SCRC: SCRC Honours Scholarship H4.10 - Impact of fish oil replacement on the expression of antioxidant genes and genes involved in the synthesis of highly unsaturated omega-3 fatty acids in YTK liver and muscle tissue. (Dr Kathy Schuller; Student Nathan Rout-Pitt)
SCRC: SCRC Honours Scholarship H4.6 Effect of temperature and plant protein inclusion on the growth factor gene expression in Yellowtail Kingfish (Jian Qin; Student Geoffrey Collins)
SCRC: SCRC Honours Scholarship H4.5 Improved Yellowtail Kingfish Growth Response, Flesh Quality and PUFA content through Biorefinery Microalgae LC-PUFA Aqua-Feed Additives (Prof Wei Zhang; Student Kopano Maso Machailo)
SCRC: SCRC Honours Scholarship H4.3 The effects of feeding alternative protein sources on the intestinal mucus layer and mucosal architecture in Yellowtail Kingfish (Rebecca Forder; Student Matthew Bransemer.)
SCRC: SCRC Honours Scholarship H4.2 Australian Farmed Prawns:B2B and B2C: A gender comparison of perceptions of relationship marketing (Dr Leone Cameron; Student Hannah O'Brien)
SCRC: SCRC Honours Scholarship H4.1 Sustaining Australia’s aquaculture competitiveness by developing technological advances in genetics: Discovery of functional genes for commercial traits.(Abigail Elizur; Student Nicole Ertl)
Final report
Body colouration, an important survival, mate selection and communication mechanism for animals in the wild, has also significant commercial implications. In aquaculture, a darker body colour in prawns can increase farm profitability by AU$ 2-4 dollar per kilo of prawns. Therefore, there is a strong commercial interest in increasing colour intensity of prawns grown in captivity. In this study, the focus was on F. merguiensis, and the determination of factors that could be involved in colour formation in this species.
Molecular techniques were employed to clone and isolate crustacyanin subunits, genes known to be responsible for colouration in other crustacean species, from the muscle/cuticle tissue of F. merguiensis prawns and to develop gene specific primers to quantify the levels of crustacyanin gene expression in the cuticle of prawns displaying three different colour phenotypes (albino, light and dark).
The sequences encoding for the crustacyanin subunits A and C were isolated from the cuticle tissue in F. merguiensis and their expression levels characterised in prawns displaying different colouration patterns.