important management implications. Movement of mussels likely poses a risk for transmission of OsHV-1, so control activities designed to decrease host populations that target only Pacific oysters are unlikely to be successful; biofouling management should be general rather than targeting only Pacific oysters. Containment measures should target all bivalve species. Mussels for translocation to OsHV-1 free areas should be sourced from biosecure hatcheries and tested to provide evidence of OsHV-1 freedom.
Final Report • 2019-09-01 • 863.76 KB
2018-102-DLD.pdf

Summary

South Australia (SA) has a large edible oyster industry primarily growing Pacific oysters (Crassostrea gigas). The industry is regionally-based, an important employer and a substantial contributor to regional economies. Pacific oyster mortality syndrome (POMS) is a serious infectious disease of C. gigas caused by ostreid herpesvirus-1 microvariant (OsHV-1 microvariant). The first outbreak of OsHV-1 in Australia occurred in 2010, in the Georges River-Botany Bay and Port Hacking-Sydney Harbor estuaries in NSW. OsHV-1 was subsequently detected in the Hawkesbury River system (2013) and eastern Tasmania (2016). The production and economic impacts of these outbreaks have been substantial.
OsHV-1 infection was confirmed in the Port River, SA, in February 2018 in association with high mortality (50-90+%) of feral Pacific oysters. POMS was not identified in SA outside the control area, and surveillance has shown commercial growing areas in SA to be free of OsHV-1. Surveillance has shown that C. gigas in the Port River system has high prevalence of OsHV-1 infection.
Mussels (Mytilus spp.) share similar habitats to Pacific oysters and are common in the Port River estuary (1). Mussels are demonstrated as hosts for OsHV-1 in Ireland (2), and show some histopathological signs of disease but outbreaks in mussels are not described. We aimed to implement an OsHV-1 in situ hybridization (ISH) assay and assess OsHV-1 infection in PCR positive non-C. gigas hosts using ISH.
An in situ hybridization (ISH) test for OsHV-1 was implemented at Flinders University based on published primers for detection of the virus. ISH showed a strong signal in sections from infected C. gigas and none in uninfected C. gigas. Bivalves were collected in the Port River by SARDI, sampled and tested for OsHV-1 using PCR. Three PCR positive samples of Mytilus spp. were also positive by ISH, showing infection with OsHV-1. 
Confirmation that mussels are a host of OsHV-1 has a range of important management implications. Movement of mussels likely poses a risk for transmission of OsHV-1, so control activities designed to decrease host populations that target only Pacific oysters are unlikely to be successful; biofouling management should be general rather than targeting only Pacific oysters. Containment measures should target all bivalve species. Mussels for translocation to OsHV-1 free areas should be sourced from biosecure hatcheries and tested to provide evidence of OsHV-1 freedom.
Final Report • 2019-09-01 • 863.76 KB
2018-102-DLD.pdf

Summary

South Australia (SA) has a large edible oyster industry primarily growing Pacific oysters (Crassostrea gigas). The industry is regionally-based, an important employer and a substantial contributor to regional economies. Pacific oyster mortality syndrome (POMS) is a serious infectious disease of C. gigas caused by ostreid herpesvirus-1 microvariant (OsHV-1 microvariant). The first outbreak of OsHV-1 in Australia occurred in 2010, in the Georges River-Botany Bay and Port Hacking-Sydney Harbor estuaries in NSW. OsHV-1 was subsequently detected in the Hawkesbury River system (2013) and eastern Tasmania (2016). The production and economic impacts of these outbreaks have been substantial.
OsHV-1 infection was confirmed in the Port River, SA, in February 2018 in association with high mortality (50-90+%) of feral Pacific oysters. POMS was not identified in SA outside the control area, and surveillance has shown commercial growing areas in SA to be free of OsHV-1. Surveillance has shown that C. gigas in the Port River system has high prevalence of OsHV-1 infection.
Mussels (Mytilus spp.) share similar habitats to Pacific oysters and are common in the Port River estuary (1). Mussels are demonstrated as hosts for OsHV-1 in Ireland (2), and show some histopathological signs of disease but outbreaks in mussels are not described. We aimed to implement an OsHV-1 in situ hybridization (ISH) assay and assess OsHV-1 infection in PCR positive non-C. gigas hosts using ISH.
An in situ hybridization (ISH) test for OsHV-1 was implemented at Flinders University based on published primers for detection of the virus. ISH showed a strong signal in sections from infected C. gigas and none in uninfected C. gigas. Bivalves were collected in the Port River by SARDI, sampled and tested for OsHV-1 using PCR. Three PCR positive samples of Mytilus spp. were also positive by ISH, showing infection with OsHV-1. 
Confirmation that mussels are a host of OsHV-1 has a range of important management implications. Movement of mussels likely poses a risk for transmission of OsHV-1, so control activities designed to decrease host populations that target only Pacific oysters are unlikely to be successful; biofouling management should be general rather than targeting only Pacific oysters. Containment measures should target all bivalve species. Mussels for translocation to OsHV-1 free areas should be sourced from biosecure hatcheries and tested to provide evidence of OsHV-1 freedom.
Final Report • 2019-09-01 • 863.76 KB
2018-102-DLD.pdf

