30 results
Industry
PROJECT NUMBER • 2008-758
PROJECT STATUS:
COMPLETED

Seafood CRC: Development of a genetic management and improvement strategy for Australian cultured Barramundi

The aim of this study was to review existing Barramundi-related genetic knowledge to identify relevant research and where the R&D gaps preventing instigation of Barramundi breeding programs presently exist. They identified and prioritised the research and steps that need to be taken to establish...
ORGANISATION:
Flinders University
SPECIES
Blank

SCRC: SCRC Honours Scholarship H4.6 Effect of temperature and plant protein inclusion on the growth factor gene expression in Yellowtail Kingfish (Jian Qin; Student Geoffrey Collins)

Project number: 2009-780
Project Status:
Completed
Budget expenditure: $0.00
Principal Investigator: Jian Qin
Organisation: Flinders University
Project start/end date: 20 Dec 2009 - 20 Dec 2010
Contact:
FRDC

Final report

ISBN: 978-1-925982-79-4
Author: Geoffrey M Collins
Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

Final Report • 2010-12-21 • 349.23 KB
2009-780-DLD-Hons.pdf

Summary

In this study the suitability of canola oil (CO) and poultry fat (PO) as alternatives to fish oil (FO) was assessed. Furthermore, a real-time RT-qPCR assay to detect hepatic insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-1 (IGFBP-1) was developed. Four isoproteic (450 g kg-1), isolipidic (250 g kg-1) dry extruded diets were formulated to contain PO or CO at 50 or 100% inclusion, as a substitute for FO. Yellowtail Kingfish growth and growth factor gene expression was compared with fish fed a diet containing 100% FO.

Two experiments were conducted separately at optimal (22 oC; 33 d) and sub-optimal (18 oC; 34 d) temperatures with mean initial fish weights of 95.6 ± 0.1 g and 101 ± 0.1 g respectively. At 22 oC Yellowtail Kingfish fed the 50% PO diet grew significantly larger (281.2 ± 1.2 g) than individuals fed the 100% FO diet (266.9 ± 5.9 g). Inclusion of CO at 50% produced equivalent weight gain to the 100% FO diet, however, 100% inclusion of CO produced poor performance in both experiments. Trends in gene expression reflected the response in weight gain to alternative lipids. A positive relationship between hepatic IGF-I mRNA levels and weight gain and also hepatic IGFBP-1 mRNA and weight gain was found. A positive correlation between hepatic IGF-I and IGFBP-1 mRNA levels was also found. Irrespective of diet, fish grew larger at 22 oC than at 18 oC. The findings of this research indicate that improved diet formulations for Yellowtail Kingfish may be achieved through the inclusion of alternative lipid sources and that IGF-I mRNA may be used as a rapid indicator of growth in this species.

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PROJECT NUMBER • 2008-742
PROJECT STATUS:
COMPLETED

SCRC: PhD: Processing of Sea cucumber viscera for bioactive compounds

Sea cucumbers are prolific producers of a wide range of bioactive compounds, which are potential sources of agrichemical, nutraceutical, pharmaceutical and cosmeceutical products. Sea cucumbers expel their internal organs as a defence mechanism called evisceration. We hypothesize that the reason for...
ORGANISATION:
Flinders University

The oceanography of a continental shelf section

Project number: 1981-035
Project Status:
Completed
Budget expenditure: $0.00
Principal Investigator: Geof Lennon
Organisation: Flinders University
Project start/end date: 28 Dec 1983 - 31 Dec 1983
Contact:
FRDC

Objectives

1. Observe the temperature and salinity structure along a section from the mouth of Spencer Gulf SA to the edge of the continental shelf at three-monthly intervals
2. Take water samples at selected locations
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Organisation