SCRC: RTG: Analysis of gene expression and function involved with fat deposition in Yellowtail Kingfish, using RNA-seq data, NOFIMA, Norway
SCRC: RTG: Laboratory visit to be trained to analyse oyster (Sydney Rock Oysters) histology sections, Elizabeth Macarthur Agricultural Institute, NSW
SCRC: Development of germ cell transplantation technology for the Australian aquaculture industry
Currently SBT is being bred in an expensive on-shore facility at Arno Bay, where a single tank holds a limited number of broodstock, which spawn for a limited period of time. In order to expand on the
production of SBT seed, additional facilities/tanks at the costs of millions of dollars will be required and
sourcing additional 12 year old broodstock. Therefore there is a need to look at alternative approaches
for SBT broodstock management. This proposal explores the application of a highly innovative
approach - that is the use of fish surrogates to produce SBT. By identifying the right surrogate for SBT
and developing the specific know-how with respect to optimal germ cell management, SBT seed could
be produced in a fast maturing small host. This would completely overcome the need for large,
expensive broodstock facilities and long term holding of broodstock, while ensuring a continuous
supply of SBT seed, which is much needed for larval rearing R&D and commercialization. This
application relates to the overall investment in closing of the life-cycle of SBT.
Final report
The project was designed to explore the application of surrogate technology as an alternative broodstock system for the Southern Bluefin Tuna (SBT). Surrogate technology, also known as germ cell transplantation, uses germ cells from a donor species, in this case SBT, and transplants them into a host species, the surrogate. The germ cells can migrate and form part of the host’s gonad, resulting in the production of the donor sperm and egg by the host gonad.
We have explored the suitability of the Yellowtail Kingfish (YTK) as a surrogate for SBT. Over 12,000 YTK larvae were injected with SBT germ cells, and 3-4 weeks after transplantation we could observe the migration and colonisation of the SBT cells (which are labelled with red florescence dye for ease of detection) to the YTK genital ridge, confirming SBT cells responded to YTK migration cues. Transplanted larvae were raised and samples assessed a few months later, however so far we could not detect SBT cells in the maturing YTK, indicating that whilst SBT germ cells respond to the YTK migration cues we cannot confirm proliferation of the germ cells in the YTK host at this stage. About 100 YTK approaching one year of age are maintained at CST and will need to be examined for the presence of SBT sperm or eggs once they reach sexual maturity.
SCRC: Masters: Strategic Planning Practices used by Australian Wild Harvest Fishers
SCRC: PhD : An investigation of pathogenic bacterial populations in Atlantic Salmon (Salmo salar L.).
In line with the pervasiveness of gut related illnesses, a recent study by the Tasmanian Institute of Agricultural Research (TIAR) on microbial communities of the hindgut of salmonid fish has shown that these microbial ecosystems are heavily influenced by the local aquatic environment and to some extent diet formulations (Bowman et al 2006). According to this report the intensive feed regime combined with local environmental conditions can lead to overgrowth of certain bacterial species within the gut. The higher number of bacteria in fish hindgut during summer time compared to autumn suggests that the temperature may be instrumental in promoting an overgrowth of pathogenic species. The activity of these bacteria may then lead to a suboptimal feeding and health of the salmon. Clinically this is associated with loss of appetite and reduction in growth rate and a diarrhoeal-like faecal excretion is observed (Bowman et al 2006).
Little is known about the exact pathogenic mechanisms used by these bacteria to cause disease and there is a real need to alleviate pressure on the aquaculture industry from diseases caused by these bacterial species. This project as shown in the objectives is to be run parallel to another project recently accepted by the seafood CRC in providing information on these pathogenic bacteria to be used for testing the efficacy of any isolated probiotics.