Summary

South Australia (SA) has a large edible oyster industry primarily growing Pacific oysters (Crassostrea gigas). The industry is regionally-based, an important employer and a substantial contributor to regional economies. Pacific oyster mortality syndrome (POMS) is a serious infectious disease of C. gigas caused by ostreid herpesvirus-1 microvariant (OsHV-1 microvariant). The first outbreak of OsHV-1 in Australia occurred in 2010, in the Georges River-Botany Bay and Port Hacking-Sydney Harbor estuaries in NSW. OsHV-1 was subsequently detected in the Hawkesbury River system (2013) and eastern Tasmania (2016). The production and economic impacts of these outbreaks have been substantial.
OsHV-1 infection was confirmed in the Port River, SA, in February 2018 in association with high mortality (50-90+%) of feral Pacific oysters. POMS was not identified in SA outside the control area, and surveillance has shown commercial growing areas in SA to be free of OsHV-1. Surveillance has shown that C. gigas in the Port River system has high prevalence of OsHV-1 infection.
Mussels (Mytilus spp.) share similar habitats to Pacific oysters and are common in the Port River estuary (1). Mussels are demonstrated as hosts for OsHV-1 in Ireland (2), and show some histopathological signs of disease but outbreaks in mussels are not described. We aimed to implement an OsHV-1 in situ hybridization (ISH) assay and assess OsHV-1 infection in PCR positive non-C. gigas hosts using ISH.
An in situ hybridization (ISH) test for OsHV-1 was implemented at Flinders University based on published primers for detection of the virus. ISH showed a strong signal in sections from infected C. gigas and none in uninfected C. gigas. Bivalves were collected in the Port River by SARDI, sampled and tested for OsHV-1 using PCR. Three PCR positive samples of Mytilus spp. were also positive by ISH, showing infection with OsHV-1. 
Confirmation that mussels are a host of OsHV-1 has a range of important management implications. Movement of mussels likely poses a risk for transmission of OsHV-1, so control activities designed to decrease host populations that target only Pacific oysters are unlikely to be successful; biofouling management should be general rather than targeting only Pacific oysters. Containment measures should target all bivalve species. Mussels for translocation to OsHV-1 free areas should be sourced from biosecure hatcheries and tested to provide evidence of OsHV-1 freedom.
Final Report • 2019-09-01 • 863.76 KB
2018-102-DLD.pdf

Summary

South Australia (SA) has a large edible oyster industry primarily growing Pacific oysters (Crassostrea gigas). The industry is regionally-based, an important employer and a substantial contributor to regional economies. Pacific oyster mortality syndrome (POMS) is a serious infectious disease of C. gigas caused by ostreid herpesvirus-1 microvariant (OsHV-1 microvariant). The first outbreak of OsHV-1 in Australia occurred in 2010, in the Georges River-Botany Bay and Port Hacking-Sydney Harbor estuaries in NSW. OsHV-1 was subsequently detected in the Hawkesbury River system (2013) and eastern Tasmania (2016). The production and economic impacts of these outbreaks have been substantial.
OsHV-1 infection was confirmed in the Port River, SA, in February 2018 in association with high mortality (50-90+%) of feral Pacific oysters. POMS was not identified in SA outside the control area, and surveillance has shown commercial growing areas in SA to be free of OsHV-1. Surveillance has shown that C. gigas in the Port River system has high prevalence of OsHV-1 infection.
Mussels (Mytilus spp.) share similar habitats to Pacific oysters and are common in the Port River estuary (1). Mussels are demonstrated as hosts for OsHV-1 in Ireland (2), and show some histopathological signs of disease but outbreaks in mussels are not described. We aimed to implement an OsHV-1 in situ hybridization (ISH) assay and assess OsHV-1 infection in PCR positive non-C. gigas hosts using ISH.
An in situ hybridization (ISH) test for OsHV-1 was implemented at Flinders University based on published primers for detection of the virus. ISH showed a strong signal in sections from infected C. gigas and none in uninfected C. gigas. Bivalves were collected in the Port River by SARDI, sampled and tested for OsHV-1 using PCR. Three PCR positive samples of Mytilus spp. were also positive by ISH, showing infection with OsHV-1. 
Confirmation that mussels are a host of OsHV-1 has a range of important management implications. Movement of mussels likely poses a risk for transmission of OsHV-1, so control activities designed to decrease host populations that target only Pacific oysters are unlikely to be successful; biofouling management should be general rather than targeting only Pacific oysters. Containment measures should target all bivalve species. Mussels for translocation to OsHV-1 free areas should be sourced from biosecure hatcheries and tested to provide evidence of OsHV-1 freedom.
Final Report • 2019-09-01 • 863.76 KB
2018-102-DLD.pdf

Summary

South Australia (SA) has a large edible oyster industry primarily growing Pacific oysters (Crassostrea gigas). The industry is regionally-based, an important employer and a substantial contributor to regional economies. Pacific oyster mortality syndrome (POMS) is a serious infectious disease of C. gigas caused by ostreid herpesvirus-1 microvariant (OsHV-1 microvariant). The first outbreak of OsHV-1 in Australia occurred in 2010, in the Georges River-Botany Bay and Port Hacking-Sydney Harbor estuaries in NSW. OsHV-1 was subsequently detected in the Hawkesbury River system (2013) and eastern Tasmania (2016). The production and economic impacts of these outbreaks have been substantial.
OsHV-1 infection was confirmed in the Port River, SA, in February 2018 in association with high mortality (50-90+%) of feral Pacific oysters. POMS was not identified in SA outside the control area, and surveillance has shown commercial growing areas in SA to be free of OsHV-1. Surveillance has shown that C. gigas in the Port River system has high prevalence of OsHV-1 infection.
Mussels (Mytilus spp.) share similar habitats to Pacific oysters and are common in the Port River estuary (1). Mussels are demonstrated as hosts for OsHV-1 in Ireland (2), and show some histopathological signs of disease but outbreaks in mussels are not described. We aimed to implement an OsHV-1 in situ hybridization (ISH) assay and assess OsHV-1 infection in PCR positive non-C. gigas hosts using ISH.
An in situ hybridization (ISH) test for OsHV-1 was implemented at Flinders University based on published primers for detection of the virus. ISH showed a strong signal in sections from infected C. gigas and none in uninfected C. gigas. Bivalves were collected in the Port River by SARDI, sampled and tested for OsHV-1 using PCR. Three PCR positive samples of Mytilus spp. were also positive by ISH, showing infection with OsHV-1. 
Confirmation that mussels are a host of OsHV-1 has a range of important management implications. Movement of mussels likely poses a risk for transmission of OsHV-1, so control activities designed to decrease host populations that target only Pacific oysters are unlikely to be successful; biofouling management should be general rather than targeting only Pacific oysters. Containment measures should target all bivalve species. Mussels for translocation to OsHV-1 free areas should be sourced from biosecure hatcheries and tested to provide evidence of OsHV-1 freedom.
Final Report • 2019-09-01 • 863.76 KB
2018-102-DLD.pdf

