41 results
Industry
PROJECT NUMBER • 2018-086
PROJECT STATUS:
COMPLETED

Assessment of the capability of Shrimp MultiPath White Spot virus tests: A multiplexed screening platform for pathogen diagnostics in prawns

This report summarises the development and performance assessment of novel PCR based assays to monitor and detect the microsporidian Enterocytozoon hepatopenaei (EHP) in Penaeid shrimp samples using a cost-effective and high-throughput approach. The original Project objective was to...
ORGANISATION:
Genics Pty Ltd
Industry
PROJECT NUMBER • 2018-099
PROJECT STATUS:
COMPLETED

Research to provide data to support application for a minor use permits for chemicals including trichlorfon, chlorine, copper sulfate, hydrogen peroxide, benzylkonium chloride for control of disease including White Spot Syndrome Virus (WSSV) in Australian prawn farms.

The project involved generation of chemical data packages through review and aggregation of public domain documents for each chemical (trichlorfon, hydrogen peroxide, sodium and calcium hypochlorite, copper sulphate and benzalkonium chloride). This facilitated identification of data gaps requiring...
ORGANISATION:
Future Fisheries Veterinary Service Pty Ltd (FFVS)

Water disinfection for influent water biosecurity on prawn grow-out farms

Project number: 2021-026
Project Status:
Current
Budget expenditure: $125,430.00
Principal Investigator: David Mann
Organisation: Department of Agriculture and Fisheries Bribie Island
Project start/end date: 23 Jan 2022 - 29 Nov 2022
:

Need

• Coping with the potential persistence of WSSV in Australian waters
It is important for farms in the WSD risk region, as well as those outside it, to have access to the necessary tools and reliable information that enables them to implement highly effective biosecurity measures when they become necessary.

• Industry white spot disease preparedness
Chemical treatment to remove residual vectors and/or destroy the pathogen is currently the only practical approach with potential to adequately reduce WSD biosecurity risk associated with influent farm water. When assessment indicates a high disease risk, farms will need to be confident that their biosecurity measures will be immediately effective.

• Appropriate guidelines for using trichlorfon
Currently there is no locally generated guideline for achieving effective application of trichlorfon that is based on directly applicable experimental data and prawn farm experience. The industry needs a set of guidelines that expand upon the basic APVMA use conditions and define the environmental factors and application parameters that will provide the greatest protection for Australian farm conditions.

• Gaps in information currently available
The available information does not provide substantiated details of trichlorfon treatment methods and outcomes. The Australian industry needs validated treatment methods and their effectiveness and biosecurity outcomes verified.

• Constraints on trichlorfon use during production
Using trichlorfon to treat top-up and exchange water during the production cycle is problematic for some farms due to the 12 day conditioning period required to ensure toxic residues are below the detectable limit before stock is exposed to the water. The potential to manipulate water quality parameters to accelerate residue degradation could make treatment throughout the cycle a more practical option.

• Continuation of permit to use trichlorfon
Documented evidence of outcomes and impacts of trichlorfon use on farms is needed to support an APVMA application to extend its use beyond the current MUP period ending 31 December 2021.

Objectives

1. Determine the efficacy of trichlorfon treatment for removal of crustaceans from farm influent water, and the rate of toxic residuals degradation, under a range of treatment circumstances experienced on prawn farms.
2. Provide the prawn grow-out sector with a practical guide for the optimal use of trichlorfon as a water biosecurity method.
3. Provide data for APVMA registration of trichlorfon use, including practical methods for reducing the withholding period for trichlorfon treated water.
Industry
Industry
Industry
PROJECT NUMBER • 2017-238
PROJECT STATUS:
COMPLETED

Water treatment to control influent water biosecurity risk on Australian prawn farms. Effectiveness and impacts on production ponds.

This project assessed the performance of mechanical filtration as a means by which Australian prawn farmers could lower the risk of disease agent transfer into farms by selective removal of disease hosts and other vectors naturally present in farm source water. The project sought to provide...
ORGANISATION:
Department of Primary Industries (QLD)

Clarence Riven Green Prawn Market Diversification Assistance Measures

Project number: 2023-093
Project Status:
Current
Budget expenditure: $295,000.00
Principal Investigator: Jayne M. Gallagher
Organisation: Honey and Fox Pty Ltd
Project start/end date: 17 Mar 2024 - 1 Jun 2025
:

