Abalone Viral Ganglioneuritis (AVG) R&D Needs Workshop
Abalone Viral Ganglioneuritis (AVG) disease is caused by a herpesvirus (infection with abalone herpesvirus, AbHV) and was first detected in Victoria in December 2005. The last recorded instance of this disease in Victoria was in January 2010 at Cape Otway.
The disease was again confirmed in the Cape Nelson area on 2 May 2021. Since then, further detections have been made at several locations and has been confirmed in Victoria as far east as Cape Otway and as far west as the Discovery Bay Marine Park.
While the virus is active there is a need for the Victorian wild catch abalone fishers to urgently review past research, to inform a gap analysis and further research.
This review will support those involved in the outbreak and inform fellow members nationally with a summary of past research and a way forward with other research for up-to-date biosecurity protocols, surveillance, and diagnostic tools to aid rapid detection to future proof the industry. Relevant industry, government representatives and stakeholders will be kept informed and asked to comment and contribute issues and ideas for a cooperative structured way forward to build a common framework on which to establish a set of key actions and or priorities. At the end of a staged process a workshop will be organised to evaluate and reassess research needs, assess and implement research findings and if further gaps are identified develop a framework to action.
Workshop
Investigating the use of a remote operated underwater vehicle (ROUV) for surveying abalone on reefs in the Western zone abalone fishery, Victoria.
Provide a tactical and strategic response to the recent outbreak. Develop a cost effective assessment tool to validate the spread of the virus and build a long term mechanism to undertake fisheries independent surveys.
Indicators for density and biomass of exploitable abalone – developing and applying a new approach
Australian Abalone Growers Association Strategic R&D Plan 2020-2025
The use of dietary intervention with Ulva to improve survival associated with the incidence of summer mortality on farmed Abalone
To improve industry productivity the AAGA need to identify new and improved diets to reduce the impact of summer mortality on the survival of cultured abalone in Australia.
Final report
The dietary inclusion of 10% dried Ulva sp. meal did not appear to lead to any significant alteration in digestive tract or gill structure. Heat stress, in combination with commercial culture stressors, appeared to be a factor associated with histopathological alterations observed in abalone during the study, which were chiefly noted in the gill leaflet tips of Tiger Abalone. Gill leaflet tip damage scores appeared to be useful indicators of damage associated with the combination of culture stressors and heat stress in abalone.
In the Yumbah Narrawong trial, which was carried out during mild summer growing conditions, the dietary inclusion of 10% dried Ulva sp. meal improved growth (SGR increased by 9.6% combined with economically irrelevant differences in survival), feed and nutrient utilisation and live biomass yield for a mixed population of Tiger and Greenlip Abalone and compared to the commercial diet ultimately led to a $729 or 8.4% increase in basic sales revenue per slab tank for the 166-d trial period. Unfortunately, due to logistical constraints due to COVID-19, growth performance data were not available from JTA Avalon trial.
Data produced by this project support the concept that diets for improved abalone production should not be formulated on a least cost basis, but rather on an ingredient quality, and abalone and economic performance basis. Feeds formulated on this basis may support improved profitability.
Application of a machine learning approach for effective stock management of farmed abalone
Determining the number and size distribution of abalone present at various stages of production is critical information for effective stock management. Currently the Australian abalone aquaculture industry spends in the order of $25,000 per annum, per farm, gathering this information by hand. However, the resulting data is of mediocre quality, is limited in its scope, and collecting the data causes stress to the animals (as it is removed from the water) which can compromise growth and survival. Automated counting and measuring of abalone will increase farm efficiency and productivity in the short term and, in the longer term, will provide an advanced platform for further R & D improvements including accurate data collection during experimental trials (e.g. feeds, temperature). Artificial intelligence and machine learning has now matured to a point that accurately counting and measuring abalone is possible using this approach, however specific application to the abalone industry is yet to be achieved. This project would involve the development, training and validation of a machine learning model to identify, segment and measure quantitative abalone traits in production systems and, render the product data to be accessible and applicable for farmers.
Final report
Risk factors and management strategies associated with summer mortality in Australian abalone
Larval dispersal for Southern Rock Lobster and Longspined sea urchin to support management decisions
Seafood Marketing Symposium 2020
Abalone diver observation collection, analysis and reporting system for improved management decision making
Benchmarking for health and productivity in aquaculture
Population genomic assessment of Australian Blacklip Abalone for abalone viral ganglioneuritis (AVG) resistance
AVG remains the greatest threat to the economic viability and stability of the abalone industry in south-eastern Australia. As a consequence, there is an urgent need for strategic research aimed at determining the likely vulnerability of fisheries to future AVG outbreaks and providing managers with the necessary tools for biosecuring wild and farmed stocks at regional, state and national scales.
The fact that some animals from AVG affected wild stocks survived the disease outbreak suggests they were either fortunate enough to have avoided coming into contact with the virus or are genetically resistant to the disease. A research program aimed at characterising AVG resistance in Australian wild abalone fisheries is expected to provide benefits to wild and farm fisheries at a national scale. If AVG resistance is present in wild abalone stocks, and its genetic basis can be characterised, there will be unique opportunities to:
1) Rapidly and cost-effectively screen stocks across all wild fisheries to determine the spatial prevalence of resistant genotypes and to gain an understanding of how biosecure wild abalone stocks are likely to be in the event of AVG re-emergence
2) Biosecure wild stocks through the movement of animals from ‘AVG resistant’ to ‘AVG vulnerable’ stocks as part of future restocking and translocation activities
3) Biosecure farm stocks across all states of Australia through the establishment of an AVG resistance breeding program
This project involves a direct partnership with AAGA, ACA and VFA and is expected to provide much needed insights into the vulnerability of abalone stocks to future AVG outbreaks, and the tools needed to bolster the biosecurity of wild and farmed abalone stocks. As a result, this project has the potential to dramatically improve the economic viability of this rapidly expanding industry.
Final report
characterised, there may be opportunities to:
1) Improve industry knowledge of the mechanisms for animal persistence in virus affected Abalone fisheries and on the resilience of Abalone fisheries to environmental change;
2) Screen wild fishing stocks across all wild fisheries to determine the spatial prevalence of resistant genotypes and to help identify stocks are likely to be resilient or vulnerable to AVG reemergence;
3) Explore options for biosecuring wild stocks through the movement of AVG resistant genotypes to ‘AVG vulnerable’ stocks as part of future restocking and translocation activities; and
4) Establish AVG resistant breeding lines for biosecuring farm stocks across all states of Australia.
In this study we performed a genome wide association study on the Blacklip Abalone (Haliotis rubra) using pooled whole genome re-sequencing data from 343 H. rubra specimens representing 14 Victorian fishing stocks varying in historical AVG exposure. Analyses identified approximately 25,000 SNP loci associated with AVG exposure, many of which mapped to genes known to be involved in herpesvirus response pathways and general virus-host interactions in Haliotids and other animal systems. Most notably, candidate loci mapped to 24 genes known to be associated with Haliotid herpesvirus-1 (HaHV-1)
immunity in the New Zealand pāua (H. iris). Experimental trials are now needed to validate if, and how much, resistance is determined by the candidate genotypes identified in this study before the industry can harness them for management purposes with confidence. This study provides the first line of
evidence of genetic changes and adaptive responses in AVG affected H. rubra fisheries. These findings have potential implications for future management which are discussed in detail but will require further investigation.