Summary

South Australia (SA) has a large edible oyster industry primarily growing Pacific oysters (Crassostrea gigas). The industry is regionally-based, an important employer and a substantial contributor to regional economies. Pacific oyster mortality syndrome (POMS) is a serious infectious disease of C. gigas caused by ostreid herpesvirus-1 microvariant (OsHV-1 microvariant). The first outbreak of OsHV-1 in Australia occurred in 2010, in the Georges River-Botany Bay and Port Hacking-Sydney Harbor estuaries in NSW. OsHV-1 was subsequently detected in the Hawkesbury River system (2013) and eastern Tasmania (2016). The production and economic impacts of these outbreaks have been substantial.
OsHV-1 infection was confirmed in the Port River, SA, in February 2018 in association with high mortality (50-90+%) of feral Pacific oysters. POMS was not identified in SA outside the control area, and surveillance has shown commercial growing areas in SA to be free of OsHV-1. Surveillance has shown that C. gigas in the Port River system has high prevalence of OsHV-1 infection.
Mussels (Mytilus spp.) share similar habitats to Pacific oysters and are common in the Port River estuary (1). Mussels are demonstrated as hosts for OsHV-1 in Ireland (2), and show some histopathological signs of disease but outbreaks in mussels are not described. We aimed to implement an OsHV-1 in situ hybridization (ISH) assay and assess OsHV-1 infection in PCR positive non-C. gigas hosts using ISH.
An in situ hybridization (ISH) test for OsHV-1 was implemented at Flinders University based on published primers for detection of the virus. ISH showed a strong signal in sections from infected C. gigas and none in uninfected C. gigas. Bivalves were collected in the Port River by SARDI, sampled and tested for OsHV-1 using PCR. Three PCR positive samples of Mytilus spp. were also positive by ISH, showing infection with OsHV-1. 
Confirmation that mussels are a host of OsHV-1 has a range of important management implications. Movement of mussels likely poses a risk for transmission of OsHV-1, so control activities designed to decrease host populations that target only Pacific oysters are unlikely to be successful; biofouling management should be general rather than targeting only Pacific oysters. Containment measures should target all bivalve species. Mussels for translocation to OsHV-1 free areas should be sourced from biosecure hatcheries and tested to provide evidence of OsHV-1 freedom.
Final Report • 2019-09-01 • 863.76 KB
2018-102-DLD.pdf

Summary

South Australia (SA) has a large edible oyster industry primarily growing Pacific oysters (Crassostrea gigas). The industry is regionally-based, an important employer and a substantial contributor to regional economies. Pacific oyster mortality syndrome (POMS) is a serious infectious disease of C. gigas caused by ostreid herpesvirus-1 microvariant (OsHV-1 microvariant). The first outbreak of OsHV-1 in Australia occurred in 2010, in the Georges River-Botany Bay and Port Hacking-Sydney Harbor estuaries in NSW. OsHV-1 was subsequently detected in the Hawkesbury River system (2013) and eastern Tasmania (2016). The production and economic impacts of these outbreaks have been substantial.
OsHV-1 infection was confirmed in the Port River, SA, in February 2018 in association with high mortality (50-90+%) of feral Pacific oysters. POMS was not identified in SA outside the control area, and surveillance has shown commercial growing areas in SA to be free of OsHV-1. Surveillance has shown that C. gigas in the Port River system has high prevalence of OsHV-1 infection.
Mussels (Mytilus spp.) share similar habitats to Pacific oysters and are common in the Port River estuary (1). Mussels are demonstrated as hosts for OsHV-1 in Ireland (2), and show some histopathological signs of disease but outbreaks in mussels are not described. We aimed to implement an OsHV-1 in situ hybridization (ISH) assay and assess OsHV-1 infection in PCR positive non-C. gigas hosts using ISH.
An in situ hybridization (ISH) test for OsHV-1 was implemented at Flinders University based on published primers for detection of the virus. ISH showed a strong signal in sections from infected C. gigas and none in uninfected C. gigas. Bivalves were collected in the Port River by SARDI, sampled and tested for OsHV-1 using PCR. Three PCR positive samples of Mytilus spp. were also positive by ISH, showing infection with OsHV-1. 
Confirmation that mussels are a host of OsHV-1 has a range of important management implications. Movement of mussels likely poses a risk for transmission of OsHV-1, so control activities designed to decrease host populations that target only Pacific oysters are unlikely to be successful; biofouling management should be general rather than targeting only Pacific oysters. Containment measures should target all bivalve species. Mussels for translocation to OsHV-1 free areas should be sourced from biosecure hatcheries and tested to provide evidence of OsHV-1 freedom.

