NCCP: Determination of the susceptibility of silver perch, Murray cod and rainbow trout to infection with CyHV-3
Minor use permit for oxytetracycline in non-salmonid finfish
Development of a national sector-specific biosecurity plan guideline and template for the farmed freshwater native finfish industry of Australia
Freshwater fish attracting structures (FAS): Evaluating a new tool to improve fishing quality and access to fisheries resources in Australian impoundments
Beyond engagement: moving towards a co-management model for recreational fishing in South Australia
Towards responsible native fish stocking: Identifying management concerns and appropriate research methodologies
Better understanding of the impacts of stocking was flagged as a key national issue by the recent FRDC sponsored workshop on fish stocking. Stocked fisheries now represent an alternative recreational fishery that simultaneously reduces fishing pressure on marine fish stocks and delivers considerable social and economic benefits to regional communities. An aging population and increased leisure time for workers will create additional demands for fishing in the future. The continued success of freshwater fish stocking in Queensland and other States is contingent on demonstrating it is sustainable under the principles of ESD. The impacts of fish stocking on recipient ecosystems and wild fish stocks are poorly understood and these activities have drawn adverse criticism from conservation groups and environmental management agencies.
On a national scale, the Department of the Environment and Water Resources are driving sustainability issues via the EPBC Act with stocking fish outside their native range being nominated as a threatening process. Within Queensland all native fish stocking applications are assessed on their risk to local aquatic communities; other States have similar procedures. However, the decision making processes are poorly supported by data related to the likely ecological risks of the proposed stocking activities. These data cannot be obtained solely from desk-top studies that draw largely on overseas literature. What is urgently needed is pertinent research, done in an Australian context, to address key data deficiencies including impacts of stocking fish outside their natural range, displacement of natural populations and loss of genetic diversity. To do this effectively and to promote “world’s best practice” in our stocking industry, the issues first need to be succinctly defined and then appropriate methodologies developed to address them in research projects.
Final report
Aquatic Animal Health Subprogram: Optimisation of PCR tests for diagnosis of megalocytivirus (gourami iridovirus) and cyprinid herpesvirus 2 (goldfish herpesvirus)
Megalocytivirus
In 2003 there was an outbreak of iridovirus in farmed Murray cod in Victoria that caused 90% losses. In 2004 these viruses were found in imported ornamental fish - gourami. These have established breeding populations in the wild in Australia and therefore may pose a threat to biodiversity in the Murray-Darling Basin and other systems in which Maccullochella sp. still exist. These viruses are not host specific and all Murray-Darling Basin sp. may be susceptible. Due to the risk to aquaculture, recreational fisheries and biodiversity, there is need to develop and validate diagnostic tests for GIV in order to enable rapid accurate detection of GIV, facilitate surveys of Murray cod and imported ornamental fish to improve risk assessment and conduct surveys of free-living and farmed fish in Australia. The specific tests required include a robust and accurate PCR, and cell culture. These tests need to be able to distinguish GIV from related iridoviruses such as RSIV.
CyHV-2 of goldfish
In the last 10 years imported goldfish numbers have continued to grow, in part, due to their competitive price. The imported goldfish have been subjected to the AQIS requirements for import certification and a 3 week holding period before release. Anecdotal evidence suggests that mortality rates of domestically produced goldfish increase when these fish are mixed in retail shops with imported goldfish. Investigations have revealed pathology consistent with CyHV-2. Imported goldfish may carry latent CyHV-2 infections. This project aims to provide the diagnostic capability and a sampling regime to further investigate these concerns.
The project relates directly to Strategies 1, 2 and 3 in Aquaplan 2005-2010 and meets key research area 7.2.1 in the FRDC Aquatic Animal Health Sub Program Research and Development Plan 2002-2008 (updated June 2005).
Final report
Development of a health management strategy for the silver perch aquaculture industry
Currently there are about 180 licensed silver perch growers in all states; however, only about a third of these are producing fish commercially. Although a small number of farms achieve high production rates, most farms are inefficient and not producing anywhere near their potential. Survival, growth and production rates are much lower, and FCR's higher than achievable with good husbandry and management. Fish are being lost from disease and poor water quality, and growth rates are perceived by some farmers to be "slow".
Consultation with industry has identified that research into winter diseases and health management is a high R&D priority.
