Detecting paralytic shellfish toxins in oysters - initial assessment of AquaBC rapid test kit

Project number: 2023-103
Project Status:
Current
Budget expenditure: $29,845.00
Principal Investigator: Andreas Seger
Organisation: Institute for Marine and Antarctic Studies (IMAS) Hobart
Project start/end date: 30 May 2024 - 20 Feb 2025
Contact:
FRDC

Need

Reliable detection of marine biotoxins is a critical requirement for any effective biotoxin monitoring program, requiring any analytical technique to be properly validated. The Neogen rapid test kit for the detection of paralytic shellfish toxin (PSTs) was successfully validated in both single lab and inter-lab validations for use in oysters, offering rapid (within 20 min) on farm results. The test was used in Tasmania to reduce business risk, (i.e. frequent testing of shellfish that can rapidly accumulate PST within a week) and employed in South Australia for regulatory purposes (low frequency of PST detection in this region).

A key factor influencing the suitability of antibody based rapid test kits is the PST profile present in the sample to be analysed. The term PST profile describes the relative concentrations of different PST analogues that might be present in each seafood sample. These profiles differ between toxic algal species, different seafood species and their tissues. Not all PST analogues are equally detected by the antibodies of different test kits (quantified as the % cross-reactivity). These cross-reactivities are critical for ensuring reliable detection across different combinations of PST analogues that might be present in shellfish.

To determine if the AquaBC rapid test kit is suitable replacement for routine monitoring, a full validation for each seafood tissue matrix would normally be conducted to determine the probability of detection curve (probability of detection across a range of PST concentrations) for multiple PST profiles, ideally followed by an inter-laboratory validation (as was conducted for the Neogen test). However, a full validation study requires repeat testing of hundreds of samples and is therefore expensive. From our previous work with the Neogen test kit, we have identified some key performance indicators that would allow for a quick initial assessment of the new AquaBC test kit, using much reduced sample numbers. These small pilot trials would include testing the most commonly encountered PST profiles (i.e. which PST toxin analogues are present) and their relative concentrations (i.e. can we reliably detect the presence of PST at the regulatory level without obtaining too many positive results at PST levels below concern?). Rather than conducting a full validation, this project will provide an initial assessment of these parameters in regard to the PST profiles commonly encountered in TAS, SA and NSW. Should this assessment be positive, a full follow up validation may be recommended.

Objectives

1. Review & identify different PST profiles that may be encountered in TAS, SA and NSW oysters.
2. Challenge the AquaBC test kit against a range of different PST concentrations and profiles to provide an initial assessment of its suitability for detecting PST in Australian oyster tissues.

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Fish toxin tests to track ciguatera risk

Published: 14 April 2023 Updated: 1 July 2025
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DATE 28 Apr 2023
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Commercial and recreational fishers have contributed hundreds of fish samples to help develop new ways to detect ciguatera toxins, as part of FRDC funded food safety research. 

By Catherine Norwood

 

Risk profile for paralytic shellfish toxins in Tasmanian Periwinkles

Project number: 2021-108
Project Status:
Current
Budget expenditure: $180,504.00
Principal Investigator: Andreas Seger
Organisation: Institute for Marine and Antarctic Studies (IMAS) Hobart
Project start/end date: 28 Apr 2022 - 29 Jun 2025
Contact:
FRDC

Need

Recurrent harmful algal blooms in Tasmanian waters (Gymnodinium in the South and Alexandrium on the East Coast) have impacted Oyster, Mussel, Abalone and Rock Lobster industries, necessitating the implementation of biotoxin monitoring programs and harvest closures during high risk periods. It is currently unknown whether Periwinkles can accumulate paralytic shellfish toxins (PST) from Tasmanian microalgal blooms and a conservative risk management approach has been taken thus far to protect both public health and market access.

Unlike bivalve shellfish, Abalone and Rock Lobster, there is currently no clearly defined biotoxin management plan for Periwinkles in Tasmania. As grazers, Periwinkles are loosely grouped with Urchins and Abalone, the latter of which are a proven PST risk. This Abalone biotoxin sampling plan is triggered when Tasmanian Shellfish Market Access Program (ShellMAP) closes bivalve harvest areas due to PST risk. In a conservative approach, the current recommendation is PST analysis of 20 pooled Periwinkles per Abalone block on at least a monthly basis to maintain market access. This presents a considerable cost burden to the small dive fishery and interrupts harvest operations, as PST sampling of Periwinkles is required if elevated PST are detected in Abalone, which are known to hold onto PST for longer time periods in between blooms (years).

To date, the vast majority of Periwinkle samples that have been analysed for PST were collected from weak or no bloom years or regions. Evidence from French tank trials exposing the common European Periwinkle (Littorina littorea) to Alexandrium minutum cysts containing PST showed significant toxin uptake (0.2 mg PST/kg) within six days of exposure (Neves et al, 2015). It remains unknown whether commercially harvested Tasmanian Periwinkles (Lunella undulata) are at risk of PST accumulation and if the current conservative monitoring approach is commensurate to risk. In such scenarios, risk managers will commonly outsource a preliminary risk assessment (known as a risk profile). Risk profiles are an important tool for risk managers and industry. They provide a summary of all information pertinent to food safety associated with the specific hazard/food combination. The purpose of a risk profile is to assist initial risk management activities, such as identifying future actions required (if any), and the options for food safety management programs. They also inform the level of resourcing required to control the hazard/food pairing.

This project will deliver an urgently required risk profile for PST in Periwinkles to inform future actions (if any) and provide options for cost-effective food safety management programs. This profile will:
1. Determine the risk of PST accumulation in Periwinkles in Tasmania as a result of harmful microalgal blooms during high risk exposures at peak algal bloom densities in the field and in tank trials.
2. Outline potential management options and knowledge gaps, should risk management be necessary. This includes an assessment of whether the bivalve PST regulatory limit is appropriate to use in Periwinkle risk management (currently based on shellfish serving size of 100-400g) and the spatial variability associated with sampling.

References
Neves, Raquel AF, et al. "Responses of the common periwinkle Littorina littorea to exposure to the toxic dinoflagellate Alexandrium minutum." Journal of Molluscan Studies 81.2 (2015): 308-311.

Objectives

1. Determine whether Tasmanian Periwinkles can bioaccumulate PST from Alexandrium and/or Gymnodinium microalgal blooms during both field and laboratory exposures.
2. Identify the most suitable size of biotoxin management zones for Periwinkles.
3. Assess whether the use of the bivalve PST regulatory limit is appropriate for Periwinkles by conducting a literature search and consumption survey.
4. Generate a risk profile for PST in Tasmanian wild caught Periwinkles and provide guidance for risk management.