A trivalent vaccine for sustainable Yellowtail Kingfish growout

Project number: 2018-101
Project Status:
Current
Budget expenditure: $809,040.00
Principal Investigator: Andrew C. Barnes
Organisation: University of Queensland (UQ)
Project start/end date: 29 Nov 2018 - 14 Dec 2021
Contact:
FRDC
TAGS
Vaccine
Sustainability
Disease
Aquaculture
Animal Health
SPECIES
Yellowtail Kingfish

Need

Optimised vaccines deliver improved animal health and avoid use of antimicrobials in the production of fish (Sommerset et al., Expert Review Vaccines 2005, 4, 89-101.). Australian YTK Photobacteriosis outbreaks have been reported in association with Photobacterium damselae subsp damselae (Pdd), Photobacterium damselae subspecies piscicida(Pdp) and various Vibrio harveyi(Vh) isolates (Matt Landos, Nicky Buller, unpublished). To support stable production of high quality finfish highly efficacious controls of microbial diseases are critical. Successful examples are currently employed in salmon (Yersinia ruckeri, Vibrio anguillarum) and barramundi (Streptococcus iniae) whereby disease outbreaks drove the need for vaccine development. These conditions are now both well controlled in vaccinated populations and have dramatically reduced reliance on administration of antibiotics.
Currently, crude autogenous vaccines are in use with YTK for control of only Pdd in South Australia and Western Australia with some reported success. Recently Pdp has emerged as a significant pathogen in South Australian outbreaks. Reliable vaccination against Pdp has proven difficult in international experience using traditional autogenous and generic killed vaccine methods, hence increasingly research is exploring DNA vaccination methods to improve protection against this pathogen (Kato et al., Vaccine 2015, 33 (8), 1040-1045). It is also probable that antigenic diversity had been underestimated, given high diversity that recent sequencing technology has revealed in our preliminary research . Preparedness for the emergence of highly pathogenic Pdp strains is prudent to allow rapid controls to be developed, without incurring business-ending losses within the growing aquaculture sector, or having high levels of reliance on oral antibiotics. Genetic sequence data on isolates generated through this project can be used for epidemiological modelling, new rapid diagnostic typing tools and immediate response if disease emergence occurs in other finfish industries. Pdp is acknowledged as a serious pathogen internationally in species including yellowtail, cobia, flounder, seabream, pompano and sea bass. (Andreoni et al.J Immunol Res. 2014).

Objectives

1. Generate comprehensive genome database of Australian isolates of Pdd, Pdp and Vh associated with Photobacteriosis outbreaks in YTK
2. Generate and supply diagnostic laboratory reagents and methods for typing Photobacterium and Vibrio isolates associated with YTK outbreaks.
3. Deliver optimised monovalent and multivalent (Pdd, Pdp, Vh) killed vaccines, with production outline, appropriate adjuvant type, dose, safety and efficacy profile in YTK, ready for manufacture.

Optimisation of treatment of Cryptocaryon irritans in Barramundi aquaculture

Project number: 2018-100
Project Status:
Current
Budget expenditure: $415,057.00
Principal Investigator: Marty R. Deveney
Organisation: University of Adelaide
Project start/end date: 30 Sep 2021 - 29 Aug 2024
Contact:
FRDC
SPECIES
Cobia
Yellowspotted Rockcod
Goldspotted Rockcod
Barramundi

Need

Significant losses of farmed barramundi have occurred in coastal marine aquaculture sites utilising marine water sources due to Cryptocaryon irritans infestations. The disease costs have had substantial impacts on commercial aquaculture operations. Cryptocaryon irritans has a direct life cycle with an adult in the skin or gills of the fish and asexual reproduction in the environment away from the fish. The life cycle of C. irritans is temperature and host dependant and treatment must be timed with critical phases of the life history to maximise efficacy. This project will establish this timing to provide guidelines for optimised treatment.

The existing treatments have substantial costs ($3K+/treatment) and a sub-optimal profile for environmental release with high toxicity and environmental persistence. This project will optimise doses of existing treatments, and identify and assess new treatments. Products identified as effective and safe will have optimised guidelines developed for delivery to ensure the best outcome for farms and the environment. Such improvements will support further expansion of the finfish aquaculture industry in Northern Australia.

Objectives

1. Describe the life cycle of Australian C. irritans isolates from Barramundi aquaculture, and describe relevant treatment intervals for different temperatures for C. irritans to maximise benefit from each treatment
2. Screen candidate treatments to identify new products for further assessment
3. Establish efficacy and safety of optimised doses for current treatments and selected candidate treatments in laboratory and small-scale field trials
4. Optimise delivery under field conditions for delivery of new treatments
Industry
PROJECT NUMBER • 2018-099
PROJECT STATUS:
COMPLETED

Research to provide data to support application for a minor use permits for chemicals including trichlorfon, chlorine, copper sulfate, hydrogen peroxide, benzylkonium chloride for control of disease including White Spot Syndrome Virus (WSSV) in Australian prawn farms.

The project involved generation of chemical data packages through review and aggregation of public domain documents for each chemical (trichlorfon, hydrogen peroxide, sodium and calcium hypochlorite, copper sulphate and benzalkonium chloride). This facilitated identification of data gaps requiring...
ORGANISATION:
Future Fisheries Veterinary Service Pty Ltd (FFVS)
TAGS
Disease
Contamination
Biosecurity
Audit
Aquaculture
SPECIES
Black Tiger Prawn
Redspot King Prawn
King Prawns
Banana Prawns
Tiger Prawns

Vaccination for emergency and long-term control of nodavirus in Australian marine aquaculture

Project number: 2018-098
Project Status:
Completed
Budget expenditure: $567,695.00
Principal Investigator: Andrew C. Barnes
Organisation: University of Queensland (UQ)
Project start/end date: 28 Mar 2019 - 30 Jan 2023
Contact:
FRDC
TAGS
Rac Qld
SPECIES
Cobia

Need

The grouper industry is new but growing in Australia with recent new private sector investment in the hatchery in Cairns. However, all current grow out is affected by nodavirus outbreaks which have been both acute and severe. Without a solution the industry will not be able to continue. Fortunately, nodavirus is preventable by vaccination and there is local capacity to produce. Whilst there is currently insufficient demand to allow a fully privately financed vaccine initiative, with an emergency vaccine put in place and a registration data pack established, the industry is predicted to grow to a point where vaccine production by the private sector will become self-sustaining. This project will deliver an emergency vaccine to assist farmers through 2018/2019 grow out. It will build capability in vaccination of grouper in the industry. Most importantly it will provide the efficacy data, optimisation and formulation data with documentation appropriate for future licensing of a vaccine for use in Australia.

