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Industry
PROJECT NUMBER • 2021-061
PROJECT STATUS:
COMPLETED

Development of a guideline to investigate and understand disease outbreaks of unknown cause

This handbook presents guidelines that have been developed to use in the event of a disease outbreak in aquatic animals including finfish, molluscs and crustaceans. For the purposes of these guidelines, in the context of an outbreak, the term “disease” will be used for any event where...
ORGANISATION:
Agriculture Victoria
Industry
Environment
PROJECT NUMBER • 2001-214
PROJECT STATUS:
COMPLETED

Aquatic Animal Health Subprogram: development of a disease zoning policy for marteiliosis to support sustainable production, health certification and trade in the Sydney rock oyster

The edible oyster industry in Australia is currently valued at around $62.5 million annually of which rock oyster production accounts for approx 56%. For the industry to survive in the long-term requires the ability to service what may become a premium domestic market demanding a high quality...
ORGANISATION:
Queensland Museum
TAGS
Environment
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Seafood CRC: discovery and manipulation of Neoparamoeba perurans aquaporins as a means to treat amoebic gill disease (AGD)

Project number: 2010-731
Project Status:
Completed
Budget expenditure: $53,250.00
Principal Investigator: Mat T. Cook
Organisation: CSIRO Oceans and Atmosphere Hobart
Project start/end date: 30 Apr 2010 - 29 Nov 2010
:

Need

Cumulative data from other parasites suggests that blocking of Aquaporin channels (AQPs) may provide a suitable target for the use of pharmaceuticals to control these diseases. Neoparamoeba share many similarities with other pathogens. However, there is a need to correctly identify the Neoparamoeba AQPs at the molecular level, to undertake preliminary studies to show that these AQPs can be blocked preferentially (without affecting Atlantic salmon AQPs), and to show that block of the parasite AQPs results in inhibition or death of the Neoparamoeba. Central to this work and indeed to any further AGD research is a need to develop low cost, rapid and reliable assays of salmon gill cell – Neoparamoeba interactions. These in-vitro assays would lead to real dollar savings in the search for alternative AGD treatments by providing high throughput capability to assess multiple treatment options (pharmaceuticals, vaccines, chemicals). They provide a means of investigating amoebae – gill interactions as well as differential (pathogen versus host) effects of treatments. Dr Benita Vincent at CSIRO has begun pilot development of these assay systems with good early success, however, further resources are required to see this work to fruition.

Objectives

1. To clone and sequence Neoparamoeba perurans aquaporins
2. To screen a library of known aquaporin blockers against both Neoparaomoeba perurans and Atlantic salmon aquaporins in an in-vitro Xenopus oocyte system
3. To refine and establish routine protocols for an Atlantic salmon gill cell line and in vitro assay for examining amoeba-gill and treatment interactions
4. To take the best identified aquaporin blockers (from objective 2) and test these in vitro to determine if they kill or inhibit Neoparamoeba perurans without affecting Atlantic salmon cells
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