Need

The Clarence River Region is known for its high-quality prawn markets. The region was kept profitable during COVID restrictions due to a significant portion of the fisher directing its product to the highly profitable bait market. This diversification away from the consumer market has ensured stability for the region. Many fishing businesses pivoted their strategies to meet this new buyer to the region. The loss of the uncooked prawn market in the Clarence region will therefore destabilize the industry.
The restriction of uncooked prawn trade therefore must be addressed through market research, diversification and activation.
However, there is immediate need for alternative markets so an intense focused market activation and access is the key.
PFA has identified key market persons that can create links between industry and high-end chefs to:
1. Identify alternative market uses that fall within the quarantine requirements
2. Start immediate market trials and activation
3. Review online presence and build industry skills to improve online presence for sales
• It is intended that this will lead into immediate supply agreements to these alternative markets that will in turn remove burden on the existing cooked prawn market

Objectives

1. Trial traceability and authenticity technologies to assess feasibility of creating a bio-secure marketing platform for prawns from white spot affected areas
2. Develop market communication and education materials to support market diversification efforts of seafood businesses impacted by the white spot virus related restrictions in the Clarence River region

Australian Prawn Farms - Emerging disease investigation

Project number: 2018-166
Project Status:
Current
Budget expenditure: $180,960.00
Principal Investigator: Matthew A. Landos
Organisation: Australian Prawn Farms Pty Ltd
Project start/end date: 3 Feb 2019 - 30 Nov 2019
:

Need

Bacterial cultures from sick PL's from crashing hatchery tanks did not reveal a common pathogen across the multiple dying hatchery tanks.
Understanding the cause of the substantial hatchery PL mortality could be of value to all hatcheries across the prawn farming industry. Intermittent tank crashes are reported at most hatcheries over their history of operation.
With use of new diagnostic tools, such as next Generation sequencing, exploration for novel pathogens can be commenced using stored material from the hatchery outbreaks.
The further investigation of the hatchery and pond mortalities, offers the opportunity to better define the factors associated with the syndrome, thereby informing management measures for future seasons at this farm, and for the rest of the prawn farming industry.

Objectives

1. Describe potential pathogens in six batches of preserved PL's from sick APF hatchery tanks from 2018 season
2. Develop qPCR for bacterial toxin genes identified in NGS analysis of bacterial cultures collected from APF hatchery water during 2018 season
3. Determine the presence of putative bacterial toxins in prawn larvae samples from sick APF larvae tanks during 2018 season
4. Identify useful post-stocking sampling methods for PL's to monitor health status
5. Preservation of samples from three trial stocking ponds to provide temporally relevant samples for subsequent analysis if warranted by low survival determination at 5-8 weeks.
6. Determine the cause(s) of low survival in experimental prawn ponds, should it occur, using range of diagnostic tests and epidemiological assessment of risk factors
7. Levels of agri-chemical pollutants in source water entering ponds will be measured and their effects on prawn larval health considered
8. Develop recommendations for future operation of hatchery and ponds at APF

Final report

ISBN: 978-1-64713-669-7
Authors: Dr James Fensham Dr Matt A. Landos Mr Matt West Dr Ian Anderson Dr Charles Caraguel
Final Report • 2020-01-01 • 2.09 MB
2018-166-DLD.pdf

Summary

This project was developed by Future Fisheries Veterinary Service (FFVS) in collaboration with Australian Prawn Farms (APF), James Cook University (JCU) Aqua Path laboratory, Queensland Biosecurity Sciences Laboratory (BSL) and the University of Adelaide (UofA). Unprecedented mortality events occurred in a commercial Australian prawn industry hatchery and grow-out farm between August 2018 and January 2019. Initial investigations did not identify the consistent involvement of an infectious agent in particular. A range of potential risk factors appeared to be associated with tank- and pond-level mortality events, however subsequent mitigation of these in the hatchery, did not appear to fully resolve the mortalities. The chronology of outbreaks within the hatchery and soon after pond stocking raised alarm that this could be an Australian case of Early Mortality Syndrome. Further investigation was warranted to identify if emerging infectious agents may be involved and to determine evidence-based control and prevention methods accordingly. During 2019, a prospective grow-out pond trial and retrospective epidemiological analysis of farm data were performed, to identify potential causative agents and/or risk factors associated with the mortality events. The project outputs will help shape recommendations for reducing disease risks, improve hatchery and grow-out farm practices, and further refine disease investigation approaches. The project was funded through the Fisheries Research and Development Corporation (FRDC).
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