Objectives

1. An emergency vaccine to prevent nodavirus in the Queensland grouper cohort for stocking in spring/summer 2018
2. A data pack suitable for registration of an optimally formulated nodavirus vaccine for Australia

Final report

ISBN: 978-1-74272-399-0
Authors: Rosemary Thwaite Minami Kawasaki Angus Li Kelly Condon Richard Knuckey Bradley Cherrie Mark White Matt Landos and Andrew C Barnes
Final Report • 2023-02-01 • 18.85 MB
2018-098-DLD.pdf

Summary

Rocky Point Aquaculture in southeast Queensland experienced a disease outbreak in cage-reared giant grouper (Epinephelus lanceolatus) in late summer and autumn 2018 resulting is severe losses caused by a Betanodavirus. Following a request from the farm owner, Serena Zipf in July 2018, Dr Andrew Barnes from The University of Queensland, designed and built a recombinant protein subunit vaccine against the Betanodavirus and supplied the construct and production outline to Tréidlia BioVet Pty Ltd (Sydney) for manufacture in September 2018. Under direction from Dr Matt Landos and Dr James Fensham (Future Fisheries Veterinary Services) and with permission from Dr Richard Knuckey (General Manager, The Company One), juvenile grouper were vaccinated under a research permit from the Australian Pesticides and Veterinary Medicines Authority (APVMA) at The Company One (TCO) Cairns hatchery in October 2018, and shipped for grow-out at Rocky Point Aquaculture in December 2018, less than 6 months after concept discussion. Vaccination coincided with a reduction in the severity of outbreaks and from mid-2019 to 2022 the farm was free from nodavirus outbreaks. In summer 2022, a persistent low-level  outbreak was recorded at the farm in vaccinated fish, although again well below the mortality levels of the 2018 outbreak. In controlled laboratory challenge studies by Dr Kelly Condon at James Cook University, the vaccine has been shown to be >90% effective. Vaccinated juveniles have also been purchased by Noosa Ecomarine and the vaccine has also coincided with substantially reduced mortalities by nodavirus in their recirculating aquaculture facility.
Final Report • 2023-02-01 • 18.85 MB
2018-098-DLD.pdf

Summary

Rocky Point Aquaculture in southeast Queensland experienced a disease outbreak in cage-reared giant grouper (Epinephelus lanceolatus) in late summer and autumn 2018 resulting is severe losses caused by a Betanodavirus. Following a request from the farm owner, Serena Zipf in July 2018, Dr Andrew Barnes from The University of Queensland, designed and built a recombinant protein subunit vaccine against the Betanodavirus and supplied the construct and production outline to Tréidlia BioVet Pty Ltd (Sydney) for manufacture in September 2018. Under direction from Dr Matt Landos and Dr James Fensham (Future Fisheries Veterinary Services) and with permission from Dr Richard Knuckey (General Manager, The Company One), juvenile grouper were vaccinated under a research permit from the Australian Pesticides and Veterinary Medicines Authority (APVMA) at The Company One (TCO) Cairns hatchery in October 2018, and shipped for grow-out at Rocky Point Aquaculture in December 2018, less than 6 months after concept discussion. Vaccination coincided with a reduction in the severity of outbreaks and from mid-2019 to 2022 the farm was free from nodavirus outbreaks. In summer 2022, a persistent low-level  outbreak was recorded at the farm in vaccinated fish, although again well below the mortality levels of the 2018 outbreak. In controlled laboratory challenge studies by Dr Kelly Condon at James Cook University, the vaccine has been shown to be >90% effective. Vaccinated juveniles have also been purchased by Noosa Ecomarine and the vaccine has also coincided with substantially reduced mortalities by nodavirus in their recirculating aquaculture facility.
Final Report • 2023-02-01 • 18.85 MB
2018-098-DLD.pdf

Summary

Rocky Point Aquaculture in southeast Queensland experienced a disease outbreak in cage-reared giant grouper (Epinephelus lanceolatus) in late summer and autumn 2018 resulting is severe losses caused by a Betanodavirus. Following a request from the farm owner, Serena Zipf in July 2018, Dr Andrew Barnes from The University of Queensland, designed and built a recombinant protein subunit vaccine against the Betanodavirus and supplied the construct and production outline to Tréidlia BioVet Pty Ltd (Sydney) for manufacture in September 2018. Under direction from Dr Matt Landos and Dr James Fensham (Future Fisheries Veterinary Services) and with permission from Dr Richard Knuckey (General Manager, The Company One), juvenile grouper were vaccinated under a research permit from the Australian Pesticides and Veterinary Medicines Authority (APVMA) at The Company One (TCO) Cairns hatchery in October 2018, and shipped for grow-out at Rocky Point Aquaculture in December 2018, less than 6 months after concept discussion. Vaccination coincided with a reduction in the severity of outbreaks and from mid-2019 to 2022 the farm was free from nodavirus outbreaks. In summer 2022, a persistent low-level  outbreak was recorded at the farm in vaccinated fish, although again well below the mortality levels of the 2018 outbreak. In controlled laboratory challenge studies by Dr Kelly Condon at James Cook University, the vaccine has been shown to be >90% effective. Vaccinated juveniles have also been purchased by Noosa Ecomarine and the vaccine has also coincided with substantially reduced mortalities by nodavirus in their recirculating aquaculture facility.
Final Report • 2023-02-01 • 18.85 MB
2018-098-DLD.pdf