Diseases, in particular those caused by infectious agents, are recognised as an important threat to the viability of finfish aquaculture. In 1996/97 a pilot monitoring program aimed at identifying diseases causing significant production losses in silver perch was conducted on a coastal zone farm in north-eastern NSW. Results suggested that growth rates were reduced by ecto-parasitic infestations and by adverse water quality conditions. More recently, in 1998 and 1999, there have been reports of serious disease problems that have caused significant losses on some silver perch farms. These have included regular outbreaks of fungal diseases during winter, particularly in the cooler, inland areas of eastern Australia. It appears that some, or most of these outbreaks are not just the result of poor husbandry. The fungal disease, winter saprolegniosis is a serious problem in the large channel catfish industry in the USA, and relatively new winter fungal diseases have been reported in freshwater fishes in other parts of the world. There is strong evidence of a similar, but currently undescribed winter fungal disease in silver perch. Clearly there is a need to describe the major diseases, including important emerging diseases, on silver perch farms and identify their causes. Cost-effective control and prevention measures can then be developed.
More broadly, as the industry matures, silver perch farmers are becoming increasingly aware of the importance of systematic, cost-effective measures aimed at reducing disease-related losses to acceptable levels. However, no such validated programs are currently available to the industry. To fill this vacuum, it is essential that "Health Management Programs" i.e. generic disease control and prevention programs, are developed, validated and extended to farmers. These programs can be modified to suit the needs of individual farms and integrated with routine management activities. On individual farms, the programs will comprise (a) broadly targeted measures based on established principles and aimed at general disease prevention, early detection and control, with (b) specifically targeted measures aimed at reducing losses caused by important diseases (e.g. winter diseases) occurring in the farm's geographic area.
The production capacity of silver perch (10 tonnes/ha/year), the established culture techniques, the large number of inefficient farms, and the ready availability of sites provide the basis for a dramatic increase in production of silver perch over the next 5 to 10 years. However, research to address the current disease problems is required to maximise the value of previous research and to enable the industry to realise its full potential.
Final report
The effect of barramundi Nodavirus on important freshwater fishes
Susceptibility of freshwater fishes to barramundi nodavirus.
- There is a need to address concerns about the risk of possible lethal transmission of barramundi nodavirus to freshwater fishes already under threat in their natural habitat.
- Important freshwater fishes will be exposed to the nodavirus from cell cultures to determine their susceptibility.
- By using the OVL isolation facility in Townsville, well outside the Murray-Darling region, there is minimal risk to the natural fish populations.
- Confirmation that barramundi nodavirus can cause lethal infections in freshwater fishes will strengthen the application of strict licence conditions on barramundi farming in southern Australia.
- Confirmation that barramundi nodavirus does not affect freshwater fishes will possibly allow expansion of the barramundi farming into regions needing new sustainable economic development.
A barramundi infection model.
- To establish a realistic virus dose and route of infection for the challenge trials, an infection model using barramundi will be developed.
- OVL has ready access to barramundi larvae and fry of all ages.
- The model is also necessary to help quantify the effect of virus exposure to disinfectants and different environmental conditions.
What is the viability of barramundi nodavirus?
- Fish health management requires good information on how to effectively decontaminate facilities following outbreaks of VNN.
- Knowledge of the persistence of barramundi nodavirus in the environment will allow fisheries managers to decide on effective conditions for barramundi farming licences that minimise the risk of transferring virus outside the culture facility.
Sensitivity of the cell culture isolation system.
- While sensitive detection tests are available, the barramundi cell line offers a more practical diagnostic method that can be used by any laboratory with cell culture/ virology capability.
- An evaluation of the cell culture isolation system’s ability to detect virus in carrier (no disease) fish, and standardization of the cell culture presentation, is required before the method can be recommended.
Final report
This project has confirmed the knowledge that management of barramundi translocations outside their natural range requires fisheries authorities take into account the risk that barramundi nodavirus may lethally infect native freshwater fishes. The project has shown barramundi nodavirus can multiply and spread throughout the body of freshwater fishes, and that the spread of infection from fish to fish is a possibility in freshwater.
The project has created knowledge on the Australian application of a sensitive molecular detection test for nodavirus in healthy fishes. This knowledge has lead to industry and government support for further research on diagnostic test development for nodaviruses which will lead to a national Standard Diagnostic Procedure. Further, this knowledge has led the barramundi hatchery sector to support a research project applying the molecular detection test to screen captive barramundi breeders for nodavirus with the aim to produce nodavirus-free barramundi fry.