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Industry
Industry

Abalone Viral Ganglioneuritis (AVG) R&D Needs Workshop

Project number: 2021-076
Project Status:
Completed
Budget expenditure: $29,939.11
Principal Investigator: Craig Fox
Organisation: Abalone Council Victoria Inc (ACVI)
Project start/end date: 31 Oct 2021 - 17 Mar 2022
Contact:
FRDC

Need

Abalone Viral Ganglioneuritis (AVG) disease is caused by a herpesvirus (infection with abalone herpesvirus, AbHV) and was first detected in Victoria in December 2005. The last recorded instance of this disease in Victoria was in January 2010 at Cape Otway.

The disease was again confirmed in the Cape Nelson area on 2 May 2021. Since then, further detections have been made at several locations and has been confirmed in Victoria as far east as Cape Otway and as far west as the Discovery Bay Marine Park.

While the virus is active there is a need for the Victorian wild catch abalone fishers to urgently review past research, to inform a gap analysis and further research.

This review will support those involved in the outbreak and inform fellow members nationally with a summary of past research and a way forward with other research for up-to-date biosecurity protocols, surveillance, and diagnostic tools to aid rapid detection to future proof the industry. Relevant industry, government representatives and stakeholders will be kept informed and asked to comment and contribute issues and ideas for a cooperative structured way forward to build a common framework on which to establish a set of key actions and or priorities. At the end of a staged process a workshop will be organised to evaluate and reassess research needs, assess and implement research findings and if further gaps are identified develop a framework to action.

Objectives

1. Conduct an analysis of past research on AVG, create a plain English summary and identify knowledge and research gaps that can be evaluated nationally by key stakeholders
2. Hold a workshop that will be a mixture of face to face and virtual so that key stakeholders can attend and participate in evaluation of suggested research projects identified
3. Prepare and provide a final report that clearly articulates research priorities for AVG in the short term and long term

Workshop

ISBN: 978-0-6455081-0-9
Authors: Craig Fox Harry Peeters Ian Knuckey Helen Burvill
Workshop • 2022-07-01 • 1.03 MB
2021-076-DLD.pdf

Summary

Following Abalone Viral Ganglioneurits (AVG) re-emerging in the Western Zone of the Victorian Abalone Fishery in early May 2021 it was decided that a workshop needed to be convened as soon as possible with relevant stakeholders to evaluate past research and understand research gaps while the virus is active, and samples of active material can be collected and stored in a secure laboratory in readiness for future testing. Stakeholders from wild catch Victoria, South Australia, Tasmania, and New South Wales; farmed abalone sector; government and research agencies and universities gathered for a 1-day workshop, held in Queenscliff on 14th December 2021 to identify and prioritise future research and development priorities.

Investigating the use of a remote operated underwater vehicle (ROUV) for surveying abalone on reefs in the Western zone abalone fishery, Victoria.

Project number: 2020-125
Project Status:
Current
Budget expenditure: $160,000.00
Principal Investigator: Harry F. Peeters
Organisation: Western Abalone Divers Association (WADA)
Project start/end date: 3 Jun 2021 - 21 Feb 2022
Contact:
FRDC

Need

Provide a tactical and strategic response to the recent outbreak. Develop a cost effective assessment tool to validate the spread of the virus and build a long term mechanism to undertake fisheries independent surveys.

Objectives

1. To validate a remotely operated underwater vehicle (ROUV) survey tool
2. Assess options to integrate video from ROUV technology and other sources with existing diver data to develop fisheries independent surveys
3. To validate non surveyed abalone populations
Industry
Environment
PROJECT NUMBER • 2020-065
PROJECT STATUS:
CURRENT

Indicators for density and biomass of exploitable abalone – developing and applying a new approach

The objectives of this project are to use the Victorian Western Zone (WZ) abalone fishery to develop and evaluate a new approach to calculating abalone density and biomass indicators from the combination of three information sources. The three information sources are: i. ...
ORGANISATION:
Western Abalone Divers Association (WADA)
Industry
PROJECT NUMBER • 2019-204
PROJECT STATUS:
COMPLETED