Summary

Rocky Point Aquaculture in southeast Queensland experienced a disease outbreak in cage-reared giant grouper (Epinephelus lanceolatus) in late summer and autumn 2018 resulting is severe losses caused by a Betanodavirus. Following a request from the farm owner, Serena Zipf in July 2018, Dr Andrew Barnes from The University of Queensland, designed and built a recombinant protein subunit vaccine against the Betanodavirus and supplied the construct and production outline to Tréidlia BioVet Pty Ltd (Sydney) for manufacture in September 2018. Under direction from Dr Matt Landos and Dr James Fensham (Future Fisheries Veterinary Services) and with permission from Dr Richard Knuckey (General Manager, The Company One), juvenile grouper were vaccinated under a research permit from the Australian Pesticides and Veterinary Medicines Authority (APVMA) at The Company One (TCO) Cairns hatchery in October 2018, and shipped for grow-out at Rocky Point Aquaculture in December 2018, less than 6 months after concept discussion. Vaccination coincided with a reduction in the severity of outbreaks and from mid-2019 to 2022 the farm was free from nodavirus outbreaks. In summer 2022, a persistent low-level  outbreak was recorded at the farm in vaccinated fish, although again well below the mortality levels of the 2018 outbreak. In controlled laboratory challenge studies by Dr Kelly Condon at James Cook University, the vaccine has been shown to be >90% effective. Vaccinated juveniles have also been purchased by Noosa Ecomarine and the vaccine has also coincided with substantially reduced mortalities by nodavirus in their recirculating aquaculture facility.
Final Report • 2023-02-01 • 18.85 MB
2018-098-DLD.pdf

Summary

Rocky Point Aquaculture in southeast Queensland experienced a disease outbreak in cage-reared giant grouper (Epinephelus lanceolatus) in late summer and autumn 2018 resulting is severe losses caused by a Betanodavirus. Following a request from the farm owner, Serena Zipf in July 2018, Dr Andrew Barnes from The University of Queensland, designed and built a recombinant protein subunit vaccine against the Betanodavirus and supplied the construct and production outline to Tréidlia BioVet Pty Ltd (Sydney) for manufacture in September 2018. Under direction from Dr Matt Landos and Dr James Fensham (Future Fisheries Veterinary Services) and with permission from Dr Richard Knuckey (General Manager, The Company One), juvenile grouper were vaccinated under a research permit from the Australian Pesticides and Veterinary Medicines Authority (APVMA) at The Company One (TCO) Cairns hatchery in October 2018, and shipped for grow-out at Rocky Point Aquaculture in December 2018, less than 6 months after concept discussion. Vaccination coincided with a reduction in the severity of outbreaks and from mid-2019 to 2022 the farm was free from nodavirus outbreaks. In summer 2022, a persistent low-level  outbreak was recorded at the farm in vaccinated fish, although again well below the mortality levels of the 2018 outbreak. In controlled laboratory challenge studies by Dr Kelly Condon at James Cook University, the vaccine has been shown to be >90% effective. Vaccinated juveniles have also been purchased by Noosa Ecomarine and the vaccine has also coincided with substantially reduced mortalities by nodavirus in their recirculating aquaculture facility.
Final Report • 2023-02-01 • 18.85 MB
2018-098-DLD.pdf

Summary

Rocky Point Aquaculture in southeast Queensland experienced a disease outbreak in cage-reared giant grouper (Epinephelus lanceolatus) in late summer and autumn 2018 resulting is severe losses caused by a Betanodavirus. Following a request from the farm owner, Serena Zipf in July 2018, Dr Andrew Barnes from The University of Queensland, designed and built a recombinant protein subunit vaccine against the Betanodavirus and supplied the construct and production outline to Tréidlia BioVet Pty Ltd (Sydney) for manufacture in September 2018. Under direction from Dr Matt Landos and Dr James Fensham (Future Fisheries Veterinary Services) and with permission from Dr Richard Knuckey (General Manager, The Company One), juvenile grouper were vaccinated under a research permit from the Australian Pesticides and Veterinary Medicines Authority (APVMA) at The Company One (TCO) Cairns hatchery in October 2018, and shipped for grow-out at Rocky Point Aquaculture in December 2018, less than 6 months after concept discussion. Vaccination coincided with a reduction in the severity of outbreaks and from mid-2019 to 2022 the farm was free from nodavirus outbreaks. In summer 2022, a persistent low-level  outbreak was recorded at the farm in vaccinated fish, although again well below the mortality levels of the 2018 outbreak. In controlled laboratory challenge studies by Dr Kelly Condon at James Cook University, the vaccine has been shown to be >90% effective. Vaccinated juveniles have also been purchased by Noosa Ecomarine and the vaccine has also coincided with substantially reduced mortalities by nodavirus in their recirculating aquaculture facility.
Final Report • 2023-02-01 • 18.85 MB
2018-098-DLD.pdf

Summary

Rocky Point Aquaculture in southeast Queensland experienced a disease outbreak in cage-reared giant grouper (Epinephelus lanceolatus) in late summer and autumn 2018 resulting is severe losses caused by a Betanodavirus. Following a request from the farm owner, Serena Zipf in July 2018, Dr Andrew Barnes from The University of Queensland, designed and built a recombinant protein subunit vaccine against the Betanodavirus and supplied the construct and production outline to Tréidlia BioVet Pty Ltd (Sydney) for manufacture in September 2018. Under direction from Dr Matt Landos and Dr James Fensham (Future Fisheries Veterinary Services) and with permission from Dr Richard Knuckey (General Manager, The Company One), juvenile grouper were vaccinated under a research permit from the Australian Pesticides and Veterinary Medicines Authority (APVMA) at The Company One (TCO) Cairns hatchery in October 2018, and shipped for grow-out at Rocky Point Aquaculture in December 2018, less than 6 months after concept discussion. Vaccination coincided with a reduction in the severity of outbreaks and from mid-2019 to 2022 the farm was free from nodavirus outbreaks. In summer 2022, a persistent low-level  outbreak was recorded at the farm in vaccinated fish, although again well below the mortality levels of the 2018 outbreak. In controlled laboratory challenge studies by Dr Kelly Condon at James Cook University, the vaccine has been shown to be >90% effective. Vaccinated juveniles have also been purchased by Noosa Ecomarine and the vaccine has also coincided with substantially reduced mortalities by nodavirus in their recirculating aquaculture facility.
Final Report • 2023-02-01 • 18.85 MB
2018-098-DLD.pdf

Summary

Rocky Point Aquaculture in southeast Queensland experienced a disease outbreak in cage-reared giant grouper (Epinephelus lanceolatus) in late summer and autumn 2018 resulting is severe losses caused by a Betanodavirus. Following a request from the farm owner, Serena Zipf in July 2018, Dr Andrew Barnes from The University of Queensland, designed and built a recombinant protein subunit vaccine against the Betanodavirus and supplied the construct and production outline to Tréidlia BioVet Pty Ltd (Sydney) for manufacture in September 2018. Under direction from Dr Matt Landos and Dr James Fensham (Future Fisheries Veterinary Services) and with permission from Dr Richard Knuckey (General Manager, The Company One), juvenile grouper were vaccinated under a research permit from the Australian Pesticides and Veterinary Medicines Authority (APVMA) at The Company One (TCO) Cairns hatchery in October 2018, and shipped for grow-out at Rocky Point Aquaculture in December 2018, less than 6 months after concept discussion. Vaccination coincided with a reduction in the severity of outbreaks and from mid-2019 to 2022 the farm was free from nodavirus outbreaks. In summer 2022, a persistent low-level  outbreak was recorded at the farm in vaccinated fish, although again well below the mortality levels of the 2018 outbreak. In controlled laboratory challenge studies by Dr Kelly Condon at James Cook University, the vaccine has been shown to be >90% effective. Vaccinated juveniles have also been purchased by Noosa Ecomarine and the vaccine has also coincided with substantially reduced mortalities by nodavirus in their recirculating aquaculture facility.
Final Report • 2023-02-01 • 18.85 MB
2018-098-DLD.pdf

Summary

Rocky Point Aquaculture in southeast Queensland experienced a disease outbreak in cage-reared giant grouper (Epinephelus lanceolatus) in late summer and autumn 2018 resulting is severe losses caused by a Betanodavirus. Following a request from the farm owner, Serena Zipf in July 2018, Dr Andrew Barnes from The University of Queensland, designed and built a recombinant protein subunit vaccine against the Betanodavirus and supplied the construct and production outline to Tréidlia BioVet Pty Ltd (Sydney) for manufacture in September 2018. Under direction from Dr Matt Landos and Dr James Fensham (Future Fisheries Veterinary Services) and with permission from Dr Richard Knuckey (General Manager, The Company One), juvenile grouper were vaccinated under a research permit from the Australian Pesticides and Veterinary Medicines Authority (APVMA) at The Company One (TCO) Cairns hatchery in October 2018, and shipped for grow-out at Rocky Point Aquaculture in December 2018, less than 6 months after concept discussion. Vaccination coincided with a reduction in the severity of outbreaks and from mid-2019 to 2022 the farm was free from nodavirus outbreaks. In summer 2022, a persistent low-level  outbreak was recorded at the farm in vaccinated fish, although again well below the mortality levels of the 2018 outbreak. In controlled laboratory challenge studies by Dr Kelly Condon at James Cook University, the vaccine has been shown to be >90% effective. Vaccinated juveniles have also been purchased by Noosa Ecomarine and the vaccine has also coincided with substantially reduced mortalities by nodavirus in their recirculating aquaculture facility.
Final Report • 2023-02-01 • 18.85 MB
2018-098-DLD.pdf

Summary

Rocky Point Aquaculture in southeast Queensland experienced a disease outbreak in cage-reared giant grouper (Epinephelus lanceolatus) in late summer and autumn 2018 resulting is severe losses caused by a Betanodavirus. Following a request from the farm owner, Serena Zipf in July 2018, Dr Andrew Barnes from The University of Queensland, designed and built a recombinant protein subunit vaccine against the Betanodavirus and supplied the construct and production outline to Tréidlia BioVet Pty Ltd (Sydney) for manufacture in September 2018. Under direction from Dr Matt Landos and Dr James Fensham (Future Fisheries Veterinary Services) and with permission from Dr Richard Knuckey (General Manager, The Company One), juvenile grouper were vaccinated under a research permit from the Australian Pesticides and Veterinary Medicines Authority (APVMA) at The Company One (TCO) Cairns hatchery in October 2018, and shipped for grow-out at Rocky Point Aquaculture in December 2018, less than 6 months after concept discussion. Vaccination coincided with a reduction in the severity of outbreaks and from mid-2019 to 2022 the farm was free from nodavirus outbreaks. In summer 2022, a persistent low-level  outbreak was recorded at the farm in vaccinated fish, although again well below the mortality levels of the 2018 outbreak. In controlled laboratory challenge studies by Dr Kelly Condon at James Cook University, the vaccine has been shown to be >90% effective. Vaccinated juveniles have also been purchased by Noosa Ecomarine and the vaccine has also coincided with substantially reduced mortalities by nodavirus in their recirculating aquaculture facility.
Final Report • 2023-02-01 • 18.85 MB
2018-098-DLD.pdf