Australian Abalone Growers Association Strategic R&D Plan 2020-2025

AAGA established its first strategic plan in 2015 for the period 2015-20. AAGA members now wish to develop a new strategic plan to inform further investment in our sector for the period 2020 to 2025 and beyond. AAGA and FRDC recognise the need for the sustainable development of the...
ORGANISATION:
Ridge Partners

The use of dietary intervention with Ulva to improve survival associated with the incidence of summer mortality on farmed Abalone

Project number: 2019-156
Project Status:
Current
Budget expenditure: $157,724.00
Principal Investigator: David A. Stone
Organisation: University of Adelaide
Project start/end date: 6 Feb 2020 - 29 Nov 2020
Contact:
FRDC

Need

To improve industry productivity the AAGA need to identify new and improved diets to reduce the impact of summer mortality on the survival of cultured abalone in Australia.

Objectives

1. The objective of this project is to use dietary intervention using dried Ulva meal to ameliorate the effects of summer mortality on the survival of greenlip abalone (H. laevigata) and tiger abalone (H. laevigata × H. rubra) cultured under commercial conditions during the summer production cycle.

Final report

ISBN: 978-1-876007-39-3
Authors: David A.J. Stone Joel Scanlon James O. Harris Matthew Bansemer Gordon S. Howarth and Nicole L. Thomson
Final Report • 2022-05-01 • 1.59 MB
2019-156-DLD.pdf

Summary

A research priority identified by the Australian Abalone Growers’ Association (AAGA) in 2019, prior to the commencement of the project, was to improve the survival of commercially cultured abalone during periods of high summer water temperatures (> 22°C). This research aimed to address this need. This project was developed by SARDI in collaboration with members of the Australian Abalone Growers’ Association (AAGA), Aquafeeds Pty Ltd and researchers from Flinders University and The University of Adelaide. The primary aim of the project was focused on evaluating the effects of feeding a diet containing 10% dried Ulva sp. meal on improving the survival of Australian Greenlip (Haliotis laevigata) and Tiger Abalone (H. laevigata × H. rubra) cultured throughout summer to autumn in two separate farm trials. Histopathological alterations of selected tissues of the digestive tract and gills of abalone were also assessed for each trial during this period. The secondary aim of the project evaluated the growth, feed utilisation and production cost of abalone for each trial. This project built on research that reported that dietary intervention, utilising dried Ulva sp. meal in a practical commercial formulated feed, reduced mortality in cultured Greenlip Abalone (H. laevigata) associated with elevated summer water temperatures (26°C) under laboratory conditions (Lange et al., 2014).
Abalone in both farm trials experienced mild summers and water temperatures. However, the JTA Avalon trial experienced several instances where water temperatures exceeded 22°C during January and February. The maximum water temperature experienced during the Yumbah Narrawong trial was 22.5°C in early January and remained relatively low compared to those recorded at JTA Avalon. Summer mortality was observed during the farm trial at JTA Avalon, whereas water temperatures were too low during the Yumbah Narrawong trial to induce summer mortality. The dietary inclusion of 10% dried Ulva sp. meal did not result in any significant improvement in survival during either farm trial. In fact, survival appeared to be lower during both farm trials when 10% dried Ulva sp. meal was included in the diets (JTA Avalon survival: commercial diet survival 96.4% vs. 93.4% for the Ulva diet; Yumbah Narrawong survival: commercial diet 98.1% vs 97.5 % for the Ulva diet).
The dietary inclusion of 10% dried Ulva sp. meal did not appear to lead to any significant alteration in digestive tract or gill structure. Heat stress, in combination with commercial culture stressors, appeared to be a factor associated with histopathological alterations observed in abalone during the study, which were chiefly noted in the gill leaflet tips of Tiger Abalone. Gill leaflet tip damage scores appeared to be useful indicators of damage associated with the combination of culture stressors and heat stress in abalone.
In the Yumbah Narrawong trial, which was carried out during mild summer growing conditions, the dietary inclusion of 10% dried Ulva sp. meal improved growth (SGR increased by 9.6% combined with economically irrelevant differences in survival), feed and nutrient utilisation and live biomass yield for a mixed population of Tiger and Greenlip Abalone and compared to the commercial diet ultimately led to a $729 or 8.4% increase in basic sales revenue per slab tank for the 166-d trial period. Unfortunately, due to logistical constraints due to COVID-19, growth performance data were not available from JTA Avalon trial.
Overall, the dietary inclusion of 10% dried Ulva sp. meal did not lead to improvements in survival of Tiger or Greenlip Abalone cultured during summer. Based on the data obtained from the Yumbah Narrawong trial, improvements in growth performance (~9.6% improvement in SGR), feed and nutrient efficiency and basic sales revenue were obtained when abalone were cultured under mild summer growing conditions and fed the Ulva diet compared to the commercial control diet.
Data produced by this project support the concept that diets for improved abalone production should not be formulated on a least cost basis, but rather on an ingredient quality, and abalone and economic performance basis. Feeds formulated on this basis may support improved profitability.