Summary

Rocky Point Aquaculture in southeast Queensland experienced a disease outbreak in cage-reared giant grouper (Epinephelus lanceolatus) in late summer and autumn 2018 resulting is severe losses caused by a Betanodavirus. Following a request from the farm owner, Serena Zipf in July 2018, Dr Andrew Barnes from The University of Queensland, designed and built a recombinant protein subunit vaccine against the Betanodavirus and supplied the construct and production outline to Tréidlia BioVet Pty Ltd (Sydney) for manufacture in September 2018. Under direction from Dr Matt Landos and Dr James Fensham (Future Fisheries Veterinary Services) and with permission from Dr Richard Knuckey (General Manager, The Company One), juvenile grouper were vaccinated under a research permit from the Australian Pesticides and Veterinary Medicines Authority (APVMA) at The Company One (TCO) Cairns hatchery in October 2018, and shipped for grow-out at Rocky Point Aquaculture in December 2018, less than 6 months after concept discussion. Vaccination coincided with a reduction in the severity of outbreaks and from mid-2019 to 2022 the farm was free from nodavirus outbreaks. In summer 2022, a persistent low-level  outbreak was recorded at the farm in vaccinated fish, although again well below the mortality levels of the 2018 outbreak. In controlled laboratory challenge studies by Dr Kelly Condon at James Cook University, the vaccine has been shown to be >90% effective. Vaccinated juveniles have also been purchased by Noosa Ecomarine and the vaccine has also coincided with substantially reduced mortalities by nodavirus in their recirculating aquaculture facility.
Final Report • 2023-02-01 • 18.85 MB
2018-098-DLD.pdf

Summary

Rocky Point Aquaculture in southeast Queensland experienced a disease outbreak in cage-reared giant grouper (Epinephelus lanceolatus) in late summer and autumn 2018 resulting is severe losses caused by a Betanodavirus. Following a request from the farm owner, Serena Zipf in July 2018, Dr Andrew Barnes from The University of Queensland, designed and built a recombinant protein subunit vaccine against the Betanodavirus and supplied the construct and production outline to Tréidlia BioVet Pty Ltd (Sydney) for manufacture in September 2018. Under direction from Dr Matt Landos and Dr James Fensham (Future Fisheries Veterinary Services) and with permission from Dr Richard Knuckey (General Manager, The Company One), juvenile grouper were vaccinated under a research permit from the Australian Pesticides and Veterinary Medicines Authority (APVMA) at The Company One (TCO) Cairns hatchery in October 2018, and shipped for grow-out at Rocky Point Aquaculture in December 2018, less than 6 months after concept discussion. Vaccination coincided with a reduction in the severity of outbreaks and from mid-2019 to 2022 the farm was free from nodavirus outbreaks. In summer 2022, a persistent low-level  outbreak was recorded at the farm in vaccinated fish, although again well below the mortality levels of the 2018 outbreak. In controlled laboratory challenge studies by Dr Kelly Condon at James Cook University, the vaccine has been shown to be >90% effective. Vaccinated juveniles have also been purchased by Noosa Ecomarine and the vaccine has also coincided with substantially reduced mortalities by nodavirus in their recirculating aquaculture facility.
Industry
PROJECT NUMBER • 2018-097
PROJECT STATUS:
COMPLETED

Survey of Enterprise-level Biosecurity across the Australian Aquaculture Industry

The Australian Government Department of Agriculture and Water Resources (the department) commissioned the independent research company Instinct and Reason to conduct a survey aimed at farm owners/managers in the Australian aquaculture industry. The survey aimed to investigate the level of...
ORGANISATION:
Instinct and Reason
TAGS
Survey
Invasive Species
Disease
Biosecurity
Aquaculture
SPECIES
Southern Bluefin Tuna
Yellowtail Kingfish
Barramundi
Atlantic Salmon
Black Tiger Prawn
Industry
PROJECT NUMBER • 2018-094
PROJECT STATUS:
COMPLETED

CRC Northern Australia - Biosecurity in northern Australian prawn aquaculture

The objectives of the Cooperative Research Centre for Developing Northern Australia (CRCNA) Improving Biosecurity in Northern Australia prawn farms (A.3.1718113) were to obtain an overview of the pathogens and the level of protection provided by the current biosecurity practices that occur in prawn...
ORGANISATION:
Australian Prawn Farmers Association (APFA)
TAGS
Invasive Species
Husbandry
Disease
Biosecurity
Aquaculture
SPECIES
Black Tiger Prawn
Banana Prawns
Industry
PROJECT NUMBER • 2018-090
PROJECT STATUS:
COMPLETED

Improving early detection surveillance and emergency disease response to Pacific Oyster Mortality Syndrome (POMS) using a hydrodynamic model for dispersion of OsHV-1

Rapid predictive capability of viral spread through water during an aquatic disease outbreak is an epidemiologist’s dream, and up until now has not been achievable. A biophysical particle tracking model for Ostreid herpesvirus 1 microvariant (OsHV-1) that causes POMS was developed to determine...
ORGANISATION:
Department of Primary Industries and Regions South Australia (PIRSA)
TAGS
Oceanography
Mortality
Modelling
Fisheries Management
Ecosystem
SPECIES
Pacific Oyster
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