Application of a machine learning approach for effective stock management of farmed abalone

Project number: 2019-151
Project Status:
Completed
Budget expenditure: $115,649.00
Principal Investigator: Jan Strugnell
Organisation: James Cook University (JCU)
Project start/end date: 14 Sep 2020 - 30 Aug 2022
Contact:
FRDC

Need

Determining the number and size distribution of abalone present at various stages of production is critical information for effective stock management. Currently the Australian abalone aquaculture industry spends in the order of $25,000 per annum, per farm, gathering this information by hand. However, the resulting data is of mediocre quality, is limited in its scope, and collecting the data causes stress to the animals (as it is removed from the water) which can compromise growth and survival. Automated counting and measuring of abalone will increase farm efficiency and productivity in the short term and, in the longer term, will provide an advanced platform for further R & D improvements including accurate data collection during experimental trials (e.g. feeds, temperature). Artificial intelligence and machine learning has now matured to a point that accurately counting and measuring abalone is possible using this approach, however specific application to the abalone industry is yet to be achieved. This project would involve the development, training and validation of a machine learning model to identify, segment and measure quantitative abalone traits in production systems and, render the product data to be accessible and applicable for farmers.

Objectives

1. To develop and implement artificial intelligence as a method for accurately measuring and counting abalone at nursery, weaning and grow out.

Final report

ISBN: 978-0-6457422-6-8
Authors: Kurt Schoenhoff Kyungmi Lee Jason Holdsworth Hemmaphan Suwanwiwat Jan Strugnell Ickjai Lee
Final Report • 2022-12-01 • 3.90 MB
2019-151-DLD.pdf

Summary

This report provides detail on the development of a machine learning tool as a method for counting and measuring abalone at various stages of production. The study was carried out on hybrid abalone with ~2000 images (nursery, weaner and growout stage) collected from Southern Ocean Mariculture and Yumbah. A deep learning based method for counting abalone (nursery stage) and counting and measuring abalone (weaner and growout stages) was successfully developed and trained. A user-friendly cross platform software application was developed to enable use of the tool by abalone farmer. The tool should make stock assessment faster, more accurate and provide less stress to farmers. The project was carried out from 2019-2022 by the James Cook University Information Technology team in Cairns (Kyungmi Lee, Ickjai Lee, Jason Holdsworth, Hemmaphan Suwanwiwat, Kurt Schoenhoff) in with contributions from Phoebe Arbon (who held a 2020 Science and Innovation Award for young people in agriculture, fisheries and forestry with a similar time frame) and Jan Strugnell.
Industry
PROJECT NUMBER • 2019-147
PROJECT STATUS:
COMPLETED

Risk factors and management strategies associated with summer mortality in Australian abalone

In this project, we reviewed the scientific literature and collaborated with Australian abalone growers to develop a case definition for summer mortality. The case definition developed for summer mortality is as follows: i. Chronic mortality of unknown cause (if in doubt take this to mean...
ORGANISATION:
University of Adelaide
Industry
PROJECT NUMBER • 2019-084
PROJECT STATUS:
COMPLETED

Seafood Marketing Symposium 2020

The Queensland Seafood Marketing Association (QMSA) produced a series of 8 Webinars with the focus on seafood marketing information. This was to coincide with the launch of Great Australian Seafood television campaign in late 2020, empowering industry to to take advantage of this campaign and apply...
ORGANISATION:
Queensland Seafood Marketers Association Inc (QSMA)
Environment
PROJECT NUMBER • 2019-038
PROJECT STATUS:
COMPLETED

Abalone diver observation collection, analysis and reporting system for improved management decision making

This project was an industry-led and highly collaborative project to develop a rigorous and cost-effective electronic platform to gather abalone divers’ observations of abalone stocks in situ that could be useful to inform the management of Australian abalone fisheries.
ORGANISATION:
Abalone Council Victoria Inc (ACVI)
Adoption
PROJECT NUMBER • 2018-180
PROJECT STATUS:
COMPLETED

Benchmarking for health and productivity in aquaculture

Benchmarking is a form of evaluation undertaken by comparing a measure with a standard. With its widespread adoption across many industries, benchmarking was identified as an important area for development in aquatic industries through the national strategic plan for aquatic animal health (AQUAPLAN...
ORGANISATION:
Agriculture Victoria

Population genomic assessment of Australian Blacklip Abalone for abalone viral ganglioneuritis (AVG) resistance

Project number: 2018-057
Project Status:
Completed
Budget expenditure: $165,700.00
Principal Investigator: Adam Miller
Organisation: Deakin University Warrnambool Campus
Project start/end date: 14 Jul 2019 - 29 Oct 2020
Contact:
FRDC

Need

AVG remains the greatest threat to the economic viability and stability of the abalone industry in south-eastern Australia. As a consequence, there is an urgent need for strategic research aimed at determining the likely vulnerability of fisheries to future AVG outbreaks and providing managers with the necessary tools for biosecuring wild and farmed stocks at regional, state and national scales.

The fact that some animals from AVG affected wild stocks survived the disease outbreak suggests they were either fortunate enough to have avoided coming into contact with the virus or are genetically resistant to the disease. A research program aimed at characterising AVG resistance in Australian wild abalone fisheries is expected to provide benefits to wild and farm fisheries at a national scale. If AVG resistance is present in wild abalone stocks, and its genetic basis can be characterised, there will be unique opportunities to:

1) Rapidly and cost-effectively screen stocks across all wild fisheries to determine the spatial prevalence of resistant genotypes and to gain an understanding of how biosecure wild abalone stocks are likely to be in the event of AVG re-emergence
2) Biosecure wild stocks through the movement of animals from ‘AVG resistant’ to ‘AVG vulnerable’ stocks as part of future restocking and translocation activities
3) Biosecure farm stocks across all states of Australia through the establishment of an AVG resistance breeding program

This project involves a direct partnership with AAGA, ACA and VFA and is expected to provide much needed insights into the vulnerability of abalone stocks to future AVG outbreaks, and the tools needed to bolster the biosecurity of wild and farmed abalone stocks. As a result, this project has the potential to dramatically improve the economic viability of this rapidly expanding industry.

Objectives

1. This project will leverage the support of the abalone wild harvest and aquaculture industries, and the Victorian state government to determine if genetic resistance to the AVG virus persists in Australian wild blacklip abalone fisheries. This project will adopt state-of-the-art population genomic technologies to contrast genomic profiles of 350 individual abalone distributed across AVG affected and unaffected blacklip abalone stocks from Victorian Western and Central zone fisheries. Genomic variants differentiating animals from AVG affected and unaffected source stocks are expected to be indicative of a selective sweep and AVG resistance. Genomic variants associated with AVG resistance will act as the necessary markers for surveying the virus resilience/vulnerability of wild fishing stocks, and biosecuring wild fishing and farm stocks by providing a basis for a future virus resistance breeding program.

Final report

ISBN: 978-0-7300-0303-8
Authors: Adam Miller Madeline Toomey Owen Holland Larry Croft Collin Ahrens Craig Sherman Ary Hoffmann Nick Savva Dean Lisson Andrew Clarke
Final Report • 2021-05-01 • 9.55 MB
2018-057-DLD.pdf

Summary

Abalone viral ganglioneuritis (AVG) remains a significant threat to the economic viability and stability of the Abalone industry in south-eastern Australia. Consequently, there is an urgent need for strategic research aimed at determining the likely vulnerability of fisheries to future AVG outbreaks and providing managers with the necessary tools for biosecuring wild and farmed stocks at regional, state and national scales. The fact that some animals from AVG  affected wild stocks survived the disease outbreak in the early 2000s suggests they were either fortunate enough to have avoided coming into contact with the virus or are genetically resistant to the disease. A research program aimed at characterising AVG resistance in Australian wild Abalone fisheries is expected to provide benefits to wild and farm fisheries at a national scale. If AVG resistance is present in wild Abalone stocks, and its genetic basis can be
characterised, there may be opportunities to:
1) Improve industry knowledge of the mechanisms for animal persistence in virus affected Abalone fisheries and on the resilience of Abalone fisheries to environmental change;
2) Screen wild fishing stocks across all wild fisheries to determine the spatial prevalence of resistant genotypes and to help identify stocks are likely to be resilient or vulnerable to AVG reemergence;
3) Explore options for biosecuring wild stocks through the movement of AVG resistant genotypes to ‘AVG vulnerable’ stocks as part of future restocking and translocation activities; and
4) Establish AVG resistant breeding lines for biosecuring farm stocks across all states of Australia.
In this study we performed a genome wide association study on the Blacklip Abalone (Haliotis rubra) using pooled whole genome re-sequencing data from 343 H. rubra specimens representing 14 Victorian fishing stocks varying in historical AVG exposure. Analyses identified approximately 25,000 SNP loci associated with AVG exposure, many of which mapped to genes known to be involved in herpesvirus response pathways and general virus-host interactions in Haliotids and other animal systems. Most notably, candidate loci mapped to 24 genes known to be associated with Haliotid herpesvirus-1 (HaHV-1)
immunity in the New Zealand pāua (H. iris). Experimental trials are now needed to validate if, and how much, resistance is determined by the candidate genotypes identified in this study before the industry can harness them for management purposes with confidence. This study provides the first line of
evidence of genetic changes and adaptive responses in AVG affected H. rubra fisheries. These findings have potential implications for future management which are discussed in detail but will require further investigation.
Industry
PROJECT NUMBER • 2017-225
PROJECT STATUS:
COMPLETED

Improving risk management of paralytic shellfish toxins (PST) in the Blacklip Abalone (Haliotis rubra rubra)

The accumulation of paralytic shellfish toxins (PST) of microalgal origin in abalone tissues causes a trade and human health risk that requires active management. Toxic algal blooms of the genus Alexandrium have recently caused several abalone harvest closures on the east coast of Australia. Risk...
ORGANISATION:
SARDI Food Safety and Innovation
Industry
PROJECT NUMBER • 2017-220
PROJECT STATUS:
COMPLETED

Testing established methods of early prediction of genetic merit in abalone broodstock

This report provides an assessment of the utility of RNA/DNA ratio as a method for early prediction of high performing abalone broodstock. The study was carried out on farmed Greenlip Abalone (Haliotis laevigata) whereby families were produced and resulting progeny were reared using commercial...
ORGANISATION:
James Cook University (JCU)
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